Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Immunohistochemistry Protocol for Frozen Sections Resource Report Resource Website |
Kelsey Knight | DOI:10.17504/protocols.io.8wahxae | BioLegend | The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary antibody, biotinylated secondary antibody and streptavidin-horseradish peroxidase (SAv-HRP) and DAB detection system. Because each antigen differs in terms of requirement for fixation, amplification step, etc., it is not possible to write an inclusive protocol that will work for all antigens. The user must determine optimal conditions for each antigen of interest. Many protocols for staining individual antigens, as well as useful tips and troubleshooting guides for immunohistochemistry, can be found at the IHC World web site (https://www.ihcworld.com/). | BioLegend | https://www.biolegend.com/protocols/immunohistochemistry-protocol-for-frozen-sections/4288/ | 3 | 2019 | Kelsey Knight 2019. Immunohistochemistry Protocol for Frozen Sections. protocols.io https://dx.doi.org/10.17504/protocols.io.8wahxae | 2021-04-15 09:16:23 | ||
|
MojoSort™ Nanobeads Column Protocol - 1 Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.69dhh26 | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-nanobeads-column-protocol-1/4593/ | 1 | 2019 | Sam Li 2019. MojoSort™ Nanobeads Column Protocol - 1. protocols.io https://dx.doi.org/10.17504/protocols.io.69dhh26 | 2021-04-15 09:16:27 | ||
|
Flex-T™ HLA Class I ELISA Protocol Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.mcsc2we | BioLegend | The HLA class I ELISA is an enzyme immunoassay based on the detection of β2-microglobulin subunit of HLA class I complexes, after capturing the complex through the conjugated biotin. To this end, biotinylated HLA class I complex is first captured in streptavidin coated microtiter wells. Subsequently, HRP-conjugated anti-human β2-microglobulin is added to detect intact HLA class I complexes. Only intact HLA class I complexes are recognized. Peptides with high affinity binding will be clearly detected by this ELISA technique, while peptides with a moderate to low binding affinity for HLA class I provide a moderate to non-detectable signal. This protocol is designed to evaluate the efficiency of peptide exchange when using the Flex-T™ system. | BioLegend | 3 | 2017 | Kelsey Miller 2017. Flex-T™ HLA Class I ELISA Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.mcsc2we | 2021-04-15 09:16:26 | |||
|
MojoSort™ Mouse CD11c Nanobeads Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7xjhpkn | BioLegend | Product description and procedure summary: The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. Sample Preparation: Enzymatic digestion of mouse spleen is recommended to achieve the highest purity and yield of CD11c+ cells. There are several protocols published that can be applied. As a general guideline, cut mouse spleen into pieces and incubate in 0.5 mg/ml Collagenase for 30 to 60 minutes at room temperature or 37°C. Place the tube in a rocking platform with continuous agitation or gently pipette every 10 minutes. Alternatively, inject 1 ml of enzymes solution in the uncut organ. Force the tissue through a 70µm filter to prepare a single cell suspension, and wash with complete media. Resuspend cells in 0.1 mg/ml DNase 1 solution and incubate at room temperature for 10 minutes. Again, filter cells through a 70 µm filter and wash with complete media. Resuspend in complete media or MojoSort™ Buffer and keep on ice until ready to use. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-cd11c-nanobeads-protocol/4401/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse CD11c Nanobeads Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7xjhpkn | 2021-04-15 09:16:27 | ||
|
Immunohistochemistry Protocol for Paraffin-Embedded Sections Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.97bh9in | BioLegend | BioLegend | https://www.biolegend.com/protocols/immunohistochemistry-protocol-for-paraffin-embedded-sections/4256/ | 3 | 2019 | Sam Li 2019. Immunohistochemistry Protocol for Paraffin-Embedded Sections. protocols.io https://dx.doi.org/10.17504/protocols.io.97bh9in | 2021-04-15 09:16:31 | |||
|
Chromatin Immunoprecipitation (ChIP) Assay Protocol Resource Report Resource Website |
Kelsey Knight | DOI:10.17504/protocols.io.tj7ekrn | BioLegend | Reagents - Basic cell culture media: respective to the cell line used and experimental design Distilled water: Invitrogen® UltraPure™ Distilled Water (catalog number 19977015) or equivalent product from other vendor.Highthroughput (HT) Pro A or G 96 well plate or Spin Column (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)96-well collection plate: USA scientific® TempPlate non-skirted 96-well PCR plate natural (catalog number 1402-9596) or equivalent product from other vendor.QIAquick™ PCR purification kit (Qiagen®; catalog number 28104) or equivalent product from other vendor.Hypotonic Buffer (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Digestion Buffer (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Lysis Buffer (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Column Conditioning Buffer (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Wash Buffer 1 (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Wash Buffer 2 (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Wash Buffer 3 (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Elution Buffer (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)5M NaCl: dissolve 292g of NaCl in 800mL of H2O. Adjust the volume to 1L with H2O.1M NaHCO3: dissolve 12.6g of NaHCO3 in 100 mL of H2O. Adjust the volume to 150 mL with H2O.Protease Inhibitor Cocktail (PIC): Cell signaling Technology® (catalog number 5871) or equivalent product from other vendor.Proteinase K: Qiagen® (catalog number 19133) or equivalent product from other vendor.Proteinase K stop solution: 100mM Phenylmethylsulfonyl fluoride (PMSF). Weigh 1.742g PMSF (Sigma-Aldrich®, catalog number 10837091001) and add DMSO (Sigma-Aldrich®, catalog number D2650) to a final volume of 100mL. Dissolve completely.PBS: HyClone™ Phosphate Buffered Saline (1X) (catalog number SH30256.01) or equivalent product from other vendor.Formaldehyde: VWR® 37% w/w aqueous solution (catalog number 97064-604) or equivalent product from other vendor.0.65M Glycine: Dissolve 488g of glycine into 1L of H2O to make 6.5M glycine (10X). Dilute with PBS to make 1X before each experiment. Micrococcal Nuclease: Cell Signaling Technologies® (catalog number 10011S) or equivalent product from other vendor.1.3-1.5% Agarose gel: dissolve 1.3-1.5g of agarose powder into 100mL of TAE buffer by microwave heating. Visualize DNA under UV light by adding appropriate amount of Ethidium Bromide, or use an equivalent method.Add appropriate amount of Ethidium Bromide to cooled down gel solution to visualize DNA under UV light.0.5M EDTA: Dissolve 186.1g EDTA into 700mL of H2O. Adjust the volume to 1L with H2O.100bp ladder: NEB® (catalog number N0467S) or equivalent product from other vendor.Materials -Heat blockRotatorCentrifugeMicrocentrifuge37ºC incubatorNanodropSonicatorUV light imagerRocking platform | BioLegend | http://www.biolegend.com/media_assets/support_protocol/ChIP_Assay_Technical_Protocol_v8.pdf | 3 | 2018 | Kelsey Knight 2018. Chromatin Immunoprecipitation (ChIP) Assay Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.tj7ekrn | 2021-04-15 09:16:56 | ||
|
Genotypic and phenotypic ?-Lactam resistance and presence of PVL gene in Staphylococci from dry bovine udder Resource Report Resource Website |
Vinodkumar Kulangara, Neetha Nair, Asok Sivasailam, Suchithra Sasidharan, Justin Davis and Radhika Syam | DOI:10.17504/protocols.io.jhccj2w | BioLegend | Dairy cows affected with subclinical mastitis can be sources of virulent, antimicrobial-resistant Staphylococci to humans by the excretion of the bacteria through their milk. This study focussed on the phenotypic and genotypic antibiotic resistance patterns of Staphylococci isolated from dairy cows during the early dry period. Among 96 isolates of Gram positive cocci from 157 cows, 76 were identified as Coagulase Negative Staphylococci and the remaining twenty were Staphylococcus aureus. Typical amplicons of coagulase gene were obtained for all twenty samples of S. aureus with three major coagulase types being identified as giving 627 bp (40 %), 910 bp (35 %) and 710 bp (25 %) long PCR products. The groEL gene was amplified in PCR of all 76 isolates of Coagulase Negative Staphylococci, and incubation of PCR products with restriction enzyme PvuII yielded three distinct PCR-RFLP fragment patterns bearing resemblance to S. chromogenes and S. hyicus. Highest sensitivity of Coagulase Negative Staphylococci was noted for Azithromycin (92.5%) and the least to Tetracyclines (76.3%), whereas for S. aureus, it was Cefoperazone (95%) and Azithromycin (72.2%) respectively. Phenotypic resistance to Oxacillin (25 isolates), and Cefoxitin (11 isolates) was detected by dilution method with a commercial strip (Ezy MICTM). Genotypic resistance to b-Lactam antibiotics was found in 65 (34 with mecA gene and 31 with blaZ gene) isolates. Eighteen isolates possessed both the genes, with the PVL gene for virulence being detected in five of them. Nine isolates which had mecA gene were phenotypically susceptible to oxacillin while phenotypic resistance to oxacillin was observed in seven isolates that did not have either mecA or blaZ gene. This is the first report of persistent Staphylococcal infections possessing PVL gene and high level of genotypic resistance to b-Lactam antibiotics in small- holder dairy cattle from India. | Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India, , , , | 1 | 2017 | Vinodkumar Kulangara, Neetha Nair, Asok Sivasailam, Suchithra Sasidharan, Justin Davis and Radhika Syam 2017. Genotypic and phenotypic ?-Lactam resistance and presence of PVL gene in Staphylococci from dry bovine udder . protocols.io https://dx.doi.org/10.17504/protocols.io.jhccj2w | 2021-04-15 09:16:38 | |||
|
MojoSort™ Human CD4 T Cell Selection Kit Column Protocol - CD4 Nanobeads Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7tyhnpw | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-cd4-t-cell-selection-kit-column-protocol-cd4-nanobeads/4762/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human CD4 T Cell Selection Kit Column Protocol - CD4 Nanobeads. protocols.io https://dx.doi.org/10.17504/protocols.io.7tyhnpw | 2021-04-15 09:17:02 | ||
|
True-Nuclear™ Transcription Factor Staining Protocol for 96-Well, U-Bottom Plate Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bacriav6 | BioLegend | BioLegend | https://www.biolegend.com/protocols/true-nuclear-transcription-factor-staining-protocol-for-96-well-u-bottom-plate/4246/ | 5 | 2019 | Sam Li 2019. True-Nuclear™ Transcription Factor Staining Protocol for 96-Well, U-Bottom Plate. protocols.io https://dx.doi.org/10.17504/protocols.io.bacriav6 | 2021-04-15 09:17:00 | |||
|
Th17 Polarization of Mouse CD4+ Cells Resource Report Resource Website |
Kelsey Knight | DOI:10.17504/protocols.io.tnhemb6 | BioLegend | BioLegend | https://www.biolegend.com/protocols/th17-polarization-of-mouse-cd4--cells/4284/ | 2 | 2018 | Kelsey Knight 2018. Th17 Polarization of Mouse CD4+ Cells. protocols.io https://dx.doi.org/10.17504/protocols.io.tnhemb6 | 2021-04-15 09:16:51 | |||
|
Intracellular Staining With True-Phos™ Perm Buffer in Whole Blood Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bac6iaze | BioLegend | BioLegend | https://www.biolegend.com/protocols/intracellular-staining-with-true-phos-perm-buffer-in-whole-blood/4261/ | 2 | 2019 | Sam Li 2019. Intracellular Staining With True-Phos™ Perm Buffer in Whole Blood. protocols.io https://dx.doi.org/10.17504/protocols.io.bac6iaze | 2021-04-15 09:16:56 | |||
|
Immunohistochemistry Protocol for Paraffin-Embedded Sections Resource Report Resource Website |
Kelsey Knight | DOI:10.17504/protocols.io.tkjekun | BioLegend | BioLegend | https://www.biolegend.com/protocols/immunohistochemistry-protocol-for-paraffin-embedded-sections/4256/ | 2 | 2018 | Kelsey Knight 2018. Immunohistochemistry Protocol for Paraffin-Embedded Sections. protocols.io https://dx.doi.org/10.17504/protocols.io.tkjekun | 2021-04-15 09:17:03 | |||
|
Anti-BrdU Staining Protocols Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e23bggn | BioLegend | Protocols for Anti-BrdU Staining Using DNAse with Surface and Fluorescent Proteinsand Anti-BrdU Staining Using 70% Ethanol and 2N HCl | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BrdU_Staining_Protocols_05102016.pdf | 1 | 2016 | Kelsey Miller 2016. Anti-BrdU Staining Protocols. protocols.io https://dx.doi.org/10.17504/protocols.io.e23bggn | 2021-04-15 09:17:04 | ||
|
Immunohistochemistry Protocol for Ultra Streptavidin Detection Kits (USA) Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e2kbgcw | BioLegend | BioLegend | http://www.biolegend.com/media_assets/support_protocol/IHC_Protocol_for_Ultra_Streptavidin_Detection_Kits_USA_090815.pdf | 1 | 2016 | Kelsey Miller 2016. Immunohistochemistry Protocol for Ultra Streptavidin Detection Kits (USA). protocols.io https://dx.doi.org/10.17504/protocols.io.e2kbgcw | 2021-04-15 09:17:03 | |||
|
True-Nuclear™ Transcription Factor Staining Protocol for 96-Well, U-Bottom Plate Resource Report Resource Website |
BioLegend, Inc. | DOI:10.17504/protocols.io.exsbfne | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/True-Nuclear_Trademark_Transcription_Factor_Staining_Protocol.pdf | 1 | 2016 | BioLegend, Inc. 2016. True-Nuclear™ Transcription Factor Staining Protocol for 96-Well, U-Bottom Plate. protocols.io https://dx.doi.org/10.17504/protocols.io.exsbfne | 2021-04-15 09:17:04 | |||
|
MojoSort™ Mouse CX3CR1 Selection Kit Protocol Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.fyqbpvw | BioLegend | This procedure is optimized for the isolation of 106 cells per test. If working with fewer than 106 cells, keep volumes as indicated for 106 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Product description and procedure summary: Target cells are positively selected or depleted by incubating the sample with the biotin anti‐mouse CX3CR1 antibody followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. These are the CX3CR1+ cells, do not discard them if those are the cells of interest. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Mouse_CX3CR1_Selection_Kit_Protocol_10032016.pdfhttp://www.biolegend.com/media_assets/support_protocol/MojoSort_Mouse_CX3CR1_Selection_Kit_Protocol_10032016.pdf | 1 | 2016 | Kelsey Miller 2016. MojoSort™ Mouse CX3CR1 Selection Kit Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.fyqbpvw | 2021-04-15 09:17:04 | ||
|
Immunofluorescent Staining of Whole Blood Resource Report Resource Website |
BioLegend, Inc. | DOI:10.17504/protocols.io.hwdb7a6 | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/BioLegend_Surface_Staining_Flow_Protocol_060215.pdf | 2 | 2017 | BioLegend, Inc. 2017. Immunofluorescent Staining of Whole Blood. protocols.io https://dx.doi.org/10.17504/protocols.io.hwdb7a6 | 2021-04-15 09:17:08 | |||
|
Preparation of Brain Samples for LEGEND MAX™ Beta Amyloid ELISA Resource Report Resource Website |
Kelsey Knight | DOI:10.17504/protocols.io.tm3ek8n | BioLegend | BioLegend | 1 | 2018 | Kelsey Knight 2018. Preparation of Brain Samples for LEGEND MAX™ Beta Amyloid ELISA. protocols.io https://dx.doi.org/10.17504/protocols.io.tm3ek8n | 2021-04-15 09:14:21 | ||||
|
Immunohistochemistry Protocol for Beta Amyloid Products using USA Detection Kit Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.8x4hxqw | BioLegend | BioLegend | https://www.biolegend.com/protocols/immunohistochemistry-protocol-for-beta-amyloid-products-using-usa-detection-kit/4259/ | 2 | 2019 | Sam Li 2019. Immunohistochemistry Protocol for Beta Amyloid Products using USA Detection Kit. protocols.io https://dx.doi.org/10.17504/protocols.io.8x4hxqw | 2021-04-15 09:14:21 | |||
|
MojoSort™ Mouse NK Cell Isolation Kit Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7yshpwe | BioLegend | Product description and procedure summary: Target cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads (Cat. No. 480015/480016). The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes.Sample Preparation: Dead cell removal prior to cell isolation is recommended to achieve the highest purity and yield of mouse NK cells. Dead cells can be removed using appropriate centrifugation media or by other methods. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-nk-cell-isolation-kit-protocol/4757/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse NK Cell Isolation Kit Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7yshpwe | 2021-04-15 09:14:26 |
Can't find your Protocol?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific protocol and you know the DOI of the protocol already, it's easier to enter a DOI to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your protocol in the search results, please help us by adding it into the system — it's easy. Create and publish your protocols at Protocols.io.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
