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Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:
This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity.
Cytokines Panel I include IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KC (IL-8 homologue), IL-9, IL-10 (interleukin-10), IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, TNFα (tumor necrosis factor alpha), IFNγ (interferon gamma), IP-10 (interferon gamma-induced protein 10; CXCL-10), Eotaxin (CCL-11), G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte macrophage colony stimulating factor), LIF (leukemia inhibitory factor), LIX (LPS- induced CXC chemokine), MCP-1 (monocyte chemotactic protein-1; CCL-2), M-CSF (macrophage colony stimulating factor), MIG (monokine induced by gamma interferon; CXCL- 9), MIP-1α (macrophage inflammatory protein alpha; CCL-3), MIP-1β (macrophage inflammatory protein beta; CCL-4), and MIP-2/RANTES (macrophage inflammatory protein 2- alpha; CXCL-2).
A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay.
Proper citation: Jason Kim 2019. U Mass - Electrolytes. protocols.io dx.doi.org/10.17504/protocols.io.xwafpae Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Glucose tolerance test measures systemic clearance of glucose following an intraperitoneal bolus injection of 20% dextrose. This experiment measures insulin sensitivity in awake mice assuming that there are no alterations in the animal’s pancreatic -cell function and insulin secretion. Insulin sensitivity is altered in obese mice.
Proper citation: Jason Kim 2019. U Mass - Glucose Tolerance Test. protocols.io dx.doi.org/10.17504/protocols.io.xxafpie Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum Urea/BUN levels are affected by alterations in systemic protein and nitrogen metabolism. Serum Urea/BUN levels are altered in kidney failure and renal complications of diabetes.
Proper citation: Jason Kim 2019. U Mass - Urea/BUN. protocols.io dx.doi.org/10.17504/protocols.io.x5qfq5w Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:In-cage free running wheel is used to voluntarily induce exercise in awake mice. Exercise affects insulin sensitivity and glucose metabolism.
Proper citation: Jason Kim 2019. U Mass - Exercise model. protocols.io dx.doi.org/10.17504/protocols.io.xwyfpfw Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity. Cytokines Panel I include IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KC (IL-8 homologue), IL-9, IL-10 (interleukin-10), IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, TNFα (tumor necrosis factor alpha), IFNγ (interferon gamma), IP-10 (interferon gamma-induced protein 10; CXCL-10), Eotaxin (CCL-11), G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte macrophage colony stimulating factor), LIF (leukemia inhibitory factor), LIX (LPSinduced CXC chemokine), MCP-1 (monocyte chemotactic protein-1; CCL-2), M-CSF (macrophage colony stimulating factor), MIG (monokine induced by gamma interferon; CXCL9), MIP-1α (macrophage inflammatory protein alpha; CCL-3), MIP-1β (macrophage inflammatory protein beta; CCL-4), and MIP-2/RANTES (macrophage inflammatory protein 2alpha; CXCL-2). A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay.
Proper citation: Jason Kim 2019. U Mass - Hemoglobin A1c. protocols.io dx.doi.org/10.17504/protocols.io.xy3fpyn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
Lipid metabolism is estimated by measuring systemic clearance of [1-14C] palmitate following a bolus injection in awake mice. Lipid metabolism is altered in obese mice.
Proper citation: Jason Kim 2019. U Mass - Lipid metabolism. protocols.io dx.doi.org/10.17504/protocols.io.x3gfqjw Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:
Insulin clearance test measure systemic clearance of insulin following a bolus injection. Liver accounts for the majority of systemic insulin clearance following secretion from pancreatic β- cells into portal circulation. Hepatic clearance of insulin may be affected by obesity and in other mouse models of altered metabolism. Alterations in insulin clearance may also affect glucose and lipid metabolism.
Proper citation: Jason Kim 2019. U Mass - Insulin clearance. protocols.io dx.doi.org/10.17504/protocols.io.x2wfqfe Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:This experiment provides the quantification of multiple hormones using multiplexed-Luminex technology based on beads containing specific antibodies. Sample matrices include plasma, serum, tissue culture supernatants, and tissue or cell lysates. Serum C-peptide levels reflect insulin secretion and pancreatic -cell function. Serum C-peptide levels are altered in obesity, insulin resistance, and type 1 and type 2 diabetes.
Proper citation: Jason Kim 2019. U Mass - C-Peptide. protocols.io dx.doi.org/10.17504/protocols.io.xttfnnn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of creatinine reflect systemic protein metabolism.
Proper citation: Jason Kim 2019. U Mass - Creatinine. protocols.io dx.doi.org/10.17504/protocols.io.xvjfn4n Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment measures blood or plasma glucose concentration using Analox GM9 Glucose Analyzer or GM7 Micro-Stat Rapid Multi-assay Analyzer. The measurement is based on the rate of oxygen uptake in the reaction between sample glucose and glucose oxidase. Plasma glucose levels are elevated (i.e., hyperglycemia) in obesity, insulin resistance, and type 2 diabetes.
Proper citation: Jason Kim 2019. U Mass - Glucose. protocols.io dx.doi.org/10.17504/protocols.io.xw5fpg6 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:Hepatic gluconeogensis is estimated using pyruvate tolerance test that measures systemic elevation of glucose partly derived from pyruvate and hepatic gluconeogensis following an intraperitoneal bolus injection of pyruvate in awake mice. Hepatic gluconeogenesis is affected by obesity and regulates glucose homeostasis.
Proper citation: Jason Kim 2019. U Mass - Hepatic gluconeogenesis. protocols.io dx.doi.org/10.17504/protocols.io.xy7fpzn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity. Cytokines Panel I include IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KC (IL-8 homologue), IL-9, IL-10 (interleukin-10), IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, TNFα (tumor necrosis factor alpha), IFNγ (interferon gamma), IP-10 (interferon gamma-induced protein 10; CXCL-10), Eotaxin (CCL-11), G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte macrophage colony stimulating factor), LIF (leukemia inhibitory factor), LIX (LPSinduced CXC chemokine), MCP-1 (monocyte chemotactic protein-1; CCL-2), M-CSF (macrophage colony stimulating factor), MIG (monokine induced by gamma interferon; CXCL9), MIP-1α (macrophage inflammatory protein alpha; CCL-3), MIP-1β (macrophage inflammatory protein beta; CCL-4), and MIP-2/RANTES (macrophage inflammatory protein 2alpha; CXCL-2). A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay.
Proper citation: Jason Kim 2019. U Mass - Gamma-glutamyl Transferase. protocols.io dx.doi.org/10.17504/protocols.io.xwzfpf6 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:Hyperglycemic clamp measures insulin secretion and pancreatic -cell function in awake mice. Islet function is affected by obesity and insulin resistance, and abnormal islet function predisposes to the development of type 2 diabetes.
Proper citation: Jason Kim 2019. U Mass - Hyperglycemic clamp. protocols.io dx.doi.org/10.17504/protocols.io.xzafp2e Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
Insulin tolerance test measures systemic clearance of glucose following an intraperitoneal bolus injection of a physiological dose of insulin. This experiment measures insulin sensitivity in awake mice assuming that there are no alterations in the animal’s counterregulatory response. Insulin sensitivity is altered in obese mice.
Proper citation: Jason Kim 2019. U Mass - Insulin tolerance test. protocols.io dx.doi.org/10.17504/protocols.io.x2xfqfn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:The insulin enzyme-linked immunosorbent assay (ELISA) is a standard immunoassay for the detection of insulin levels in serum samples. This experiment uses a 96-well plate coated with anti-insulin antibodies and developed with a secondary anti-insulin antibody linked to horseradish peroxidase. The ELISA is developed with 3,3',5,5'-Tetramethylbenzidine (TMB), a chromogenic substrate and read on an automated plate spectrophotometer at 450 nm. Insulin ELISA may be processed with normal or ultra-sensitive assay kit depending on the expected range of sample concentrations. Serum insulin levels are affected by pancreatic β-cell function and insulin sensitivity. Serum insulin levels are elevated in obesity and insulin resistance.
Proper citation: Jason Kim 2019. U Mass - Insulin. protocols.io dx.doi.org/10.17504/protocols.io.xznfp5e Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum uric acid levels are affected by alterations in systemic protein and nitrogen metabolism. Serum uric acid levels are altered in kidney failure and renal complications of diabetes.
Proper citation: Jason Kim 2019. U Mass - Uric Acid. protocols.io dx.doi.org/10.17504/protocols.io.x5sfq6e Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Albumin is a major circulating protein in body.
Proper citation: Jason Kim 2019. U Mass - Albumin. protocols.io dx.doi.org/10.17504/protocols.io.xprfmm6 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: A subcutaneous or intraperitoneal implantation of Alzet osmotic pump is used to chronically administer selected drug in mice. Chronic drug delivery may be used to examine intermediate to long-term effects of selected drug on obesity, insulin resistance, and metabolism.
Proper citation: Jason Kim 2019. U Mass - Chronic drug delivery. protocols.io dx.doi.org/10.17504/protocols.io.xuefnte Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of aspartate transferase reflect liver function.
Proper citation: Jason Kim 2019. U Mass - Aspartate Transferase. protocols.io dx.doi.org/10.17504/protocols.io.xr5fm86 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
This experiment provides the quantification of multiple hormones using multiplexed-Luminex technology based on beads containing specific antibodies. Sample matrices include plasma, serum, tissue culture supernatants, and tissue or cell lysates. Leptin is an adipocyte-derived hormone (adipokine) that regulates feeding behavior (hunger/satiety). Serum leptin levels are altered in obesity, insulin resistance, and type 2 diabetes.
Proper citation: Jason Kim 2019. U Mass - Leptin. protocols.io dx.doi.org/10.17504/protocols.io.x23fqgn Copy
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