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Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:
This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity.
Cytokines Panel I include IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KC (IL-8 homologue), IL-9, IL-10 (interleukin-10), IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, TNFα (tumor necrosis factor alpha), IFNγ (interferon gamma), IP-10 (interferon gamma-induced protein 10; CXCL-10), Eotaxin (CCL-11), G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte macrophage colony stimulating factor), LIF (leukemia inhibitory factor), LIX (LPS- induced CXC chemokine), MCP-1 (monocyte chemotactic protein-1; CCL-2), M-CSF (macrophage colony stimulating factor), MIG (monokine induced by gamma interferon; CXCL- 9), MIP-1α (macrophage inflammatory protein alpha; CCL-3), MIP-1 (macrophage inflammatory protein beta; CCL-4), and MIP-2/RANTES (macrophage inflammatory protein 2- alpha; CXCL-2).
A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay.
Proper citation: Jason Kim 2019. U Mass - Cytokines Panel I - multiplex. protocols.io https://dx.doi.org/10.17504/protocols.io.xvnfn5e Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Albumin is a major circulating protein in body.
Proper citation: Jason Kim 2019. U Mass - Albumin. protocols.io https://dx.doi.org/10.17504/protocols.io.xprfmm6 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of alkaline phosphatase reflect liver function.
Proper citation: Jason Kim 2019. U Mass - Alkaline Phosphatase. protocols.io https://dx.doi.org/10.17504/protocols.io.xptfmnn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
The EchoMRI 3-in-1 uses ¹H- magnetic resonance spectroscopy to noninvasively measure fat mass, lean mass, and water mass in individual organs. Fat, lean, and water mass composition in specific organs is altered in obesity.
Proper citation: Jason Kim 2019. U Mass - Body composition (organs). protocols.io https://dx.doi.org/10.17504/protocols.io.xsnfnde Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer.
Proper citation: Jason Kim 2019. U Mass - Ammonia. protocols.io https://dx.doi.org/10.17504/protocols.io.xq3fmyn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment provides the quantification of multiple hormones using multiplexed-Luminex technology based on beads containing specific antibodies. Sample matrices include plasma, serum, tissue culture supernatants, and tissue or cell lysates. Resistin is an adipocyte-derived hormone (adipokine) that regulates insulin sensitivity and glucose metabolism. Serum resistin levels are altered in obesity, insulin resistance, and type 2 diabetes.
Proper citation: Jason Kim 2019. U Mass - Resistin. protocols.io https://dx.doi.org/10.17504/protocols.io.x4kfquw Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of total cholesterol reflect cholesterol metabolism and are associated with cardiovascular disease.
Proper citation: Jason Kim 2019. U Mass - Cholesterol (Total). protocols.io https://dx.doi.org/10.17504/protocols.io.xucfnsw Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of aspartate transferase reflect liver function.
Proper citation: Jason Kim 2019. U Mass - Aspartate Transferase. protocols.io https://dx.doi.org/10.17504/protocols.io.xr5fm86 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of LDL-cholesterol reflect cholesterol metabolism and are associated with cardiovascular disease.
Proper citation: Jason Kim 2019. U Mass - Cholesterol (LDL). protocols.io https://dx.doi.org/10.17504/protocols.io.xt9fnr6 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:Hepatic gluconeogensis is estimated using pyruvate tolerance test that measures systemic elevation of glucose partly derived from pyruvate and hepatic gluconeogensis following an intraperitoneal bolus injection of pyruvate in awake mice. Hepatic gluconeogenesis is affected by obesity and regulates glucose homeostasis.
Proper citation: Jason Kim 2019. U Mass - Hepatic gluconeogenesis. protocols.io https://dx.doi.org/10.17504/protocols.io.xy7fpzn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity. Cytokines Panel I include IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KC (IL-8 homologue), IL-9, IL-10 (interleukin-10), IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, TNFα (tumor necrosis factor alpha), IFNγ (interferon gamma), IP-10 (interferon gamma-induced protein 10; CXCL-10), Eotaxin (CCL-11), G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte macrophage colony stimulating factor), LIF (leukemia inhibitory factor), LIX (LPSinduced CXC chemokine), MCP-1 (monocyte chemotactic protein-1; CCL-2), M-CSF (macrophage colony stimulating factor), MIG (monokine induced by gamma interferon; CXCL9), MIP-1α (macrophage inflammatory protein alpha; CCL-3), MIP-1β (macrophage inflammatory protein beta; CCL-4), and MIP-2/RANTES (macrophage inflammatory protein 2alpha; CXCL-2). A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay.
Proper citation: Jason Kim 2019. U Mass - Gamma-glutamyl Transferase. protocols.io https://dx.doi.org/10.17504/protocols.io.xwzfpf6 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Streptozotocin can selectively destroy the pancreatic β-cells with rapid and irreversible necrosis and can be used to generate a chronic model of hyperglycemia and type 1 diabetes.
Proper citation: Jason Kim 2019. U Mass - STZ-induced type 1 diabetes model. protocols.io https://dx.doi.org/10.17504/protocols.io.xh8fj9w Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity. Cytokines Panel I include IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KC (IL-8 homologue), IL-9, IL-10 (interleukin-10), IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, TNFα (tumor necrosis factor alpha), IFNγ (interferon gamma), IP-10 (interferon gamma-induced protein 10; CXCL-10), Eotaxin (CCL-11), G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte macrophage colony stimulating factor), LIF (leukemia inhibitory factor), LIX (LPSinduced CXC chemokine), MCP-1 (monocyte chemotactic protein-1; CCL-2), M-CSF (macrophage colony stimulating factor), MIG (monokine induced by gamma interferon; CXCL9), MIP-1α (macrophage inflammatory protein alpha; CCL-3), MIP-1β (macrophage inflammatory protein beta; CCL-4), and MIP-2/RANTES (macrophage inflammatory protein 2alpha; CXCL-2). A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay.
Proper citation: Jason Kim 2019. U Mass - Hemoglobin A1c. protocols.io https://dx.doi.org/10.17504/protocols.io.xy3fpyn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:In-cage free running wheel is used to voluntarily induce exercise in awake mice. Exercise affects insulin sensitivity and glucose metabolism.
Proper citation: Jason Kim 2019. U Mass - Exercise model. protocols.io https://dx.doi.org/10.17504/protocols.io.xwyfpfw Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: The adiponectin enzyme-linked immunosorbent assay (ELISA) is a standard immunoassay for the detection of total adiponectin levels in serum, adipocyte extracts, and cell culture media samples. This experiment uses a 96-well plate coated with anti-adiponectin antibodies and developed with a secondary anti-adiponectin antibody linked to horseradish peroxidase. The ELISA is developed with 3,3',5,5'-Tetramethylbenzidine (TMB), a chromogenic substrate and read on an automated plate spectrophotometer at 450 nm. Adiponectin is an adipocyte-derived hormone (adipokine) that regulates insulin sensitivity and glucose metabolism.
Proper citation: Jason Kim 2019. U Mass - Adiponectin. protocols.io https://dx.doi.org/10.17504/protocols.io.xm9fk96 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of creatinine reflect systemic protein metabolism.
Proper citation: Jason Kim 2019. U Mass - Creatinine. protocols.io https://dx.doi.org/10.17504/protocols.io.xvjfn4n Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum Urea/BUN levels are affected by alterations in systemic protein and nitrogen metabolism. Serum Urea/BUN levels are altered in kidney failure and renal complications of diabetes.
Proper citation: Jason Kim 2019. U Mass - Urea/BUN. protocols.io https://dx.doi.org/10.17504/protocols.io.x5qfq5w Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Hyperinsulinemic-euglycemic clamp is the gold-standard method to assess insulin sensitivity. The hyperinsulinemic-euglycemic clamp is widely used in clinics and laboratories to measure insulin action on glucose utilization in humans and animals for clinical and basic science research. Incorporation of radioactive-labeled glucose during hyperinsulinemic-euglycemic clamps makes it possible to measure glucose metabolism in individual organs in awake mice. Impaired insulin sensitivity (insulin resistance) is a major characteristic of obesity and an early requisite event in the development of type 2 diabetes.
Proper citation: Jason Kim 2019. U Mass - Protein metabolism. protocols.io https://dx.doi.org/10.17504/protocols.io.x4ffqtn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of bilirubin reflect bile acid metabolism.
Proper citation: Jason Kim 2019. U Mass - Bilirubin. protocols.io https://dx.doi.org/10.17504/protocols.io.xscfnaw Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
The EchoMRI 3-in-1 uses ¹H- magnetic resonance spectroscopy to noninvasively measure whole body fat mass, lean mass, and water mass in awake mice. Whole body fat mass is a measure of adiposity that is altered in obesity. Whole body lean mass may be altered by exercise and in mouse models of altered metabolism.
Proper citation: Jason Kim 2019. U Mass - Body composition (whole body). protocols.io https://dx.doi.org/10.17504/protocols.io.xsqfndw Copy
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