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Authors: Stephen M. Griffey
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:
Gross necropsy with documentation of changes present. Collection and histologic processing (paraffin blocks/H&E slides) and interpretation of kidneys, liver, spleen, pancreas, heart, lungs, esophagus, trachea, thymus, mesenteric lymph nodes, GI Tract, cerebrum, cerebellum, urinary bladder, and reproductive tract and additional tissues with gross changes. This includes photodocumentation of significant findings. This assay can be customized to include other target organs based on findings from other MMPC assays.
This procedure is to be followed by trained Comparative Pathology Laboratory (CPL) staff responsible for performing necrospy diagnostics procedures at CPL.
Proper citation: Stephen M. Griffey 2019. UC Davis - Mouse Gross Necropsy with Histology. protocols.io dx.doi.org/10.17504/protocols.io.yusfwwe Copy
Authors: Peter Havel
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:
Hydrogen peroxide (H2O2) is a ubiquitous, toxic, metabolic by-product of aerobic respiration, oxidative stress, and oxidative injury. Cayman's Hydrogen Peroxide Assay Kit utilizes the well established xylenol orange detection method of quantifying the oxidation of ferrous ions (Fe2+) to ferric ions (Fe3+) by hydrogen peroxide. A unique feature of Cayman's assay is the inclusion of catalase as an H2O2 scavenger for the purpose of confirming the specificity of the reaction for H2O2. The sensitivity and the specificity of the assay make it well suited to accurately measure urinary levels H2O2 in a 96 well plate format. Each kit contains hydrogen peroxide, reagent 1, reagent 2, catalase, a 96 well plate, plate cover, and complete instructions.
Proper citation: Peter Havel 2019. UC Davis - Hydrogen Peroxide. protocols.io dx.doi.org/10.17504/protocols.io.ypqfvmw Copy
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