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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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NEBNext® Ultra™ DNA Library Prep Protocol for Illumina® With Size Selection (E7370) Resource Report Resource Website |
Isabel Gautreau | 10.17504/protocols.io.cg5ty5 | New England Biolabs (NEB) | This protocol is a group of the following 5 methods from the NEB website:1. NEBNext End Prep 2. Adaptor Ligation3. Size Selection of Adaptor-ligated DNA4. PCR Amplification5. Cleanup of PCR Amplification | New England Biolabs | https://www.neb.com/~/media/Catalog/All-Products/23EF8BC783C749D9B664D90C8C72FA73/Datacards%20or%20Manuals/manualE7370.pdf | 1 | 2015 | Isabel Gautreau 2015. NEBNext® Ultra™ DNA Library Prep Protocol for Illumina® With Size Selection (E7370). protocols.io dx.doi.org/10.17504/protocols.io.cg5ty5 | 2021-03-29 03:12:04 | ||
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Immunoprecipitation using Protein A/G Magnetic Beads Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.crav2d | New England Biolabs (NEB) | This is the protocol for immunoprecipitation using Protein A/G Magnetic Beads. | New England Biolabs | https://www.neb.com/protocols/1/01/01/immunoprecipitation-using-protein-ag-magnetic-beads | 1 | 2015 | New England Biolabs 2015. Immunoprecipitation using Protein A/G Magnetic Beads. protocols.io dx.doi.org/10.17504/protocols.io.crav2d | 2021-03-29 03:08:14 | ||
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Ligation Protocol WITH T4 DNA Ligase (M0202) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cdks4v | New England Biolabs (NEB) | Please see the NEB website for more information. | New England Biolabs | https://www.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202 | 1 | 2015 | New England Biolabs 2015. Ligation Protocol WITH T4 DNA Ligase (M0202). protocols.io dx.doi.org/10.17504/protocols.io.cdks4v | 2021-03-29 03:08:14 | ||
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NEBNext® Ultra™ DNA Library Prep Protocol for Illumina® With Size Selection (E7370) Resource Report Resource Website |
Isabel Gautreau | 10.17504/protocols.io.gx8bxrw | New England Biolabs (NEB) | This protocol is a group of the following 5 methods from the NEB website:1. NEBNext End Prep 2. Adaptor Ligation3. Size Selection of Adaptor-ligated DNA4. PCR Amplification5. Cleanup of PCR Amplification | New England Biolabs | https://www.neb.com/~/media/Catalog/All-Products/23EF8BC783C749D9B664D90C8C72FA73/Datacards%20or%20Manuals/manualE7370.pdf | 2 | 2017 | Isabel Gautreau 2017. NEBNext® Ultra™ DNA Library Prep Protocol for Illumina® With Size Selection (E7370). protocols.io dx.doi.org/10.17504/protocols.io.gx8bxrw | 2021-03-29 03:08:15 | ||
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Radioactive Labeling with T4 PNK (M0201S) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cp7vrm | New England Biolabs (NEB) | Radioactive Labeling with T4 Polynucleotide Kinase | New England Biolabs | https://www.neb.com/protocols/2012/08/24/radioactive-labeling-with-t4-pnk-or-t4-pnk-3-phosphatase-minus | 1 | 2015 | New England Biolabs 2015. Radioactive Labeling with T4 PNK (M0201S). protocols.io dx.doi.org/10.17504/protocols.io.cp7vrm | 2021-03-29 03:08:19 | ||
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Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.p26dqhe | New England Biolabs (NEB) | Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010).Quickly and easily purify up to 100 µg of high-quality total RNA from multiple sample types – all with one kit!For use with blood, cells and tissuesAlso works with tough to lyse samples (bacteria, yeast, plant)Effectively purifies total RNA of all sizes, including small RNAs >20 ntEfficient genomic DNA removal (column and DNase I-based)Contains Proteinase K for processing of tissues and blood samplesIncludes RNA Protection Reagent for sample preservationExcellent valueKit components available separately | New England Biolabs | https://www.neb.com/protocols/2017/11/28/quick-protocol-for-monarch-total-rna-miniprep-kit-neb-t2010 | 2 | 2018 | New England Biolabs 2018. Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010). protocols.io dx.doi.org/10.17504/protocols.io.p26dqhe | 2021-03-29 03:08:18 | ||
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In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.ch2t8d | New England Biolabs (NEB) | Cas9 Nuclease, S. pyogenes (Cas9), is a double-stranded DNA endonuclease that is guided to its target by sequence complementarity of a small RNA loaded into the protein. This protocol describes how to digest double-stranded DNA in vitro using Cas9 and a single guide RNA (sgRNA). | New England Biolabs | https://www.neb.com/protocols/2014/05/01/in-vitro-digestion-of-dna-with-cas9-nuclease-s-pyogenes-m0386 | 1 | 2014 | New England Biolabs 2014. In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386). protocols.io dx.doi.org/10.17504/protocols.io.ch2t8d | 2021-03-29 03:08:22 | ||
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NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB #E7546) Resource Report Resource Website |
Menna Teffera, New England Biolabs | 10.17504/protocols.io.4nngvde | New England Biolabs (NEB) | The NEBNext Ultra II End Repair/dA-Tailing Module is optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5 ́ phosphorylated, 3 ́ dA-tailed ends. This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext™ Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction. This module is also compatible with some Oxford Nanopore MinION™workflows.This module is designed for use with NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600 or #E7535), NEBNext Ultra II Ligation Module (NEB #E7595), and NEBNext Ultra II Q5 Master Mix (NEB #M0544). Kits that include reagents for every step in the Ultra II DNA library construction workflow are also available (NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) and NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB #E7103). | New England Biolabs, New England Biolabs | https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7546.pdf?rev=221a72d9f6624cfda4fc2fe760691f02 | 1 | 2019 | Menna Teffera, New England Biolabs 2019. NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB #E7546). protocols.io dx.doi.org/10.17504/protocols.io.4nngvde | 2021-03-29 03:08:22 | ||
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Dephosphorylation using rSAP in Restriction Enzyme Reaction(M0371) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.nkxdcxn | New England Biolabs (NEB) | This protocol is for dephosphorylation of 5´-ends of DNA using rSAP in restriction enzyme reaction (M0371) | NEB, Ipswich MA | https://www.neb.com/protocols/2013/06/10/protocol-for-dephosphorylation-of-5-ends-of-dna-in-restriction-enzyme-reaction-m0371 | 3 | 2018 | New England Biolabs 2018. Dephosphorylation using rSAP in Restriction Enzyme Reaction(M0371). protocols.io dx.doi.org/10.17504/protocols.io.nkxdcxn | 2021-03-29 03:08:23 | ||
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In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386) Resource Report Resource Website |
Breton Hornblower | 10.17504/protocols.io.hwkb7cw | New England Biolabs (NEB) | Cas9 Nuclease, S. pyogenes (Cas9), is a double-stranded DNA endonuclease that is guided to its target by sequence complementarity of a small RNA loaded into the protein. This protocol describes how to digest double-stranded DNA in vitro using Cas9 and a single guide RNA (sgRNA). | https://www.neb.com/protocols/2014/05/01/in-vitro-digestion-of-dna-with-cas9-nuclease-s-pyogenes-m0386 | 2 | 2017 | Breton Hornblower 2017. In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386). protocols.io dx.doi.org/10.17504/protocols.io.hwkb7cw | 2021-03-29 03:08:28 | |||
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PNGase F Protocol, Non-Denaturing Conditions Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cqivud | New England Biolabs (NEB) | This is a generic PNGase F protocol with non-denaturing reaction conditions. It is appropriate for both P0704 and P0708. Typical reaction conditions are below. | New England Biolabs | https://www.neb.com/protocols/2014/07/31/pngase-f-protocol | 1 | 2015 | New England Biolabs 2015. PNGase F Protocol, Non-Denaturing Conditions. protocols.io dx.doi.org/10.17504/protocols.io.cqivud | 2021-03-29 03:09:31 | ||
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Blunting Protocol (M0203) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.iswcefe | New England Biolabs (NEB) | Protocol for blunting ends by 3' overhang removal and fill-in of 3' recessed (5' overhang) ends using T4 DNA Polymerase. | NEB, Ipswich, MA | https://www.neb.com/protocols/2014/01/13/protocol-for-blunting-ends-by-3-overhang-removal-and-fill-in-of-3-recessed-5-overhang-ends-using1 | 2 | 2018 | New England Biolabs 2018. Blunting Protocol (M0203). protocols.io dx.doi.org/10.17504/protocols.io.iswcefe | 2021-03-29 03:09:45 | ||
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FirstStrand with RT Reaction Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cjvun5 | New England Biolabs (NEB) | New England Biolabs | 1 | 2014 | New England Biolabs 2014. FirstStrand with RT Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cjvun5 | 2021-03-29 03:09:51 | ||||
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Standard RNA Synthesis Mixture for E2050 Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cr3v8m | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2013/04/02/standard-rna-synthesis-e2050 | 1 | 2015 | New England Biolabs 2015. Standard RNA Synthesis Mixture for E2050. protocols.io dx.doi.org/10.17504/protocols.io.cr3v8m | 2021-03-29 03:08:30 | |||
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T4 DNA Ligase Reaction Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cf7trm | New England Biolabs (NEB) | This is the reaction for the "Ligation Protocol with T4 DNA Ligase" | New England Biolabs | https://www.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202 | 1 | 2015 | New England Biolabs 2015. T4 DNA Ligase Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cf7trm | 2021-03-29 03:08:31 | ||
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Quick Protocol for Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.n3vdgn6 | New England Biolabs (NEB) | This is the "quick" version of Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030). For the full protocol, please click here. | New England Biolabs | https://www.neb.com/protocols/2015/12/08/quick-protocol-for-monarch-pcr-dna-cleanup-kit-5-g-t1030 | 3 | 2018 | New England Biolabs 2018. Quick Protocol for Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030). protocols.io dx.doi.org/10.17504/protocols.io.n3vdgn6 | 2021-03-29 03:08:36 | ||
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Ligation Protocol with T4 DNA Ligase (M0202) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.irscd6e | New England Biolabs (NEB) | Please see the NEB website for more information. | New England Biolabs | https://www.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202 | 2 | 2017 | New England Biolabs 2017. Ligation Protocol with T4 DNA Ligase (M0202). protocols.io dx.doi.org/10.17504/protocols.io.irscd6e | 2021-03-29 03:08:38 | ||
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Dephosphorylation using rSAP in Restriction Enzyme Reaction(M0371) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cgvtw5 | New England Biolabs (NEB) | This protocol is for dephosphorylation of 5´-ends of DNA using rSAP in restriction enzyme reaction (M0371) | New England Biolabs | https://www.neb.com/protocols/2013/06/10/protocol-for-dephosphorylation-of-5-ends-of-dna-in-restriction-enzyme-reaction-m0371 | 1 | 2015 | New England Biolabs 2015. Dephosphorylation using rSAP in Restriction Enzyme Reaction(M0371). protocols.io dx.doi.org/10.17504/protocols.io.cgvtw5 | 2021-03-29 03:07:57 | ||
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Gibson Assembly® Protocol (E5510) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cdms45 | New England Biolabs (NEB) | This is the protocol for the Gibson Assembly using the Gibson Assembly® Cloning Kit (E5510). | New England Biolabs | https://www.neb.com/protocols/2012/12/11/gibson-assembly-protocol-e5510 | 1 | 2015 | New England Biolabs 2015. Gibson Assembly® Protocol (E5510). protocols.io dx.doi.org/10.17504/protocols.io.cdms45 | 2021-03-29 03:07:58 | ||
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Reaction Mixture for M0371 Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cgutwv | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2013/06/10/protocol-for-dephosphorylation-of-5-ends-of-dna-in-restriction-enzyme-reaction-m0371 | 1 | 2015 | New England Biolabs 2015. Reaction Mixture for M0371. protocols.io dx.doi.org/10.17504/protocols.io.cgutwv | 2021-03-29 03:08:00 |
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