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Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the correct protocol if you are using the C2987I cells. If you are using the C2987H cells, please refer to this protocol.
Proper citation: New England Biolabs 2014. High Efficiency Transformation Protocol (C2987I). protocols.io dx.doi.org/10.17504/protocols.io.chht35 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the PCR protocol for Phusion® High-Fidelity DNA Polymerase (M0530)
Proper citation: New England Biolabs 2017. PCR Protocol for Phusion® High-Fidelity DNA Polymerase (M0530). protocols.io dx.doi.org/10.17504/protocols.io.irrcd56 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick Ligation products may be transformed by many different methods. The following protocol is recommended by New England Biolabs.
Proper citation: New England Biolabs 2015. Transformation Protocol. protocols.io dx.doi.org/10.17504/protocols.io.cpxvpm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.Purification of ≥10 mg His-tagged protein per 1 ml of resinIntended for use in gravity or pressure flow columns, and batch purificationsHigh specific binding of His-tagged proteins yielding purities of >95%Strong nickel ion binding provides excellent resistance to EDTA and reducing agents. Compatible with commercially available detergent-based cell lysis reagentsIsolation and purification of His-tagged fusion proteins under native or denaturing conditions
Proper citation: New England Biolabs 2020. NEBExpress Ni Resin Batch Binding Typical Protocol (NEB #S1428). protocols.io dx.doi.org/10.17504/protocols.io.bfd5ji86 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. PNGase F Non-Denaturing Mixture. protocols.io dx.doi.org/10.17504/protocols.io.cqjvum Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This electroporation protocol is for use with the NEB Turbo Electrocompetent E. coli cells (C2986). These cells are suitable for high efficiency electroporation and rapid colony growth; they are ideal for DNA library constructions and all cloning purposes.
Proper citation: New England Biolabs 2015. Electroporation Protocol (C2986). protocols.io dx.doi.org/10.17504/protocols.io.crgv3v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. E1202 Ligation Mixture. protocols.io dx.doi.org/10.17504/protocols.io.cp6vrd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. NEB 3X SDS Sample Loading Buffer. protocols.io dx.doi.org/10.17504/protocols.io.crcv2v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. BioBrick E0546 Destination Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cpmvk5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocol is for the "First Strand cDNA Synthesis Kit using ProtoScript II Reverse Transcriptase (M0368)".
Proper citation: New England Biolabs 2014. First Strand cDNA Synthesis (M0368). protocols.io dx.doi.org/10.17504/protocols.io.chtt6m Copy
Authors: New England Biolabs
Group: Grupo de Estudos de Leveduras Cervejeiras
Proper citation: New England Biolabs 2018. SOC Media. protocols.io dx.doi.org/10.17504/protocols.io.pngdmbw Copy
Authors: New England Biolabs
Group: Coronavirus Method Development Community
Summary: This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®), NEB #E7660S/L 24/96 reactions.
Proper citation: New England Biolabs 2021. NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®) E7660. protocols.io dx.doi.org/10.17504/protocols.io.btcenite Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBExpress MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein inE. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.ReliableE. coli expression: substantial yields (up to 100 mg/L)Fusion to MBP has been shown to enhance the solubility of proteins expressed inE. coli(1)Two-step purification: amylose elution followed by TEV Protease cleavage and Ni resin isolation results in a highly pure tag-free target proteinGentle elution with maltose; no detergents or harsh denaturants required
Proper citation: New England Biolabs 2020. NEBExpress MBP Fusion and Purification System (NEB #E8201). protocols.io dx.doi.org/10.17504/protocols.io.bfayjifw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocols is for PCR using Q5® High-Fidelity DNA Polymerase (M0491)
Proper citation: New England Biolabs 2015. PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (M0493). protocols.io dx.doi.org/10.17504/protocols.io.cjtunm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocols is to be performed with the Quick Ligation Reaction Buffer. Please see the NEB website for more information.
Proper citation: New England Biolabs 2014. Quick Ligation Protocol (M2200). protocols.io dx.doi.org/10.17504/protocols.io.cdgs3v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.Purification of ≥10 mg His-tagged protein per 1 ml of resinIntended for use in gravity or pressure flow columns, and batch purificationsHigh specific binding of His-tagged proteins yielding purities of >95%Strong nickel ion binding provides excellent resistance to EDTA and reducing agents. Compatible with commercially available detergent-based cell lysis reagentsIsolation and purification of His-tagged fusion proteins under native or denaturing conditions
Proper citation: New England Biolabs 2020. NEBExpress Ni Resin Gravity Flow Typical Protocol (NEB #S1428). protocols.io dx.doi.org/10.17504/protocols.io.bfd7ji9n Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II FS DNA Module contains the enzymes and buffers required to convert a broad range of input amounts of intact DNA into fragmented DNA with 5´ phosphorylated 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595) and with the NEBNext Ultra II Q5 Master Mix (NEB #M0544) if amplification is required. The fast, user-friendly workflow has minimal hands on time.
Proper citation: New England Biolabs 2020. E7805 NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® Protocol for use with Inputs ≤ 100 ng. protocols.io dx.doi.org/10.17504/protocols.io.k8tczwn Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. A-Tailing with Taq Mixture. protocols.io dx.doi.org/10.17504/protocols.io.crxv7m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. PNGase F Mixture 1. protocols.io dx.doi.org/10.17504/protocols.io.cqgvtv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Protocol for blunting ends by 3' overhang removal and fill-in of 3' recessed (5' overhang) ends using T4 DNA Polymerase.
Proper citation: New England Biolabs 2015. Blunting Protocol (M0203). protocols.io dx.doi.org/10.17504/protocols.io.cgwtxd Copy
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