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Luis Mercado Lab Pontificia Universidad Católica de Valparaíso; Chile Cat# IL-1beta, RRID:AB_2716269Copy Citation Copied
URL: http://antibodyregistry.org/AB_2716269
Proper Citation: Luis Mercado Lab Pontificia Universidad Católica de Valparaíso; Chile Cat# IL-1beta, RRID:AB_2716269
Target Antigen: Interleukin 1 beta
Host Organism: mouse
Clonality: polyclonal
Comments: Schmitt et al, 2015 "Polyclonal antibodies were generated against a mix of 3 synthetic antigenic epitopes (ANKEQLKSISQQSD, QKAAPNRLTTFTIQRHN and DLLNFLLESAVEEHI) from a deduced IL-1β coding sequence [GenBank AJ223954] in New Zealand rabbits... For antibody production, animals were subcutaneously injected at 1, 7 and 14 days with 150 µg immunogen diluted 1:1 in FIS as a T helper cell activator, as previously described (Bethke et al., 2012) and 1:1 in Freund's adjuvant (Thermo). The antiserum was collected on day 18, centrifuged at 700 g for 10 min and the supernatant was stored at −20 °C."
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