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Protocol for Lucifersae Plasmid Prep Resource Report Resource Website |
Fatma Gomaa, Zhuhong Li, Roberto Docampo, Peter Girguis, Virginia Edgcomb | DOI:10.17504/protocols.io.niadcae | Harvard University, University of Georgia, University of Georgia, Harvard University, Woods Hole Oceanographic Institution | 1 | 2018 | Fatma Gomaa, Zhuhong Li, Roberto Docampo, Peter Girguis, Virginia Edgcomb 2018. Protocol for Lucifersae Plasmid Prep. protocols.io https://dx.doi.org/10.17504/protocols.io.niadcae | 2021-04-15 09:14:24 | |||||
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Operating of the Torion® T-9 portable gas chromatograph-mass spectrometer (PerkinElmer) for the analysis of animal scent samples Resource Report Resource Website |
Alice C. Poirier, John S. Waterhouse, Mrinalini Watsa, Gideon A. Erkenswick, Laís A. A. Moreira, Jia Tang, Jacob C. Dunn, Amanda D. Melin, Andrew C. Smith | DOI:10.17504/protocols.io.brpqm5mw | Chemosignals are mediators of social interactions in mammals, providing con- and hetero-specifics with information on fixed (e.g. species, sex, group and individual identity) and variable (e.g. social, reproductive and health status) features of the signaler. Yet methodological difficulties of recording and quantifying odor signals, especially in field conditions, have hampered studies of natural systems. We present the first use of the Torion® portable gas chromatography-mass spectrometry (GC-MS) instrument for in situ chemical analysis of primate scents. We collected and analyzed swab samples from the scent-glands and skin from 13 groups (57 individuals) of two sympatric species of wild emperor tamarins, Saguinus imperator, and Weddell’s saddleback tamarins, Leontocebus weddelli (Callitrichidae). In total, 11 compounds of interest (i.e. probably derived from the animals) could be detected in the samples, with 31 of 215 samples containing at least one compound of interest. The composition of these 31 samples varied systematically with species, group, sex and breeding status. Moreover, we tentatively identified seven of the compounds of interest as methyl hexanoate, benzaldehyde, ethyl hexanoate, acetophenone, a branched C15 alkane, 4-methoxybenzaldehyde and hexadecan-1-ol. As the field of primate semiochemistry continues to grow, we believe that portable GC-MS instruments have the potential to help make progress in the study of primate chemosignaling in field conditions, despite limitations that we encountered. We further provide recommendations for future use of the Torion® portable GC-MS for in situ analyses. | Department of Anthropology and Archaeology, University of Calgary, Calgary, Canada; School of Life Sciences, Anglia Ruskin University, Cambridge, United Kingdom, School of Life Sciences, Anglia Ruskin University, Cambridge, United Kingdom, Field Projects International, Saint Louis, Missouri, USA; Population Sustainability, Institute for Conservation Research, San Diego Zoo Global, San Diego, USA, Field Projects International, Saint Louis, Missouri, USA; Department of Biology, University of Missouri St. Louis, Saint Louis, Missouri, USA, Department of Anthropology and Archaeology, University of Calgary, Calgary, Canada, Department of Anthropology and Archaeology, University of Calgary, Calgary, Canada, School of Life Sciences, Anglia Ruskin University, Cambridge, United Kingdom; Biological Anthropology, Department of Archaeology, University of Cambridge, United Kingdom; Department of Cognitive Biology, University of Vienna, Austria, Department of Anthropology and Archaeology, University of Calgary, Calgary, Canada; Department of Medical Genetics and Alberta Children’s Hospital Research Institute, Cumming School of Medicine, University of Calgary, Calgary, Canada, School of Life Sciences, Anglia Ruskin University, Cambridge, United Kingdom | 2 | 2021 | Alice C. Poirier, John S. Waterhouse, Mrinalini Watsa, Gideon A. Erkenswick, Laís A. A. Moreira, Jia Tang, Jacob C. Dunn, Amanda D. Melin, Andrew C. Smith 2021. Operating of the Torion® T-9 portable gas chromatograph-mass spectrometer (PerkinElmer) for the analysis of animal scent samples . protocols.io https://dx.doi.org/10.17504/protocols.io.brpqm5mw | 2021-04-15 09:14:25 | ||||
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Tris Buffered Saline (TNT) Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bgncjvaw | BICCN, Allen Institute for Brain Science | This protocol is used to prepare Tris Buffered Saline (TNT). 10X TNT is the stock solution used to prepare 1X TNT, which equilibrates tissue and maintains pH in physiological range.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. Tris Buffered Saline (TNT). protocols.io https://dx.doi.org/10.17504/protocols.io.bgncjvaw | 2021-04-15 09:14:30 | |||
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U Mass - Cytokines Panel I - multiplex Resource Report Resource Website |
Jason Kim | DOI:10.17504/protocols.io.xvnfn5e | Mouse Metabolic Phenotyping Centers | Summary: This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity. Cytokines Panel I include IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KC (IL-8 homologue), IL-9, IL-10 (interleukin-10), IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, TNFα (tumor necrosis factor alpha), IFNγ (interferon gamma), IP-10 (interferon gamma-induced protein 10; CXCL-10), Eotaxin (CCL-11), G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte macrophage colony stimulating factor), LIF (leukemia inhibitory factor), LIX (LPS- induced CXC chemokine), MCP-1 (monocyte chemotactic protein-1; CCL-2), M-CSF (macrophage colony stimulating factor), MIG (monokine induced by gamma interferon; CXCL- 9), MIP-1α (macrophage inflammatory protein alpha; CCL-3), MIP-1 (macrophage inflammatory protein beta; CCL-4), and MIP-2/RANTES (macrophage inflammatory protein 2- alpha; CXCL-2). A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=190&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Cytokines Panel I - multiplex. protocols.io https://dx.doi.org/10.17504/protocols.io.xvnfn5e | 2021-04-15 09:14:25 | ||
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PCR and cloning Resource Report Resource Website |
Norfitriah Mohamed Sohaimi, Mohd Hair Bejo, Abdul Rahman Omar, Aini Ideris, Nurulfiza Mat Isa | DOI:10.17504/protocols.io.7zfhp3n | Sohaimi NM, Bejo MH, Omar AR, Ideris A, Isa NM (2019) Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens. PLoS ONE 14(12): e0225863. doi: 10.1371/journal.pone.0225863 | Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia., Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia., Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia., Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia., Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. | https://doi.org/10.1371/journal.pone.0225863 | 1 | 2019 | Norfitriah Mohamed Sohaimi, Mohd Hair Bejo, Abdul Rahman Omar, Aini Ideris, Nurulfiza Mat Isa 2019. PCR and cloning. protocols.io https://dx.doi.org/10.17504/protocols.io.7zfhp3n | 2021-04-15 09:14:25 | |||
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Script R14: CARD Antibiotic Resistance Analysis Resource Report Resource Website |
HANNIGAN GD, GRICE EA, ET AL. | DOI:10.17504/protocols.io.ejnbcme | VERVE Net, Club Grice | This protocol outlines our analysis of the potential antibiotic resistance genes found within the skin virome. We start by visualizing the relative abundances of the top 10 antibiotic resistance categories (according to the CARD). We then quantify the number of unique antibiotic resistance gene categories annotated in the samples at each skin site, and also look at the percent CARD annotated ORFs of the overall ORFs present in each sample at each skin site. | Kindler L, Stoliartchouk A, Teytelman L, Hurwitz BL, Method-centered digital communities on protocols.io for fast-paced scientific innovation. F1000Research doi: 10.12688/f1000research.9453.2 | DEPARTMENT OF DERMATOLOGY UNIVERSITY OF PENNSYLVANIA, DEPARTMENT OF DERMATOLOGY UNIVERSITY OF PENNSYLVANIA, DEPARTMENT OF DERMATOLOGY UNIVERSITY OF PENNSYLVANIA | http://mbio.asm.org/content/6/5/e01578-15.full | 1 | 2016 | HANNIGAN GD, GRICE EA, ET AL. 2016. Script R14: CARD Antibiotic Resistance Analysis. protocols.io https://dx.doi.org/10.17504/protocols.io.ejnbcme | 2021-04-15 09:14:25 | |
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Enzymatic PCR Cleanup Protocol (NEB #M0525) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.7r4hm8w | New England Biolabs (NEB) | Rapid and irreversible heat inactivation eliminates unwanted activityImproved storage stability versus native enzymeFaster reaction setup (no supplemental additives like zinc required) and shorter incubation timeFlexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)Less enzyme required (high specific activity), resulting in a lower cost per reactionNo need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysisRecombinant for purity, consistency and value | New England Biolabs | https://neb.com/protocols/2019/06/04/enzymatic-pcr-cleanup-protocol-neb-m0525 | 1 | 2020 | New England Biolabs 2020. Enzymatic PCR Cleanup Protocol (NEB #M0525). protocols.io https://dx.doi.org/10.17504/protocols.io.7r4hm8w | 2021-04-15 09:14:30 | ||
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U Cinn - NEFA Concentration Resource Report Resource Website |
Patrick Tso, Dana Lee | DOI:10.17504/protocols.io.xmnfk5e | Mouse Metabolic Phenotyping Centers | Quantitative determinations of non-esterified fatty acids in plasma/serum/lymph will be made using the NEFA-HR enzymatic colorimetric method assay. | University of Cincinnati, University of Cincinnati | https://mmpc.org/shared/document.aspx?id=196&docType=Protocol | 1 | 2019 | Patrick Tso, Dana Lee 2019. U Cinn - NEFA Concentration. protocols.io https://dx.doi.org/10.17504/protocols.io.xmnfk5e | 2021-04-15 09:14:27 | ||
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MojoSort™ Human CD14 Nanobeads Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7ahhib6 | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-cd14-nanobeads-column-protocol/4684/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human CD14 Nanobeads Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7ahhib6 | 2021-04-15 09:14:27 | ||
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BiTE® bystander T cell-dependent cellular cytotoxicity (TDCC) assay Resource Report Resource Website |
Sandra L. Ross, Marika Sherman, Patricia L. McElroy, Julie A. Lofgren, Gordon Moody, Patrick A. Baeuerle, Angela Coxon, Tara Arvedson | DOI:10.17504/protocols.io.hwfb7bn | Protocol for detecting bystander cytotoxicity in mixed cultures of target-negative and target-positive cells treated with BiTE® antibody constructs. Two methods and one modification are described. | Ross SL, Sherman M, McElroy PL, Lofgren JA, Moody G, Baeuerle PA, Coxon A, Arvedson T, Bispecific T cell engager (BiTE) antibody constructs can mediate bystander tumor cell killing. PLoS ONE 12(8). doi: 10.1371/journal.pone.0183390 | Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc. | https://doi.org/10.1371/journal.pone.0183390 | 1 | 2017 | Sandra L. Ross, Marika Sherman, Patricia L. McElroy, Julie A. Lofgren, Gordon Moody, Patrick A. Baeuerle, Angela Coxon, Tara Arvedson 2017. BiTE® bystander T cell-dependent cellular cytotoxicity (TDCC) assay. protocols.io https://dx.doi.org/10.17504/protocols.io.hwfb7bn | 2021-04-15 09:14:24 | ||
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Single cell RNA sequencing library preparation (2-level sci-RNA-seq) Resource Report Resource Website |
Sanjay Srivatsan, Junyue Cao | University of Washington, University of Washington | 1 | 2021 | Sanjay Srivatsan, Junyue Cao 2021. Single cell RNA sequencing library preparation (2-level sci-RNA-seq). protocols.io https:// | 2021-04-15 09:14:27 | ||||||
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U Mass - Transverse Aortic Constriction Resource Report Resource Website |
Mark Kelly, Timothy P. Fitzgibbons | DOI:10.17504/protocols.io.56ig9ce | Mouse Metabolic Phenotyping Centers | Summary: This is a Mouse model of Transverse aortic constriction (TAC). A commonly used experimental model for pressure overload-induced cardiac hypertrophy and heart failure. Initially leading to compensated hypertrophy of the heart, which often is associated with a temporary enhancement of cardiac contractility. Resulting in cardiac dilatation and heart failure. | University of Massachusetts Medical School, University of Massachusetts Medical School | https://mmpc.org/shared/document.aspx?id=326&docType=Protocol | 2 | 2019 | Mark Kelly, Timothy P. Fitzgibbons 2019. U Mass - Transverse Aortic Constriction. protocols.io https://dx.doi.org/10.17504/protocols.io.56ig9ce | 2021-04-15 09:14:24 | ||
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Improved protocol of ELISA for determining the serum concentration of IL-23 in humans. Resource Report Resource Website |
Angel Justiz-Vaillant | DOI:10.17504/protocols.io.bjzakp2e | University of the West Indies, [email protected] | A previous protocol was contaminated with IL-17. This is an improved version of an ELISA for quantification of Interleukin 23. It activates the production of IL-17A, which is a pro-inflammatory cytokine, produced by a group of T helper cell known as T- helper17 lymphocytes in response to the said IL-23 [1,2].Reference1. Cho ML, Kang JW, Moon YM, Nam HJ, Jhun JY, Heo SB, Jin HT, Min SY, Ju JH, Park KS, Cho YG, Yoon CH, Park SH, Sung YC, Kim HY (May 2006). Journal of Immunology. 176 (9): 5652–61. 2. Nobee A, Justiz-Vaillant A, Akpaka PE, Poon-King P (2016) Levels of Interleukin 17 and 23 in Patients with Systemic Lupus Erythematosus (SLE) in Trinidad and Tobago. Immunochem Immunopathol 2: 115. doi: 10.4172/2469- 9756.1000115 | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Improved protocol of ELISA for determining the serum concentration of IL-23 in humans.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjzakp2e | 2021-04-15 09:14:23 | |||
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Yale - Tissue Glycogen Resource Report Resource Website |
John Stack, Gary Cline | DOI:10.17504/protocols.io.y39fyr6 | Mouse Metabolic Phenotyping Centers | Procedure for determining the glycogen content within liver and muscle tissue. | Yale University, Yale University | https://mmpc.org/shared/document.aspx?id=201&docType=Protocol | 1 | 2019 | John Stack, Gary Cline 2019. Yale - Tissue Glycogen. protocols.io https://dx.doi.org/10.17504/protocols.io.y39fyr6 | 2021-04-15 09:14:27 | ||
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Identifying barriers and solutions concerning the recruitment and retention of elderly people in clinical research: Protocol for a Systematic Review Resource Report Resource Website |
Noah Forsat, Andriko Palmowski, Yannick Palmowski, Maarten Boers, Frank Buttgereit | DOI:10.17504/protocols.io.4f6gtre | Importance Elderly patients are underrepresented in clinical trials. Objective To identify barriers and strategies concerning recruitment and retention of elderly patients in clinical trials. Data Source MEDLINE (via PubMed), Cochrane Central Register of Controlled Trials (via Cochrane Library), Embase (via Ovid)Eligibility Publications stemming from original research that report on barriers of or strategies for better recruitment or retention of elderly people. Outcomes Barriers and strategies concerning recruitment and retention of elderly people in clinical trials.Critical Appraisal This study is about identifying barriers and solutions concerning recruitment and retention of elderly patients in clinical trials. Typical endpoints requiring a risk of bias appraisal such as ones that concern efficacy or safety will not be assessed, consequently, there will be no risk of bias assessments. Furthermore, a very heterogeneous set of studies will be included for which there exists no single risk of bias-assessment tool. | Department of Rheumatology and Clinical Immunology, Charité – University Medicine Berlin, Germany, Department of Rheumatology and Clinical Immunology, Charité – University Medicine Berlin, Germany, Center for Musculoskeletal Surgery, Charité – University Medicine Berlin, Germany, Department of Epidemiology and Biostatistics, and Amsterdam Rheumatology and Immunology Center, Amsterdam UMC, Vrije Universiteit Amsterdam, The Netherlands , Department of Rheumatology and Clinical Immunology, Charité – University Medicine Berlin, Germany | 1 | 2019 | Noah Forsat, Andriko Palmowski, Yannick Palmowski, Maarten Boers, Frank Buttgereit 2019. Identifying barriers and solutions concerning the recruitment and retention of elderly people in clinical research: Protocol for a Systematic Review. protocols.io https://dx.doi.org/10.17504/protocols.io.4f6gtre | 2021-04-15 09:14:23 | ||||
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Criminal Justice Policy Document Analysis . Version 2 Resource Report Resource Website |
Drew Gitomer | DOI:10.17504/protocols.io.zhsf36e | This is an adaptation of Yanovitzky & Weber - because we were interested adapting Yanovitzky and Weber's tool for criminal justice reform. All of the policymaking process stuff was kept in, but we modified the list of policy actors, and policy focus, and Appendix C. We also did our coding in NVIvo so removed Appendix D in the oritinal protocol | Rutgers Graduate School of Ed | 2 | 2019 | Drew Gitomer 2019. Criminal Justice Policy Document Analysis . Version 2. protocols.io https://dx.doi.org/10.17504/protocols.io.zhsf36e | 2021-04-15 09:14:27 | ||||
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MojoSort™ Nanobeads Column Protocol - 2 Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.69ehh3e | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-nanobeads-column-protocol-2/4594/ | 2 | 2019 | Sam Li 2019. MojoSort™ Nanobeads Column Protocol - 2. protocols.io https://dx.doi.org/10.17504/protocols.io.69ehh3e | 2021-04-15 09:14:24 | ||
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In vitro pmel-1 T cell-mediated cytotoxicity assay with CytoTox-ONE Homogenous Membrane Integrity Assay (Promega) Resource Report Resource Website |
Elinor Gottschalk, Bulent Arman Aksoy, Pinar Aksoy, Jeff Hammerbacher | DOI:10.17504/protocols.io.6j4hcqw | Hammer Lab | A plate-based assay to estimate the cytotoxic activity of pmel-1 T cells against target cancer cells. The CytoTox-ONE Homogeneous Membrane Integrity Assay from Promega (Madison, WI) is a lactate dehydrogenase (LDH) release-based assay. Dying cells have damaged cell membranes causing cytosolic components, such as LDH, to leak into the culture media. The CytoTox-ONE assay uses LDH in the culture media to convert resazurin to fluorescent resofurin. The resulting fluorescent signal is proportional to the number of non-viable cells in a sample. One advantage to using this assay for T cell-mediated cytotoxicity is that the cancer cells do not need to be removed from the wells to measure cell death, which is especially convenient with adherent cancer cell types that would require trypsinization. However, we have found that the assay produces noisy data and a set of control wells with T cells alone is required to subtract background, overall increasing the number of replicates that are necessary. We had consistent results when culturing the pmel-1 T cells with hgp100 peptide-pulsed MC38 colon cancer cells. But we had inconsistent results using pmel-1 T cells and their natural target, B16-F10 melanoma cells. This setup could be adapted for T cells other than pmel-1 and their target cells. The ratio of T cells to cancer cells and the duration of co-culture would have to be optimized. | Medical University of South Carolina, Medical University of South Carolina, Medical University of South Carolina, Medical University of South Carolina | 1 | 2019 | Elinor Gottschalk, Bulent Arman Aksoy, Pinar Aksoy, Jeff Hammerbacher 2019. In vitro pmel-1 T cell-mediated cytotoxicity assay with CytoTox-ONE Homogenous Membrane Integrity Assay (Promega). protocols.io https://dx.doi.org/10.17504/protocols.io.6j4hcqw | 2021-04-15 09:14:24 | |||
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sciMAP-ATAC Resource Report Resource Website |
Andrew Adey, Casey Thornton | DOI:10.17504/protocols.io.brhxm37n | High-throughput single cell genomic assays resolve the heterogeneity of cell states in complex tissues, however, the spatial orientation within the network of interconnected cells is lost. As cell localization is a necessary dimension in understanding complex tissues and disease states, we present a tool for highly scalable spatially-resolved single cell profiling of chromatin state. We use high density multiregional sampling to perform single-cell combinatorial indexing on Microbiopsies Assigned to Positions for the Assay for Transposase Accessible Chromatin (sciMAP-ATAC) to produce single-cell data of equivalent quality to non-spatial single-cell ATAC-seq. | Oregon Health & Science University, Oregon Health & Science University | 2 | 2021 | Andrew Adey, Casey Thornton 2021. sciMAP-ATAC. protocols.io https://dx.doi.org/10.17504/protocols.io.brhxm37n | 2021-04-15 09:14:30 | ||||
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Dephosphorylation using CIP in Restriction Enzyme Reaction (M0290) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cdhs35 | New England Biolabs (NEB) | Protocol for Dephosphorylation of 5´-ends of DNA using CIP in Restriction Enzyme Reaction. Uses the Calf Intestinal Alkaline Phosphatase (CIP - M0290) | New England Biolabs | https://www.neb.com/protocols/1/01/01/protocol-for-dephosphorylating-with-cip | 1 | 2014 | New England Biolabs 2014. Dephosphorylation using CIP in Restriction Enzyme Reaction (M0290). protocols.io https://dx.doi.org/10.17504/protocols.io.cdhs35 | 2021-04-15 09:14:27 |
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