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URL: http://www.addgene.org/22776
Proper Citation: RRID:Addgene_22776
Insert Name: CreERT2
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19903759
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pMSCV puro; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Cre-ERT2 contains Cre fused to the ligand-binding domain of a mutated estrogen receptor, which recognizes tamoxifen or its derivative 4-hydroxytamoxifen(4-OHT). This construct permits tamoxifen-dependent Cre activity. Please note that Addgene's sequencing results identified a K183E mutation in the Cre protein sequence. This mutation was not intentional, but was present in the original plasmid and according to the depositing laboratory the plasmid functions as described in the associated publication.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
