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Transfection of constructs in diplonemids to enhance the HR pathway, using RS-1 (3-(benzylamino) sulfonyl)-4-bromo-N-(4-bromophenyl) benzamide). Resource Report Resource Website |
Binnypreet Kaur, Drahomíra Faktorová , Julius Lukeš | DOI:10.17504/protocols.io.x45fqy6 | Protist Research to Optimize Tools in Genetics (PROT-G), Julius Lukes | RS-1 (RAD51-stimulatory compound 1) is a stimulator of homologous recombination (HR) protein RAD51 .justify:after { content: ""; display:inline-block; width: 100%; } | [Institute of Parasitology Czech Academy of Sciences Faculty of Sciences University of South Bohemia Czech Republic ], [Institute of Parasitology Czech Academy of Sciences Faculty of Sciences University of South Bohemia Czech Republic], [Institute of Parasitology Czech Academy of Sciences Faculty of Sciences University of South Bohemia Czech Republic ] [Canadian Institute for Advanced Research Toronto Canada] | 3 | 2019 | Binnypreet Kaur, Drahomíra Faktorová , Julius Lukeš 2019. Transfection of constructs in diplonemids to enhance the HR pathway, using RS-1 (3-(benzylamino) sulfonyl)-4-bromo-N-(4-bromophenyl) benzamide).. protocols.io https://dx.doi.org/10.17504/protocols.io.x45fqy6 | 2021-04-15 09:14:30 | |||
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Immunoflourescent assay Resource Report Resource Website |
Binnypreet Kaur1, 2 , Drahomíra Faktorová1, 2, Priscila Peña-Diaz1 and Julius Lukeš1, 2 | DOI:10.17504/protocols.io.hf4b3qw | Protist Research to Optimize Tools in Genetics (PROT-G), Julius Lukes | 1 Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, České Budějovice, Czech Republic 2 Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic, 1 Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, České Budějovice, Czech Republic 2 Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic, 1 Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, České Budějovice, Czech Republic 2 Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic, 1 Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, České Budějovice, Czech Republic 2 Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic, 1 Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, České Budějovice, Czech Republic 2 Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic, 1 Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, České Budějovice, Czech Republic 2 Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic | 1 | 2019 | Binnypreet Kaur1, 2 , Drahomíra Faktorová1, 2, Priscila Peña-Diaz1 and Julius Lukeš1, 2 2019. Immunoflourescent assay. protocols.io https://dx.doi.org/10.17504/protocols.io.hf4b3qw | 2021-04-15 09:14:44 | ||||
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Electroporation of Pseudo-nitzschia multiseries Resource Report Resource Website |
April Woods, G. Jason Smith | DOI:10.17504/protocols.io.hntb5en | Protist Research to Optimize Tools in Genetics (PROT-G), Environmental Biotechnology Lab | Describes novel low conductivity media and multipulse electroporation protocol using a NEPA21 Type II pulse generator with settings optimized to balance transfection efficiency with cell viability for this marine diatom. | Moss Landing Marine Laboratories, Moss Landing Marine Laboratories | 1 | 2017 | April Woods, G. Jason Smith 2017. Electroporation of Pseudo-nitzschia multiseries. protocols.io https://dx.doi.org/10.17504/protocols.io.hntb5en | 2021-04-15 09:14:33 | |||
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Biolistic transformation experiment on Eutreptiella gymnastica, Pyramimonas parkeae and Bigelowiella natans- 1250 psi gene gun Resource Report Resource Website |
Anna Vanclová and Vladimír Hampl | DOI:10.17504/protocols.io.ibvcan6 | Protist Research to Optimize Tools in Genetics (PROT-G) | The protocol describes our attempt to transform three marine protists by biolistic. | Charleu University in Prague | 1 | 2017 | Anna Vanclová and Vladimír Hampl 2017. Biolistic transformation experiment on Eutreptiella gymnastica, Pyramimonas parkeae and Bigelowiella natans- 1250 psi gene gun. protocols.io https://dx.doi.org/10.17504/protocols.io.ibvcan6 | 2021-04-15 09:14:41 | |||
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Euplotes crassus transformation through microinjection into the macronucleus Resource Report Resource Website |
Rachele Cesaroni | DOI:10.17504/protocols.io.g3fbyjn | Protist Research to Optimize Tools in Genetics (PROT-G) | 1 | 2017 | Rachele Cesaroni 2017. Euplotes crassus transformation through microinjection into the macronucleus. protocols.io https://dx.doi.org/10.17504/protocols.io.g3fbyjn | 2021-04-15 09:14:52 | |||||
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Transient transfection of unicellular relative to animals Abeoforma whisleri with electroporation using Neon System Resource Report Resource Website |
Maria Rubio-Brotons | DOI:10.17504/protocols.io.hmvb466 | Protist Research to Optimize Tools in Genetics (PROT-G), Multicellgenomelab | IBE CSIC-UPF | 1 | 2017 | Maria Rubio-Brotons 2017. Transient transfection of unicellular relative to animals Abeoforma whisleri with electroporation using Neon System. protocols.io https://dx.doi.org/10.17504/protocols.io.hmvb466 | 2021-04-15 09:14:46 | ||||
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Transformation of Skeletonema marinoi using Multipulse Electroporation Resource Report Resource Website |
Oskar N. Johansson., Adrian K. Clarke | DOI:10.17504/protocols.io.g6nbzde | Protist Research to Optimize Tools in Genetics (PROT-G) | The following transformation protocol is designed for the insertion of linear DNA constructs into the nuclear genome of Skeletonema marinoi by non-homologous recombination. | BioEnv - University of Gothenburg, BioEnv - University of Gothenburg | 1 | 2017 | Oskar N. Johansson., Adrian K. Clarke 2017. Transformation of Skeletonema marinoi using Multipulse Electroporation. protocols.io https://dx.doi.org/10.17504/protocols.io.g6nbzde | 2021-04-15 09:14:35 | |||
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Culture of unicellular protists in marine broth Resource Report Resource Website |
Maria Rubio-Brotons | DOI:10.17504/protocols.io.gg2btye | Protist Research to Optimize Tools in Genetics (PROT-G), Multicellgenomelab | IBE CSIC-UPF | 3 | 2016 | Maria Rubio-Brotons 2016. Culture of unicellular protists in marine broth. protocols.io https://dx.doi.org/10.17504/protocols.io.gg2btye | 2021-04-15 09:14:35 | ||||
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Electroporation of the protist Cafeteria roenbergensis cells using the Neon Transfection System Resource Report Resource Website |
Sarah Duponchel, Monica Berjon-Otero, Matthias Fischer | DOI:10.17504/protocols.io.qvhdw36 | Protist Research to Optimize Tools in Genetics (PROT-G) | Max-Planck Institute for Medical Research, Max-Planck Institute for Medical Research, Max-Planck Institute for Medical Research | 1 | 2019 | Sarah Duponchel, Monica Berjon-Otero, Matthias Fischer 2019. Electroporation of the protist Cafeteria roenbergensis cells using the Neon Transfection System. protocols.io https://dx.doi.org/10.17504/protocols.io.qvhdw36 | 2021-04-15 09:14:40 | ||||
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Chlorella Virus Plaque Assay Resource Report Resource Website |
Steven Wilhelm | DOI:10.17504/protocols.io.ge2btge | VERVE Net, Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Adapted from: Van Etten, J. (n.d.). Titering of Chlorella Viruses. Retrieved from http://ncv.unl.edu/vanettenlab/ | The University of Tennessee, Knoxville | http://ncv.unl.edu/vanettenlab/Plaque%20assay.pdf | 1 | 2016 | Steven Wilhelm 2016. Chlorella Virus Plaque Assay. protocols.io https://dx.doi.org/10.17504/protocols.io.ge2btge | 2021-04-15 09:14:39 | ||
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RNAqueous with No DNAse, DNA Removal by Acid Phenol:Chloroform Resource Report Resource Website |
Dan Richter | DOI:10.17504/protocols.io.itbcein | Ecology of Marine Plankton (ECOMAP) team - Roscoff, Protist Research to Optimize Tools in Genetics (PROT-G) | March, 2012, based on RNAqueous May 29, 2008 protocol revision C, phenol/chloroform protocol (http://cshprotocols.cshlp.org/content/2010/6/pdb.prot5438.full), ethanol precipitation protocol (http://cshprotocols.cshlp.org/content/2010/6/pdb.prot5440.full) | Richter, Daniel J and Fozouni, Parinaz and Eisen, Michael and King, Nicole. Gene family innovation, conservation and loss on the animal stem lineage. 2018;7:e34226 https://doi.org/10.7554/eLife.34226 | https://elifesciences.org/articles/34226 | 1 | 2017 | Dan Richter 2017. RNAqueous with No DNAse, DNA Removal by Acid Phenol:Chloroform. protocols.io https://dx.doi.org/10.17504/protocols.io.itbcein | 2021-04-15 09:14:53 | ||
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Electroporation of Aurantiochytrium limacinum (ATCC MYA-1381) Resource Report Resource Website |
Mariana Rius | DOI:10.17504/protocols.io.hg6b3ze | Protist Research to Optimize Tools in Genetics (PROT-G), Collier Lab | Stony B | 1 | 2017 | Mariana Rius 2017. Electroporation of Aurantiochytrium limacinum (ATCC MYA-1381). protocols.io https://dx.doi.org/10.17504/protocols.io.hg6b3ze | 2021-04-15 09:15:03 | ||||
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RNA Isolation from Yeast Resource Report Resource Website |
Tara Stuecker | DOI:10.17504/protocols.io.inwcdfe | Protist Research to Optimize Tools in Genetics (PROT-G), Lewis Lab | Protocol for obtaining high quality RNA from yeast using acid-phenol lysis at 65°C. Modifed from DOI: 10.1016/S0076-6879(02)50976-9 to use 10-15 ml of yeast culture. Expected yield of 100-200 μg of RNA. For qPCR or RNA-seq, we recommend DNase-treatment and further purification (e.g. Zymo RNA Purification Kit (R1057)). | Scholes AN, Lewis JA, Comparison of RNA isolation methods on RNA-Seq: implications for differential expression and meta-analyses. BMC Genomics doi: 10.1186/s12864-020-6673-2 | University of Arkansas | https://doi.org/10.1186/s12864-020-6673-2 | 1 | 2017 | Tara Stuecker 2017. RNA Isolation from Yeast. protocols.io https://dx.doi.org/10.17504/protocols.io.inwcdfe | 2021-04-15 09:14:43 | |
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Building up chemostats for experimental eco-evolutionary studies Resource Report Resource Website |
Ana Del Arco, Noemi Woltermann, Lutz Becks | DOI:10.17504/protocols.io.tkxekxn | VERVE Net, Protist Research to Optimize Tools in Genetics (PROT-G) | Chemostats and other flow through culture systems are a powerful tool for the study of microbial and plankton communities in experimental ecology and evolutionary studies. Commercially available chemostat systems allow the control of a large number of parameters (e.g., ph, pressure, CO2 concentration) but are often expensive and offer a high level of control that is often not needed for many experimental studies. Non-commercial chemostats are more cost efficient, easily set up and flexible in volumes used. Different from semi-continuous culture systems, the flow through conditions of chemostats allow a constant inflow of resources from a reservoir (medium bottle) and outflow of unused nutrients, waste products and organisms, which are all collected in a waste bottle. Nutrient levels in the reservoir and the flow rate of the chemostat system (often referred to as dilution rate and presented as the fraction of the volume of the chemostat that is replaced per day) determines population growth rates and dynamics (for the theory behind chemostats see Smith & Waltman, 1995 and Weitz, 2015). Chemostats have been used in a number of studies and with different organisms and combinations of organisms. For example, Boer et al. (2010) studied how growth-limiting intracellular metabolites control yeast growth under diverse nutrient availability (Saccharomyces cerevisiae growing under five different nutrient supply of nitrogen: carbon: phosphorus). Becks et al. (2005) used chemostat systems to show how changes in the flow rate of a chemostat system influences the population dynamics of a three species microbial system. Frickel et al. (2016) used chemostats to investigate eco- evolutionary dynamics in a coevolving host-virus system (algae Chlorella variabilis and Chlorovirus strain PBCV-1) for 90 days.We present here an instruction for a cost efficient and flexible chemostat systems. These chemostats are composed of four main parts: a syringe unit, a glass bottle (i.e. the chemostat), a medium bottle and a waste bottle, all connected by tubing. A peristaltic pump and a low overpressure in the system allow the flow of medium from the reservoir to the chemostat bottle and of unused nutrients, waste products and organisms to the waste bottle. Chemostats are put on stirring plates to create a homogenosus environment within.Becks, L., Hilker, F.M., Malchow, H., Jürgens, K., Arndt, H., 2005. Experimental demonstration of chaos in a microbial food web. Nature 435, 1226–1229. https://doi.org/10.1038/nature03627Boer, V.M., Crutchfield, C.A., Bradley, P.H., Botstein, D., Rabinowitz, J.D., 2010. Growth-limiting Intracellular Metabolites in Yeast Growing under Diverse Nutrient Limitations 21, 198–211. https://doi.org/10.1091/mbc.E09Frickel, J., Sieber, M., Becks, L., 2016. Eco-evolutionary dynamics in a coevolving host – virus system. Ecol. Lett. 19, 450–459. https://doi.org/10.1111/ele.12580Smith, H., Waltman, P., 1995.The Theory of the Chemostat: Dynamics of Microbial Competition (Cambridge Studies in Mathematical Biology). Cambridge: Cambridge University Press. doi:10.1017/CBO9780511530043Weitz, J.S. 2015. Quantitative Viral Ecology. Dynamics of viruses and their microbial host. Princeton University Press. ISBN: 9780691161549 .justify:after { content: ""; display:inline-block; width: 100%; } | Limnological Institute University Konstanz, Aquatic Ecology and Evolution, Konstanz/Egg Germany. , Max Planck for Evolutionary Biology, Plön, Germany , Limnological Institute University Konstanz, Aquatic Ecology and Evolution, Konstanz/Egg Germany. | 2 | 2020 | Ana Del Arco, Noemi Woltermann, Lutz Becks 2020. Building up chemostats for experimental eco-evolutionary studies. protocols.io https://dx.doi.org/10.17504/protocols.io.tkxekxn | 2021-04-15 09:14:41 | |||
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Staining protocol for unicellular protists: Mitotracker and Dapi Resource Report Resource Website |
Maria Rubio-Brotons | DOI:10.17504/protocols.io.gg5bty6 | Protist Research to Optimize Tools in Genetics (PROT-G), Multicellgenomelab | IBE CSIC-UPF | 4 | 2016 | Maria Rubio-Brotons 2016. Staining protocol for unicellular protists: Mitotracker and Dapi. protocols.io https://dx.doi.org/10.17504/protocols.io.gg5bty6 | 2021-04-15 09:14:46 | ||||
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Transformation of Diplonema papillatum using V5+NeoR construct Resource Report Resource Website |
Binnypreet Kaur, Drahomira Faktorova, Julius Lukes Group | DOI:10.17504/protocols.io.4digs4e | Protist Research to Optimize Tools in Genetics (PROT-G), Julius Lukes | p57-V5+NeoR plasmid contains V5-tagged aminoglycoside 3'-phosphotransferase gene (APT; conferring resistance to neomycin) flanked by partial regulatory sequences derived from the hexokinase gene of the kinetoplastid Blastocrithidia (strain p57).Plasmid GenBank accession number = MN047315 | Faktorová D, Nisbet RER, Robledo JAF, Casacuberta E, Sudek L, Allen AE, Ares M, Aresté C, Balestreri C, Barbrook AC, Beardslee P, Bender S, Booth DS, Bouget F, Bowler C, Breglia SA, Brownlee C, Burger G, Cerutti H, Cesaroni R, Chiurillo MA, Clemente T, Coles DB, Collier JL, Cooney EC, Coyne K, Docampo R, Dupont CL, Edgcomb V, Einarsson E, Elustondo PA, Federici F, Freire-Beneitez V, Freyria NJ, Fukuda K, García PA, Girguis PR, Gomaa F, Gornik SG, Guo J, Hampl V, Hanawa Y, Haro-Contreras ER, Hehenberger E, Highfield A, Hirakawa Y, Hopes A, Howe CJ, Hu I, Ibañez J, Irwin NAT, Ishii Y, Janowicz NE, Jones AC, Kachale A, Fujimura-Kamada K, Kaur B, Kaye JZ, Kazana E, Keeling PJ, King N, Klobutcher LA, Lander N, Lassadi I, Li Z, Lin S, Lozano J, Luan F, Maruyama S, Matute T, Miceli C, Minagawa J, Moosburner M, Najle SR, Nanjappa D, Nimmo IC, Noble L, Vanclová AMGN, Nowacki M, Nuñez I, Pain A, Piersanti A, Pucciarelli S, Pyrih J, Rest JS, Rius M, Robertson D, Ruaud A, Ruiz-Trillo I, Sigg MA, Silver PA, Slamovits CH, Smith GJ, Sprecher BN, Stern R, Swart EC, Tsaousis AD, Tsypin L, Turkewitz A, Turnšek J, Valach M, Vergé V, Dassow Pv, Haar Tvd, Waller RF, Wang L, Wen X, Wheeler G, Woods A, Zhang H, Mock T, Worden AZ, Lukeš J, Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nature Methods 17(5). doi: 10.1038/s41592-020-0796-x | 1 Institute of Parasitology, Biology Centre, Czech Academy of Sciences 2 Faculty of Sciences, University of South Bohemia, České Budějovice (Budweis), Czech Republic, Research Scientist, Research Scientist | https://doi.org/10.1038/s41592-020-0796-x | 1 | 2019 | Binnypreet Kaur, Drahomira Faktorova, Julius Lukes Group 2019. Transformation of Diplonema papillatum using V5+NeoR construct. protocols.io https://dx.doi.org/10.17504/protocols.io.4digs4e | 2021-04-15 09:15:04 | |
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Code: Discovery of regulatory motifs in Labyrinthulomycete genomes Resource Report Resource Website |
Joshua Rest, Jackie Collier | DOI:10.17504/protocols.io.hg8b3zw | Protist Research to Optimize Tools in Genetics (PROT-G), Collier Lab | To develop broadly useful methods for the genetic manipulation of Labyrinthulomycetes, it is essential to understand the similarities and differences in regulation of gene expression among them. Toward this end we have used FIMO from the MEME suite (http://meme-suite.org/doc/fimo.html) to identify motifs similar to yeast transcription factor binding sites in each of the three available genome sequences: Aplanochytrium kerguelense PBS07, Schizochytrium aggregatum ATCC 28209, and Aurantiochytrium limacinum ATCC MYA-1381. We then make logograms to illustrate yeast-like GAL4 binding sites in each of the three genomes. | Stony Brook University, Stony Brook University | 1 | 2017 | Joshua Rest, Jackie Collier 2017. Code: Discovery of regulatory motifs in Labyrinthulomycete genomes. protocols.io https://dx.doi.org/10.17504/protocols.io.hg8b3zw | 2021-04-15 09:15:06 | |||
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Co-Incubation protocol for transforming heterotrophic dinoflagellates (e.g. Oxyrrhis marina) Resource Report Resource Website |
Brittany Sprecher, Lu Wang, Huan Zhang, and Senjie Lin | DOI:10.17504/protocols.io.hmzb476 | Protist Research to Optimize Tools in Genetics (PROT-G) | Faktorová D, Nisbet RER, Robledo JAF, Casacuberta E, Sudek L, Allen AE, Ares M, Aresté C, Balestreri C, Barbrook AC, Beardslee P, Bender S, Booth DS, Bouget F, Bowler C, Breglia SA, Brownlee C, Burger G, Cerutti H, Cesaroni R, Chiurillo MA, Clemente T, Coles DB, Collier JL, Cooney EC, Coyne K, Docampo R, Dupont CL, Edgcomb V, Einarsson E, Elustondo PA, Federici F, Freire-Beneitez V, Freyria NJ, Fukuda K, García PA, Girguis PR, Gomaa F, Gornik SG, Guo J, Hampl V, Hanawa Y, Haro-Contreras ER, Hehenberger E, Highfield A, Hirakawa Y, Hopes A, Howe CJ, Hu I, Ibañez J, Irwin NAT, Ishii Y, Janowicz NE, Jones AC, Kachale A, Fujimura-Kamada K, Kaur B, Kaye JZ, Kazana E, Keeling PJ, King N, Klobutcher LA, Lander N, Lassadi I, Li Z, Lin S, Lozano J, Luan F, Maruyama S, Matute T, Miceli C, Minagawa J, Moosburner M, Najle SR, Nanjappa D, Nimmo IC, Noble L, Vanclová AMGN, Nowacki M, Nuñez I, Pain A, Piersanti A, Pucciarelli S, Pyrih J, Rest JS, Rius M, Robertson D, Ruaud A, Ruiz-Trillo I, Sigg MA, Silver PA, Slamovits CH, Smith GJ, Sprecher BN, Stern R, Swart EC, Tsaousis AD, Tsypin L, Turkewitz A, Turnšek J, Valach M, Vergé V, Dassow Pv, Haar Tvd, Waller RF, Wang L, Wen X, Wheeler G, Woods A, Zhang H, Mock T, Worden AZ, Lukeš J, Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nature Methods 17(5). doi: 10.1038/s41592-020-0796-x | University of Connecticut, University of Connecticut, University of Connecticut, University of Connecticut | https://doi.org/10.1038/s41592-020-0796-x | 2 | 2017 | Brittany Sprecher, Lu Wang, Huan Zhang, and Senjie Lin 2017. Co-Incubation protocol for transforming heterotrophic dinoflagellates (e.g. Oxyrrhis marina). protocols.io https://dx.doi.org/10.17504/protocols.io.hmzb476 | 2021-04-15 09:15:10 | ||
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Electroporation of natural communities in sea water Resource Report Resource Website |
Andrés Ramírez, Albane Ruaud, Fernan Federici, Peter von Dassow | DOI:10.17504/protocols.io.h3nb8me | Protist Research to Optimize Tools in Genetics (PROT-G) | This protocol has been optimized for electroporation of natural communities in coastal surface waters. As natural communities may vary in different places, we recommend to use this protocol as a starting point and re-optimize according to the results. Guidelines are provided. | Faktorová D, Nisbet RER, Robledo JAF, Casacuberta E, Sudek L, Allen AE, Ares M, Aresté C, Balestreri C, Barbrook AC, Beardslee P, Bender S, Booth DS, Bouget F, Bowler C, Breglia SA, Brownlee C, Burger G, Cerutti H, Cesaroni R, Chiurillo MA, Clemente T, Coles DB, Collier JL, Cooney EC, Coyne K, Docampo R, Dupont CL, Edgcomb V, Einarsson E, Elustondo PA, Federici F, Freire-Beneitez V, Freyria NJ, Fukuda K, García PA, Girguis PR, Gomaa F, Gornik SG, Guo J, Hampl V, Hanawa Y, Haro-Contreras ER, Hehenberger E, Highfield A, Hirakawa Y, Hopes A, Howe CJ, Hu I, Ibañez J, Irwin NAT, Ishii Y, Janowicz NE, Jones AC, Kachale A, Fujimura-Kamada K, Kaur B, Kaye JZ, Kazana E, Keeling PJ, King N, Klobutcher LA, Lander N, Lassadi I, Li Z, Lin S, Lozano J, Luan F, Maruyama S, Matute T, Miceli C, Minagawa J, Moosburner M, Najle SR, Nanjappa D, Nimmo IC, Noble L, Vanclová AMGN, Nowacki M, Nuñez I, Pain A, Piersanti A, Pucciarelli S, Pyrih J, Rest JS, Rius M, Robertson D, Ruaud A, Ruiz-Trillo I, Sigg MA, Silver PA, Slamovits CH, Smith GJ, Sprecher BN, Stern R, Swart EC, Tsaousis AD, Tsypin L, Turkewitz A, Turnšek J, Valach M, Vergé V, Dassow Pv, Haar Tvd, Waller RF, Wang L, Wen X, Wheeler G, Woods A, Zhang H, Mock T, Worden AZ, Lukeš J, Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nature Methods 17(5). doi: 10.1038/s41592-020-0796-x | Instituto Milenio de Oceanografía, Instituto Milenio de Oceanografía, Instituto Milenio de Oceanografía, Instituto Milenio de Oceanografía | https://doi.org/10.1038/s41592-020-0796-x | 3 | 2018 | Andrés Ramírez, Albane Ruaud, Fernan Federici, Peter von Dassow 2018. Electroporation of natural communities in sea water. protocols.io https://dx.doi.org/10.17504/protocols.io.h3nb8me | 2021-04-15 09:14:50 | |
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Cryopreservation of labyrinthulomycetes in trehalose Resource Report Resource Website |
Jackie Collier | DOI:10.17504/protocols.io.pgtdjwn | Protist Research to Optimize Tools in Genetics (PROT-G), Collier Lab | This method adapted from instructions provided by Daiske Honda, Konan University. | Stony Brook University | 1 | 2018 | Jackie Collier 2018. Cryopreservation of labyrinthulomycetes in trehalose. protocols.io https://dx.doi.org/10.17504/protocols.io.pgtdjwn | 2021-04-15 09:15:16 |
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