Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Authors: G-Biosciences
Group: G-Biosciences
Proper citation: G-Biosciences 2016. CB-X™ Protein Assay for simple aqueous samples. protocols.io https://dx.doi.org/10.17504/protocols.io.e33bgqn Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: This protocol is for the ProteSEEKER™ kit, designed to identify the specific type of proteases present in cell or tissue lysates, and allows determining the minimal number of individual protease inhibitors required for a sample.
Proper citation: G-Biosciences 2017. ProteSEEKER™. protocols.io https://dx.doi.org/10.17504/protocols.io.e5fbg3n Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: MegaLong™ isolates high molecular weight (>100kb) genomic DNA from a variety of samples, including animal tissues, cultured cells, whole blood, bacterial and yeast.The protocol described here is for blood samples. Please refer to the appropriate protocol, depending on your application.
Proper citation: G-Biosciences 2016. MegaLong™ Protocol for Isolation of >100kb Genomic DNA (Blood). protocols.io https://dx.doi.org/10.17504/protocols.io.e8nbhve Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: MegaLong™ isolates high molecular weight (>100kb) genomic DNA from a variety of samples, including animal tissues, cultured cells, whole blood, bacterial and yeast.The protocol described here is for yeast samples. Please refer to the appropriate protocol, depending on your application.
Proper citation: G-Biosciences 2016. MegaLong™ Protocol for Isolation of >100kb Genomic DNA (Yeast DNA). protocols.io https://dx.doi.org/10.17504/protocols.io.e8qbhvw Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.This protocol is for use with nucleated blood cells (bird, fish, frog). Please refer to the appropriate protocol depending on your application.
Proper citation: G-Biosciences 2016. OmniPrep™ For High Quality Genomic DNA Extraction From Nucleated Blood Cells (Bird, Fish, Frog). protocols.io https://dx.doi.org/10.17504/protocols.io.e54bg8w Copy
Authors: G-Biosciences
Group: G-Biosciences
Proper citation: G-Biosciences 2016. CB-X™ Protein Assay for interfering agent removal (sample clean up) and protein estimation. protocols.io https://dx.doi.org/10.17504/protocols.io.e4abgse Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: CasPASE™ Apoptosis Fluorometric Assay provides a simple method for assaying the activities of various caspases (proteases), a key early indicator of apoptosis in mammalian cells.
Proper citation: G-Biosciences 2017. CasPASE™ Apoptosis Fluorometic Assay with Tissue Lysate. protocols.io https://dx.doi.org/10.17504/protocols.io.gyabxse Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: SWIFT™ Western Transfer Pads For Efficient High & Low MW Protein Transfer(Cat. # 786‐370 to 786‐375, 786‐370S, 786‐373S)
Proper citation: G-Biosciences 2016. SWIFT™ Western Transfer Pads. protocols.io https://dx.doi.org/10.17504/protocols.io.e46bgze Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.This protocol is for use with cultured cells. Please refer to the appropriate protocol depending on your application.
Proper citation: G-Biosciences 2016. OmniPrep™ For High Quality Genomic DNA Extraction From Cultured Cells. protocols.io https://dx.doi.org/10.17504/protocols.io.e5xbg7n Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.This protocol is for use with body fluids (including CSF, plasma, saliva, serum, sputum, synovial fluid, urine and whole blood). Please refer to the appropriate protocol depending on your application.
Proper citation: G-Biosciences 2016. OmniPrep™ For High Quality Genomic DNA Extraction From Body Fluids. protocols.io https://dx.doi.org/10.17504/protocols.io.e6jbhcn Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: For routine protein assays, we recommend that researchers generate their own CB-X™ Tables or standard curves. This is a one time commitment to ensure user specific results and allows single tube assays to be performed each time as opposed to generating new calibration plots for each assay. We recommend using a protein standard similar to you protein of interest or a purified source of your protein.The supplied CB-X™ tables may result in some inconsistencies due to the type of cuvettes or microtiter plates used. CB-X™ Table for Spectrophotometer readings were measured with 1cm path length cuvettes against deionized water and CB-X™ Table for Microplate Reader readings were measured using Nunc™ Immuno 96 MicroWell™ Plates (MaxiSorp™ ) [Cat# 442404] against deionized water.
Proper citation: G-Biosciences 2016. Generating CB-X™ Tables and standard curves for CB-X Protein Assay Optimization. protocols.io https://dx.doi.org/10.17504/protocols.io.e4bbgsn Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: This is a collection of protocols for the G-Biosciences CB-X™ Protein Assay.
Proper citation: G-Biosciences 2016. CB-X™ Protein Assays Collection. protocols.io https://dx.doi.org/10.17504/protocols.io.e5mbg46 Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: This is a collection of G-Biosciences MegaLong™ protocols for the isolation of >100kb Genomic DNA .MegaLong™ isolates high molecular weight (>100kb) genomic DNA from a variety of samples, including animal tissues, cultured cells, whole blood, bacterial and yeast. Please refer to the appropriate protocol below, depending on your application.
Proper citation: G-Biosciences 2016. MegaLong™ Protocols Collection for Isolation of >100kb Genomic DNA. protocols.io https://dx.doi.org/10.17504/protocols.io.e7kbhkw Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: This is a collection of protocols for high quality genomic DNA extraction using the OmniPrep™ kit. Please refer to the appropriate protocol below depending on your application.
Proper citation: G-Biosciences 2016. OmniPrep™ Protocol Collection for High Quality Genomic DNA Extraction. protocols.io https://dx.doi.org/10.17504/protocols.io.e7rbhm6 Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: This protocol is part of the FOCUS™ Mitochondria Kit collection. Please refer to the appropriate protocol depending on your application.
Proper citation: G-Biosciences 2016. Isolation of Mitochondria from Soft Tissues (Liver or Brain) using the FOCUS™ Mitochondria Kit. protocols.io https://dx.doi.org/10.17504/protocols.io.e89bhz6 Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: This protocol is part of the FOCUS™ Mitochondria Kit collection. Please refer to the appropriate protocol depending on your application.
Proper citation: G-Biosciences 2016. Fractionation of Light and Heavy Mitochondria by Gradient Cushion using the FOCUS™ Mitochondria Kit. protocols.io https://dx.doi.org/10.17504/protocols.io.e88bhzw Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: This is a protocol for the Isolation of Genomic DNA from Fresh Blood, Bone Marrow & Buffy Coat (0.5ml blood). (Cat. # 786‐294, 786‐295, 786‐296)
Proper citation: G-Biosciences 2016. XIT™ Genomic DNA Blood Kit Protocol for Isolation of Genomic DNA (for 0.5ml blood). protocols.io https://dx.doi.org/10.17504/protocols.io.e5ubg6w Copy
Authors: G-Biosciences
Group: G-Biosciences
Proper citation: G-Biosciences 2016. Collection of FOCUS™ Mitochondria Kit protocols. protocols.io https://dx.doi.org/10.17504/protocols.io.e8tbhwn Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.This protocol is for use with mouse tail tissue. Please refer to the appropriate protocol depending on your application.
Proper citation: G-Biosciences 2016. OmniPrep™ For High Quality Genomic DNA Extraction From Mouse Tail Tissue. protocols.io https://dx.doi.org/10.17504/protocols.io.e6ebhbe Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: This protocol is for processing 20x106 cells (or ~100µl wet cell pellet). It can be scaled up and down accordingly.It is part of the FOCUS™ Mitochondria Kit collection. Please refer to the appropriate protocol depending on your application.
Proper citation: G-Biosciences 2016. Isolation of Mitochondria from Animal Cells using the FOCUS™ Mitochondria Kit. protocols.io https://dx.doi.org/10.17504/protocols.io.e8fbhtn Copy
Can't find your Protocol?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific protocol and you know the DOI of the protocol already, it's easier to enter a DOI to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your protocol in the search results, please help us by adding it into the system — it's easy. Create and publish your protocols at Protocols.io.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
