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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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Standard RNA Synthesis (E2050) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cr2v8d | New England Biolabs (NEB) | This is the synthesis protocol using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (E2050) | New England Biolabs | https://www.neb.com/protocols/2013/04/02/standard-rna-synthesis-e2050 | 1 | 2015 | New England Biolabs 2015. Standard RNA Synthesis (E2050). protocols.io https://dx.doi.org/10.17504/protocols.io.cr2v8d | 2021-04-15 09:14:54 | ||
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Gibson Assembly® Master Mix – Assembly (E2611) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cjxupm | New England Biolabs (NEB) | This is the protocol for the Gibson Assembly using the Gibson Assembly® Master Mix (E2611). | New England Biolabs | https://www.neb.com/protocols/2012/09/25/gibson-assembly-master-mix-assembly | 1 | 2015 | New England Biolabs 2015. Gibson Assembly® Master Mix – Assembly (E2611). protocols.io https://dx.doi.org/10.17504/protocols.io.cjxupm | 2021-04-15 09:14:37 | ||
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First Strand Synthesis with Reverse Transcriptase Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cjuunv | New England Biolabs (NEB) | This protocol is for First Strand Synthesis with Reverse Transcriptase | New England Biolabs | https://www.neb.com/protocols/1/01/01/first-strand-synthesis-protocol-with-reverse-transcriptase | 1 | 2015 | New England Biolabs 2015. First Strand Synthesis with Reverse Transcriptase. protocols.io https://dx.doi.org/10.17504/protocols.io.cjuunv | 2021-04-15 09:14:40 | ||
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Capped RNA E2050 Mixture Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.crzv75 | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2013/04/02/capped-rna-synthesis-e2050 | 1 | 2015 | New England Biolabs 2015. Capped RNA E2050 Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.crzv75 | 2021-04-15 09:14:39 | |||
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Cas9 M0386 Mixture Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.ch3t8m | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2014/05/01/in-vitro-digestion-of-dna-with-cas9-nuclease-s-pyogenes-m0386 | 1 | 2014 | New England Biolabs 2014. Cas9 M0386 Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.ch3t8m | 2021-04-15 09:14:41 | |||
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High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019H) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.chct2v | New England Biolabs (NEB) | This is the correct protocol if you are using the C3019H cells. If you are using the C3019I cells, please refer to this protocol. | New England Biolabs | https://www.neb.com/protocols/1/01/01/high-efficiency-transformation-protocol-c3019 | 1 | 2015 | New England Biolabs 2015. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019H). protocols.io https://dx.doi.org/10.17504/protocols.io.chct2v | 2021-04-15 09:14:44 | ||
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High Efficiency Transformation Protocol (C2987H) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.chgt3v | New England Biolabs (NEB) | This is the correct protocol if you are using the C2987H cells. If you are using the C2987I cells, please refer to this protocol. | New England Biolabs | https://www.neb.com/protocols/1/01/01/high-efficiency-transformation-protocol-c2987 | 1 | 2014 | New England Biolabs 2014. High Efficiency Transformation Protocol (C2987H). protocols.io https://dx.doi.org/10.17504/protocols.io.chgt3v | 2021-04-15 09:15:14 | ||
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Loading Marker Mixture Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cq5vy5 | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/suggested-protocol-for-loading-a-dna-ladder-marker | 1 | 2015 | New England Biolabs 2015. Loading Marker Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.cq5vy5 | 2021-04-15 09:15:25 | |||
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Protocol for use with Purified mRNA or rRNA Depleted RNA and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.t8xerxn | New England Biolabs (NEB) | The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of RNA into high quality directional (strand-specific) libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow has minimal hands-on time and is compatible with poly(A) mRNA enrichment and rRNA depletion methods. | New England Biolabs | 1 | 2018 | New England Biolabs 2018. Protocol for use with Purified mRNA or rRNA Depleted RNA and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765). protocols.io https://dx.doi.org/10.17504/protocols.io.t8xerxn | 2021-04-15 09:14:52 | |||
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PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515): General PCR, USER®Cloning, dUTP incorporation/Carryover prevention Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.7schnaw | New England Biolabs (NEB) | Q5U Hot Start High-Fidelity DNA Polymerase is a modified version of Q5®High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases. | New England Biolabs | https://www.neb.com/protocols/2019/07/02/pcr-using-q5u-hot-start-high-fidelity-dna-polymerase-neb-m0515 | 1 | 2020 | New England Biolabs 2020. PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515): General PCR, USER®Cloning, dUTP incorporation/Carryover prevention. protocols.io https://dx.doi.org/10.17504/protocols.io.7schnaw | 2021-04-15 09:14:54 | ||
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Radioactive T4PNK Minus Reaction Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cqavsd | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2012/08/24/radioactive-labeling-with-t4-pnk-or-t4-pnk-3-phosphatase-minus | 1 | 2015 | New England Biolabs 2015. Radioactive T4PNK Minus Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.cqavsd | 2021-04-15 09:15:28 | |||
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Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.bp9cmr2w | New England Biolabs (NEB) | Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010).Quickly and easily purify up to 100 µg of high-quality total RNA from multiple sample types – all with one kit!For use with blood, cells and tissuesAlso works with tough to lyse samples (bacteria, yeast, plant)Effectively purifies total RNA of all sizes, including small RNAs >20 ntEfficient genomic DNA removal (column and DNase I-based)Contains Proteinase K for processing of tissues and blood samplesIncludes RNA Protection Reagent for sample preservationExcellent valueKit components available separately | New England Biolabs | https://www.neb.com/protocols/2017/11/28/quick-protocol-for-monarch-total-rna-miniprep-kit-neb-t2010 | 3 | 2020 | New England Biolabs 2020. Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010). protocols.io https://dx.doi.org/10.17504/protocols.io.bp9cmr2w | 2021-04-15 09:15:27 | ||
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Mixture for M0290 CIP Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.chrt55 | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/protocol-for-dephosphorylating-with-cip | 1 | 2014 | New England Biolabs 2014. Mixture for M0290 CIP. protocols.io https://dx.doi.org/10.17504/protocols.io.chrt55 | 2021-04-15 09:15:29 | |||
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Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.nyadfse | New England Biolabs (NEB) | Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010).Quickly and easily purify up to 100 µg of high-quality total RNA from multiple sample types – all with one kit!For use with blood, cells and tissuesAlso works with tough to lyse samples (bacteria, yeast, plant)Effectively purifies total RNA of all sizes, including small RNAs >20 ntEfficient genomic DNA removal (column and DNase I-based)Contains Proteinase K for processing of tissues and blood samplesIncludes RNA Protection Reagent for sample preservationExcellent valueKit components available separately | New England Biolabs | https://www.neb.com/protocols/2017/11/28/quick-protocol-for-monarch-total-rna-miniprep-kit-neb-t2010 | 1 | 2018 | New England Biolabs 2018. Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010). protocols.io https://dx.doi.org/10.17504/protocols.io.nyadfse | 2021-04-15 09:15:35 | ||
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Single-temperature Double Digest Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cjhuj5 | New England Biolabs (NEB) | This is the Double Digest Protocol with Standard Restriction Enzymes, using a common reaction and same incubation temperature for both enzymes. | New England Biolabs | https://www.neb.com/protocols/2014/05/07/double-digest-protocol-with-standard-restriction-enzymes | 1 | 2014 | New England Biolabs 2014. Single-temperature Double Digest. protocols.io https://dx.doi.org/10.17504/protocols.io.cjhuj5 | 2021-04-15 09:15:09 | ||
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RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.be93jh8n | New England Biolabs (NEB), Coronavirus Method Development Community | This protocol utilizes the Monarch Total RNA Miniprep Kit to purify RNA from buccal swabs, nasopharyngeal samples, and saliva. | New England Biolabs | https://www.neb.com/protocols/2020/03/11/rna-purification-from-buccal-swabs-using-the-monarch-total-rna-miniprep-kit-neb-t2010 | 1 | 2020 | New England Biolabs 2020. RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit. protocols.io https://dx.doi.org/10.17504/protocols.io.be93jh8n | 2021-04-15 09:15:10 | ||
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Mixture for M0273 Taq PCR Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cicuav | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/taq-dna-polymerase-with-standard-taq-buffer-m0273 | 1 | 2015 | New England Biolabs 2015. Mixture for M0273 Taq PCR. protocols.io https://dx.doi.org/10.17504/protocols.io.cicuav | 2021-04-15 09:15:37 | |||
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10X RT Buffer Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cjwupd | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/first-strand-synthesis-protocol-with-reverse-transcriptase | 1 | 2014 | New England Biolabs 2014. 10X RT Buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.cjwupd | 2021-04-15 09:15:12 | |||
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PNGase F Mixture 2 Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cqhvt5 | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2014/07/31/pngase-f-protocol | 1 | 2015 | New England Biolabs 2015. PNGase F Mixture 2. protocols.io https://dx.doi.org/10.17504/protocols.io.cqhvt5 | 2021-04-15 09:15:14 | |||
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Gibson Assembly® Protocol (E5510) Resource Report Resource Website |
New England Biolabs | DOI:10.17504/protocols.io.cdms45 | New England Biolabs (NEB) | This is the protocol for the Gibson Assembly using the Gibson Assembly® Cloning Kit (E5510). | New England Biolabs | https://www.neb.com/protocols/2012/12/11/gibson-assembly-protocol-e5510 | 1 | 2015 | New England Biolabs 2015. Gibson Assembly® Protocol (E5510). protocols.io https://dx.doi.org/10.17504/protocols.io.cdms45 | 2021-04-15 09:15:25 |
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