Searching the Resource Information Network

Our searching services are busy right now. Please try again later

  • Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes
Norway

PMID:29161344  

  1. Home
  2. PMID:29161344

Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.

Jianjun Hu | Fengyun Sun | Mary Ann Handel
Biology of reproduction | 2018

Eukaryotic translation initiation factor 4G (EIF4G) is an important scaffold protein in the translation initiation complex. In mice, mutation of the Eif4g3 gene causes male infertility, with arrest of meiosis at the end of meiotic prophase. This study documents features of the developmental expression and subcellular localization of EIF4G3 that might contribute to its highly specific role in meiosis and spermatogenesis. Quite unexpectedly, EIF4G3 is located in the nucleus of spermatocytes, where it is highly enriched in the XY body, the chromatin domain formed by the transcriptionally inactive sex chromosomes. Moreover, many other, but not all, translation-related proteins are also localized in the XY body. These unanticipated observations implicate roles for the XY body in controlling mRNA metabolism and/or "poising" protein translation complexes before the meiotic division phase in spermatocytes.

Pubmed ID: 29161344

Research resources used in this publication

Additional research tools detected in this publication

None found

Antibodies used in this publication

Associated grants

  • Agency: NICHD NIH HHS, United States
    Id: R01 HD073077
  • Agency: National Cancer Institute, United States
    Id: P30 CA034196

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.

gamma Tubulin (C-20) (antibody)

RRID:AB_2211262

This polyclonal targets TUBG1, TUBG2

View all literature mentions

Ribosomal Protein L28 (A-16) (antibody)

RRID:AB_2181749

This polyclonal targets RPL28

View all literature mentions

eIF4G2/p97 (D88B6) XP Rabbit mAb (antibody)

RRID:AB_10622189

This monoclonal targets eIF4G2/p97 (D88B6) XP Rabbit mAb

View all literature mentions

eIF4E Monoclonal Antibody (5D11) (antibody)

RRID:AB_2536738

This monoclonal targets eIF4E

View all literature mentions

Phospho-4E-BP1 (Thr70) Antibody (antibody)

RRID:AB_330949

This polyclonal targets Phospho-4E-BP1 (Thr70)

View all literature mentions

Anti-α-Tubulin antibody (antibody)

RRID:AB_477593

This monoclonal targets α-Tubulin

View all literature mentions

eIF4A3 antibody (antibody)

RRID:AB_732124

This polyclonal targets Human eIF4A3

View all literature mentions

eIF4A1 Monoclonal Antibody (K.213.2) (antibody)

RRID:AB_11006110

This monoclonal targets eIF4A1

View all literature mentions

RPS3 Polyclonal Antibody (antibody)

RRID:AB_2545450

This unknown targets RPS3

View all literature mentions

EIF4A2 antibody (antibody)

RRID:AB_2097378

This polyclonal targets EIF4A2

View all literature mentions

4E-BP1 (N-19) (antibody)

RRID:AB_633426

This polyclonal targets EIF4EBP1

View all literature mentions

Monoclonal ANTI-FLAG® M2 antibody produced in mouse (antibody)

RRID:AB_262044

This monoclonal targets FLAG

View all literature mentions

Ribosomal Protein L10a (JK-16) (antibody)

RRID:AB_2285311

This monoclonal targets RPL10A

View all literature mentions

DAZL Antibody (antibody)

RRID:AB_10860078

This polyclonal targets DAZL

View all literature mentions

eIF4G3 Polyclonal Antibody (antibody)

RRID:AB_2548575

This unknown targets eIF4G3

View all literature mentions

Phospho-EIF4EBP1-S111 Antibody (antibody)

RRID:AB_1278020

This polyclonal targets Human EIF4EBP1, phospho (Ser111)

View all literature mentions

Phospho-4E-BP1 (Ser65) Antibody (antibody)

RRID:AB_330947

This polyclonal targets Phospho-4E-BP1 (Ser65)

View all literature mentions

eIF4GI (D6A6) Rabbit mAb (antibody)

RRID:AB_11178378

This monoclonal targets eIF4GI (D6A6) Rabbit mAb

View all literature mentions

eIF2C4 (K-15) (antibody)

RRID:AB_2277670

This polyclonal targets EIF2C4

View all literature mentions

PABPC1-human (antibody)

RRID:AB_777008

This polyclonal targets PABPC1

View all literature mentions

Anti-Puromycin Antibody, clone 12D10 (antibody)

RRID:AB_2566826

This monoclonal targets Puromycin from Streptomyces alboniger

View all literature mentions

BOLL Polyclonal Antibody (antibody)

RRID:AB_2548282

This unknown targets BOLL

View all literature mentions

Phospho-CDK2 (Thr160) Antibody (antibody)

RRID:AB_2078685

This polyclonal targets CDK3

View all literature mentions

Phospho-Chk1 (Ser345) Antibody (antibody)

RRID:AB_330023

This polyclonal targets Phospho-Chk1 (Ser345)

View all literature mentions

Mnk1 (C4C1) Rabbit mAb (antibody)

RRID:AB_2235175

This monoclonal targets Mnk1

View all literature mentions

Goat Anti-Human Musashi-1 Polyclonal antibody, Unconjugated (antibody)

RRID:AB_2147926

This polyclonal targets Human Musashi-1

View all literature mentions

Rabbit Anti-Chk2, phospho (Thr383) Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_942224

This polyclonal targets Chk2 (phospho T383)

View all literature mentions

Rabbit Anti-HuR Polyclonal antibody, Unconjugated (antibody)

RRID:AB_310641

This polyclonal targets HuR

View all literature mentions

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 (antibody)

RRID:AB_309864

This monoclonal targets Histone H2A.X pSer139

View all literature mentions

Translin antibody (antibody)

RRID:AB_1524509

This polyclonal targets Translin

View all literature mentions

eIF4G3/eIF4GII Antibody (antibody)

RRID:AB_1211018

This unknown targets eIF4G3/eIF4GII

View all literature mentions

S6 Monoclonal Antibody (C.896.4) (antibody)

RRID:AB_10999800

This monoclonal targets S6

View all literature mentions

Frizzled 7 antibody (antibody)

RRID:AB_10864171

This polyclonal targets Frizzled 7 antibody

View all literature mentions

ATR (N-19) (antibody)

RRID:AB_630893

This polyclonal targets ATR

View all literature mentions

EIF4E3 antibody (antibody)

RRID:AB_2262162

This polyclonal targets EIF4E3

View all literature mentions

eIF4E2 (YB-18) (antibody)

RRID:AB_1122491

This monoclonal targets eIF4E2 (YB-18)

View all literature mentions

HSPA2 antibody (antibody)

RRID:AB_2119687

This polyclonal targets HSPA2

View all literature mentions

RPS13 antibody (antibody)

RRID:AB_10712319

This polyclonal targets RPS13 antibody

View all literature mentions

Phospho-CDK1 (Tyr15) Monoclonal Antibody (E.658.6) (antibody)

RRID:AB_10984869

This monoclonal targets Phospho-CDK1 (Tyr15)

View all literature mentions

Rabbit Anti-MNK2 Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_1860814

This polyclonal targets MNK2

View all literature mentions

eIF4A1 Monoclonal Antibody (K.213.2) (antibody)

RRID:AB_11006110

This monoclonal targets eIF4A1

View all literature mentions

RPS3 Polyclonal Antibody (antibody)

RRID:AB_2545450

This unknown targets RPS3

View all literature mentions

DAZL Antibody (antibody)

RRID:AB_10860078

This polyclonal targets DAZL

View all literature mentions

Monoclonal ANTI-FLAG® M2 antibody produced in mouse (antibody)

RRID:AB_262044

This monoclonal targets FLAG

View all literature mentions

Phospho-4E-BP1 (Ser65) Antibody (antibody)

RRID:AB_330947

This polyclonal targets Phospho-4E-BP1 (Ser65)

View all literature mentions

4E-BP1 (N-19) (antibody)

RRID:AB_633426

This polyclonal targets EIF4EBP1

View all literature mentions

PABPC1-human (antibody)

RRID:AB_777008

This polyclonal targets PABPC1

View all literature mentions

BOLL Polyclonal Antibody (antibody)

RRID:AB_2548282

This unknown targets BOLL

View all literature mentions

eIF4G3 Polyclonal Antibody (antibody)

RRID:AB_2548575

This unknown targets eIF4G3

View all literature mentions

Phospho-EIF4EBP1-S111 Antibody (antibody)

RRID:AB_1278020

This polyclonal targets Human EIF4EBP1, phospho (Ser111)

View all literature mentions

Goat Anti-Human Musashi-1 Polyclonal antibody, Unconjugated (antibody)

RRID:AB_2147926

This polyclonal targets Human Musashi-1

View all literature mentions

eIF4A3 antibody (antibody)

RRID:AB_732124

This polyclonal targets Human eIF4A3

View all literature mentions

Frizzled 7 antibody (antibody)

RRID:AB_10864171

This polyclonal targets Frizzled 7 antibody

View all literature mentions

Anti-Puromycin Antibody, clone 12D10 (antibody)

RRID:AB_2566826

This monoclonal targets Puromycin from Streptomyces alboniger

View all literature mentions

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 (antibody)

RRID:AB_309864

This monoclonal targets Histone H2A.X pSer139

View all literature mentions

ATR (N-19) (antibody)

RRID:AB_630893

This polyclonal targets ATR

View all literature mentions

Rabbit Anti-Chk2, phospho (Thr383) Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_942224

This polyclonal targets Chk2 (phospho T383)

View all literature mentions

Anti-α-Tubulin antibody (antibody)

RRID:AB_477593

This monoclonal targets α-Tubulin

View all literature mentions

Phospho-Chk1 (Ser345) Antibody (antibody)

RRID:AB_330023

This polyclonal targets Phospho-Chk1 (Ser345)

View all literature mentions

Ribosomal Protein L28 (A-16) (antibody)

RRID:AB_2181749

This polyclonal targets RPL28

View all literature mentions

Translin antibody (antibody)

RRID:AB_1524509

This polyclonal targets Translin

View all literature mentions

eIF4E2 (YB-18) (antibody)

RRID:AB_1122491

This monoclonal targets eIF4E2 (YB-18)

View all literature mentions

HSPA2 antibody (antibody)

RRID:AB_2119687

This polyclonal targets HSPA2

View all literature mentions

Mnk1 (C4C1) Rabbit mAb (antibody)

RRID:AB_2235175

This monoclonal targets Mnk1

View all literature mentions

eIF4A3 antibody (antibody)

RRID:AB_732124

This polyclonal targets Human eIF4A3

View all literature mentions

eIF4G3/eIF4GII Antibody (antibody)

RRID:AB_1211018

This unknown targets eIF4G3/eIF4GII

View all literature mentions

RPS13 antibody (antibody)

RRID:AB_10712319

This polyclonal targets RPS13 antibody

View all literature mentions

eIF4A3 antibody (antibody)

RRID:AB_732124

This polyclonal targets Human eIF4A3

View all literature mentions

EIF4E3 antibody (antibody)

RRID:AB_2262162

This polyclonal targets EIF4E3

View all literature mentions

eIF2C4 (K-15) (antibody)

RRID:AB_2277670

This polyclonal targets EIF2C4

View all literature mentions

Phospho-CDK1 (Tyr15) Monoclonal Antibody (E.658.6) (antibody)

RRID:AB_10984869

This monoclonal targets Phospho-CDK1 (Tyr15)

View all literature mentions

EIF4A2 antibody (antibody)

RRID:AB_2097378

This polyclonal targets EIF4A2

View all literature mentions

Phospho-4E-BP1 (Thr70) Antibody (antibody)

RRID:AB_330949

This polyclonal targets Phospho-4E-BP1 (Thr70)

View all literature mentions

S6 Monoclonal Antibody (C.896.4) (antibody)

RRID:AB_10999800

This monoclonal targets S6

View all literature mentions

eIF4E Monoclonal Antibody (5D11) (antibody)

RRID:AB_2536738

This monoclonal targets eIF4E

View all literature mentions

Rabbit Anti-HuR Polyclonal antibody, Unconjugated (antibody)

RRID:AB_310641

This polyclonal targets HuR

View all literature mentions

Ribosomal Protein L10a (JK-16) (antibody)

RRID:AB_2285311

This monoclonal targets RPL10A

View all literature mentions

gamma Tubulin (C-20) (antibody)

RRID:AB_2211262

This polyclonal targets TUBG1, TUBG2

View all literature mentions

Phospho-CDK2 (Thr160) Antibody (antibody)

RRID:AB_2078685

This polyclonal targets CDK3

View all literature mentions

Rabbit Anti-MNK2 Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_1860814

This polyclonal targets MNK2

View all literature mentions

eIF4G2/p97 (D88B6) XP Rabbit mAb (antibody)

RRID:AB_10622189

This monoclonal targets eIF4G2/p97 (D88B6) XP Rabbit mAb

View all literature mentions

eIF4GI (D6A6) Rabbit mAb (antibody)

RRID:AB_11178378

This monoclonal targets eIF4GI (D6A6) Rabbit mAb

View all literature mentions

About

The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.

Recent News Entries

NIDDK Early Research Partnership Engagement Challenge

Funding Opportunity: National NIH K12 DiabDocs Career Development Program

NIH Data Sharing and Reuse Seminar- Sex Differences in Acute Kidney Injury Risk and Outcomes: Insights from MIMIC-III and MIMIC-IV

Contact Us

Email: info_at_ dknet.org

Stay Connected

About SciCrunch | Privacy Policy | Terms of Service

SciCrunch