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Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the synthesis protocol using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (E2050)
Proper citation: New England Biolabs 2015. Standard RNA Synthesis (E2050). protocols.io https://dx.doi.org/10.17504/protocols.io.cr2v8d Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for the Gibson Assembly using the Gibson Assembly® Master Mix (E2611).
Proper citation: New England Biolabs 2015. Gibson Assembly® Master Mix – Assembly (E2611). protocols.io https://dx.doi.org/10.17504/protocols.io.cjxupm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocol is for First Strand Synthesis with Reverse Transcriptase
Proper citation: New England Biolabs 2015. First Strand Synthesis with Reverse Transcriptase. protocols.io https://dx.doi.org/10.17504/protocols.io.cjuunv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Capped RNA E2050 Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.crzv75 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2014. Cas9 M0386 Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.ch3t8m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the correct protocol if you are using the C3019H cells. If you are using the C3019I cells, please refer to this protocol.
Proper citation: New England Biolabs 2015. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019H). protocols.io https://dx.doi.org/10.17504/protocols.io.chct2v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the correct protocol if you are using the C2987H cells. If you are using the C2987I cells, please refer to this protocol.
Proper citation: New England Biolabs 2014. High Efficiency Transformation Protocol (C2987H). protocols.io https://dx.doi.org/10.17504/protocols.io.chgt3v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Loading Marker Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.cq5vy5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of RNA into high quality directional (strand-specific) libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow has minimal hands-on time and is compatible with poly(A) mRNA enrichment and rRNA depletion methods.
Proper citation: New England Biolabs 2018. Protocol for use with Purified mRNA or rRNA Depleted RNA and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765). protocols.io https://dx.doi.org/10.17504/protocols.io.t8xerxn Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Q5U Hot Start High-Fidelity DNA Polymerase is a modified version of Q5®High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.
Proper citation: New England Biolabs 2020. PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515): General PCR, USER®Cloning, dUTP incorporation/Carryover prevention. protocols.io https://dx.doi.org/10.17504/protocols.io.7schnaw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Radioactive T4PNK Minus Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.cqavsd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010).Quickly and easily purify up to 100 µg of high-quality total RNA from multiple sample types – all with one kit!For use with blood, cells and tissuesAlso works with tough to lyse samples (bacteria, yeast, plant)Effectively purifies total RNA of all sizes, including small RNAs >20 ntEfficient genomic DNA removal (column and DNase I-based)Contains Proteinase K for processing of tissues and blood samplesIncludes RNA Protection Reagent for sample preservationExcellent valueKit components available separately
Proper citation: New England Biolabs 2020. Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010). protocols.io https://dx.doi.org/10.17504/protocols.io.bp9cmr2w Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2014. Mixture for M0290 CIP. protocols.io https://dx.doi.org/10.17504/protocols.io.chrt55 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010).Quickly and easily purify up to 100 µg of high-quality total RNA from multiple sample types – all with one kit!For use with blood, cells and tissuesAlso works with tough to lyse samples (bacteria, yeast, plant)Effectively purifies total RNA of all sizes, including small RNAs >20 ntEfficient genomic DNA removal (column and DNase I-based)Contains Proteinase K for processing of tissues and blood samplesIncludes RNA Protection Reagent for sample preservationExcellent valueKit components available separately
Proper citation: New England Biolabs 2018. Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010). protocols.io https://dx.doi.org/10.17504/protocols.io.nyadfse Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the Double Digest Protocol with Standard Restriction Enzymes, using a common reaction and same incubation temperature for both enzymes.
Proper citation: New England Biolabs 2014. Single-temperature Double Digest. protocols.io https://dx.doi.org/10.17504/protocols.io.cjhuj5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB), Coronavirus Method Development Community
Summary: This protocol utilizes the Monarch Total RNA Miniprep Kit to purify RNA from buccal swabs, nasopharyngeal samples, and saliva.
Proper citation: New England Biolabs 2020. RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit. protocols.io https://dx.doi.org/10.17504/protocols.io.be93jh8n Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Mixture for M0273 Taq PCR. protocols.io https://dx.doi.org/10.17504/protocols.io.cicuav Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2014. 10X RT Buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.cjwupd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. PNGase F Mixture 2. protocols.io https://dx.doi.org/10.17504/protocols.io.cqhvt5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for the Gibson Assembly using the Gibson Assembly® Cloning Kit (E5510).
Proper citation: New England Biolabs 2015. Gibson Assembly® Protocol (E5510). protocols.io https://dx.doi.org/10.17504/protocols.io.cdms45 Copy
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