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Norway

PMID:34429103  

An economical and highly adaptable optogenetics system for individual and population-level manipulation of Caenorhabditis elegans.

M Koopman | L Janssen | E A A Nollen
BMC biology | 2021

Optogenetics allows the experimental manipulation of excitable cells by a light stimulus without the need for technically challenging and invasive procedures. The high degree of spatial, temporal, and intensity control that can be achieved with a light stimulus, combined with cell type-specific expression of light-sensitive ion channels, enables highly specific and precise stimulation of excitable cells. Optogenetic tools have therefore revolutionized the study of neuronal circuits in a number of models, including Caenorhabditis elegans. Despite the existence of several optogenetic systems that allow spatial and temporal photoactivation of light-sensitive actuators in C. elegans, their high costs and low flexibility have limited wide access to optogenetics. Here, we developed an inexpensive, easy-to-build, modular, and adjustable optogenetics device for use on different microscopes and worm trackers, which we called the OptoArm.

Pubmed ID: 34429103

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Associated grants

  • Agency: Cancer Research UK, United Kingdom
    Id: C12292/A11174
  • Agency: Cancer Research UK, United Kingdom
    Id: C1281/A12014
  • Agency: Cancer Research UK, United Kingdom
    Id: C5047/A10692
  • Agency: Cancer Research UK, United Kingdom
    Id: C8197/A16565
  • Agency: NCI NIH HHS, United States
    Id: U19 CA148065
  • Agency: Cancer Research UK, United Kingdom
    Id: C5047/A15007
  • Agency: CIHR, Canada
    Id: GPH-129344
  • Agency: NCI NIH HHS, United States
    Id: R01 CA128978
  • Agency: Cancer Research UK, United Kingdom
    Id: C1287/A10118
  • Agency: NCI NIH HHS, United States
    Id: U19 CA148537
  • Agency: NCI NIH HHS, United States
    Id: U19 CA148112
  • Agency: Cancer Research UK, United Kingdom
    Id: C1287/A10710
  • Agency: Cancer Research UK, United Kingdom
    Id: C5047/A8384
  • Agency: Cancer Research UK, United Kingdom
    Id: C1287/A16563

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Fiji (tool)

RRID:SCR_002285

Software package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.

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RRID:SCR_010279

Vector graphics software to create digital graphics, illustrations, and typography for several types of media: print, web, interactive, video, and mobile.

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ggplot2 (tool)

RRID:SCR_014601

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