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Authors: Angel Justiz-Vaillant
Group: Carbon
Summary: This ELISA was used to study the interactions between protein-LAG (PLAG) and protein-LG (SpLG) with different immunoglobulin preparations from mammalian species.

Proper citation: Angel Justiz-Vaillant 2021. Chimeric Protein-LAG and Protein-LG sandwich ELISA. protocols.io https://dx.doi.org/10.17504/protocols.io.btbdnii6 Copy   

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Authors: Angel Justiz-Vaillant, Monica F. Smikle
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant, Monica F. Smikle 2020. Sandwich ELISA for investigating the binding of Protein-LG (SpLG) to avian immunoglobulins using anti-IgY-peroxidase as conjugate.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjq4kmyw Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Monocytes and fibroblasts make IL-8. Its principal targets are neutrophils, basophils, mast cells, macrophages, and keratinocytes. It causes neutrophil chemotaxis, angiogenesis, superoxide release, and granule release.[1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-8 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj27kqhn Copy   

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Authors: Angel Justiz-Vaillant, Norma McFarlane-Anderson
Group: University of the West Indies, [email protected]
Summary: This SpL ELISA can be used to detect specific antibodies in various animal species including human, mouse, rat, dog, rabbit, chicken, monkey, pig and hamster [1].1. De Chateau M, Nilson BH, Erntell M, Myhre E, Magnusson CG, Akerstrom B et al. On the interaction between protein L and immunoglobulins of various mammalian species. Scand J Immunol 1993; 37: 399−405

Proper citation: Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Enzyme linked immunosorbent assay for investigating the binding of protein-LA (SpLA) to immunoglobulins. . protocols.io https://dx.doi.org/10.17504/protocols.io.bjpjkmkn Copy   

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Authors: Angel Justiz-Vaillant
Group: Carbon
Summary: This ELISA was used to study the interactions between protein-LAG (PLAG) and protein-AG (SpAG) with different immunoglobulin preparations from mammalian and avian species.

Proper citation: Angel Justiz-Vaillant 2021. Chimeric Protein-LAG and Protein-AG sandwich ELISA  . protocols.io https://dx.doi.org/10.17504/protocols.io.btbfnijn Copy   

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Authors: Angel Justiz-Vaillant
Group: Carbon
Summary: This ELISA is used to study the interaction between protein-AG (SpAG) and protein-LAG (PLAG) with different immunoglobulin preparations from mammalian and avian species.

Proper citation: Angel Justiz-Vaillant 2021. Chimeric Protein-AG and Protein-LAG sandwich ELISA. protocols.io https://dx.doi.org/10.17504/protocols.io.bta9nih6 Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. They have both paracrine and autocrine function. Interleukins are also used in animal studies to investigate aspect related to clinical medicine.T cells and stem cells make IL-3. It functions as a multilineage colony-stimulating factor [1]CD4+T cells (Th2) synthesize IL-4, and it acts on both B and T cells. It is a B-cell growth factor and causes IgE and IgG1 isotype selection. It causes Th2 differentiation and proliferation, and it inhibits IFN gamma-mediated activation on macrophages. It promotes mast cell proliferation in vivo. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-4 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj24kqgw Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Peroxidase-labeled-protein-LAG-anti-IgY conjugate is chemically made. It has unique binding properties. It binds to both avian and mammalian immunoglobulins. It can be used as a reagent in ELISA, Western blotting and dot blot for immunodetection of immunoglobulins and antigens. It may be used to make the immunodiagnosis of infectious diseases involving laboratory, wild, zoo, and farm animals [1].References1.Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175

Proper citation: Angel Justiz-Vaillant 2020. Direct ELISA for investigating the binding of chemically-made Protein-LAG-anti-IgY-peroxidase  to both avian and mammalian immunoglobulins.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjxzkpp6 Copy   

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Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. [1]The immunoregulatory cytokine IL-41 (also known as meteorin-like protein) is expressed at high levels in the synovium of patients with psoriatic arthritis (PsA).[2]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.2.Onuora S. Novel cytokine, IL-41, linked with PsA.Nat Rev Rheumatol. 2019;15(11):636. doi:10.1038/s41584-019-0314-7

Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of granulocyte macrophage-colony stimulating factor (GM-CSF) in human serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bktbkwin Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Immunoglobulin Y (IgY) is the mayormolecule present in the avian egg yolk. This protein functions as antibody and fulfils important functions in the protection of the embryo against challenging stimuli [1]. IgY has been isolated from the egg yolk of many birds [1]. These antibodies are important therapeutically, but also they have been used as immunological tool in many immunodiagnostic assays.Much time IgY concentration is calculated from the water soluble fraction (WSF), which means that there is not need of treating the preparation with alcohol to separate the protein pellet. Reference1. Justiz Vaillant AA, Ramirez N, Cadiz A, Ferrer B, Akpaka P, et al. (2013) Separation and Reactivity of Avian Immunoglobulin Y. J Chromat Separation Techniq 4: 173. doi:10.4172/2157-7064.1000173

Proper citation: Angel Justiz-Vaillant 2020. Polson method for IgY isolation with modification. . protocols.io https://dx.doi.org/10.17504/protocols.io.bjiykkfw Copy   

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Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of Vascular endothelial growth factor A (VEGFA) in cell culture supernatant, human serum or plasma. protocols.io https://dx.doi.org/10.17504/protocols.io.bkttkwnn Copy   

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Authors: Angel Justiz-Vaillant, Norma McFarlane-Anderson
Group: University of the West Indies, [email protected]
Summary: This SpLAG-anti-IgY ELISA can be used to detect specific antibodies in various animal species including human, goat, donkey, mouse, rat, dog, cow, horse, ostrich, duck, pigeon, bantam hen, rabbit, chicken, monkey, pig, hamster, and many birds, wild and zoo animals species [1].1. Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175

Proper citation: Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Enzyme linked immunosorbent assay for investigating the binding of chemically prepared protein-LAG-anti-IgY (SpLAG-anti-IgY) to avian and mammalian immunoglobulins. . protocols.io https://dx.doi.org/10.17504/protocols.io.bjpskmne Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant 2020. Determination of IgA concentration by the Mancini test.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj93kr8n Copy   

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Authors: Angel Justiz-Vaillant, Norma McFarlane-Anderson
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Universal sandwich enzyme linked immunosorbent assay for investigating immunoglobulin-binding protein (IBP) interactions using a conjugate SpAG-HRP.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjptkmnn Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. IL-40 is produced in the bone marrow, fetal liver, and by activated B cells. IL-40 plays a vital role in the development of humoral immune responses. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-40 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj4fkqtn Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant 2020. Feeding of cats with hyper-immune eggs: an oral immunization protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bjnnkmde Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of macrophage-colony stimulating factor (M-CSF) in human serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bks7kwhn Copy   

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Authors: Angel Justiz-Vaillant
Summary: This ELISA was used to study the interactions between Staphylococcal protein-A (SpA) and protein-LG (SpLG) with different immunoglobulin preparations from mammalian and avian species.

Proper citation: Angel Justiz-Vaillant 2021. Protein-A and Protein-LG Sandwich ELISA. protocols.io https://dx.doi.org/10.17504/protocols.io.bsu9nez6 Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. IL-32 is a pro-inflammatory molecule. Natural killer cells and monocytes mainly produce it. IL-32 induces the synthesis of various cytokines including IL-6, and IL-1beta. It inhibits IL-15 production. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-32 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj35kqq6 Copy   

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Authors: Angel Justiz-Vaillant, Monica F. Smikle
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant, Monica F. Smikle 2020. Universal sandwich ELISA for investigating the binding of Protein L to avian immunoglobulins using anti-IgY-peroxidase as conjugate.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjqykmxw Copy   

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