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Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Reaction Mixture for M0371. protocols.io https://dx.doi.org/10.17504/protocols.io.cgutwv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocol is for dephosphorylation of 5´-ends of DNA using rSAP in restriction enzyme reaction (M0371)
Proper citation: New England Biolabs 2015. Dephosphorylation using rSAP in Restriction Enzyme Reaction(M0371). protocols.io https://dx.doi.org/10.17504/protocols.io.cgvtw5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for immunoprecipitation using Protein A/G Magnetic Beads.
Proper citation: New England Biolabs 2015. Immunoprecipitation using Protein A/G Magnetic Beads. protocols.io https://dx.doi.org/10.17504/protocols.io.crav2d Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Q5 PCR Mixture for M0302. protocols.io https://dx.doi.org/10.17504/protocols.io.cqqvvv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This PCR Protocol is for Taq DNA Polymerase with Standard Taq Buffer (M0273)
Proper citation: New England Biolabs 2015. PCR with Taq DNA Polymerase (M0273). protocols.io https://dx.doi.org/10.17504/protocols.io.ch7t9m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Please see the NEB website for more information.
Proper citation: New England Biolabs 2015. Ligation Protocol WITH T4 DNA Ligase (M0202). protocols.io https://dx.doi.org/10.17504/protocols.io.cdks4v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010).Quickly and easily purify up to 100 µg of high-quality total RNA from multiple sample types – all with one kit!For use with blood, cells and tissuesAlso works with tough to lyse samples (bacteria, yeast, plant)Effectively purifies total RNA of all sizes, including small RNAs >20 ntEfficient genomic DNA removal (column and DNase I-based)Contains Proteinase K for processing of tissues and blood samplesIncludes RNA Protection Reagent for sample preservationExcellent valueKit components available separately
Proper citation: New England Biolabs 2018. Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010). protocols.io https://dx.doi.org/10.17504/protocols.io.p26dqhe Copy
Authors: Menna Teffera, New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II End Repair/dA-Tailing Module is optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5 ́ phosphorylated, 3 ́ dA-tailed ends. This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext™ Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction.
This module is also compatible with some Oxford Nanopore MinION™workflows.This module is designed for use with NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600 or #E7535), NEBNext Ultra II Ligation Module (NEB #E7595), and NEBNext Ultra II Q5 Master Mix (NEB #M0544). Kits that include reagents for every step in the Ultra II DNA library construction workflow are also available (NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) and NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB #E7103).
Proper citation: Menna Teffera, New England Biolabs 2019. NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB #E7546). protocols.io https://dx.doi.org/10.17504/protocols.io.4nngvde Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II FS DNA Module contains the enzymes and buffers required to convert a broad range of input amounts of intact DNA into fragmented DNA with 5´ phosphorylated 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595) and with the NEBNext Ultra II Q5 Master Mix (NEB #M0544) if amplification is required. The fast, user-friendly workflow has minimal hands on time.Note: The Ultra II FS Module is not compatible with bisulfite conversion workflows
Proper citation: New England Biolabs 2018. NEBNext Ultra II FS DNA Module E7810. protocols.io https://dx.doi.org/10.17504/protocols.io.n34dgqw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. T4 PNK Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.cpevjd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. A-Tailing with Taq Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.crxv7m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. M0348 Digestion Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.cr5v85 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Please see the NEB website for more information.
Proper citation: New England Biolabs 2017. Ligation Protocol with T4 DNA Ligase (M0202). protocols.io https://dx.doi.org/10.17504/protocols.io.irscd6e Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.Purification of ≥10 mg His-tagged protein per 1 ml of resinIntended for use in gravity or pressure flow columns, and batch purificationsHigh specific binding of His-tagged proteins yielding purities of >95%Strong nickel ion binding provides excellent resistance to EDTA and reducing agents. Compatible with commercially available detergent-based cell lysis reagentsIsolation and purification of His-tagged fusion proteins under native or denaturing conditions
Proper citation: New England Biolabs 2020. NEBExpress Ni Resin Gravity Flow Typical Protocol (NEB #S1428). protocols.io https://dx.doi.org/10.17504/protocols.io.bfd7ji9n Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2014. Mixture for M0492 Q5 2X Master Mix. protocols.io https://dx.doi.org/10.17504/protocols.io.cijucm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II FS DNA Module contains the enzymes and buffers required to convert a broad range of input amounts of intact DNA into fragmented DNA with 5´ phosphorylated 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595) and with the NEBNext Ultra II Q5 Master Mix (NEB #M0544) if amplification is required. The fast, user-friendly workflow has minimal hands on time.
Proper citation: New England Biolabs 2020. E7805 NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® Protocol for use with Inputs ≤ 100 ng. protocols.io https://dx.doi.org/10.17504/protocols.io.k8tczwn Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for a large-scale Protein Expression Using BL21(DE3) Competent E. coli cells(C2527).
Proper citation: New England Biolabs 2014. Protein Expression Using BL21(DE3), Large Scale (C2527). protocols.io https://dx.doi.org/10.17504/protocols.io.cjrum5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for Protein Expression Using BL21(DE3) Competent E. coli (C2527). For large scale, please follow this protocol variant.
Proper citation: New England Biolabs 2014. Protein Expression Using BL21(DE3) (C2527). protocols.io https://dx.doi.org/10.17504/protocols.io.cjqumv Copy
Authors: New England Biolabs
Group: Grupo de Estudos de Leveduras Cervejeiras
Proper citation: New England Biolabs 2018. SOB Media. protocols.io https://dx.doi.org/10.17504/protocols.io.pnfdmbn Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. E1202 Ligation Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.cp6vrd Copy
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