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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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Reaction Mixture for M0371 Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cgutwv | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2013/06/10/protocol-for-dephosphorylation-of-5-ends-of-dna-in-restriction-enzyme-reaction-m0371 | 1 | 2015 | New England Biolabs 2015. Reaction Mixture for M0371. protocols.io https://dx.doi.org/10.17504/protocols.io.cgutwv | 2021-04-15 09:15:26 | |||
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Dephosphorylation using rSAP in Restriction Enzyme Reaction(M0371) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cgvtw5 | New England Biolabs (NEB) | This protocol is for dephosphorylation of 5´-ends of DNA using rSAP in restriction enzyme reaction (M0371) | New England Biolabs | https://www.neb.com/protocols/2013/06/10/protocol-for-dephosphorylation-of-5-ends-of-dna-in-restriction-enzyme-reaction-m0371 | 1 | 2015 | New England Biolabs 2015. Dephosphorylation using rSAP in Restriction Enzyme Reaction(M0371). protocols.io https://dx.doi.org/10.17504/protocols.io.cgvtw5 | 2021-04-15 09:15:25 | ||
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NEBNext® Ultra™ DNA Library Prep Protocol for Illumina® With Size Selection (E7370) Resource Report Resource Website |
Isabel Gautreau | DOI:10.17504/protocols.io.gx8bxrw | New England Biolabs (NEB) | This protocol is a group of the following 5 methods from the NEB website:1. NEBNext End Prep 2. Adaptor Ligation3. Size Selection of Adaptor-ligated DNA4. PCR Amplification5. Cleanup of PCR Amplification | New England Biolabs | https://www.neb.com/~/media/Catalog/All-Products/23EF8BC783C749D9B664D90C8C72FA73/Datacards%20or%20Manuals/manualE7370.pdf | 2 | 2017 | Isabel Gautreau 2017. NEBNext® Ultra™ DNA Library Prep Protocol for Illumina® With Size Selection (E7370). protocols.io https://dx.doi.org/10.17504/protocols.io.gx8bxrw | 2021-04-15 09:15:34 | ||
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Immunoprecipitation using Protein A/G Magnetic Beads Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.crav2d | New England Biolabs (NEB) | This is the protocol for immunoprecipitation using Protein A/G Magnetic Beads. | New England Biolabs | https://www.neb.com/protocols/1/01/01/immunoprecipitation-using-protein-ag-magnetic-beads | 1 | 2015 | New England Biolabs 2015. Immunoprecipitation using Protein A/G Magnetic Beads. protocols.io https://dx.doi.org/10.17504/protocols.io.crav2d | 2021-04-15 09:15:32 | ||
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Q5 PCR Mixture for M0302 Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cqqvvv | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2014/08/11/determining-genome-targeting-efficiency-using-t7-endonuclease-i | 1 | 2015 | New England Biolabs 2015. Q5 PCR Mixture for M0302. protocols.io https://dx.doi.org/10.17504/protocols.io.cqqvvv | 2021-04-15 09:15:34 | |||
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PCR with Taq DNA Polymerase (M0273) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.ch7t9m | New England Biolabs (NEB) | This PCR Protocol is for Taq DNA Polymerase with Standard Taq Buffer (M0273) | New England Biolabs | https://www.neb.com/protocols/1/01/01/taq-dna-polymerase-with-standard-taq-buffer-m0273 | 1 | 2015 | New England Biolabs 2015. PCR with Taq DNA Polymerase (M0273). protocols.io https://dx.doi.org/10.17504/protocols.io.ch7t9m | 2021-04-15 09:15:34 | ||
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Ligation Protocol WITH T4 DNA Ligase (M0202) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cdks4v | New England Biolabs (NEB) | Please see the NEB website for more information. | New England Biolabs | https://www.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202 | 1 | 2015 | New England Biolabs 2015. Ligation Protocol WITH T4 DNA Ligase (M0202). protocols.io https://dx.doi.org/10.17504/protocols.io.cdks4v | 2021-04-15 09:15:31 | ||
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Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.p26dqhe | New England Biolabs (NEB) | Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010).Quickly and easily purify up to 100 µg of high-quality total RNA from multiple sample types – all with one kit!For use with blood, cells and tissuesAlso works with tough to lyse samples (bacteria, yeast, plant)Effectively purifies total RNA of all sizes, including small RNAs >20 ntEfficient genomic DNA removal (column and DNase I-based)Contains Proteinase K for processing of tissues and blood samplesIncludes RNA Protection Reagent for sample preservationExcellent valueKit components available separately | New England Biolabs | https://www.neb.com/protocols/2017/11/28/quick-protocol-for-monarch-total-rna-miniprep-kit-neb-t2010 | 2 | 2018 | New England Biolabs 2018. Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010). protocols.io https://dx.doi.org/10.17504/protocols.io.p26dqhe | 2021-04-15 09:15:37 | ||
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High Efficiency Transformation Protocol (C2987H) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.isxcefn | New England Biolabs (NEB) | This is the correct protocol if you are using the C2987H cells. If you are using the C2987I cells, please refer to this protocol. | NEB, Ipswich, MA | https://www.neb.com/protocols/1/01/01/high-efficiency-transformation-protocol-c2987 | 2 | 2018 | New England Biolabs 2018. High Efficiency Transformation Protocol (C2987H). protocols.io https://dx.doi.org/10.17504/protocols.io.isxcefn | 2021-04-15 09:15:51 | ||
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NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB #E7546) Resource Report Resource Website |
Menna Teffera, New England Biolabs | DOI:10.17504/protocols.io.4nngvde | New England Biolabs (NEB) | The NEBNext Ultra II End Repair/dA-Tailing Module is optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5 ́ phosphorylated, 3 ́ dA-tailed ends. This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext™ Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction. This module is also compatible with some Oxford Nanopore MinION™workflows.This module is designed for use with NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600 or #E7535), NEBNext Ultra II Ligation Module (NEB #E7595), and NEBNext Ultra II Q5 Master Mix (NEB #M0544). Kits that include reagents for every step in the Ultra II DNA library construction workflow are also available (NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) and NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB #E7103). | New England Biolabs, New England Biolabs | https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7546.pdf?rev=221a72d9f6624cfda4fc2fe760691f02 | 1 | 2019 | Menna Teffera, New England Biolabs 2019. NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB #E7546). protocols.io https://dx.doi.org/10.17504/protocols.io.4nngvde | 2021-04-15 09:15:53 | ||
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NEBNext End Prep Mixture Resource Report Resource Website |
Isabel Gautreau | DOI:10.17504/protocols.io.cg4tyv | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2012/11/15/nebnext-end-prep-e7370 | 1 | 2015 | Isabel Gautreau 2015. NEBNext End Prep Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.cg4tyv | 2021-04-15 09:15:42 | |||
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NEBNext Ultra II FS DNA Module E7810 Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.n34dgqw | New England Biolabs (NEB) | The NEBNext Ultra II FS DNA Module contains the enzymes and buffers required to convert a broad range of input amounts of intact DNA into fragmented DNA with 5´ phosphorylated 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595) and with the NEBNext Ultra II Q5 Master Mix (NEB #M0544) if amplification is required. The fast, user-friendly workflow has minimal hands on time.Note: The Ultra II FS Module is not compatible with bisulfite conversion workflows | New England Biolabs | 1 | 2018 | New England Biolabs 2018. NEBNext Ultra II FS DNA Module E7810. protocols.io https://dx.doi.org/10.17504/protocols.io.n34dgqw | 2021-04-15 09:15:41 | |||
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In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386) Resource Report Resource Website |
Breton Hornblower | DOI:10.17504/protocols.io.hwkb7cw | New England Biolabs (NEB) | Cas9 Nuclease, S. pyogenes (Cas9), is a double-stranded DNA endonuclease that is guided to its target by sequence complementarity of a small RNA loaded into the protein. This protocol describes how to digest double-stranded DNA in vitro using Cas9 and a single guide RNA (sgRNA). | https://www.neb.com/protocols/2014/05/01/in-vitro-digestion-of-dna-with-cas9-nuclease-s-pyogenes-m0386 | 2 | 2017 | Breton Hornblower 2017. In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386). protocols.io https://dx.doi.org/10.17504/protocols.io.hwkb7cw | 2021-04-15 09:15:54 | |||
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T4 PNK Reaction Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cpevjd | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2012/08/24/radioactive-labeling-with-t4-pnk-or-t4-pnk-3-phosphatase-minus | 1 | 2015 | New England Biolabs 2015. T4 PNK Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.cpevjd | 2021-04-15 09:15:39 | |||
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A-Tailing with Taq Mixture Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.crxv7m | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2013/11/01/a-tailing-with-taq-polymerase | 1 | 2015 | New England Biolabs 2015. A-Tailing with Taq Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.crxv7m | 2021-04-15 09:15:55 | |||
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M0348 Digestion Mixture Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cr5v85 | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/digestion-with-nebnext-dsdna-fragmentase-m0348 | 1 | 2015 | New England Biolabs 2015. M0348 Digestion Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.cr5v85 | 2021-04-15 09:15:56 | |||
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Ligation Protocol with T4 DNA Ligase (M0202) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.irscd6e | New England Biolabs (NEB) | Please see the NEB website for more information. | New England Biolabs | https://www.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202 | 2 | 2017 | New England Biolabs 2017. Ligation Protocol with T4 DNA Ligase (M0202). protocols.io https://dx.doi.org/10.17504/protocols.io.irscd6e | 2021-04-15 09:15:57 | ||
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NEBExpress Ni Resin Gravity Flow Typical Protocol (NEB #S1428) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.bfd7ji9n | New England Biolabs (NEB) | NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.Purification of ≥10 mg His-tagged protein per 1 ml of resinIntended for use in gravity or pressure flow columns, and batch purificationsHigh specific binding of His-tagged proteins yielding purities of >95%Strong nickel ion binding provides excellent resistance to EDTA and reducing agents. Compatible with commercially available detergent-based cell lysis reagentsIsolation and purification of His-tagged fusion proteins under native or denaturing conditions | New England Biolabs | https://www.neb.com/protocols/2019/09/10/nebexpress-ni-resin-gravity-flow-typical-protocol | 1 | 2020 | New England Biolabs 2020. NEBExpress Ni Resin Gravity Flow Typical Protocol (NEB #S1428). protocols.io https://dx.doi.org/10.17504/protocols.io.bfd7ji9n | 2021-04-15 09:16:01 | ||
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Mixture for M0492 Q5 2X Master Mix Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cijucm | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2012/08/29/protocol-for-q5-high-fidelity-2x-master-mix-m0492 | 1 | 2014 | New England Biolabs 2014. Mixture for M0492 Q5 2X Master Mix. protocols.io https://dx.doi.org/10.17504/protocols.io.cijucm | 2021-04-15 09:15:58 | |||
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E7805 NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® Protocol for use with Inputs ≤ 100 ng Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.k8tczwn | New England Biolabs (NEB) | The NEBNext Ultra II FS DNA Module contains the enzymes and buffers required to convert a broad range of input amounts of intact DNA into fragmented DNA with 5´ phosphorylated 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595) and with the NEBNext Ultra II Q5 Master Mix (NEB #M0544) if amplification is required. The fast, user-friendly workflow has minimal hands on time. | New England Biolabs | https://www.neb.com/-/media/nebus/files/manuals/manuale7810.pdf?rev=70d2cafe09b946ceaadff2996ed02c5b&hash=732A8D3E3E8F777DC946F10C3CBC7629 | 1 | 2020 | New England Biolabs 2020. E7805 NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® Protocol for use with Inputs ≤ 100 ng. protocols.io https://dx.doi.org/10.17504/protocols.io.k8tczwn | 2021-04-15 09:16:08 |
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