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Antibody Name
mAb-4B7
RRID:AB_2728658 RRID Copied  
PDF Report How to cite
Andrew Blagborough; Imperial College London Cat# mAb-4B7, RRID:AB_2728658
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Antibody Information

URL: http://antibodyregistry.org/AB_2728658

Proper Citation: Andrew Blagborough; Imperial College London Cat# mAb-4B7, RRID:AB_2728658

Target Antigen: ookinete surface protein Pfs25

Host Organism: mouse

Clonality: monoclonal

Comments: "mAb 4B7 was produced by fusion of spleen cells from a BALB/c mouse immunized twice with recombinant vaccinia virus that were expressing Pfs25, and boosted with whole P.faki~rum gametes. Supernatants from the fusion were screened for specificity to Pfs25 by immunoblot of reduced Pfs25-B (D. C. Kaslow, unpublished observations). Antisera P7 was produced by pooling sera from mice immunized individually with the peptides SLKCLKENETCKAVD, LKENETCKAVDGIYK, KAVDGIYKCDCKDGF, GIYKCDCKDGFIIDN, and DCKDGFIIDNESSI, which are derived from the fourth EGF-like domain of Pfs25."

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Authentication Plan

dkNET can assist you in preparing authentication plans for antibodies to comply with the NIH Submission Policy. The information can be used while planning your experiments or submitting grant applications. The authentication plan for antibodies is based on methods suggested in "A proposal for validation of antibodies" (Uhlen M et. al., 2016), the guideline published in the Journal of Comparative Neurology (Saper C, 2005)(2), and the example " Authentication of of Key Biological and/or Chemical Resources "(Bandrowski A). For best practices for authenticating antibodies, check our Authentication Reports services.