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Universal sandwich enzyme linked immunosorbent assay for investigating protein-LA (SpLA) interactions with immunoglobulins using a SpG-HRP conjugate. Resource Report Resource Website |
Angel Justiz-Vaillant, Norma McFarlane-Anderson | DOI:10.17504/protocols.io.bjpzkmp6 | University of the West Indies, [email protected] | University of the West Indies St. Augustine, University of West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Universal sandwich enzyme linked immunosorbent assay for investigating protein-LA (SpLA) interactions with immunoglobulins using a SpG-HRP conjugate.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjpzkmp6 | 2021-04-15 09:15:34 | ||||
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Preparation of horseradish peroxidase (HRP) conjugated Streptococcal protein-G by the periodate method. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bjkukkww | University of the West Indies, [email protected] | Streptococcal protein G, type III bacterial Fc receptor, is a globular protein produced by Streptococcal species and is composed of three nearly identical domains, each of 55 amino acids. SpG binds the Fc regions of antibodies IgG from many mammalian species [1-4]. The amino acid sequence of the IgG-binding regions (C1, C2 and C3) of SpG are not homologous to those of the regions (E, D, A, B and C) of SpA. A prerequisite of SpG for successful binding affinity to many IgG is the possession of at least two domains [3].Streptococcal protein G has been shown to have high binding affinity to sera from various mammalian species including human, , cow, pig, rabbit, goat and sheep by using competitive RIA [5] and to cervids, giraffes and peccaries by direct ELISA [2]. More recently using direct ELISA, some new interactions of SpG with free-ranging nondomestic hoofstock (order Artiodactyla) such as addax, bontebok, antelope, bison, elk, impala, kudu/nyala, oryx, muntjac, sheep, and white-tailed deer have been reported [1,2]. SpG does not bind to the Fc region of the immunoglobulin Y (IgY) of avian species [6]. This reagent can be used in ELISA, Western blotting oand Dot blot to detect antigens and antibodies [1]. It is important in the immunodiagnosis of infectious diseases and other problems. Protein L binds to kappa light chains of immunoglobulins from many animal species including human, mouse, rat, chicken, hamster and pig [1].1. Justiz-Vaillant AA, Akpaka PE, McFarlane-Anderson N, Smikle MF. Comparison of techniques of detecting immunoglobulin-binding protein reactivity to immunoglobulin produced by different avian and mammalian species.West Indian Med J. 2013;62(1):12-20.2. Stöbel K, Schönberg A, Staak C. A new non-species dependent ELISA for detection of antibodies to Borrelia burgdorferi s. l. in zoo animals.Int J Med Microbiol. 2002;291 Suppl 33:88-99. doi:10.1016/s1438-4221(02)80018-2.3. Kronvall G. A surface component in group A, C, and G streptococci with non-immune reactivity for immunoglobulin G. J Immunol 1973, 111(5): 1401-6.4. Justiz-Vaillant AA, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 2013, 4: 175.5. Reis KJ, Ayoub EM, Boyle MD. Streptococcal Fc receptors. II. Comparison of the reactivity of a receptor from a group C streptococcus with staphylococcal protein A. J Immunol 1984, 132(6): 3098-102.6. Serhir B, Dubreuil D, Higgins R, Jacques M. Purification and characterization of a 52-kilodalton immunoglobulin G-binding protein from Streptococcus suis capsular type 2. J Bacteriol 1995, 177(13): 3830-6. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Preparation of horseradish peroxidase (HRP) conjugated Streptococcal protein-G by the periodate method.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjkukkww | 2021-04-15 09:15:36 | |||
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Purification of avian egg yolk immunoglobulins using the water dilution method, precipitation with isopropanol and using HiTrap™ Columns. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bkbnksme | University of the West Indies, [email protected] | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Purification of avian egg yolk immunoglobulins using the water dilution method, precipitation with isopropanol and using HiTrap™ Columns.. protocols.io https://dx.doi.org/10.17504/protocols.io.bkbnksme | 2021-04-15 09:15:37 | ||||
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ELISA for quantification of granulocyte-macrophage colony-stimulating factor (GM-CSF) in human serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | DOI:10.17504/protocols.io.bj7akrie | University of the West Indies, [email protected] | The granulocyte-macrophage colony-stimulating factor (GM-CSF) was initially classified as a hematopoietic growth factor. Instead, in inflammation, GM-CSF serves as a network of communication between tissue-invading lymphocytes and myeloid cells. Even though lymphocytes are in all likelihood the instigators of chronic inflammatory disease, GM-CSF-activated phagocytes are equipped to cause tissue damage [1].Reference1. Becher B, Tugues S, Greter M. GM-CSF: From Growth Factor to Central Mediator of Tissue Inflammation. Immunity. 2016;45(5):963-973. doi:10.1016/j.immuni.2016.10.026 | University of the West Indies St. Augustine, "Saturnino Lora Torres" Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of granulocyte-macrophage colony-stimulating factor (GM-CSF) in human serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj7akrie | 2021-04-15 09:15:14 | |||
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Chicken immunization with KLH-gp120 fragment (254-274) conjugate. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bjnjkmcn | University of the West Indies, [email protected] | Chicken immunization with peptides is inefective if only just the peptides are being inoculated. However, to make the immune response effective the fragment 254-274 of HIV-1 was conjugated with a carrier protein (KLH) that produced a critical immune response, assessed by ELISA, Immunoblot analysis and dot blot [1-4]. The Polson method (1990) can be used effectively to separate the IgY antibody from the egg yolk of immunized chickens [5].References1. Schade R, Pfister C, Italatsch R, Henklien P (1991) Polyclonal antibodies from chicken egg yolk-an alternative to the production of mammalian IgG type antibodies in rabbits. ATLA 19: 403-419. 2. Justiz Vaillant AA, Akpaka PE, Smikle M, McFarlane-Anderson N (2012) In vitro Inhibition of Staphylococcus aureus Isolates by Anti-Anti-Idiotypic Antibodies to Staphylococcal Protein (SpA). J Vaccines Vaccin 3: 127-131.3. Collins AM, Roberton DM, Hosking CS, Flannery GR (1991) Oral immunization with xenogeneic antibodies stimulates the production of systemic and mucosal anti-idiotypic antibodies. Immunology 73: 388-393. 4. Ho DD, Kaplan JC, Rackauskas IE, Gurney ME (1988) Second conserved domain of gp120 is important for HIV infectivity and antibody neutralization. Science 239: 1021-1023. 5. Polson A (1990) Isolation of IgY from the yolks of eggs by a chloroform polyethylene glycol procedure. Immunol Invest 19: 253-258. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Chicken immunization with KLH-gp120 fragment (254-274) conjugate.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjnjkmcn | 2021-04-15 09:15:41 | |||
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Enzyme linked immunosorbent assay for investigating the binding of Protein-L to diverse immunoglobulins Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bjpfkmjn | University of the West Indies, [email protected] | This SpL ELISA can be used to detect specific antibodies in various animal species including human, mouse, rat, dog, rabbit, chicken, monkey, pig and hamster [1].1. De Chateau M, Nilson BH, Erntell M, Myhre E, Magnusson CG, Akerstrom B et al. On the interaction between protein L and immunoglobulins of various mammalian species. Scand J Immunol 1993; 37: 399−405 | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Enzyme linked immunosorbent assay for investigating the binding of Protein-L to diverse immunoglobulins . protocols.io https://dx.doi.org/10.17504/protocols.io.bjpfkmjn | 2021-04-15 09:15:19 | |||
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ELISA for quantification of human C5 in serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | DOI:10.17504/protocols.io.bj8ckrsw | University of the West Indies, [email protected] | University of the West Indies St. Augustine, "Saturnino Lora Torres' Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human C5 in serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj8ckrsw | 2021-04-15 09:15:17 | ||||
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ELISA for quantification of tumor necrosis factor alpha (TNF-α) in human serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | DOI:10.17504/protocols.io.bj69krh6 | University of the West Indies, [email protected] | Tumor necrosis factor (TNF) is a cell signaling cytokine involved in systemic inflammation. It is one of the proteins that make up the acute phase reaction [1].Reference1. Pasquereau S, Kumar A, Herbein G. Targeting TNF and TNF Receptor Pathway in HIV-1 Infection: from Immune Activation to Viral Reservoirs.Viruses. 2017;9(4):64. Published 2017 Mar 30. doi:10.3390/v9040064 | University of the West Indies St. Augustine, "Saturnino Lora Torres" Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of tumor necrosis factor alpha (TNF-α) in human serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj69krh6 | 2021-04-15 09:15:45 | |||
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ELISA for anti-HIV peptide antibodies in the egg yolks of brown Leghorn layer hens. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bjiqkkdw | University of the West Indies, [email protected] | These are three ELISAs for detection of anti-HIV antibodies. These test were highly specific, sensitive and reproducible. They detect specific immunoglobulin Y against HIV peptides in the egg yolk of brown Leghorn layer hens. A modification of these methods were used to detect anti-HIV antibodies in Humans. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for anti-HIV peptide antibodies in the egg yolks of brown Leghorn layer hens.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjiqkkdw | 2021-04-15 09:15:45 | |||
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Direct ELISA for investigating the binding of recombinant or chemically-made Protein-AG to immunoglobulins. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bjxwkppe | University of the West Indies, [email protected] | Protein AG (SpAG) is an immunoglobulin-binding protein that interacts with the Fc regions of many mammalian immunoglobulins [1].References1.Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175 | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Direct ELISA for investigating the binding of recombinant or chemically-made Protein-AG to immunoglobulins.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjxwkppe | 2021-04-15 09:15:17 | |||
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Binding of avian immunoglobulins to peroxidase-labeled anti-chicken IgY conjugate by double immunodiffusion (Ouchterlony) technique. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bjsiknce | University of the West Indies, [email protected] | In this experiment anti-chicken IgY developed in rabbit cross-reacts with a large panel of avian species including ostrich, duck, pigeon, and quail. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Binding of avian immunoglobulins to peroxidase-labeled anti-chicken IgY conjugate by double immunodiffusion (Ouchterlony) technique.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjsiknce | 2021-04-15 09:15:46 | |||
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ELISA for quantification of IL-7 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bj26kqhe | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Bone marrow stromal cells produce IL-7 that acts on pre-B cells and T cells. It causes B-cell and T-cell proliferation.[1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-7 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj26kqhe | 2021-04-15 09:15:47 | |||
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ELISA for quantification of human immunoglobulin E (IgE) in serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | DOI:10.17504/protocols.io.bj7xkrpn | University of the West Indies, [email protected] | IgE is a monomer. It has a molecular weight of 188 Kd and a serum concentration of 0.00005 mg/mL. It protects against parasites and also binds to high-affinity receptors on mast cells and basophils causing allergic reactions. [1] | University of the West Indies St. Augustine, "Saturnino Lora Torres' Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human immunoglobulin E (IgE) in serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj7xkrpn | 2021-04-15 09:15:47 | |||
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Preparation of a protein-AG conjugated to horseradish peroxidase by the periodate method. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bjk6kkze | University of the West Indies, [email protected] | A recombinant protein that combines the IgG-binding domains of SpA and SpG was developed, and labelled to horseradish peroxidase. It was used as universal conjugate in ELISA for the assessment of antibodies against Brucella spp in cattle, sheep, dogs, goats and pigs. It was reported that similar results as the one shown using the chimeric protein AG were obtained when murine monoclonal antibody-enzyme conjugates were used [1,2]. References1. Justiz-Vaillant AA, Akpaka PE, McFarlane-Anderson N, Smikle MF. Comparison of techniques of detecting immunoglobulin-binding protein reactivity to immunoglobulin produced by different avian and mammalian species.West Indian Med J. 2013;62(1):12-20.2. Justiz-Vaillant AA, McFarlane-Anderson N, and Smikle M. “Bacterial Immunoglobulin (Ig)-Receptors: Past and Present Perspectives.” American Journal of Microbiological Research, vol. 5, no. 2 (2017): 44-50. doi: 10.12691/ajmr-5-2-4. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Preparation of a protein-AG conjugated to horseradish peroxidase by the periodate method.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjk6kkze | 2021-04-15 09:15:23 | |||
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ELISA for quantification of IL-15 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bkepktdn | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Monocytes, epithelium, and muscles make IL-15. It acts on T cells and activated B cells. It causes the proliferation of both B and T cells. It causes NK cell memory and CD8+ T cell proliferation. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-15 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bkepktdn | 2021-04-15 09:15:28 | |||
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ELISA for quantification of human C6 in serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | DOI:10.17504/protocols.io.bj8bkrsn | University of the West Indies, [email protected] | University of the West Indies St. Augustine, "Saturnino Lora Torres' Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human C6 in serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj8bkrsn | 2021-04-15 09:15:26 | ||||
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ELISA for quantification of human C9 in serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | DOI:10.17504/protocols.io.bj79krr6 | University of the West Indies, [email protected] | University of the West Indies St. Augustine, "Saturnino Lora Torres' Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human C9 in serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj79krr6 | 2021-04-15 09:15:33 | ||||
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ELISA for quantification of IL-37 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bj4bkqsn | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. IL-37 plays an essential role in the regulation of the innate immunity causing immunosuppression. Phagocytes and organs including the uterus, testis, and thymus express it. IL-37 upregulates immune responses and inflammation in autoimmune disorders. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-37 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj4bkqsn | 2021-04-15 09:15:32 | |||
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Determination of IgM concentration by the Mancini test. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bj9zkr76 | University of the West Indies, [email protected] | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Determination of IgM concentration by the Mancini test.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj9zkr76 | 2021-04-15 09:15:52 | ||||
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Copy of Copy of ELISA for quantification of human C6 in serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | DOI:10.17504/protocols.io.bj77krrn | University of the West Indies St. Augustine, "Saturnino Lora Torres' Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. Copy of Copy of ELISA for quantification of human C6 in serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj77krrn | 2021-04-15 09:15:39 |
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