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URL: https://web.expasy.org/cellosaurus/CVCL_ZV93
Proper Citation: RRID:CVCL_ZV93
Description: Cell line MDCK Actinin-M-cpstFRET is a Spontaneously immortalized cell line with a species of origin Canis lupus familiaris (Dog)
Sex: Female
Defining Citation: PMID:25422450
Comments: Characteristics: Used to report forces in actinin in living cells. Has one actinin molecule with the cpstFRET sensor incorporated between spectrin repeat domains 3 and 4 toward the middle of actinin. The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency.
Category: Spontaneously immortalized cell line
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Source: Cellosaurus
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