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Antibody Name
BOULE / BOLL antibody
RRID:AB_10733167 RRID Copied  
PDF Report How to cite
GeneTex Cat# GTX89597, RRID:AB_10733167
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Antibody Information

URL: http://antibodyregistry.org/AB_10733167

Proper Citation: GeneTex Cat# GTX89597, RRID:AB_10733167

Target Antigen: BOULE / BOLL antibody

Host Organism: goat

Clonality: polyclonal

Comments: Discontinued; manufacturer recommendations: ELISA; Preliminary experiments gave an approx 22kDa band in Human Testis lysates after 1.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.6kDa according to NP_932074.1 and 31.3kDa according to NP_149019.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. However, literature reports a 36kDa band in Human and Mouse Testes (PMID: 11390979 ). Have any further splice variants/modified forms been reported? This antibody is expected to recognize both reported isoforms (NP_932074.1; NP_149019.1). ELISA.

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Discontinued
Discontinued at GeneTex

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Data and Source Information
Authentication Plan

dkNET can assist you in preparing authentication plans for antibodies to comply with the NIH Submission Policy. The information can be used while planning your experiments or submitting grant applications. The authentication plan for antibodies is based on methods suggested in "A proposal for validation of antibodies" (Uhlen M et. al., 2016), the guideline published in the Journal of Comparative Neurology (Saper C, 2005)(2), and the example " Authentication of of Key Biological and/or Chemical Resources "(Bandrowski A). For best practices for authenticating antibodies, check our Authentication Reports services.