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URL: http://antibodyregistry.org/AB_2699340
Proper Citation: StressMarq Biosciences Cat# SMC-213D, RRID:AB_2699340
Target Antigen: Ubiquitin
Host Organism: mouse
Clonality: recombinant monoclonal
Comments:
Applications: WB, IHC, ICC/IF, ELISA
Certification: A 1:5000 dilution of SMC-213 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Purification: Gel filtration
The following antibodies were determined to be duplicates and consolidated by curator on 7/2018: AB_2570420, AB_2699340.
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Source: Antibody Registry (RRID:SCR_006397)
Description: Public registry of antibodies with unique identifiers for commercial and non-commercial antibody reagents to give researchers a way to universally identify antibodies used in publications. The registry contains antibody product information organized according to genes, species, reagent types (antibodies, recombinant proteins, ELISA, siRNA, cDNA clones). Data is provided in many formats so that authors of biological papers, text mining tools and funding agencies can quickly and accurately identify the antibody reagents they and their colleagues used. The Antibody Registry allows any user to submit a new antibody or set of antibodies to the registry via a web form, or via a spreadsheet upload.
URL: http://antibodyregistry.org/
dkNET can assist you in preparing authentication plans for antibodies to comply with the NIH Submission Policy. The information can be used while planning your experiments or submitting grant applications. The authentication plan for antibodies is based on methods suggested in "A proposal for validation of antibodies" (Uhlen M et. al., 2016), the guideline published in the Journal of Comparative Neurology (Saper C, 2005)(2), and the example " Authentication of of Key Biological and/or Chemical Resources "(Bandrowski A). For best practices for authenticating antibodies, check our Authentication Reports services.
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