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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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PCR with Taq and ThermoPol® Buffer (M0267) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cifubm | New England Biolabs (NEB) | PCR Protocol for Taq DNA Polymerase with ThermoPol® Buffer (M0267) | New England Biolabs | https://www.neb.com/protocols/1/01/01/taq-dna-polymerase-with-thermopol-buffer-m0267 | 1 | 2015 | New England Biolabs 2015. PCR with Taq and ThermoPol® Buffer (M0267). protocols.io https://dx.doi.org/10.17504/protocols.io.cifubm | 2021-04-15 09:14:24 | ||
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NEB Monarch® RNA Cleanup Kit Resource Report Resource Website |
New England Biolabs | New England Biolabs (NEB) | New England Biolabs | https://neb.com/protocols/2018/06/28/monarch-rna-cleanup-kit-protocol | 1 | 2020 | New England Biolabs 2020. NEB Monarch® RNA Cleanup Kit. protocols.io https:// | 2021-04-15 09:14:22 | ||||
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E7805 NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® Protocol to use with Inputs ≥ 100 ng (Chapter 2) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.nwxdffn | New England Biolabs (NEB) | The NEBNext Ultra II FS DNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of DNA into high quality libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow also has minimal hands-on time.Note: The Ultra II FS Kit is not compatible with bisulfite conversion workflows. | New England Biolabs | 1 | 2021 | New England Biolabs 2021. E7805 NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® Protocol to use with Inputs ≥ 100 ng (Chapter 2). protocols.io https://dx.doi.org/10.17504/protocols.io.nwxdffn | 2021-04-15 09:14:26 | |||
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Enzymatic PCR Cleanup Protocol (NEB #M0525) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.7r4hm8w | New England Biolabs (NEB) | Rapid and irreversible heat inactivation eliminates unwanted activityImproved storage stability versus native enzymeFaster reaction setup (no supplemental additives like zinc required) and shorter incubation timeFlexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)Less enzyme required (high specific activity), resulting in a lower cost per reactionNo need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysisRecombinant for purity, consistency and value | New England Biolabs | https://neb.com/protocols/2019/06/04/enzymatic-pcr-cleanup-protocol-neb-m0525 | 1 | 2020 | New England Biolabs 2020. Enzymatic PCR Cleanup Protocol (NEB #M0525). protocols.io https://dx.doi.org/10.17504/protocols.io.7r4hm8w | 2021-04-15 09:14:30 | ||
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Dephosphorylation using CIP in Restriction Enzyme Reaction (M0290) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cdhs35 | New England Biolabs (NEB) | Protocol for Dephosphorylation of 5´-ends of DNA using CIP in Restriction Enzyme Reaction. Uses the Calf Intestinal Alkaline Phosphatase (CIP - M0290) | New England Biolabs | https://www.neb.com/protocols/1/01/01/protocol-for-dephosphorylating-with-cip | 1 | 2014 | New England Biolabs 2014. Dephosphorylation using CIP in Restriction Enzyme Reaction (M0290). protocols.io https://dx.doi.org/10.17504/protocols.io.cdhs35 | 2021-04-15 09:14:27 | ||
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Dephosphorylation using AnP (M0289) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cdns5d | New England Biolabs (NEB) | This is the protocol for dephosphorylation of 5'-ends of DNA using AnP (Antarctic Phosphatase - M0289). | New England Biolabs | https://www.neb.com/protocols/1/01/01/vector-dephosphorylation-protocol | 1 | 2014 | New England Biolabs 2014. Dephosphorylation using AnP (M0289). protocols.io https://dx.doi.org/10.17504/protocols.io.cdns5d | 2021-04-15 09:14:24 | ||
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Separating the Protein of Interest from MBP after TEV Protease Cleavage (NEB #E8201) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.bfebjjan | New England Biolabs (NEB) | Both the MBP-tag and TEV Protease are polyhistidine-tagged for easy removal from the TEV Protease reaction. Loading the digest onto NEBExpress Ni Resin (NEB #S1428) sequesters both the MBP-tag and TEV Protease, thereby isolating the protein of interest in the column flow through. The binding capacity of NEBExpress Ni Resin is ≥ 10 mg/ml. The binding capacity will vary depending on the target protein, binding conditions and the accessibility of the His-tag. It is recommended to estimate the amount of the His-tagged MBP released by TEV Protease cleavage by first analyzing a sample by SDS-PAGE and comparing its band size and intensity to that of the provided MBP6 protein control. | New England Biolabs | https://www.neb.com/protocols/2020/02/06/separating-the-protein-of-interest-from-mbp-after-tev-protease-cleavage-neb-e8201 | 1 | 2020 | New England Biolabs 2020. Separating the Protein of Interest from MBP after TEV Protease Cleavage (NEB #E8201). protocols.io https://dx.doi.org/10.17504/protocols.io.bfebjjan | 2021-04-15 09:14:29 | ||
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Protocol for T7 Exonuclease (NEB #M0263) Resource Report Resource Website |
New England Biolabs | New England Biolabs (NEB) | New England Biolabs | https://neb.com/protocols/2019/07/24/protocol-for-t7-exonuclease-m0263 | 1 | 2020 | New England Biolabs 2020. Protocol for T7 Exonuclease (NEB #M0263). protocols.io https:// | 2021-04-15 09:14:25 | ||||
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Non-radioactive phosphorylation with T4 PNK 3' phosphatase minus Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cp2vqd | New England Biolabs (NEB) | Protocol for non-radioactive phosphorylation with T4 PNK 3' phosphatase minus. | New England Biolabs | https://www.neb.com/protocols/1/01/01/non-radioactive-phosphorylation-with-t4-pnk-or-pnk3-phosphatase-minus | 1 | 2015 | New England Biolabs 2015. Non-radioactive phosphorylation with T4 PNK 3' phosphatase minus. protocols.io https://dx.doi.org/10.17504/protocols.io.cp2vqd | 2021-04-15 09:14:35 | ||
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NEBExpress Ni Resin Pressurized Column Typical Protocol (NEB #S1428) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.bfa4jigw | New England Biolabs (NEB) | NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.Purification of ≥10 mg His-tagged protein per 1 ml of resinIntended for use in gravity or pressure flow columns, and batch purificationsHigh specific binding of His-tagged proteins yielding purities of >95%Strong nickel ion binding provides excellent resistance to EDTA and reducing agents. Compatible with commercially available detergent-based cell lysis reagentsIsolation and purification of His-tagged fusion proteins under native or denaturing conditions | New England Biolabs | https://www.neb.com/protocols/2019/09/10/nebexpress-ni-resin-pressurized-column-protocol-neb-s1428 | 1 | 2020 | New England Biolabs 2020. NEBExpress Ni Resin Pressurized Column Typical Protocol (NEB #S1428). protocols.io https://dx.doi.org/10.17504/protocols.io.bfa4jigw | 2021-04-15 09:14:28 | ||
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E1202 Blunting Mixture Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.crqv5v | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/blunting-protocol-e1202 | 1 | 2015 | New England Biolabs 2015. E1202 Blunting Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.crqv5v | 2021-04-15 09:14:39 | |||
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Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.ejybcpw | New England Biolabs (NEB) | This is the quick version of the Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010). For the full protocol, please click here. | New England Biolabs | https://www.neb.com/protocols/2015/12/08/quick-protocol-for-monarch-plasmid-miniprep-kit-t1010 | 1 | 2016 | New England Biolabs 2016. Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010). protocols.io https://dx.doi.org/10.17504/protocols.io.ejybcpw | 2021-04-15 09:14:45 | ||
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NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®) E7760 Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.bsbrnam6 | New England Biolabs (NEB), Coronavirus Method Development Community | This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®), NEB #E7660S/L 24/96 reactions. | New England Biolabs | https://www.neb.com/-/media/nebus/files/manuals/manuale7660a.pdf?rev=4a88553b27064ccdbcdce76d05a2627c&hash=09A89395846012AA8A389C1DCFCE92F8 | 1 | 2021 | New England Biolabs 2021. NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®) E7760. protocols.io https://dx.doi.org/10.17504/protocols.io.bsbrnam6 | 2021-04-15 09:14:32 | ||
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Mixture for PCR Amplification (E7370) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cg7tzm | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2012/11/19/pcr-amplification-e7370 | 1 | 2015 | New England Biolabs 2015. Mixture for PCR Amplification (E7370). protocols.io https://dx.doi.org/10.17504/protocols.io.cg7tzm | 2021-04-15 09:14:35 | |||
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Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX Resource Report Resource Website |
New England BioLabs, Inc. | DOI:10.17504/protocols.io.frjbm4n | New England Biolabs (NEB) | Cas9 nuclease may be used in vivo to create targeted genome modifications. There are several ways in which to introduce Cas9-guide RNA complexes into cells. Here we present a method for the transfection of Cas9 RNP’s into HEK293 FT cells using Thermo Fisher Lipofectamine® RNAiMAX. This is a ‘reverse transfection’ method that uses a final concentration of 10 nM RNP per transfection in a 96-well culture plate. | , | https://www.neb.com/protocols/2016/07/26/transfection-of-cas9-rnp-ribonucleoprotein-into-adherent-cells-using-the-lipofectamine-rnaimax | 1 | 2016 | New England BioLabs, Inc. 2016. Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX. protocols.io https://dx.doi.org/10.17504/protocols.io.frjbm4n | 2021-04-15 09:14:39 | ||
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PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.7sehnbe | New England Biolabs (NEB) | Please note that protocols with Q5U Hot Start High-Fidelity DNA Polymerase may differ from protocols with other polymerases. Conditions recommended in the protocols should be used for optimal performance. | New England Biolabs | https://www.neb.com/protocols/2019/07/02/pcr-using-q5u-hot-start-high-fidelity-dna-polymerase-neb-m0515 | 1 | 2020 | New England Biolabs 2020. PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515). protocols.io https://dx.doi.org/10.17504/protocols.io.7sehnbe | 2021-04-15 09:14:42 | ||
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PCR Protocol for OneTaq® DNA Polymerase (M0480) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cq8vzv | New England Biolabs (NEB) | OneTaq DNA Polymerase allows for greater amplification sensitivity across a wide variety of amplicons regardless of GC content. | New England Biolabs | https://www.neb.com/protocols/2012/10/11/onetaqdnapolymerasem0480 | 1 | 2015 | New England Biolabs 2015. PCR Protocol for OneTaq® DNA Polymerase (M0480). protocols.io https://dx.doi.org/10.17504/protocols.io.cq8vzv | 2021-04-15 09:14:44 | ||
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Large (Klenow) Fragment Blunting (M0210) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cpgvjv | New England Biolabs (NEB) | Protocol for blunting ends by 3' overhang removal and fill-in of 3' recessed (5' overhang) ends using DNA Polymerase I, Large (Klenow) Fragment (M0210) | NEB | https://www.neb.com/protocols/2014/01/13/protocol-for-blunting-ends-by-3-overhang-removal-and-fill-in-of-3-recessed-5-overhang-ends-using | 1 | 2015 | New England Biolabs 2015. Large (Klenow) Fragment Blunting (M0210). protocols.io https://dx.doi.org/10.17504/protocols.io.cpgvjv | 2021-04-15 09:15:06 | ||
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Restriction Digest Reaction Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.iszcef6 | New England Biolabs (NEB) | NEB, Ipswich, MA | https://www.neb.com/protocols/2014/05/07/double-digest-protocol-with-standard-restriction-enzymes | 2 | 2018 | New England Biolabs 2018. Restriction Digest Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.iszcef6 | 2021-04-15 09:15:06 | |||
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Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.bp9dmr26 | New England Biolabs (NEB) | Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030) | New England Biolabs | https://www.neb.com/protocols/2017/04/25/quick-protocol-for-oligonucleotide-cleanup-using-the-monarch-pcr-dna-cleanup-kit-5-g-neb-t1030 | 2 | 2020 | New England Biolabs 2020. Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030). protocols.io https://dx.doi.org/10.17504/protocols.io.bp9dmr26 | 2021-04-15 09:14:47 |
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