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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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U Mass - Cytokines Panel I - multiplex Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xvnfn5e | Mouse Metabolic Phenotyping Centers | Summary: This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity. Cytokines Panel I include IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KC (IL-8 homologue), IL-9, IL-10 (interleukin-10), IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, TNFα (tumor necrosis factor alpha), IFNγ (interferon gamma), IP-10 (interferon gamma-induced protein 10; CXCL-10), Eotaxin (CCL-11), G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte macrophage colony stimulating factor), LIF (leukemia inhibitory factor), LIX (LPS- induced CXC chemokine), MCP-1 (monocyte chemotactic protein-1; CCL-2), M-CSF (macrophage colony stimulating factor), MIG (monokine induced by gamma interferon; CXCL- 9), MIP-1α (macrophage inflammatory protein alpha; CCL-3), MIP-1 (macrophage inflammatory protein beta; CCL-4), and MIP-2/RANTES (macrophage inflammatory protein 2- alpha; CXCL-2). A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=190&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Cytokines Panel I - multiplex. protocols.io https://dx.doi.org/10.17504/protocols.io.xvnfn5e | 2021-04-15 09:14:25 | ||
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U Mass - Albumin Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xprfmm6 | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Albumin is a major circulating protein in body. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=179&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Albumin. protocols.io https://dx.doi.org/10.17504/protocols.io.xprfmm6 | 2021-04-15 09:14:26 | ||
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U Mass - Alkaline Phosphatase Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xptfmnn | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of alkaline phosphatase reflect liver function. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=177&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Alkaline Phosphatase. protocols.io https://dx.doi.org/10.17504/protocols.io.xptfmnn | 2021-04-15 09:14:29 | ||
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U Mass - Body composition (organs) Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xsnfnde | Mouse Metabolic Phenotyping Centers | The EchoMRI 3-in-1 uses ¹H- magnetic resonance spectroscopy to noninvasively measure fat mass, lean mass, and water mass in individual organs. Fat, lean, and water mass composition in specific organs is altered in obesity. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=148&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Body composition (organs). protocols.io https://dx.doi.org/10.17504/protocols.io.xsnfnde | 2021-04-15 09:14:53 | ||
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U Mass - Ammonia Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xq3fmyn | Mouse Metabolic Phenotyping Centers | Summary:This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=172&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Ammonia. protocols.io https://dx.doi.org/10.17504/protocols.io.xq3fmyn | 2021-04-15 09:14:42 | ||
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U Mass - Resistin Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.x4kfquw | Mouse Metabolic Phenotyping Centers | This experiment provides the quantification of multiple hormones using multiplexed-Luminex technology based on beads containing specific antibodies. Sample matrices include plasma, serum, tissue culture supernatants, and tissue or cell lysates. Resistin is an adipocyte-derived hormone (adipokine) that regulates insulin sensitivity and glucose metabolism. Serum resistin levels are altered in obesity, insulin resistance, and type 2 diabetes. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=166&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Resistin. protocols.io https://dx.doi.org/10.17504/protocols.io.x4kfquw | 2021-04-15 09:14:53 | ||
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U Mass - Cholesterol (Total) Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xucfnsw | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of total cholesterol reflect cholesterol metabolism and are associated with cardiovascular disease. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=169&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Cholesterol (Total). protocols.io https://dx.doi.org/10.17504/protocols.io.xucfnsw | 2021-04-15 09:15:34 | ||
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U Mass - Aspartate Transferase Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xr5fm86 | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of aspartate transferase reflect liver function. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=181&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Aspartate Transferase. protocols.io https://dx.doi.org/10.17504/protocols.io.xr5fm86 | 2021-04-15 09:15:13 | ||
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U Mass - Cholesterol (LDL) Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xt9fnr6 | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of LDL-cholesterol reflect cholesterol metabolism and are associated with cardiovascular disease. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=171&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Cholesterol (LDL). protocols.io https://dx.doi.org/10.17504/protocols.io.xt9fnr6 | 2021-04-15 09:15:18 | ||
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U Mass - Hepatic gluconeogenesis Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xy7fpzn | Mouse Metabolic Phenotyping Centers | Summary:Hepatic gluconeogensis is estimated using pyruvate tolerance test that measures systemic elevation of glucose partly derived from pyruvate and hepatic gluconeogensis following an intraperitoneal bolus injection of pyruvate in awake mice. Hepatic gluconeogenesis is affected by obesity and regulates glucose homeostasis. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=144&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Hepatic gluconeogenesis. protocols.io https://dx.doi.org/10.17504/protocols.io.xy7fpzn | 2021-04-15 09:15:29 | ||
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U Mass - Gamma-glutamyl Transferase Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xwzfpf6 | Mouse Metabolic Phenotyping Centers | This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity. Cytokines Panel I include IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KC (IL-8 homologue), IL-9, IL-10 (interleukin-10), IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, TNFα (tumor necrosis factor alpha), IFNγ (interferon gamma), IP-10 (interferon gamma-induced protein 10; CXCL-10), Eotaxin (CCL-11), G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte macrophage colony stimulating factor), LIF (leukemia inhibitory factor), LIX (LPSinduced CXC chemokine), MCP-1 (monocyte chemotactic protein-1; CCL-2), M-CSF (macrophage colony stimulating factor), MIG (monokine induced by gamma interferon; CXCL9), MIP-1α (macrophage inflammatory protein alpha; CCL-3), MIP-1β (macrophage inflammatory protein beta; CCL-4), and MIP-2/RANTES (macrophage inflammatory protein 2alpha; CXCL-2). A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=183&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Gamma-glutamyl Transferase. protocols.io https://dx.doi.org/10.17504/protocols.io.xwzfpf6 | 2021-04-15 09:15:31 | ||
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U Mass - STZ-induced type 1 diabetes model Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xh8fj9w | Mouse Metabolic Phenotyping Centers | Streptozotocin can selectively destroy the pancreatic β-cells with rapid and irreversible necrosis and can be used to generate a chronic model of hyperglycemia and type 1 diabetes. | University of Massachusetts Medical School | https://mmpc.org/shared/document.aspx?id=152&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - STZ-induced type 1 diabetes model. protocols.io https://dx.doi.org/10.17504/protocols.io.xh8fj9w | 2021-04-15 09:15:29 | ||
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U Mass - Hemoglobin A1c Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xy3fpyn | Mouse Metabolic Phenotyping Centers | Summary:This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity. Cytokines Panel I include IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KC (IL-8 homologue), IL-9, IL-10 (interleukin-10), IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, TNFα (tumor necrosis factor alpha), IFNγ (interferon gamma), IP-10 (interferon gamma-induced protein 10; CXCL-10), Eotaxin (CCL-11), G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte macrophage colony stimulating factor), LIF (leukemia inhibitory factor), LIX (LPSinduced CXC chemokine), MCP-1 (monocyte chemotactic protein-1; CCL-2), M-CSF (macrophage colony stimulating factor), MIG (monokine induced by gamma interferon; CXCL9), MIP-1α (macrophage inflammatory protein alpha; CCL-3), MIP-1β (macrophage inflammatory protein beta; CCL-4), and MIP-2/RANTES (macrophage inflammatory protein 2alpha; CXCL-2). A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=160&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Hemoglobin A1c. protocols.io https://dx.doi.org/10.17504/protocols.io.xy3fpyn | 2021-04-15 09:15:45 | ||
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U Mass - Exercise model Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xwyfpfw | Mouse Metabolic Phenotyping Centers | Summary:In-cage free running wheel is used to voluntarily induce exercise in awake mice. Exercise affects insulin sensitivity and glucose metabolism. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=155&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Exercise model. protocols.io https://dx.doi.org/10.17504/protocols.io.xwyfpfw | 2021-04-15 09:16:02 | ||
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U Mass - Adiponectin Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xm9fk96 | Mouse Metabolic Phenotyping Centers | The adiponectin enzyme-linked immunosorbent assay (ELISA) is a standard immunoassay for the detection of total adiponectin levels in serum, adipocyte extracts, and cell culture media samples. This experiment uses a 96-well plate coated with anti-adiponectin antibodies and developed with a secondary anti-adiponectin antibody linked to horseradish peroxidase. The ELISA is developed with 3,3',5,5'-Tetramethylbenzidine (TMB), a chromogenic substrate and read on an automated plate spectrophotometer at 450 nm. Adiponectin is an adipocyte-derived hormone (adipokine) that regulates insulin sensitivity and glucose metabolism. | RRID:AB_2651034 | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=165&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Adiponectin. protocols.io https://dx.doi.org/10.17504/protocols.io.xm9fk96 | 2021-04-15 09:16:07 | |
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U Mass - Creatinine Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xvjfn4n | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of creatinine reflect systemic protein metabolism. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=184&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Creatinine. protocols.io https://dx.doi.org/10.17504/protocols.io.xvjfn4n | 2021-04-15 09:16:14 | ||
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U Mass - Urea/BUN Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.x5qfq5w | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum Urea/BUN levels are affected by alterations in systemic protein and nitrogen metabolism. Serum Urea/BUN levels are altered in kidney failure and renal complications of diabetes. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=187&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Urea/BUN. protocols.io https://dx.doi.org/10.17504/protocols.io.x5qfq5w | 2021-04-15 09:16:25 | ||
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U Mass - Protein metabolism Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.x4ffqtn | Mouse Metabolic Phenotyping Centers | Hyperinsulinemic-euglycemic clamp is the gold-standard method to assess insulin sensitivity. The hyperinsulinemic-euglycemic clamp is widely used in clinics and laboratories to measure insulin action on glucose utilization in humans and animals for clinical and basic science research. Incorporation of radioactive-labeled glucose during hyperinsulinemic-euglycemic clamps makes it possible to measure glucose metabolism in individual organs in awake mice. Impaired insulin sensitivity (insulin resistance) is a major characteristic of obesity and an early requisite event in the development of type 2 diabetes. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=146&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Protein metabolism. protocols.io https://dx.doi.org/10.17504/protocols.io.x4ffqtn | 2021-04-15 09:16:18 | ||
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U Mass - Bilirubin Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xscfnaw | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of bilirubin reflect bile acid metabolism. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=182&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Bilirubin. protocols.io https://dx.doi.org/10.17504/protocols.io.xscfnaw | 2021-04-15 09:16:42 | ||
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U Mass - Body composition (whole body) Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xsqfndw | Mouse Metabolic Phenotyping Centers | The EchoMRI 3-in-1 uses ¹H- magnetic resonance spectroscopy to noninvasively measure whole body fat mass, lean mass, and water mass in awake mice. Whole body fat mass is a measure of adiposity that is altered in obesity. Whole body lean mass may be altered by exercise and in mouse models of altered metabolism. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=147&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Body composition (whole body). protocols.io https://dx.doi.org/10.17504/protocols.io.xsqfndw | 2021-04-15 09:16:43 |
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