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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Protein LAG (SpLAG) is an immunoglobulin-binding protein that interacts with the Fc and Fab regions of many mammalian immunoglobulins. It is produced by a chemical coupling of individual proteins and them mixing it up to the appropriate protein ratio. SpLAG binds well to some avian immunoglobulin [1].References1.Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175
Proper citation: Angel Justiz-Vaillant 2020. Direct ELISA for investigating the binding of chemically-made Protein-LAG to immunoglobulins.. protocols.io dx.doi.org/10.17504/protocols.io.bj4zkqx6 Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Keyhole limpet hemocyanin (KLH) is a cooper-containing protein comprising of subunits with MW of 400 kDa. This protein is found in the hemolymph of the sea mollusk Megathura crenulata. It has the ability to enhance the host’s immune response by interacting with monocytes, T cells and macrophages. KLH has been used primarily as a carrier for vaccines and antigens [1]. It was found that chicken immunized with KLH bound peptide raised an anti-KLH immunoresponse [2]. This can be tested by a single method such as the Ouchterlony technique.Reference1. Aarntzen EH, de Vries IJ, Göertz JH, et al. Humoral anti-KLH responses in cancer patients treated with dendritic cell-based immunotherapy are dictated by different vaccination parameters.Cancer Immunol Immunother. 2012;61(11):2003-2011. doi:10.1007/s00262-012-1263-z2. Justiz Vaillant AA, Anderson MF, Smikle M, Wisdom B, Mohammed W, et al. (2013) Development of Anti HIV Gp120 and HIV Gp41 Peptide Vaccines. J Vaccines Vaccin 4: 206. doi: 10.4172/2157-7560.1000206
Proper citation: Angel Justiz-Vaillant 2020. Detection of anti- SpA antibodies in egg white tested by double immunodiffusion (Ouchterlony) technique.. protocols.io dx.doi.org/10.17504/protocols.io.bjsrknd6 Copy
Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human properdin in serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bj7zkrp6 Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant 2020. Enzyme linked immunosorbent assay for investigating the immunoglobulin-binding bacterial protein (IBBP) to avian egg yolk antibodies.. protocols.io dx.doi.org/10.17504/protocols.io.bjnzkmf6 Copy
Authors: Angel Justiz-Vaillant, Patrick E. Akpaka
Group: University of the West Indies, [email protected]
Summary: This test shows the ability of Ab-3 antibody to inhibit the growth of S. aureus in vitro. The amounts of samples tested were 18 samples from chicks fed with hyper-immune eggs and therefore have the Ab-3, and six samples from chicks fed non-hyperimmune eggs and defenetively do not have the Ab-3. Inhibition of the growth of the bacteria plated out in blood agar was observed only when the Ab-3 was added to said culture media, sugesting that the anti-anti-anti-SpA (Ab-3) bound to the S. aureus and inhibited their ability to proliferate in culture. On the other hand, bacterial growth was observed when blanks or sera of chicks fed non-hyper-immune eggs were used because these samples lacked the protective Ab-3 [1]. ReferenceJustiz Vaillant AA, Akpaka PE, Smikle M, McFarlane-Anderson N (2012) In vitro Inhibition of Staphylococcus aureus isolates by anti-antiIdiotypic antibodies to Staphylococca protein (SpA). J Vaccines Vaccin 3:127. doi:10.4172/2157-7560.1000127.
Proper citation: Angel Justiz-Vaillant, Patrick E. Akpaka 2020. Protocol for study of the in vitro effect of purified serum anti-anti-anti-SpA antibodies (Ab-3) on cultures of Staphylococcus aureus.. protocols.io dx.doi.org/10.17504/protocols.io.bjkekkte Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Enzyme-Linked Immunosorbent Assay (ELISA) was used for screening while the Western Blot was used for confirmation.
Proper citation: Angel Justiz-Vaillant 2020. Immunoblot analysis for immunodetection of HIV proteins.. protocols.io dx.doi.org/10.17504/protocols.io.bjtpknmn Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Protein LG (SpLG) is an immunoglobulin-binding protein that interacts with the Fab and Fc regions of many mammalian immunoglobulins [1].References1.Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175
Proper citation: Angel Justiz-Vaillant 2020. Direct ELISA for investigating the binding of recombinant or chemically-made Protein-LG to immunoglobulins.. protocols.io dx.doi.org/10.17504/protocols.io.bjxvkpn6 Copy
Authors: Angel Justiz-Vaillant, Norma McFarlane-Anderson
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Universal sandwich enzyme linked immunosorbent assay for investigating protein-AG (SpAG) interactions with immunoglobulins using a SpL-HRP conjugate.. protocols.io dx.doi.org/10.17504/protocols.io.bjpxkmpn Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: A previous protocol was contaminated with IL-17. This is an improved version of an ELISA for quantification of Interleukin 23. It activates the production of IL-17A, which is a pro-inflammatory cytokine, produced by a group of T helper cell known as T- helper17 lymphocytes in response to the said IL-23 [1,2].Reference1. Cho ML, Kang JW, Moon YM, Nam HJ, Jhun JY, Heo SB, Jin HT, Min SY, Ju JH, Park KS, Cho YG, Yoon CH, Park SH, Sung YC, Kim HY (May 2006). Journal of Immunology. 176 (9): 5652–61. 2. Nobee A, Justiz-Vaillant A, Akpaka PE, Poon-King P (2016) Levels of Interleukin 17 and 23 in Patients with Systemic Lupus Erythematosus (SLE) in Trinidad and Tobago. Immunochem Immunopathol 2: 115. doi: 10.4172/2469- 9756.1000115
Proper citation: Angel Justiz-Vaillant 2020. Improved protocol of ELISA for determining the serum concentration of IL-23 in humans.. protocols.io dx.doi.org/10.17504/protocols.io.bjzakp2e Copy
Authors: Angel Justiz-Vaillant, Monica F. Smikle
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant, Monica F. Smikle 2020. Universal sandwich ELISA for investigating the binding of Protein-AG (SpAG) to avian immunoglobulins using a peroxidase-labeled -anti-IgY conjugate.. protocols.io dx.doi.org/10.17504/protocols.io.bjqzkmx6 Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant 2020. Protein isolation from the extract of coelomic fluid of the Lumbricus terrestris.. protocols.io dx.doi.org/10.17504/protocols.io.bjsaknae Copy
Authors: Angel Justiz-Vaillant1, Arvind Kurhade1, Chandrashekhar Unakal1, Patrick E. Akpaka1, Sade Aisha Folashade John1
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant1, Arvind Kurhade1, Chandrashekhar Unakal1, Patrick E. Akpaka1, Sade Aisha Folashade John1 2020. Isolation of Escherichia coli pathogens from oysters. protocols.io dx.doi.org/10.17504/protocols.io.bj6ikrce Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Chemical synthesis facilitates the generation of peptides which are very difficult to express in bacteria, peptide/protein backbone modification, the incorporation of unnatural amino acids, and the production or synthesis of D-proteins. The C-terminal cysteine can be added to the amino acidic sequences of HIV peptides (fragment 579-601 of the HIV-gp41 and fragments 308-331 or 421-438 of the HIV-gp120). These peptide fragments were dimerized by cysteine oxidation with dimethyl-sulfoxide [1] to faciliutate their conjugation to keyhole limpet hemocyanin that acts as a carrier protein. Reference:1. Tam JP, Wu CR, Liu w, Zhang JW (1991) Disulfide bond formation in peptides by dimethyl sulfoxide. Scope and applications. J Am Chem Soc 113: 66576662.
Proper citation: Angel Justiz-Vaillant 2020. Conjugation of peptide fragment 579-601 of HIV-gp41 or fragments 308-331 or 421-438 of the HIV-gp120 with keyhole limpet hemocyanin (KLH).. protocols.io dx.doi.org/10.17504/protocols.io.bjhukj6w Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Macrophages mostly make IL-18, which can be produced by hepatocytes and keratinocytes. Its principal target is a co-factor in Th1 cell induction. It causes interferon gamma production and enhances NK cell activity. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-18 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj3hkqj6 Copy
Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Summary: IgG is a monomer with an approximate molecular weight of 146 Kd and a serum concentration of 9.0 mg/mL. It is synthesized mostly in the secondary immune response to pathogens. IgG can activate the classical pathway of the complement system, and it also is highly protective. The four subclasses of IgG include IgG1, IgG2, IgG3, and IgG4. IgG crosses the placentae, protecting the neonate from infectious diseases. [1]
Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human immunoglobulin G (IgG) in serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bj7skrne Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Enzyme-linked immunosorbent assay (ELISA) for studying the presence of anti-Salmonella antibody in layer hens was a reproducible and feseable test used to meassure IgY development after vaccination.
Proper citation: Angel Justiz-Vaillant 2020. Enzyme-linked immunosorbent assay (ELISA) for studying the presence of anti-Salmonella antibody in layer hen's egg yolks.. protocols.io dx.doi.org/10.17504/protocols.io.bj54kq8w Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Regulatory B cells mainly secrete IL-35. One of the primary functions of this interleukin is its involvement in lymphocyte differentiation. It exhibits an immune-suppressive effect. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-35 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj37kqrn Copy
Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human C3 in serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bj72krqe Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Il-38 acts on T cells and inhibits the synthesis of IL-17 and IL-22. The placenta, tonsil's B lymphocytes, spleen, skin, and thymus widely express IL-38. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-38 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj4dkqs6 Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Immune cells and activated epithelial cells secrete IL-20. It acts on epithelial cells. It plays a vital role in the cellular communication between epithelial cells and the immune system under inflammatory conditions.[1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-20 in human serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bkewktfe Copy
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