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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
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High Efficiency Transformation Protocol (C2987I) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.chht35 | New England Biolabs (NEB) | This is the correct protocol if you are using the C2987I cells. If you are using the C2987H cells, please refer to this protocol. | New England Biolabs | https://www.neb.com/protocols/1/01/01/high-efficiency-transformation-protocol-c2987 | 1 | 2014 | New England Biolabs 2014. High Efficiency Transformation Protocol (C2987I). protocols.io dx.doi.org/10.17504/protocols.io.chht35 | 2021-03-29 03:10:41 | ||
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PCR Protocol for Phusion® High-Fidelity DNA Polymerase (M0530) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.irrcd56 | New England Biolabs (NEB) | This is the PCR protocol for Phusion® High-Fidelity DNA Polymerase (M0530) | New England Biolabs | https://www.neb.com/protocols/1/01/01/pcr-protocol-m0530 | 2 | 2017 | New England Biolabs 2017. PCR Protocol for Phusion® High-Fidelity DNA Polymerase (M0530). protocols.io dx.doi.org/10.17504/protocols.io.irrcd56 | 2021-03-29 03:10:50 | ||
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Transformation Protocol Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cpxvpm | New England Biolabs (NEB) | Quick Ligation products may be transformed by many different methods. The following protocol is recommended by New England Biolabs. | New England Biolabs | https://www.neb.com/protocols/2012/05/21/transformation-protocol | 1 | 2015 | New England Biolabs 2015. Transformation Protocol. protocols.io dx.doi.org/10.17504/protocols.io.cpxvpm | 2021-03-29 03:10:51 | ||
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NEBExpress Ni Resin Batch Binding Typical Protocol (NEB #S1428) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.bfd5ji86 | New England Biolabs (NEB) | NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.Purification of ≥10 mg His-tagged protein per 1 ml of resinIntended for use in gravity or pressure flow columns, and batch purificationsHigh specific binding of His-tagged proteins yielding purities of >95%Strong nickel ion binding provides excellent resistance to EDTA and reducing agents. Compatible with commercially available detergent-based cell lysis reagentsIsolation and purification of His-tagged fusion proteins under native or denaturing conditions | New England Biolabs | https://www.neb.com/protocols/2019/09/10/nebexpress-ni-resin-batch-binding-protocol | 1 | 2020 | New England Biolabs 2020. NEBExpress Ni Resin Batch Binding Typical Protocol (NEB #S1428). protocols.io dx.doi.org/10.17504/protocols.io.bfd5ji86 | 2021-03-29 03:10:49 | ||
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PNGase F Non-Denaturing Mixture Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cqjvum | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2014/07/31/pngase-f-protocol | 1 | 2015 | New England Biolabs 2015. PNGase F Non-Denaturing Mixture. protocols.io dx.doi.org/10.17504/protocols.io.cqjvum | 2021-03-29 03:11:13 | |||
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Electroporation Protocol (C2986) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.crgv3v | New England Biolabs (NEB) | This electroporation protocol is for use with the NEB Turbo Electrocompetent E. coli cells (C2986). These cells are suitable for high efficiency electroporation and rapid colony growth; they are ideal for DNA library constructions and all cloning purposes. | New England Biolabs | https://www.neb.com/protocols/1/01/01/electroporation-protocol-c2986 | 1 | 2015 | New England Biolabs 2015. Electroporation Protocol (C2986). protocols.io dx.doi.org/10.17504/protocols.io.crgv3v | 2021-03-29 03:09:32 | ||
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E1202 Ligation Mixture Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cp6vrd | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2013/12/27/ligation-protocol-e1202 | 1 | 2015 | New England Biolabs 2015. E1202 Ligation Mixture. protocols.io dx.doi.org/10.17504/protocols.io.cp6vrd | 2021-03-29 03:09:46 | |||
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Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.nytdfwn | New England Biolabs (NEB) | This is the quick version of the Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010). For the full protocol, please click here. | New England Biolabs | https://www.neb.com/protocols/2015/12/08/quick-protocol-for-monarch-plasmid-miniprep-kit-t1010 | 2 | 2018 | New England Biolabs 2018. Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010). protocols.io dx.doi.org/10.17504/protocols.io.nytdfwn | 2021-03-29 03:09:51 | ||
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NEB 3X SDS Sample Loading Buffer Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.crcv2v | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/immunoprecipitation-using-protein-ag-magnetic-beads | 1 | 2015 | New England Biolabs 2015. NEB 3X SDS Sample Loading Buffer. protocols.io dx.doi.org/10.17504/protocols.io.crcv2v | 2021-03-29 03:09:51 | |||
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BioBrick E0546 Destination Reaction Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cpmvk5 | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/digestion-protocol-e0546 | 1 | 2015 | New England Biolabs 2015. BioBrick E0546 Destination Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cpmvk5 | 2021-03-29 03:09:50 | |||
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Restriction Digest Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.isycefw | New England Biolabs (NEB) | The following is a "typical" restriction endonuclease reaction. Please see the "guidelines" tab below for the NEB tips on optimizing restriction digests. | NEB, Ipswich, MA | https://www.neb.com/protocols/2012/12/07/optimizing-restriction-endonuclease-reactions | 2 | 2018 | New England Biolabs 2018. Restriction Digest. protocols.io dx.doi.org/10.17504/protocols.io.isycefw | 2021-03-29 03:09:55 | ||
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Quick Protocol for Monarch® DNA Gel Extraction Kit (NEB #T1020) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.ejwbcpe | New England Biolabs (NEB) | This is the quick version of the Monarch® DNA Gel Extraction Kit Protocol (NEB #T1020). For the full protocol, please click here. | New England Biolabs | https://www.neb.com/protocols/2015/12/08/quick-protocol-for-monarch-dna-gel-extraction-kit-t1020 | 1 | 2016 | New England Biolabs 2016. Quick Protocol for Monarch® DNA Gel Extraction Kit (NEB #T1020). protocols.io dx.doi.org/10.17504/protocols.io.ejwbcpe | 2021-03-29 03:10:06 | ||
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First Strand cDNA Synthesis (M0368) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.chtt6m | New England Biolabs (NEB) | This protocol is for the "First Strand cDNA Synthesis Kit using ProtoScript II Reverse Transcriptase (M0368)". | New England Biolabs | https://www.neb.com/protocols/2012/10/03/first-strand-cdna-synthesis-kit-using-protoscript-ii-reverse-transcriptase-m0368 | 1 | 2014 | New England Biolabs 2014. First Strand cDNA Synthesis (M0368). protocols.io dx.doi.org/10.17504/protocols.io.chtt6m | 2021-03-29 03:10:18 | ||
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NEBExpress MBP Fusion and Purification System (NEB #E8201) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.bfayjifw | New England Biolabs (NEB) | The NEBExpress MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein inE. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.ReliableE. coli expression: substantial yields (up to 100 mg/L)Fusion to MBP has been shown to enhance the solubility of proteins expressed inE. coli(1)Two-step purification: amylose elution followed by TEV Protease cleavage and Ni resin isolation results in a highly pure tag-free target proteinGentle elution with maltose; no detergents or harsh denaturants required | New England Biolabs | https://www.neb.com/protocols/2020/02/05/nebexpress-mbp-fusion-and-purification-system-quick-start-protocol-neb-e8201 | 1 | 2020 | New England Biolabs 2020. NEBExpress MBP Fusion and Purification System (NEB #E8201). protocols.io dx.doi.org/10.17504/protocols.io.bfayjifw | 2021-03-29 03:10:32 | ||
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PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (M0493) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cjtunm | New England Biolabs (NEB) | This protocols is for PCR using Q5® High-Fidelity DNA Polymerase (M0491) | New England Biolabs | https://www.neb.com/protocols/2012/08/30/pcr-using-q5-hot-start-high-fidelity-dna-polymerase-m0493 | 1 | 2015 | New England Biolabs 2015. PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (M0493). protocols.io dx.doi.org/10.17504/protocols.io.cjtunm | 2021-03-29 03:10:27 | ||
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Quick Ligation Protocol (M2200) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cdgs3v | New England Biolabs (NEB) | This protocols is to be performed with the Quick Ligation Reaction Buffer. Please see the NEB website for more information. | New England Biolabs | https://www.neb.com/protocols/1/01/01/quick-ligation-protocol | 1 | 2014 | New England Biolabs 2014. Quick Ligation Protocol (M2200). protocols.io dx.doi.org/10.17504/protocols.io.cdgs3v | 2021-03-29 03:08:50 | ||
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NEBExpress Ni Resin Gravity Flow Typical Protocol (NEB #S1428) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.bfd7ji9n | New England Biolabs (NEB) | NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.Purification of ≥10 mg His-tagged protein per 1 ml of resinIntended for use in gravity or pressure flow columns, and batch purificationsHigh specific binding of His-tagged proteins yielding purities of >95%Strong nickel ion binding provides excellent resistance to EDTA and reducing agents. Compatible with commercially available detergent-based cell lysis reagentsIsolation and purification of His-tagged fusion proteins under native or denaturing conditions | New England Biolabs | https://www.neb.com/protocols/2019/09/10/nebexpress-ni-resin-gravity-flow-typical-protocol | 1 | 2020 | New England Biolabs 2020. NEBExpress Ni Resin Gravity Flow Typical Protocol (NEB #S1428). protocols.io dx.doi.org/10.17504/protocols.io.bfd7ji9n | 2021-03-29 03:08:50 | ||
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E7805 NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® Protocol for use with Inputs ≤ 100 ng Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.k8tczwn | New England Biolabs (NEB) | The NEBNext Ultra II FS DNA Module contains the enzymes and buffers required to convert a broad range of input amounts of intact DNA into fragmented DNA with 5´ phosphorylated 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595) and with the NEBNext Ultra II Q5 Master Mix (NEB #M0544) if amplification is required. The fast, user-friendly workflow has minimal hands on time. | New England Biolabs | https://www.neb.com/-/media/nebus/files/manuals/manuale7810.pdf?rev=70d2cafe09b946ceaadff2996ed02c5b&hash=732A8D3E3E8F777DC946F10C3CBC7629 | 1 | 2020 | New England Biolabs 2020. E7805 NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® Protocol for use with Inputs ≤ 100 ng. protocols.io dx.doi.org/10.17504/protocols.io.k8tczwn | 2021-03-29 03:08:57 | ||
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NEBNext End Prep Mixture Resource Report Resource Website |
Isabel Gautreau | 10.17504/protocols.io.cg4tyv | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2012/11/15/nebnext-end-prep-e7370 | 1 | 2015 | Isabel Gautreau 2015. NEBNext End Prep Mixture. protocols.io dx.doi.org/10.17504/protocols.io.cg4tyv | 2021-03-29 03:08:30 | |||
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A-Tailing with Taq Mixture Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.crxv7m | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2013/11/01/a-tailing-with-taq-polymerase | 1 | 2015 | New England Biolabs 2015. A-Tailing with Taq Mixture. protocols.io dx.doi.org/10.17504/protocols.io.crxv7m | 2021-03-29 03:08:32 |
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