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ELISA for quantification of granulocyte-macrophage colony-stimulating factor (GM-CSF) in human serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | 10.17504/protocols.io.bj7akrie | University of the West Indies, [email protected] | The granulocyte-macrophage colony-stimulating factor (GM-CSF) was initially classified as a hematopoietic growth factor. Instead, in inflammation, GM-CSF serves as a network of communication between tissue-invading lymphocytes and myeloid cells. Even though lymphocytes are in all likelihood the instigators of chronic inflammatory disease, GM-CSF-activated phagocytes are equipped to cause tissue damage [1].Reference1. Becher B, Tugues S, Greter M. GM-CSF: From Growth Factor to Central Mediator of Tissue Inflammation. Immunity. 2016;45(5):963-973. doi:10.1016/j.immuni.2016.10.026 | University of the West Indies St. Augustine, "Saturnino Lora Torres" Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of granulocyte-macrophage colony-stimulating factor (GM-CSF) in human serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bj7akrie | 2021-03-29 03:15:09 | |||
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Chicken immunization with KLH-gp120 fragment (254-274) conjugate. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjnjkmcn | University of the West Indies, [email protected] | Chicken immunization with peptides is inefective if only just the peptides are being inoculated. However, to make the immune response effective the fragment 254-274 of HIV-1 was conjugated with a carrier protein (KLH) that produced a critical immune response, assessed by ELISA, Immunoblot analysis and dot blot [1-4]. The Polson method (1990) can be used effectively to separate the IgY antibody from the egg yolk of immunized chickens [5].References1. Schade R, Pfister C, Italatsch R, Henklien P (1991) Polyclonal antibodies from chicken egg yolk-an alternative to the production of mammalian IgG type antibodies in rabbits. ATLA 19: 403-419. 2. Justiz Vaillant AA, Akpaka PE, Smikle M, McFarlane-Anderson N (2012) In vitro Inhibition of Staphylococcus aureus Isolates by Anti-Anti-Idiotypic Antibodies to Staphylococcal Protein (SpA). J Vaccines Vaccin 3: 127-131.3. Collins AM, Roberton DM, Hosking CS, Flannery GR (1991) Oral immunization with xenogeneic antibodies stimulates the production of systemic and mucosal anti-idiotypic antibodies. Immunology 73: 388-393. 4. Ho DD, Kaplan JC, Rackauskas IE, Gurney ME (1988) Second conserved domain of gp120 is important for HIV infectivity and antibody neutralization. Science 239: 1021-1023. 5. Polson A (1990) Isolation of IgY from the yolks of eggs by a chloroform polyethylene glycol procedure. Immunol Invest 19: 253-258. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Chicken immunization with KLH-gp120 fragment (254-274) conjugate.. protocols.io dx.doi.org/10.17504/protocols.io.bjnjkmcn | 2021-03-29 03:15:12 | |||
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Preparation of staphylococcal protein-A conjugated to horseradish peroxidase. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj5ykq7w | University of the West Indies, [email protected] | This reagent can be used in ELISA, Western blotting and Dot blot to detect antigens and antibodies. It is important in the immunodiagnosis of infectious diseases. I find this useful in the detection of anti-HIV antibodies by ELISA. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Preparation of staphylococcal protein-A conjugated to horseradish peroxidase.. protocols.io dx.doi.org/10.17504/protocols.io.bj5ykq7w | 2021-03-29 03:15:17 | |||
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ELISA for quantification of human C5 in serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | 10.17504/protocols.io.bj8ckrsw | University of the West Indies, [email protected] | University of the West Indies St. Augustine, "Saturnino Lora Torres' Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human C5 in serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bj8ckrsw | 2021-03-29 03:15:30 | ||||
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ELISA for quantification of tumor necrosis factor alpha (TNF-α) in human serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | 10.17504/protocols.io.bj69krh6 | University of the West Indies, [email protected] | Tumor necrosis factor (TNF) is a cell signaling cytokine involved in systemic inflammation. It is one of the proteins that make up the acute phase reaction [1].Reference1. Pasquereau S, Kumar A, Herbein G. Targeting TNF and TNF Receptor Pathway in HIV-1 Infection: from Immune Activation to Viral Reservoirs.Viruses. 2017;9(4):64. Published 2017 Mar 30. doi:10.3390/v9040064 | University of the West Indies St. Augustine, "Saturnino Lora Torres" Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of tumor necrosis factor alpha (TNF-α) in human serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bj69krh6 | 2021-03-29 03:15:30 | |||
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Determination of IgG concentration by the Mancini test. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj92kr8e | University of the West Indies, [email protected] | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Determination of IgG concentration by the Mancini test.. protocols.io dx.doi.org/10.17504/protocols.io.bj92kr8e | 2021-03-29 03:15:30 | ||||
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Dot Blots Analysis in the Separation of Anti-HIV Antibodies. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjm4kk8w | University of the West Indies, [email protected] | Dot blot frequently is underutilized or is not frequently used because it cannot assess the molecular weight of macromolecules, but it is simple to perform and is useful when a particular biomolecule needs to be identified. In this protocol dot blot analysis is used for detection of anti-HIV antibody [1], and it is also available for determination or separation of any protein. It can be very useful in the immunodiagnosis of infectious diseases caused by viruses [1] and bacteria [2] and in addition for the determination of specific DNA sequences by using probes [3]. References1. Justiz Vaillant AA, McFarlane-Anderson N, Akpaka PE, Smikle MP, Ramirez N, et al. (2013) Use of Dot Blots Analysis in the Separation of Anti-HIV Antibodies in Animals. J Chromat Separation Techniq 4: 181. doi:10.4172/2157- 7064.10001812. Cardona-Castro N, Gotuzzo E, Rodriguez M, Guerra H. Clinical application of a dot blot test for diagnosis of enteric fever due to Salmonella enterica serovar typhi in patients with typhoid fever from Colombia and Peru.Clin Diagn Lab Immunol. 2000;7(2):312-313. doi:10.1128/cdli.7.2.312-313.20003. Clément G, Benhattar J. A methylation sensitive dot blot assay (MS-DBA) for the quantitative analysis of DNA methylation in clinical samples.J Clin Pathol. 2005;58(2):155-158. doi:10.1136/jcp.2004.021147 | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Dot Blots Analysis in the Separation of Anti-HIV Antibodies.. protocols.io dx.doi.org/10.17504/protocols.io.bjm4kk8w | 2021-03-29 03:12:10 | |||
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Isolation of Shigella pathogens from oysters Resource Report Resource Website |
Sade Aisha Folashade John, Patrick E. Akpaka, Chandrashekhar Unakal, Arvind Kurhade, Angel Justiz-Vaillant | 10.17504/protocols.io.bj6pkrdn | University of the West Indies, [email protected] | University of the West Indies St. Augustine, University of the West Indies St. Augustine, University of the West Indies St. Augustine, University of the West Indies St. Augustine, University of the West Indies St. Augustine | 1 | 2020 | Sade Aisha Folashade John, Patrick E. Akpaka, Chandrashekhar Unakal, Arvind Kurhade, Angel Justiz-Vaillant 2020. Isolation of Shigella pathogens from oysters. protocols.io dx.doi.org/10.17504/protocols.io.bj6pkrdn | 2021-03-29 03:12:14 |
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