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URL: https://web.expasy.org/cellosaurus/CVCL_A1TV
Proper Citation: RRID:CVCL_A1TV
Description: Cell line EndoC-betaH21-PGF2AOF is a Conditionally immortalized cell line with a species of origin Homo sapiens (Human)
Sex: Sex unspecified
Defining Citation: PMID:25259951
Comments: Virology: Contains an integrated xenotropic MuLV-related virus (XMRV) Bxv-1 (from parent cell line EndoC-betaH2)., Characteristics: In this cell line the Fucci probe was produced by transducing with a construct LTR-PuroR-IRES-mAG/GMNN-2A-mKO2/CDT1-LTR., Characteristics: Transduced with the Fucci probe which consist of the fusion of monomeric Kusabira-Orange2 (mKO2) to the ubiquitination domain of human CDT1 (AA 30-120) and monomeric AzamiGreen (mAG) to the ubiquitination domain of human geminin (AA 1-110)., Characteristics: The lentiviral vector insert containing the SV40 LT and hTERT can be excised by the Cre recombinase upon addition of tamixofen. Following excision of immortalizing transgenes, cell proliferation sharply decreases and in parallel there is a massive enhancement of beta cell-specific features such as increased INS gene expression.
Category: Conditionally immortalized cell line
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Source: Cellosaurus
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