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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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Transformation Protocol for BL21(DE3) Competent Cells (C2527H) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.criv4d | New England Biolabs (NEB) | This transformation protocol is to be performed directly in the C2527H tubes. (For the C2527I protocol, see here.) | New England Biolabs | https://www.neb.com/protocols/1/01/01/transformation-protocol-for-bl21-de3-competent-cells-c2527 | 1 | 2015 | New England Biolabs 2015. Transformation Protocol for BL21(DE3) Competent Cells (C2527H). protocols.io https://dx.doi.org/10.17504/protocols.io.criv4d | 2021-04-15 09:17:07 | ||
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NEBNext dA-Tailing Module (NEB #E6053) Resource Report Resource Website |
New England Biolabs, Menna Teffera | DOI:10.17504/protocols.io.4t3gwqn | New England Biolabs (NEB) | The NEBNext dA-Tailing Module has been optimized to efficiently incorporate a non-templated dAMP on the 3 ́ end of a blunt DNA fragment (1). 3 ́-dA DNA tailing prevents concadamer formation during subsequent ligation steps. DNA tailed with the NEBNext dA-Tailing module may be ligated to adaptors or cloning vectors with complementary dT overhangs. The NEBNext dA-Tailing Module is provided as a master mix to maximize efficiency and convenience in DNA sample preparation workflows. The NEBNext dA-Tailing Module has been validated by sequencing with the Il- lumina Genome Analyzer II (Illumina, Inc.) in conjunction with the NEBNext End Repair Module, NEBNext Quick Ligation Module and Phusion® High-Fidelity PCR Master Mix. For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information. | New England Biolabs, New England Biolabs | https://www.neb.com/products/e6053-nebnext-da-tailing-module#Product%20Information | 1 | 2019 | New England Biolabs, Menna Teffera 2019. NEBNext dA-Tailing Module (NEB #E6053). protocols.io https://dx.doi.org/10.17504/protocols.io.4t3gwqn | 2021-04-15 09:14:23 | ||
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Removal of Single-Stranded Extension using Mung Bean Nuclease (M0250) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.crhv35 | New England Biolabs (NEB) | This is the protocol for the removal of single-stranded extension using Mung Bean Nuclease (M0250). | New England Biolabs | https://www.neb.com/protocols/1/01/01/removal-of-single-stranded-extension | 1 | 2015 | New England Biolabs 2015. Removal of Single-Stranded Extension using Mung Bean Nuclease (M0250). protocols.io https://dx.doi.org/10.17504/protocols.io.crhv35 | 2021-04-15 09:14:21 | ||
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Purification of RNA from the Aqueous Phase Following TRIzol®/Chloroform Extraction using the Monarch® RNA Cleanup Kits Resource Report Resource Website |
New England Biolabs | New England Biolabs (NEB) | New England Biolabs | https://neb.com/protocols/2018/06/28/purification-of-rna-from-the-aqueous-phase-following-trizol-chloroform-extraction-using-the-monarch-rna-cleanup-kits | 1 | 2020 | New England Biolabs 2020. Purification of RNA from the Aqueous Phase Following TRIzol®/Chloroform Extraction using the Monarch® RNA Cleanup Kits. protocols.io https:// | 2021-04-15 09:14:22 | ||||
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Non-radioactive phosphorylation with T4 PNK Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cpdvi5 | New England Biolabs (NEB) | Protocol for non-radioactive phosphorylation with T4 PNK. | New England Biolabs | https://www.neb.com/protocols/1/01/01/non-radioactive-phosphorylation-with-t4-pnk-or-pnk3-phosphatase-minus | 1 | 2015 | New England Biolabs 2015. Non-radioactive phosphorylation with T4 PNK. protocols.io https://dx.doi.org/10.17504/protocols.io.cpdvi5 | 2021-04-15 09:14:27 | ||
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PCR with Q5® High-Fidelity 2X Master Mix (M0492) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.ciiucd | New England Biolabs (NEB) | This protocol is for PCR with Q5® High-Fidelity 2X Master Mix (M0492) | New England Biolabs | https://www.neb.com/protocols/2012/08/29/protocol-for-q5-high-fidelity-2x-master-mix-m0492 | 1 | 2014 | New England Biolabs 2014. PCR with Q5® High-Fidelity 2X Master Mix (M0492). protocols.io https://dx.doi.org/10.17504/protocols.io.ciiucd | 2021-04-15 09:14:27 | ||
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BioBrick E0546 Downstream Reaction Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cpkvkv | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/digestion-protocol-e0546 | 1 | 2015 | New England Biolabs 2015. BioBrick E0546 Downstream Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.cpkvkv | 2021-04-15 09:14:27 | |||
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Multi-temperature Double Digest (single buffer) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cjkukv | New England Biolabs (NEB) | This is the Double Digest Protocol with Standard Restriction Enzymes, in the same buffer, using two separate incubation temperatures for the enzymes. | New England Biolabs | https://www.neb.com/protocols/2014/05/07/double-digest-protocol-with-standard-restriction-enzymes | 1 | 2014 | New England Biolabs 2014. Multi-temperature Double Digest (single buffer). protocols.io https://dx.doi.org/10.17504/protocols.io.cjkukv | 2021-04-15 09:14:31 | ||
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Quick Protocol for Extraction and Purification of Genomic DNA Resource Report Resource Website |
New England Biolabs | New England Biolabs (NEB) | New England Biolabs | https://neb.com/protocols/2019/04/30/quick-protocol-for-extraction-and-purification-of-genomic-dna-using-the-monarch-genomic-dna-purification-kit-neb-t3010 | 1 | 2020 | New England Biolabs 2020. Quick Protocol for Extraction and Purification of Genomic DNA. protocols.io https:// | 2021-04-15 09:14:39 | ||||
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High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019I) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.nkvdcw6 | New England Biolabs (NEB) | This is the correct protocol if you are using the C3019I cells. If you are using the C3019H cells, please refer to this protocol. | NEB, 240 County RoadIpswich, MA 01938-2723 | https://www.neb.com/protocols/1/01/01/high-efficiency-transformation-protocol-c3019 | 5 | 2018 | New England Biolabs 2018. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019I). protocols.io https://dx.doi.org/10.17504/protocols.io.nkvdcw6 | 2021-04-15 09:14:31 | ||
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Determining Genome Targeting Efficiency using T7 Endonuclease I (M0302) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.etbbein | New England Biolabs (NEB) | T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA. This protocol describes how to determine genome targeting efficiency by digesting annealed PCR products with T7 Endonuclease I. In the first step PCR products are produced from the genomic DNA of cells whose genomes were targeted using Cas9, TALEN, ZFN etc. In the second step, the PCR products are annealed and digested with T7 Endonuclease I. Fragments are analyzed to determine the efficiency of genome targeting. | New England Biolabs | https://www.neb.com/protocols/2014/08/11/determining-genome-targeting-efficiency-using-t7-endonuclease-i | 3 | 2016 | New England Biolabs 2016. Determining Genome Targeting Efficiency using T7 Endonuclease I (M0302). protocols.io https://dx.doi.org/10.17504/protocols.io.etbbein | 2021-04-15 09:14:33 | ||
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Radioactive Labeling with T4 PNK 3' phosphatase minus Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cp9vr5 | New England Biolabs (NEB) | Radioactive Labeling with T4 Polynucleotide Kinase 3' phosphatase minus | New England Biolabs | https://www.neb.com/protocols/2012/08/24/radioactive-labeling-with-t4-pnk-or-t4-pnk-3-phosphatase-minus | 1 | 2015 | New England Biolabs 2015. Radioactive Labeling with T4 PNK 3' phosphatase minus. protocols.io https://dx.doi.org/10.17504/protocols.io.cp9vr5 | 2021-04-15 09:14:34 | ||
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Standard RNA Synthesis (E2050) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cr2v8d | New England Biolabs (NEB) | This is the synthesis protocol using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (E2050) | New England Biolabs | https://www.neb.com/protocols/2013/04/02/standard-rna-synthesis-e2050 | 1 | 2015 | New England Biolabs 2015. Standard RNA Synthesis (E2050). protocols.io https://dx.doi.org/10.17504/protocols.io.cr2v8d | 2021-04-15 09:14:54 | ||
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Gibson Assembly® Master Mix – Assembly (E2611) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cjxupm | New England Biolabs (NEB) | This is the protocol for the Gibson Assembly using the Gibson Assembly® Master Mix (E2611). | New England Biolabs | https://www.neb.com/protocols/2012/09/25/gibson-assembly-master-mix-assembly | 1 | 2015 | New England Biolabs 2015. Gibson Assembly® Master Mix – Assembly (E2611). protocols.io https://dx.doi.org/10.17504/protocols.io.cjxupm | 2021-04-15 09:14:37 | ||
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First Strand Synthesis with Reverse Transcriptase Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cjuunv | New England Biolabs (NEB) | This protocol is for First Strand Synthesis with Reverse Transcriptase | New England Biolabs | https://www.neb.com/protocols/1/01/01/first-strand-synthesis-protocol-with-reverse-transcriptase | 1 | 2015 | New England Biolabs 2015. First Strand Synthesis with Reverse Transcriptase. protocols.io https://dx.doi.org/10.17504/protocols.io.cjuunv | 2021-04-15 09:14:40 | ||
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Protocol for Exonuclease VII (NEB #M0379) Resource Report Resource Website |
New England Biolabs | New England Biolabs (NEB) | New England Biolabs | https://neb.com/protocols/2019/07/24/protocol-for-exonuclease-vii-m0379 | 1 | 2020 | New England Biolabs 2020. Protocol for Exonuclease VII (NEB #M0379). protocols.io https:// | 2021-04-15 09:14:57 | ||||
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Capped RNA E2050 Mixture Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.crzv75 | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2013/04/02/capped-rna-synthesis-e2050 | 1 | 2015 | New England Biolabs 2015. Capped RNA E2050 Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.crzv75 | 2021-04-15 09:14:39 | |||
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Cas9 M0386 Mixture Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.ch3t8m | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2014/05/01/in-vitro-digestion-of-dna-with-cas9-nuclease-s-pyogenes-m0386 | 1 | 2014 | New England Biolabs 2014. Cas9 M0386 Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.ch3t8m | 2021-04-15 09:14:41 | |||
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High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019H) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.chct2v | New England Biolabs (NEB) | This is the correct protocol if you are using the C3019H cells. If you are using the C3019I cells, please refer to this protocol. | New England Biolabs | https://www.neb.com/protocols/1/01/01/high-efficiency-transformation-protocol-c3019 | 1 | 2015 | New England Biolabs 2015. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019H). protocols.io https://dx.doi.org/10.17504/protocols.io.chct2v | 2021-04-15 09:14:44 | ||
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High Efficiency Transformation Protocol (C2987H) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.chgt3v | New England Biolabs (NEB) | This is the correct protocol if you are using the C2987H cells. If you are using the C2987I cells, please refer to this protocol. | New England Biolabs | https://www.neb.com/protocols/1/01/01/high-efficiency-transformation-protocol-c2987 | 1 | 2014 | New England Biolabs 2014. High Efficiency Transformation Protocol (C2987H). protocols.io https://dx.doi.org/10.17504/protocols.io.chgt3v | 2021-04-15 09:15:14 |
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