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Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II DNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of DNA into high quality libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow also has minimal hands-on time.
Proper citation: New England Biolabs 2018. Ultra II DNA Library Prep Kit for Illumina E7645/E7103. protocols.io https://dx.doi.org/10.17504/protocols.io.mc5c2y6 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the Sequential Double Digest Protocol with Standard Restriction Enzymes. If there is no buffer in which the two enzymes exhibit > 50% activity, this sequential digest can be performed.
Proper citation: New England Biolabs 2014. Sequential Double Digest. protocols.io https://dx.doi.org/10.17504/protocols.io.cjmuk5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocol consists of an IgG purification step followed by covalent cross-linking of the IgG to the Protein A/G solid support. For IgG that has been previously purified, proceed directly to the cross-linking protocol.
Proper citation: New England Biolabs 2015. Cross-linking of IgG to Protein A or G Beads (S1425/S1430). protocols.io https://dx.doi.org/10.17504/protocols.io.crdv25 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocols is for PCR using Q5® High-Fidelity DNA Polymerase (M0491)
Proper citation: New England Biolabs 2015. PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (M0493). protocols.io https://dx.doi.org/10.17504/protocols.io.cjtunm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.Purification of ≥10 mg His-tagged protein per 1 ml of resinIntended for use in gravity or pressure flow columns, and batch purificationsHigh specific binding of His-tagged proteins yielding purities of >95%Strong nickel ion binding provides excellent resistance to EDTA and reducing agents. Compatible with commercially available detergent-based cell lysis reagentsIsolation and purification of His-tagged fusion proteins under native or denaturing conditions
Proper citation: New England Biolabs 2020. NEBExpress Ni Resin Batch Binding Typical Protocol (NEB #S1428). protocols.io https://dx.doi.org/10.17504/protocols.io.bfd5ji86 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick Ligation products may be transformed by many different methods. The following protocol is recommended by New England Biolabs.
Proper citation: New England Biolabs 2015. Transformation Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.cpxvpm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the suggested protocol for use with λ DNA-Mono Cut Mix (N3019), фX174 DNA-HaeIII Digest (N3026),pBR322 DNA-BstNI Digest (N3031), pBR322 DNA-MspI Digest (N3032), 2-Log DNA Ladder (0.1-10.0 kb) (N3200), 100 bp DNA Ladder (N3231), 1 kb DNA Ladder (N3232), Low Molecular Weight DNA Ladder (N3233), and 50 bp DNA Ladder (N3236)
Proper citation: New England Biolabs 2015. Suggested protocol for loading a DNA Ladder/marker. protocols.io https://dx.doi.org/10.17504/protocols.io.cq4vyv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocol may be used with electrocompetent cells prepared by you according to this protocol.
Proper citation: New England Biolabs 2015. Electroporation Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.cruv6v Copy
Authors: New England Biolabs
Summary: This is a protocol for a typical DNase I Reaction, using the M0303 RNase-free DNase I.
Proper citation: New England Biolabs 2021. A Typical DNase I Reaction (M0303). protocols.io https://dx.doi.org/10.17504/protocols.io.bby9ipz6 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) depletes both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications.
Proper citation: New England Biolabs 2018. Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765). protocols.io https://dx.doi.org/10.17504/protocols.io.wkdfcs6 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocol can be used to add As to the blunt-ends of DNA fragments that have been amplified using a high-fidelity polymerase (such as Q5® High Fidelity DNA Polymerase).
Proper citation: New England Biolabs 2015. A-Tailing with Taq Polymerase. protocols.io https://dx.doi.org/10.17504/protocols.io.crvv65 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Mixture for M0267 Taq with ThermoPol. protocols.io https://dx.doi.org/10.17504/protocols.io.cigubv Copy
Authors: New England Biolabs
Proper citation: New England Biolabs 2020. NEBExpress Ni-NTA Magnetic Beads (NEB #S1423). protocols.io https:// Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2014. M0368 RNA and Primer mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.chut6v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Multiplex Small RNA Library Prep Set for Illumina contains the adaptors, primers, enzymes and buffers required to convert small RNAs into indexed libraries for next generation sequencing on the Illumina platform. The novel workflow has been optimized to minimized adaptor dimers, while producing high-yield, high-diversity libraries. The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol. Minimized adaptor-dimer contaminationHigh yieldsInput RNA can be Total RNASuitable for methylated small RNAs (e.g., piRNAs) as well as unmethylated small RNAsMultiplex Small RNA Library Prep Workflow This kit includes a novel protocol that results in higher yields and lower adaptor-dimer contamination.
Proper citation: New England Biolabs 2018. Protocol for use with NEBNext® Small RNA Library Prep Set for Illumina® (E7300, E7580, E7560, E7330). protocols.io https://dx.doi.org/10.17504/protocols.io.t7qermw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
Proper citation: New England Biolabs 2018. Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030). protocols.io https://dx.doi.org/10.17504/protocols.io.nyfdftn Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB), Coronavirus Method Development Community
Summary: This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®), NEB #E7658S/L 24/96 reactions.
Proper citation: New England Biolabs 2021. NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®) E7658. protocols.io https://dx.doi.org/10.17504/protocols.io.bsy5nfy6 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is a generic PNGase F protocol with denaturing reaction conditions. It is appropriate for both P0704 and P0708.
Proper citation: New England Biolabs 2015. PNGase F Protocol, Denaturing Conditions. protocols.io https://dx.doi.org/10.17504/protocols.io.cqfvtm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the correct protocol if you are using the C3019I cells. If you are using the C3019H cells, please refer to this protocol.
Proper citation: New England Biolabs 2015. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019I). protocols.io https://dx.doi.org/10.17504/protocols.io.cf5tq5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick CIP is a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.Rapid and irreversible heat inactivation eliminates unwanted activityImproved storage stability versus native enzymeFaster reaction setup (no supplemental additives like zinc required) and shorter incubation timeFlexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)Less enzyme required (high specific activity), resulting in a lower cost per reactionNo need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysisRecombinant for purity, consistency and value
Proper citation: New England Biolabs 2020. Protocol for Dephosphorylation of 5′ ends of DNA using Quick CIP (NEB #M0525). protocols.io https://dx.doi.org/10.17504/protocols.io.7r2hm8e Copy
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