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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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Colorimetric RT-LAMP Research group SARS-COV-2 detection protocol Resource Report Resource Website |
Teodor Emilov Mentov | DOI:10.17504/protocols.io.bk59ky96 | XPRIZE Rapid Covid Testing | HUBU | 1 | 2020 | Teodor Emilov Mentov 2020. Colorimetric RT-LAMP Research group SARS-COV-2 detection protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bk59ky96 | 2021-04-15 09:14:21 | ||||
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High-throughput SARS-CoV-2 RNA extraction from nasopharyngeal swabs using repurposed 3D printers. Resource Report Resource Website |
Koen Vandelannoote | DOI:10.17504/protocols.io.bkynkxve | Coronavirus Method Development Community, XPRIZE Rapid Covid Testing, Koen Vandelannoote | Here, we describe a semi-automated workflow for paramagnetic bead-based extraction of SARS-CoV-2 RNA from human upper respiratory samples that uses low-cost repurposed Creality3D Ender-3 3D-printers and readily accessible reagents. The protocol described here is for the use of four instruments in parallel, each processing 24 samples, 96 samples per batch. | University of Melbourne | 1 | 2020 | Koen Vandelannoote 2020. High-throughput SARS-CoV-2 RNA extraction from nasopharyngeal swabs using repurposed 3D printers.. protocols.io https://dx.doi.org/10.17504/protocols.io.bkynkxve | 2021-04-15 09:14:21 | |||
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RAPID RNA Extraction Resource Report Resource Website |
Noah Toppings | DOI:10.17504/protocols.io.bkp8kvrw | XPRIZE Rapid Covid Testing | University of Calgary | 1 | 2020 | Noah Toppings 2020. RAPID RNA Extraction. protocols.io https://dx.doi.org/10.17504/protocols.io.bkp8kvrw | 2021-04-15 09:14:33 | ||||
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Boston Biopharma CareStart™ Rapid Diagnostic Antigen Test Resource Report Resource Website |
tclark , Ahmad Hashem, Jun Yong Ha, Charlie Mize | DOI:10.17504/protocols.io.bkzxkx7n | XPRIZE Rapid Covid Testing | [Boston Biopharma], [Boston Biopharma], Access Bio, Boston Biopharma | 1 | 2020 | tclark , Ahmad Hashem, Jun Yong Ha, Charlie Mize 2020. Boston Biopharma CareStart™ Rapid Diagnostic Antigen Test. protocols.io https://dx.doi.org/10.17504/protocols.io.bkzxkx7n | 2021-04-15 09:14:26 | ||||
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u-Smell-it olfactory test Resource Report Resource Website |
dkt | DOI:10.17504/protocols.io.bkxkkxkw | Coronavirus Method Development Community, XPRIZE Rapid Covid Testing | D. Toomre, K Piironen | 2 | 2020 | dkt 2020. u-Smell-it olfactory test . protocols.io https://dx.doi.org/10.17504/protocols.io.bkxkkxkw | 2021-04-15 09:14:29 | ||||
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COVIDscanDX LAMP Resource Report Resource Website |
Don Cooper | DOI:10.17504/protocols.io.bk4dkys6 | Coronavirus Method Development Community, XPRIZE Rapid Covid Testing | Neuroganics Diagnostics LLC | 1 | 2020 | Don Cooper 2020. COVIDscanDX LAMP. protocols.io https://dx.doi.org/10.17504/protocols.io.bk4dkys6 | 2021-04-15 09:14:29 | ||||
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SensingSelf S4 Multiplex Covid-19 /MERS-CoV/ Influenza A/B Rapid Antigens Test Kit (Saliva/Sputum/Stool) Resource Report Resource Website |
Shripal Gandhi, Santo Purnama, Keyur Patel, Praveen Sukumara, Dr Rinu R Ravi | DOI:10.17504/protocols.io.bkwckxaw | Coronavirus Method Development Community, SensingSelfMission, XPRIZE Rapid Covid Testing | Summary:The S4 Multiplex Covid-19 /MERS-CoV/ Influenza A/B Rapid Antigens Test Kit developed by Sensing Self Pte. Ltd. can quickly and qualitatively detect Novel Coronavirus / Influenza A/ Influenza B /MERS in human saliva/sputum/stool samples. It can be used as an aid for COVID-19 diagnosis. It provides an aid in the diagnosis of infection by the above viruses.Objective:According to the clinical trial plan, the S4 Multiplex Covid-19 /MERS-CoV/ Influenza A/B Rapid Antigen test kit or “test reagent”, is to test saliva/sputum/stool samples from healthy subjects and confirmed patients with infections caused by these viruses. Test results are compared with another commercial Covid-19 /MERS-CoV/ Influenza A/B nucleic acid detection kit, which is defined as the “gold standard”. The sensitivity, specificity, and total agreement rate are used to evaluate the feasibility of the test reagent in clinical applications.Method:A collection of clinical samples were examined by the S4 Multplex Covid-19 /MERS-CoV/ Influenza A/B Rapid Antigen test kit and the gold standard SARS-COV-2/MERS-CoV/ Influenza A/B nucleic acid detection kit in parallel, to calculate the clinical sensitivity, clinical specificity and total agreement rate of the test reagent.Results:For SARS-CoV-2, compared to the gold standard:In Saliva, the clinical sensitivity of test reagent reached 88.3%, the clinical specificity reached 100.0%In Sputum, the clinical sensitivity of test reagent reached 93.3%, the clinical specificity reached 100.0% In Stool, the clinical sensitivity of test reagent reached 93.3%, the clinical specificity reached 100.0%For MERS, compared to the gold standard:In Saliva, the clinical sensitivity of test reagent reached 86%, the clinical specificity reached 100.0%In Sputum, the clinical sensitivity of test reagent reached 90%, the clinical specificity reached 100.0% In Stool, the clinical sensitivity of test reagent reached 90%, the clinical specificity reached 100.0%For Influenza Virus A, compared to the gold standard:In Saliva, the clinical sensitivity of test reagent reached 85.7%, the clinical specificity reached 98.3%In Sputum, the clinical sensitivity of test reagent reached 91.4%, the clinical specificity reached 100.0% In Stool, the clinical sensitivity of test reagent reached 90%, the clinical specificity reached 100.0%For Influenza Virus B, compared to the gold standard:In Saliva, the clinical sensitivity of test reagent reached 82.9%, the clinical specificity reached 100.0%In Sputum, the clinical sensitivity of test reagent reached 87.1%, the clinical specificity reached 96.7% In Stool, the clinical sensitivity of test reagent reached 85.7%, the clinical specificity reached 98.3%Conclusions:The performance of test reagent has a high agreement rate with the gold standard, proving its good feasibility in diagnosing suspected Covid-19 /MERS-CoV/ Influenza A/B cases. | Sensing Self Pte Ltd, Singapore, Sensing Self Pte Ltd, Singapore, Sensing Self Pte Ltd, Singapore, Sensing Self Pte Ltd, Singapore, Sensing Self Pte Ltd, Singapore | http://www.sensingself.me | 1 | 2020 | Shripal Gandhi, Santo Purnama, Keyur Patel, Praveen Sukumara, Dr Rinu R Ravi 2020. SensingSelf S4 Multiplex Covid-19 /MERS-CoV/ Influenza A/B Rapid Antigens Test Kit (Saliva/Sputum/Stool). protocols.io https://dx.doi.org/10.17504/protocols.io.bkwckxaw | 2021-04-15 09:14:39 | ||
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FloodLAMP Inactivation Solution Prep v1.0 Resource Report Resource Website |
Randy True | DOI:10.17504/protocols.io.bk5qky5w | FloodLAMP, XPRIZE Rapid Covid Testing | The Inactivation Solution includes TCEP, EDTA, NaOH, and water. It should be made in a chemical fume hood, as the TCEP is caustic. It is very straightforward to make if starting with liquid-form TCEP. We have not made it with powder-form TCEP, which is about 30% cheaper at low volume retail prices from Sigma. We have yet to do long-term stability studies; however, we think it's fine stored at room temperature for at least a few weeks. If yellow Binding Solution does not go clear upon addition to an inactivated sample or PBS+Inactivation Soln, suspect that the Inactivation Solution may have degraded, and repeat with another aliquot or freshly-made Inactivation Solution.It has also been suggested by Albert Yu of Brandeis has recommended using a 1.4N NaOH concentration for saliva instead of 1.15N NaOH. NaOH should be scale up by 1.4/1.15 and water down for this. | FloodLAMP Biotechnologies | 1 | 2020 | Randy True 2020. FloodLAMP Inactivation Solution Prep v1.0. protocols.io https://dx.doi.org/10.17504/protocols.io.bk5qky5w | 2021-04-15 09:14:36 | |||
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Coronavirus Lateral Flow Assay (LFA) sample preparation protocol Resource Report Resource Website |
peijun he | DOI:10.17504/protocols.io.bksfkwbn | Coronavirus Method Development Community, XPRIZE Rapid Covid Testing, Highfield Diagnostics | Coronavirus Lateral Flow Assay (LFA) sample preparation protocol | Highfield Diagnostics, UK | 1 | 2020 | peijun he 2020. Coronavirus Lateral Flow Assay (LFA) sample preparation protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bksfkwbn | 2021-04-15 09:14:44 | |||
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Thermal cycling of the PCR Resource Report Resource Website |
philippe.bechtold | DOI:10.17504/protocols.io.bk6jkzcn | XPRIZE Rapid Covid Testing | ETHZ - ETH Zurich | 1 | 2020 | philippe.bechtold 2020. Thermal cycling of the PCR. protocols.io https://dx.doi.org/10.17504/protocols.io.bk6jkzcn | 2021-04-15 09:14:31 | ||||
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Doctor Vida Pocket - COVID-19 assay - Instructions for Use Resource Report Resource Website |
goncalo.doria | DOI:10.17504/protocols.io.bkc8kszw | XPRIZE Rapid Covid Testing | Instruction of use for Doctor Vida pocket - COVID-19 assay | STAB VIDA Lda | 1 | 2020 | goncalo.doria 2020. Doctor Vida Pocket - COVID-19 assay - Instructions for Use. protocols.io https://dx.doi.org/10.17504/protocols.io.bkc8kszw | 2021-04-15 09:14:48 | |||
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Canary VOC testing protocol Resource Report Resource Website |
Charlie Mize, Raj Reddy, Purav Badani | DOI:10.17504/protocols.io.bkm4ku8w | Canary, XPRIZE Rapid Covid Testing | Canary Health Technologies, Canary Health Technologies, Canary Health Technologies | 1 | 2020 | Charlie Mize, Raj Reddy, Purav Badani 2020. Canary VOC testing protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bkm4ku8w | 2021-04-15 09:14:34 | ||||
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Mirimus COVID-19 Pool Surveillance RT-PCR Testing Resource Report Resource Website |
Prem Premsrirut, Ana Vasileva, Huiting Cheng | DOI:10.17504/protocols.io.bkv3kw8n | Coronavirus Method Development Community, XPRIZE Rapid Covid Testing | OverviewAt the start of the Pandemic in the US, New York was hit with more cases of COVID-19 than any single country in the world. Although case numbers are finally at an all-time low, the question of how to safely reopen the economy, school and businesses still remains unclear. Today, SARS-CoV-2 detection via RT-PCR methods remains the gold standard to detect active infection and recommend isolation of individuals in order to prevent further spread. Unfortunately, the cost and bottlenecks created from repeated massive testing efforts have become unsustainable. As a way to address this issue, we have devised a strategy of pooling samples and testing entire populations together as one unit. This cuts the cost of testing dramatically and still enables the detection of a susceptible group that would need to undergo individual testing.To meet the COVID-19 testing demands, we created an organizational-based pooling strategy, whereby organizations can enroll in surveillance testing to monitor groups of people through pooled testing. By using organizational-based pooling, we avoid random pooling of samples. Instead, we pool groups of known contacts from a defined workplace, school or organization where people regularly interact. Therefore, a positive case would likely have an impact on the entire pooled population and the entire pooled population can be treated as an infected cohort until further individual testing, potentially, through a healthcare provider is recommended or offered.Method of Pooled TestingFor pooled surveillance testing to be effective and widely adopted, sample collection methods must also be simplified and non-invasive in order to achieve high compliance and enrollment into a surveillance program. The method of testing must also be highly sensitive to accurately detect one positive case in a large pool of negative samples. For these reasons, we developed a simple saliva collection method with a validated pooled saliva specimens using RT-PCR and an acceptable limit of detection, which will be the most sensitive diagnostic test to date. We established automated methods to pool up to 24 saliva specimens and if a positive case is detected, the pools can be further broken down to pools of 2 specimens. The remaining 2 individuals within a pool will be referred to or, potentially, offered further clinical diagnostic testing by a healthcare provider. | Mirimus, Inc., Mirimus, Inc., Mirimus, Inc. | 1 | 2020 | Prem Premsrirut, Ana Vasileva, Huiting Cheng 2020. Mirimus COVID-19 Pool Surveillance RT-PCR Testing. protocols.io https://dx.doi.org/10.17504/protocols.io.bkv3kw8n | 2021-04-15 09:14:52 | |||
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XPRIZE SHINE - In-tube Fluorescent SARS-CoV-2 Saliva Test Resource Report Resource Website |
Jon Arizti-Sanz, Catherine A. Freije, Chloe K. Boehm, Sameed M. Siddiqui, Allen M. Goodman, Tinna-Solveig F. Kosoko-Thoroddsen, A'Doriann Y. Bradley, Jeremy Johnson, Pardis C. Sabeti, Cameron Myhrvold | DOI:10.17504/protocols.io.bk3rkym6 | XPRIZE Rapid Covid Testing | This protocol describes how to perform a SHINE in-tube fluorescent assay to detect SARS-CoV-2 RNA from a self-collected nasopharyngeal sample. This protocol is intended for point-of-care use. All enzymatic components are provided as a single-test freeze-dried pellet for shelf-stable storage, and all steps of the protocol are performed at ambient temperature. The protocol requires a transilluminator or another equivalent blue light emitting device. The protocol presented here is an improved version of the method presented in Arizti-Sanz J*, Freije CA*, et al. Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2. bioRxiv (2020). | Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Harvard-MIT Program in Health Sciences and Technology, 77 Massachusetts Ave., Cambridge, MA 02139, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Computational and Systems Biology PhD Program, MIT, Cambridge, MA 02139, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Harvard T.H. Chan School of Public Health, 677 Huntington Avenue, Boston, MA 02115, USA.; Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA.; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA. | 1 | 2020 | Jon Arizti-Sanz, Catherine A. Freije, Chloe K. Boehm, Sameed M. Siddiqui, Allen M. Goodman, Tinna-Solveig F. Kosoko-Thoroddsen, A'Doriann Y. Bradley, Jeremy Johnson, Pardis C. Sabeti, Cameron Myhrvold 2020. XPRIZE SHINE - In-tube Fluorescent SARS-CoV-2 Saliva Test. protocols.io https://dx.doi.org/10.17504/protocols.io.bk3rkym6 | 2021-04-15 09:15:17 | |||
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BW SARS-CoV-2 Laboratory Test Resource Report Resource Website |
Ivan Rueda, peter.marx | DOI:10.17504/protocols.io.bkztkx6n | XPRIZE Rapid Covid Testing, Biology Works LLC | This protocol is the laboratory-based equivalent of the LAMP assay used for detecting SARS-CoV-2. A similar protocol is performed automatically within the Biology Works LLC at-home device. | Biology Works LLC, Biology Works LLC | 1 | 2020 | Ivan Rueda, peter.marx 2020. BW SARS-CoV-2 Laboratory Test. protocols.io https://dx.doi.org/10.17504/protocols.io.bkztkx6n | 2021-04-15 09:15:21 | |||
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AMDx Lateral Flow Assay WIth a Cover Protocol Resource Report Resource Website |
samz | DOI:10.17504/protocols.io.bk54ky8w | Coronavirus Method Development Community, XPRIZE Rapid Covid Testing | American Molecular Dx LLC | 1 | 2020 | samz 2020. AMDx Lateral Flow Assay WIth a Cover Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bk54ky8w | 2021-04-15 09:15:03 | ||||
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OnsiteGene 3 Protocol Nasal Extract Resource Report Resource Website |
yliu | DOI:10.17504/protocols.io.bkugkwtw | OnsiteGene 1, XPRIZE Rapid Covid Testing | This OnsiteGene protocol is designed for testing the nasal swab samples with a superfast nucleic acid extraction procedure and a superfast real-time PCR procedure. It uses the Star Array® Hi-SenseTM COVID-19 Molecular Testing Kit 1.0 in the one-step real-time RT-qPCR test assay to qualitatively detect RNA from SARS-CoV-2 virus in the human respiratory specimen. It combines the reverse transcription technology and real-time PCR method to provide accurate detection of the SARS-CoV-2 coronavirus. The Star Array® magnetic beads RNA extraction instrument and kit can automatically extract and purify the RNA for 32 samples in 6 minutes. It is ideal for samples that contain high amount of PCR inhibitors and can't be processed by the direct amplification. The triplex fluorescence design of the kit simultaneously detects the N1 and Orf1ab genes of the virus and the human RNase P gene as an internal control to ensure the sample quality. This protocol uses the Star Array® XDiveTM Superfast Real-Time PCR instrument to perform 40-cycle PCR in 8 minutes, and can test up to 16 samples or controls in each run. The total protpcol time from sample collection to data interpretation is less than 15 minutes. | OnsiteGene Inc. | 1 | 2020 | yliu 2020. OnsiteGene 3 Protocol Nasal Extract. protocols.io https://dx.doi.org/10.17504/protocols.io.bkugkwtw | 2021-04-15 09:15:31 | |||
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NuFaction SARS-CoV2 Antigen Test Protocol Resource Report Resource Website |
Don Cooper, Timothy Wandell, ben | DOI:10.17504/protocols.io.bkv2kw8e | XPRIZE Rapid Covid Testing | Mobile Assay, NuFaction Inc., NuFaction Inc. | 1 | 2020 | Don Cooper, Timothy Wandell, ben 2020. NuFaction SARS-CoV2 Antigen Test Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bkv2kw8e | 2021-04-15 09:15:34 | ||||
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covid 19 indirect detection thru rise of 100nm filter fluid resistance Resource Report Resource Website |
Ladislav Nevery | DOI:10.17504/protocols.io.bmazk2f6 | Coronavirus Method Development Community, XPRIZE Rapid Covid Testing, Ladislav Nevery | By passing saliva sample thru 150nm filter to remove particles larger than avg 125nm sized covid 19 virus particles and concentrating resulting fluid to very small 1 mm2 area of 110nm filter. Pores in this small area should be clogged up fast raising filter air/fluid resistance. Due to high virus density 5.2 log10 ml saliva of infected person should clogg filter much faster than healthy person. Test is setup in such way that we let gravity pass 2l of fluid thru clogged filter and record rate of drops from its bottom as audio on mobile phone placed bellow glass cup. rate of drops thru clogged filter should be measurably slower than clean filter. | 4 | 2020 | Ladislav Nevery 2020. covid 19 indirect detection thru rise of 100nm filter fluid resistance. protocols.io https://dx.doi.org/10.17504/protocols.io.bmazk2f6 | 2021-04-15 09:15:33 | ||||
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V-CHEK Coronavirus (SARS-CoV-2) Antigen Detection Kit Resource Report Resource Website |
bill , covidtesting | DOI:10.17504/protocols.io.bkwukxew | XPRIZE Rapid Covid Testing | Reliable-LFC, LLC, XPRIZE Foundation | 1 | 2020 | bill , covidtesting 2020. V-CHEK Coronavirus (SARS-CoV-2) Antigen Detection Kit. protocols.io https://dx.doi.org/10.17504/protocols.io.bkwukxew | 2021-04-15 09:15:39 |
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