Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Dephosphorylation of 5´-ends of DNA using rSAP (M0371) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.crkv4v | New England Biolabs (NEB) | Protocol for Dephosphorylation of 5´-ends of DNA using rSAP (M0371) | New England Biolabs | https://www.neb.com/protocols/2013/06/10/protocol-for-dephosphorylation-of-5-ends-of-dna-m0371 | 1 | 2015 | New England Biolabs 2015. Dephosphorylation of 5´-ends of DNA using rSAP (M0371). protocols.io dx.doi.org/10.17504/protocols.io.crkv4v | 2021-03-29 03:09:31 | ||
|
ZWEK alloy fabrication procedure Resource Report Resource Website |
Lumei Liu, Youngmi Koo, Boyce Collins, Zhigang Xu, Jagannathan Sankar, Yeoheung Yun | 10.17504/protocols.io.iepcbdn | Melting and alloying operations were performed in a low carbon steel crucible in an inert ultra-high purity argon environment, preventing the oxidation reactions of molten Mg. High purity Mg (99.97%) was heated to 710°C for 10 minutes until the slug was completely melted. Each alloying element was added separately and kept for 10 minutes before additional alloy was added to ensure complete dissolution of all alloying elements. Automated stirring was applied for 15 minutes to ensure a homogeneous distribution of elements throughout the melt. The melt was then raised to 730 °C, held for 15 minutes and then poured into a steel tube mold. A solution heat treat was carried out on the as-cast alloy at 510 °C for 10 h before it was extruded. The extrusion was conducted with an extrusion ratio 10, extrusion temperature 400 ºC and ramp speed 1 mm/s. | Liu L, Koo Y, Collins B, Xu Z, Sankar J, Yun Y (2017) Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic system. PLoS ONE 12(8): e0182914. doi: 10.1371/journal.pone.0182914 | National Science Foundation-Engineering Research Center for Revolutionizing Metallic Biomaterials, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA FIT BEST Laboratory, Department of Chemical, Biological, and Bioengineering, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA, National Science Foundation-Engineering Research Center for Revolutionizing Metallic Biomaterials, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA FIT BEST Laboratory, Department of Chemical, Biological, and Bioengineering, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA, National Science Foundation-Engineering Research Center for Revolutionizing Metallic Biomaterials, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA FIT BEST Laboratory, Department of Chemical, Biological, and Bioengineering, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA, National Science Foundation-Engineering Research Center for Revolutionizing Metallic Biomaterials, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA FIT BEST Laboratory, Department of Chemical, Biological, and Bioengineering, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA, National Science Foundation-Engineering Research Center for Revolutionizing Metallic Biomaterials, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA FIT BEST Laboratory, Department of Chemical, Biological, and Bioengineering, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA, National Science Foundation-Engineering Research Center for Revolutionizing Metallic Biomaterials, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA FIT BEST Laboratory, Department of Chemical, Biological, and Bioengineering, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA | https://doi.org/10.1371/journal.pone.0182914 | 1 | 2017 | Lumei Liu, Youngmi Koo, Boyce Collins, Zhigang Xu, Jagannathan Sankar, Yeoheung Yun 2017. ZWEK alloy fabrication procedure. protocols.io dx.doi.org/10.17504/protocols.io.iepcbdn | 2021-03-29 03:11:54 | ||
|
Blasticidin S HCl in Mammalian Culture Determine Working Concentration Resource Report Resource Website |
Sean Seaver | 10.17504/protocols.io.cikucv | The working concentration of blasticidin S HCl for use in mammalian cell gene selection can range anywhere from 2-100μg/mL depending on the cell lines used.Optimal doses typically range from 2-20μg/mLHowever, we recommend performing a kill curve to determine the optimal dose for your cell lines. | P212121 | http://store.p212121.com/blasticidin-s-hcl/ | 1 | 2014 | Sean Seaver 2014. Blasticidin S HCl in Mammalian Culture Determine Working Concentration. protocols.io dx.doi.org/10.17504/protocols.io.cikucv | 2021-03-29 03:11:54 | |||
|
Preparation of electrocompetent cells Resource Report Resource Website |
Josef Hoff | 10.17504/protocols.io.u3heyj6 | Preparation of electrocompetent cells Weinstock paper:Matthew T Weinstock, Eric D Hesek,Christopher M Wilson, Daniel G GibsonVibrio natriegens as a fast-growing host for molecular biology Nature Methods volume 13, pages 849–851 (2016)To prepare the day before: -LB I media +V2 salts (204 mM NaCl, 4.2 mM KCl, 23.14mM MgCl2 )-electroporation buffer (680 mM sucrose, 7 mM K2HPO4, pH 7) ( sterile filtrated)-over night culture of V.n from the cryo-stock in LBI + v2 salts (37 °C ; at 200 r.p.m)Preparing culture :Depending on how many aliquds you want to have, incubate media with your overnight culture for a starting OD of 0.05.The culture is grown at 37 °C in a baffled flask, shaking at 200 r.p.m. until an OD600 between 0.5 to 0.8 is reached. *be careful when they reach an OD near 0.1, V. natriegensis very fast growing, so start measuring in shorter time periods.Prechill the electroporation bufferWashing:From here on try always to keep your culture on ice.-The culture is then put on ice for 15 min. ( the original Protocol sais to directly fill them into your prechilled centrifugatin containments )-The cells are pelleted at 3000x g. for 20 min at 4 °C.-The supernatant is carefully decanted and the cell pellets are gently suspended in 10mL of chilled electroporation buffer.-The suspensions are transferred to a chilled50mL falcon tube and the tube is filled top with additional chilled electroporation buffer (50mL) and inverted several times. -The cells are centrifuged down at 3000x g for 15 min at 4 °C. -The wash is repeated two times for a total of three washes.Aliquotation:After the final wash, the supernant is carefully decanted the cells are gently resuspended in residual electroporation buffer. Measure the OD in a 1/20 dilution against electroporation buffer.The volume is adjusted with additional electroporation buffer to bring the final OD600 to 16.The Cells are aliquoted ( 80µL ) into chilled 1.5µL centrifugation tubes, directly frozen in liquid nitrogen and stored at −80 °C until use. | Phillips University | 1 | 2018 | Josef Hoff 2018. Preparation of electrocompetent cells. protocols.io dx.doi.org/10.17504/protocols.io.u3heyj6 | 2021-03-29 03:11:54 | ||||
|
Leaf Protein Extraction for Immunoblot (Soybean, Cowpea, Tobacco) Resource Report Resource Website |
Steven Burgess | 10.17504/protocols.io.bqnmmvc6 | Ag SynBio Lab UIUC | This protein extraction protocol was developed for analysis of protein abundance in leaf tissue by immunoblot. It was optimized for dicot species including Glycine max.Quantities - 450 µL protein extraction buffer (PEB) per three 13.4 mm diameter leaf disks (size #7 Humboldt Cork Borer)Note from Agrisera: Keeping sample volumes in a range of 0.2-0.5 mL has been found to contribute to better extraction results, an upscale in volume is not recommended, if no cork borer available the suggestion is 500 µL PEB per 100 mg of plant tissue. - Final volume ~400 µL - Expected yield: 1.5 - 6 µg / µL - Total yield: 6 - 24 mg protein - Amount of total soluble protein to load per lane 30 µg Note: when using the TissueLyser II it is recommended to use 2 mL centrifuge tubes in conjunction with 4mm beads. 1.5mL tubes are narrow at the end and samples will not grind properly if you use the 4mm beads. | University of Illinois at Urbana-Champaign | 2 | 2020 | Steven Burgess 2020. Leaf Protein Extraction for Immunoblot (Soybean, Cowpea, Tobacco). protocols.io dx.doi.org/10.17504/protocols.io.bqnmmvc6 | 2021-03-29 03:11:54 | |||
|
Protocol for bacterial depletion of Aiptasia anemones - Towards the generation of gnotobiotic/germ-free cnidarian host animals Resource Report Resource Website |
Ruben M Costa, Anny Cárdenas, Christian Voolstra | 10.17504/protocols.io.7fthjnn | reefgenomics, Aiptasia-Symbiodiniaceae Model System | This protocol aims to obtain bacteria-depleted Aiptasia polyps. It is divided into 2 sections: (1) a rearing protocol composed of sterile food preparation and anemone priming and (2) a bacteria-depletion protocol for Aiptasia. | Red Sea Research Center, Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia, Department of Biology, University of Konstanz, 78457 Konstanz, Germany, Department of Biology, University of Konstanz, 78457 Konstanz, Germany | 1 | 2019 | Ruben M Costa, Anny Cárdenas, Christian Voolstra 2019. Protocol for bacterial depletion of Aiptasia anemones - Towards the generation of gnotobiotic/germ-free cnidarian host animals. protocols.io dx.doi.org/10.17504/protocols.io.7fthjnn | 2021-03-29 03:11:55 | |||
|
Digestion with NEBNext dsDNA Fragmentase (M0348) Resource Report Resource Website |
Isabel Gautreau | 10.17504/protocols.io.cr4v8v | New England Biolabs (NEB) | Protocol for digestion with NEBNext dsDNA Fragmentase (M0348) | New England Biolabs | https://www.neb.com/protocols/1/01/01/digestion-with-nebnext-dsdna-fragmentase-m0348 | 1 | 2015 | Isabel Gautreau 2015. Digestion with NEBNext dsDNA Fragmentase (M0348). protocols.io dx.doi.org/10.17504/protocols.io.cr4v8v | 2021-03-29 03:11:55 | ||
|
Sample Informed Consent Form Template (Appendix C of Phase 3 study of Vaccine Candidate for COVID-19) Resource Report Resource Website |
Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores | 10.17504/protocols.io.bj6dkra6 | Coronavirus Method Development Community, PATH | This is Appendix C of "Phase 3 randomized, double-blinded, placebo-controlled trial to evaluate the safety, immunogenicity, and efficacy of Vaccine Candidate against COVID-19 in adults > 18 years of age"This generic Phase 3 protocol was developed by the PATH team with support of the Bill and Melinda Gates Foundation. The aim of the collection is to share recommended best practices in designing and implementing a Phase 3 study of a COVID-19 vaccine candidate. As Phase 3 trials of different Vaccine Candidates proceed around the world, following the same protocols will ensure consistency and comparability of the Phase 3 trial results.Please note that this is an evolving document, to be versioned and updated, based on community feedback and new data. | Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington) | 1 | 2020 | Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores 2020. Sample Informed Consent Form Template (Appendix C of Phase 3 study of Vaccine Candidate for COVID-19). protocols.io dx.doi.org/10.17504/protocols.io.bj6dkra6 | 2021-03-29 03:11:56 | |||
|
Zika Virus propagation Resource Report Resource Website |
Elen Mello de Souza, Audrien Alves Andrade, Fernanda Cunha Jácome, Marcos Alexandre Nunes da Silva, Gabriela Cardoso Caldas, Ortrud Monika Barth, Debora Ferreira Barreto-Vieira | 10.17504/protocols.io.jkackse | Zika Virus propagation | Morphology and Morphogenesis Laboratory, Instituto Oswaldo Cruz, Fiocruz, Morphology and Morphogenesis Laboratory, Instituto Oswaldo Cruz, Fiocruz, Morphology and Morphogenesis Laboratory, Instituto Oswaldo Cruz, Fiocruz, Morphology and Morphogenesis Laboratory, Instituto Oswaldo Cruz, Fiocruz, Morphology and Morphogenesis Laboratory, Instituto Oswaldo Cruz, Fiocruz, Morphology and Morphogenesis Laboratory, Instituto Oswaldo Cruz, Fiocruz, Morphology and Morphogenesis Laboratory, Instituto Oswaldo Cruz, Fiocruz | 1 | 2017 | Elen Mello de Souza, Audrien Alves Andrade, Fernanda Cunha Jácome, Marcos Alexandre Nunes da Silva, Gabriela Cardoso Caldas, Ortrud Monika Barth, Debora Ferreira Barreto-Vieira 2017. Zika Virus propagation. protocols.io dx.doi.org/10.17504/protocols.io.jkackse | 2021-03-29 03:11:56 | ||||
|
Marine Purity Broth Resource Report Resource Website |
Bonnie Poulos | 10.17504/protocols.io.ekrbcv6 | VERVE Net, Sullivan Lab | Purpose: To test axenic cultures for purity. Protocol described in S. Bertillson, O. Berglund, D.M. Karl, S.W. Chisholm (2003). Elemental composition of marine Prochlorococcus and Synechococcus: Implications for the ecological stoichiometry of the sea. Limnol Oceanogr 48(5):1721-1731. | Matthew Sullivan Lab, University of Arizona, The Ohio State University | 1 | 2016 | Bonnie Poulos 2016. Marine Purity Broth. protocols.io dx.doi.org/10.17504/protocols.io.ekrbcv6 | 2021-03-29 03:11:55 | |||
|
ATP synthase activity assay (radioactive) Resource Report Resource Website |
Anika Wiegard, Christin Köbler, Katsuaki Oyama, Anja K. Dörrich, Chihiro Azai, Kazuki Terauchi, Annegret Wilde, Ilka Maria Axmann | 10.17504/protocols.io.48qgzvw | Axmann Lab, CyanoWorld | This protocol describes how to detect synthesis of [α32P]ATP from [α32P]ATP by recombinant KaiC proteins. Radioactive nucleotides are separated via thin layer chromatography using TLC PEI Cellulose F plates as stationary phase and LiCl as soluble phase. The principle of this method is based on Egli et al. (Egli M, Mori T, Pattanayek R, Xu Y, Qin X, Johnson CH.2012. Dephosphorylation of the core clock protein KaiC in the cyanobacterial KaiABC circadian oscillator proceeds via an ATP synthase mechanism. Biochemistry 51:1547-58.) | Wiegard A, Köbler C, Oyama K, Dörrich AK, Azai C, Terauchi K, Wilde A, Axmann IM, Array. Journal of Bacteriology 202(4). doi: 10.1128/JB.00478-19 | Institute for Synthetic Microbiology, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine University Duesseldorf, 40225 Duesseldorf, Germany, Institute of Biology III, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany, Graduate School of Life Sciences, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan, Institute for Microbiology and Molecular Biology, Justus-Liebig University, 35392 Giessen, Germany, College of Life Sciences, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan; Graduate School of Life Sciences, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan, College of Life Sciences, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan; Graduate School of Life Sciences, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan, Institute of Biology III, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany, Institute for Synthetic Microbiology, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine University Duesseldorf, 40225 Duesseldorf, Germany | https://doi.org/10.1128/JB.00478-19 | 1 | 2019 | Anika Wiegard, Christin Köbler, Katsuaki Oyama, Anja K. Dörrich, Chihiro Azai, Kazuki Terauchi, Annegret Wilde, Ilka Maria Axmann 2019. ATP synthase activity assay (radioactive). protocols.io dx.doi.org/10.17504/protocols.io.48qgzvw | 2021-03-29 03:11:56 | |
|
Fluorescent immunochemistry Resource Report Resource Website |
Corentin Cras-Méneur | 10.17504/protocols.io.brkvm4w6 | General fluorescent immunostaining protocol for paraffin sections | University of Michigan - Ann Arbor | 1 | 2021 | Corentin Cras-Méneur 2021. Fluorescent immunochemistry. protocols.io dx.doi.org/10.17504/protocols.io.brkvm4w6 | 2021-03-29 03:11:54 | ||||
|
Isolation of endothelial cells from umbillical vessels Resource Report Resource Website |
Thomas Mudersbach, Daniel Siuda, Karin Kohlstedt, Ingrid Fleming | 10.17504/protocols.io.ybdfsi6 | Mudersbach T, Siuda D, Kohlstedt K, Fleming I (2019) Epigenetic control of the angiotensin-converting enzyme in endothelial cells during inflammation. PLoS ONE 14(5): e0216218. doi: 10.1371/journal.pone.0216218 | Institute for Vascular Signalling, Centre for Molecular Medicine, Goethe University, Frankfurt am Main, Germany, Institute for Vascular Signalling, Centre for Molecular Medicine, Goethe University, Frankfurt am Main, Germany, Institute for Vascular Signalling, Centre for Molecular Medicine, Goethe University, Frankfurt am Main, Germany, Institute for Vascular Signalling, Centre for Molecular Medicine, Goethe University, Frankfurt am Main, Germany | https://doi.org/10.1371/journal.pone.0216218 | 1 | 2019 | Thomas Mudersbach, Daniel Siuda, Karin Kohlstedt, Ingrid Fleming 2019. Isolation of endothelial cells from umbillical vessels. protocols.io dx.doi.org/10.17504/protocols.io.ybdfsi6 | 2021-03-29 03:11:54 | |||
|
Amplification of phage genes from lysates and environmental samples Resource Report Resource Website |
Matthew Sullivan | 10.17504/protocols.io.djn4md | VERVE Net, Sullivan Lab | From Sullivan M., Lindell D., Lee J., Thompson L., Bielawski J., Chisholm S. Prevalence and Evolution of Core Photosystem II Genes in Marine Cyanobacterial Viruses and Their Hosts.PLOS Biology, 2006 4(8):e234. Please see the published manuscript for additional information. | Matthew Sullivan Lab, University of Arizona, Ohio State University | 1 | 2016 | Matthew Sullivan 2016. Amplification of phage genes from lysates and environmental samples. protocols.io dx.doi.org/10.17504/protocols.io.djn4md | 2021-03-29 03:11:57 | |||
|
scratch, don't use, delete ( the biology is unexpected and something we don't want to invest time in figuring down right now ) Resource Report Resource Website |
Darach Miller | 10.17504/protocols.io.fnnbmde | The aim of this protocol is to describe how to do a very reproducible upshift of a nitrogen-limited yeast culture to nitrogen non-limited conditions. In order to do this with enough cells to make inefficient analysis (ie RATEseq) feasible, we start in the chemostat for high-cell counts. | 2 | 2016 | Darach Miller 2016. scratch, don't use, delete ( the biology is unexpected and something we don't want to invest time in figuring down right now ). protocols.io dx.doi.org/10.17504/protocols.io.fnnbmde | 2021-03-29 03:11:57 | |||||
|
PMN- 03 Culture of Human PMN - Migration Resource Report Resource Website |
Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino | 10.17504/protocols.io.bhrmj546 | Published work using this protocol:- A Novel Standardized Cannabis sativa L. Extract and Its Constituent Cannabidiol Inhibit Human Polymorphonuclear Leukocyte Functions.Alex Mabou Tagne, Franca Marino, Massimiliano Legnaro, Alessandra Luini, Barbara Pacchetti, Marco Cosentino. Int J Mol Sci. 2019 Apr 13;20(8):1833. doi: 10.3390/ijms20081833.- β 2 -Adrenoceptors Inhibit Neutrophil Extracellular Traps in Human Polymorphonuclear Leukocytes.Franca Marino, Angela Scanzano, Laura Pulze, Monica Pinoli, Emanuela Rasini, Alessandra Luini, Raffaella Bombelli, Massimiliano Legnaro, Magda de Eguileor, Marco Cosentino . J Leukoc Biol. 2018 Sep;104(3):603-614. doi: 10.1002/JLB.3A1017-398RR. Epub 2018 Apr 18.- Adrenergic Modulation of Migration, CD11b and CD18 Expression, ROS and interleukin-8 Production by Human Polymorphonuclear Leukocytes.Angela Scanzano, Laura Schembri, Emanuela Rasini, Alessandra Luini, Jessica Dallatorre, Massimiliano Legnaro, Raffaella Bombelli, Terenzio Congiu, Marco Cosentino, Franca Marino. Inflamm Res. 2015 Feb;64(2):127-35. doi: 10.1007/s00011-014-0791-8. Epub 2015 Jan 6. | Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy), Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy), Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy), Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy), Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy), Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy), Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy) | 1 | 2020 | Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino 2020. PMN- 03 Culture of Human PMN - Migration. protocols.io dx.doi.org/10.17504/protocols.io.bhrmj546 | 2021-03-29 03:11:56 | ||||
|
Vandy - Hyperglycemic clamp Resource Report Resource Website |
Li Kang | 10.17504/protocols.io.yxefxje | Mouse Metabolic Phenotyping Centers | Summary: Mice with catheters implanted in the jugular vein (infusions) and carotid artery (sampling) are used for this procedure. The hyperglycemic clamp is used to assess insulin secretory capacity in conscious mice in response to hyperglycemia. Plasma insulin and C-Pedtide concentrations are measured at various times during the 2-hr clamp period. | Vanderbilt University | https://mmpc.org/shared/document.aspx?id=240&docType=Protocol | 1 | 2019 | Li Kang 2019. Vandy - Hyperglycemic clamp. protocols.io dx.doi.org/10.17504/protocols.io.yxefxje | 2021-03-29 03:11:55 | ||
|
Combined Single-Cell Measurement of Cytokine mRNA and Protein in Immune Cells Resource Report Resource Website |
Julian J. Freen-van Heeren, Benot P. Nicolet, Monika C. Wolkers | 10.17504/protocols.io.3t9gnr6 | A key feature of immune cells, such as T cells, is their rapid responsiveness to activation. The response rate of T cells depends on the type of signals they receive, and the signal strength. Studying the underlying mechanisms that define responsiveness, however, is confounded by the fact that immune cells do not uniformly respond to activation. Tools that measure gene products on a single cell level therefore provide additional insights in T cell biology. Here we describe Flow-FISH, a high-throughput assay that allows for the simultaneous measurement of cytokine mRNA and protein levels of the gene(s) of interest by flow cytometry. We present several possible applications of Flow-FISH in human and murine T cells that - with minor adjustments - should also be applicable for other gene products and cell types. This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology, and is currently in press. | Department of Hematopoiesis, Sanquin Research-Amsterdam UMC Landsteiner Laboratory, Amsterdam, The Netherlands, Department of Hematopoiesis, Sanquin Research-Amsterdam UMC Landsteiner Laboratory, Amsterdam, The Netherlands, Department of Hematopoiesis, Sanquin Research-Amsterdam UMC Landsteiner Laboratory, Amsterdam, The Netherlands | 1 | 2019 | Julian J. Freen-van Heeren, Benot P. Nicolet, Monika C. Wolkers 2019. Combined Single-Cell Measurement of Cytokine mRNA and Protein in Immune Cells. protocols.io dx.doi.org/10.17504/protocols.io.3t9gnr6 | 2021-03-29 03:11:55 | ||||
|
Monoamine oxidase activity in fish brain tissue Resource Report Resource Website |
Caio Maximino, Denis Broock Rosembeg | 10.17504/protocols.io.bgu9jwz6 | Fish behavior and physiology, Medicinal Plants Southeastern Pará Research Group | The protocol describes a spectrofluorophotometric method for rapid determination of monoamine oxidase (MAO) activity in zebrafish brains. The protocol is based on the transformation of kynuramine hydrobromide into 4-hydroxyquinoline. Since zebrafish possess only one MAO isoform, inhibitors of other isoforms are not necessary. | Universidade Federal do Sul e Sudeste do Pará, Universidade Federal de Santa Maria | 1 | 2020 | Caio Maximino, Denis Broock Rosembeg 2020. Monoamine oxidase activity in fish brain tissue . protocols.io dx.doi.org/10.17504/protocols.io.bgu9jwz6 | 2021-03-29 03:11:55 | |||
|
untitled protocol Resource Report Resource Website |
Elena I. Zuykova, Evgeniy P. Simonov, Nickolai A. Bochkarev, Sergey A. Abramov, Natalia G. Sheveleva, Alexey A. Kotov | 10.17504/protocols.io.s7pehmn | Here we studied the genetic polymorphism of the ribosomal 12S and protein-coding ND2 mitochondrial genes in three closely related taxa of the Daphnia (Daphnia) longispina complex, namely D. galeata Sars, D. longispina O.F. Müller and D. dentifera Forbes, and performed an analysis of phylogenetic relationships between these taxa based on concatenated sequences of two genes. | Zuykova EI, Simonov EP, Bochkarev NA, Abramov SA, Sheveleva NG, Kotov AA (2018) Contrasting phylogeographic patterns and demographic history in closely related species of Daphnia longispina group (Crustacea: Cladocera) with focus on North-Eastern Eurasia. PLoS ONE 13(11): e0207347. doi: 10.1371/journal.pone.0207347 | , , , , , A.N. Severtsob Institute of ecology and Evolution, Russian academy of Sciences, Moscow, Russia | https://doi.org/10.1371/journal.pone.0207347 | 1 | 2018 | Elena I. Zuykova, Evgeniy P. Simonov, Nickolai A. Bochkarev, Sergey A. Abramov, Natalia G. Sheveleva, Alexey A. Kotov 2018. untitled protocol. protocols.io dx.doi.org/10.17504/protocols.io.s7pehmn | 2021-03-29 03:11:57 |
Can't find your Protocol?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific protocol and you know the DOI of the protocol already, it's easier to enter a DOI to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your protocol in the search results, please help us by adding it into the system — it's easy. Create and publish your protocols at Protocols.io.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
