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Fig 9. Effect of installation distance of shakers on weight on bit and accelerations of the drill-string. A. Change of weight on bit in the bottom hole over time. B. Maximum acceleration along the drill-string. C. Root mean square acceleration along the dr Resource Report Resource Website |
Peng Wang, Hongjian Ni, Ruihe Wang | 10.17504/protocols.io.kfactie | Wang P, Ni H, Wang R (2018) A new drilling method—Earthworm-like vibration drilling. PLoS ONE 13(4): e0194582. doi: 10.1371/journal.pone.0194582 | China University of Petroleum, China University of Petroleum, China University of Petroleum | https://doi.org/10.1371/journal.pone.0194582 | 1 | 2018 | Peng Wang, Hongjian Ni, Ruihe Wang 2018. Fig 9. Effect of installation distance of shakers on weight on bit and accelerations of the drill-string. A. Change of weight on bit in the bottom hole over time. B. Maximum acceleration along the drill-string. C. Root mean square acceleration along the dr. protocols.io dx.doi.org/10.17504/protocols.io.kfactie | 2021-03-29 03:08:38 | |||
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U Mass - Insulin clearance Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.x2wfqfe | Mouse Metabolic Phenotyping Centers | Summary: Insulin clearance test measure systemic clearance of insulin following a bolus injection. Liver accounts for the majority of systemic insulin clearance following secretion from pancreatic β- cells into portal circulation. Hepatic clearance of insulin may be affected by obesity and in other mouse models of altered metabolism. Alterations in insulin clearance may also affect glucose and lipid metabolism. | RRID:AB_2792981 | University of Massachusetts | http://mmpc.org/shared/document.aspx?id=140&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Insulin clearance. protocols.io dx.doi.org/10.17504/protocols.io.x2wfqfe | 2021-03-29 03:08:38 | |
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Around-the-horn PCR and cloning Resource Report Resource Website |
Stephen Floor | 10.17504/protocols.io.rf2d3qe | Stephen Floor Lab | This protocol is designed for 'around-the-horn' or 'divergent' PCR, where primers go around most or all of a plasmid but are pointed away from each other so they generate a linear product. Note that this protocol is written for Q5 polymerase, but works fine with other polymerases. To switch polymerases, just change the PCR reaction setup. | UCSF | 1 | 2018 | Stephen Floor 2018. Around-the-horn PCR and cloning. protocols.io dx.doi.org/10.17504/protocols.io.rf2d3qe | 2021-03-29 03:08:39 | |||
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Transient transfection of unicellular relative to animals Corallochytrium limacisporum with electroporation using Neon SystemVersion 1 Resource Report Resource Website |
Maria Rubio-Brotons | 10.17504/protocols.io.hmwb47e | Protist Research to Optimize Tools in Genetics (PROT-G), Multicellgenomelab | IBE CSIC-UPF | 1 | 2017 | Maria Rubio-Brotons 2017. Transient transfection of unicellular relative to animals Corallochytrium limacisporum with electroporation using Neon SystemVersion 1. protocols.io dx.doi.org/10.17504/protocols.io.hmwb47e | 2021-03-29 03:08:39 | ||||
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Derivitization of polyphosphate with cystamine to facilitate secondary labeling with biotin Resource Report Resource Website |
Catherine J. Baker, Stephanie A. Smith, James H. Morrissey | 10.17504/protocols.io.7z3hp8n | Investigation of the biological roles of inorganic polyphosphate has been facilitated by our previous development of a carbodiimide-based method for covalently coupling primary amine-containing molecules to the terminal phosphates of polyphosphate (Choi et al., Biochemistry 49:9935, 2010). We now extend that approach by using readily available “bridging molecules” containing a primary amine and an additional reactive moiety, including another primary amine, a thiol or a click chemistry reagent such as dibenzocyclooctyne. This two-step labeling method is used to covalently attach commercially available derivatives of biotin, peptide epitope tags, and fluorescent dyes to the ends of polyphosphate. This protocol specifically describes the labeling of heterogenous long-chain polyP with cystamine and the subsequent addition of maleimide-biotin. | Baker CJ, Smith SA, Morrissey JH (2020) Diversification of polyphosphate end-labeling via bridging molecules. PLoS ONE 15(8): e0237849. doi: 10.1371/journal.pone.0237849 | University of Michigan- Ann Arbor, University of Michigan- Ann Arbor, University of Michigan- Ann Arbor | https://doi.org/10.1371/journal.pone.0237849 | 1 | 2020 | Catherine J. Baker, Stephanie A. Smith, James H. Morrissey 2020. Derivitization of polyphosphate with cystamine to facilitate secondary labeling with biotin. protocols.io dx.doi.org/10.17504/protocols.io.7z3hp8n | 2021-03-29 03:08:39 | ||
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Expression and purification of (GST-tagged) (Kai) proteins Resource Report Resource Website |
Anika Wiegard | 10.17504/protocols.io.k68czhw | Axmann Lab | This protocol can be used for:(i) heterologous expression of GST-tagged proteins from pGEX-6P1 based expression vectors in E. coli.(ii) purification of recombinant proteins via affinity chromatography using glutathione-agarose or glutathione-sepharose (GST tagged protein can be eluted with glutathione. Alternatively, the tag can be cleaved off by prescission protease)(iii) further purification of the eluted protein via anion exchange chromatographyThis protocol was modified fromWiegard A, Dörrich AK, Deinzer HT, Beck C, Wilde A, Holtzendorff J, Axmann IM: Biochemical analysis of three putative KaiC clock proteins from Synechocystis sp. PCC 6803 suggests their functional divergence. Microbiology 2013, 159, 948-958Snijder J, Schuller JM, Wiegard A, Lössel, P, Schmelling NM, Axmann IM, Plitzko JM, Förster F, Heck AJR: Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state. Science 2017, 355(6330):1181-1184 | Institute for Synthetic Microbiology, CEPLAS, Heinrich Heine University Duesseldorf, Germany | 1 | 2018 | Anika Wiegard 2018. Expression and purification of (GST-tagged) (Kai) proteins. protocols.io dx.doi.org/10.17504/protocols.io.k68czhw | 2021-03-29 03:08:39 | |||
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Simulating colonic tissue mechanics using a structure-based material model in Abaqus Resource Report Resource Website |
Bhavesh Patel | 10.17504/protocols.io.wzeff3e | The structure-based material constitutive model typically used for the colon tissue (DOI:10.1016/j.jmbbm.2017.08.031, DOI:10.1016/j.jmbbm.2013.02.016) is not readily available in the Finite Element software Abaqus, which is commonly used for biomechanical simuations. In this protocol, we provide step-by-step guidelines to use such material model in Abaqus using the User Subroutine implemented by the autor. | California Medical Innovations Institute | 1 | 2020 | Bhavesh Patel 2020. Simulating colonic tissue mechanics using a structure-based material model in Abaqus. protocols.io dx.doi.org/10.17504/protocols.io.wzeff3e | 2021-03-29 03:08:39 | ||||
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Background and Rationale (Part 1 of Phase 3 study of Vaccine Candidate for COVID-19) Resource Report Resource Website |
Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores | 10.17504/protocols.io.bj5tkq6n | Coronavirus Method Development Community | This is a collection of protocols for: "Phase 3 randomized, double-blinded, placebo-controlled trial to evaluate the safety, immunogenicity, and efficacy of Vaccine Candidate against COVID-19 in adults ≥ 18 years of age"This generic Phase 3 protocol was developed by the PATH team with support of the Bill and Melinda Gates Foundation. The aim of the collection is to share recommended best practices in designing and implementing a Phase 3 study of a COVID-19 vaccine candidate. As Phase 3 trials of different Vaccine Candidates proceed around the world, following the same protocols will ensure consistency and comparability of the Phase 3 trial results.Please note that this is an evolving document, to be versioned and updated, based on community feedback and new data. | Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington) | 1 | 2020 | Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores 2020. Background and Rationale (Part 1 of Phase 3 study of Vaccine Candidate for COVID-19). protocols.io dx.doi.org/10.17504/protocols.io.bj5tkq6n | 2021-03-29 03:08:36 | |||
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RDH Resource Report Resource Website |
Irfan Uddin, Taeeb Ahmad, Furqan Aziz | 10.17504/protocols.io.bdyzi7x6 | This algorithm implements Reversible Data Hiding (RDH) technique by rearranging the columns (or rows) of the image in a way that enhances the smooth regions of an image. Any difference based technique to embed data can then be used in the transformed image. | Aziz F, Ahmad T, Malik AH, Uddin MI, Ahmad S, Sharaf M (2020) Reversible data hiding techniques with high message embedding capacity in images. PLoS ONE 15(5): e0231602. doi: 10.1371/journal.pone.0231602 | Kohat University of Science and Technology (KUST), Institute of Management Sciences, Peshawar, Institute of Management Sciences, Peshawar | https://github.com/mirfanud/datahiding | 1 | 2020 | Irfan Uddin, Taeeb Ahmad, Furqan Aziz 2020. RDH. protocols.io dx.doi.org/10.17504/protocols.io.bdyzi7x6 | 2021-03-29 03:08:36 | ||
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Script R15: CoNet Analysis - Formatting Relative Abundance Files Resource Report Resource Website |
HANNIGAN GD, GRICE EA, ET AL. | 10.17504/protocols.io.ejpbcmn | VERVE Net, Club Grice | This protocol outlines the analysis used to generate input files for CoNet for the phage-bacteria network. Based on methods from the following publication:Hannigan, Geoffrey D., et al. "The Human Skin Double-Stranded DNA Virome: Topographical and Temporal Diversity, Genetic Enrichment, and Dynamic Associations with the Host Microbiome." mBio 6.5 (2015): e01578-15. | Kindler L, Stoliartchouk A, Teytelman L, Hurwitz BL, Method-centered digital communities on protocols.io for fast-paced scientific innovation. F1000Research doi: 10.12688/f1000research.9453.2 | DEPARTMENT OF DERMATOLOGY UNIVERSITY OF PENNSYLVANIA, DEPARTMENT OF DERMATOLOGY UNIVERSITY OF PENNSYLVANIA, DEPARTMENT OF DERMATOLOGY UNIVERSITY OF PENNSYLVANIA | http://mbio.asm.org/content/6/5/e01578-15.full | 1 | 2016 | HANNIGAN GD, GRICE EA, ET AL. 2016. Script R15: CoNet Analysis - Formatting Relative Abundance Files. protocols.io dx.doi.org/10.17504/protocols.io.ejpbcmn | 2021-03-29 03:08:36 | |
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10X-CITEseq protocol (COVID-19 patient samples +/- tetramer stain) Resource Report Resource Website |
Yang Sun, David Lee, George Hartoularos, Jimmie Ye | 10.17504/protocols.io.bqnqmvdw | Coronavirus Method Development Community | PurposeTo detail the workflow for scCITE-Seq of 24 PBMC samples with 2 technicians. This is based on internal 10X CITEseq protocol v9. | UCSF, UCSF, UCSF, UCSF | 1 | 2021 | Yang Sun, David Lee, George Hartoularos, Jimmie Ye 2021. 10X-CITEseq protocol (COVID-19 patient samples +/- tetramer stain). protocols.io dx.doi.org/10.17504/protocols.io.bqnqmvdw | 2021-03-29 03:08:37 | |||
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Virus purification protocol for Ehv-163 and other viruses. Resource Report Resource Website |
Elena Yakubovskaya, Tatiana Zaliznyak, Joaquin Martinez Martinez, Gordon Taylor | 10.17504/protocols.io.bcpvivn6 | TaylorLab | Reliable protocol for virus purification is an essential step for characterization and identification of individual virus particles in order to obtain information on biochemical and physical properties of the virus and for studying interactions between host and virus at the single-cell level. The purified virus should be physically and chemically undamaged by the purification procedure and free from contaminating host-material. To obtain high-quality EhV sample (the virus that infecta coccolithophorid, Emiliania huxleyi) sutable for AFM, Raman and CryoEM analysis, we modified the protocol published by Lawrence and Steward (“Purification of viruses by centrifugation” J. E. Lawrence and G. F. Steward; MAVE Chapter 17, 2010, 166–181 © 2010, by the American Society of Limnology and Oceanography, Inc.). | School of Marine & Atmospheric Sciences, Stony Brook University, School of Marine & Atmospheric Sciences, Stony Brook University, Bigelow Laboratory for Ocean Sciences, School of Marine & Atmospheric Sciences, Stony Brook University | http://www.soest.hawaii.edu/oceanography/faculty/steward/StewardLab/Projects_files/Lawrence%26Steward2010_MAVE.pdf | 1 | 2020 | Elena Yakubovskaya, Tatiana Zaliznyak, Joaquin Martinez Martinez, Gordon Taylor 2020. Virus purification protocol for Ehv-163 and other viruses.. protocols.io dx.doi.org/10.17504/protocols.io.bcpvivn6 | 2021-03-29 03:08:37 | ||
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Pellet resuspension Resource Report Resource Website |
André M. Comeau and Rachel T. Noble | 10.17504/protocols.io.dzt76m | VERVE Net | For use in Viral and bacterial isolates, propagation and preparation of stocks. | Manual of Aquatic Viral Ecology | http://www.aslo.org/books/mave/MAVE_019.pdf | 1 | 2016 | André M. Comeau and Rachel T. Noble 2016. Pellet resuspension. protocols.io dx.doi.org/10.17504/protocols.io.dzt76m | 2021-03-29 03:08:37 | ||
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E. coli K12 DNA Extraction Resource Report Resource Website |
Kenneth Schackart, Kattika Kaarj | 10.17504/protocols.io.yujfwun | 481b Laboratory | How to extract DNA from E. coli K12 using Wizard® Genomic DNA Purification Kit by Promega®.I do not claim any credit for the development of this protocol. It has been adapted from the protocol detailed in: . | University of Arizona, University of Arizona | 1 | 2019 | Kenneth Schackart, Kattika Kaarj 2019. E. coli K12 DNA Extraction. protocols.io dx.doi.org/10.17504/protocols.io.yujfwun | 2021-03-29 03:08:37 | |||
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Using sequins in metagenome experiments. Resource Report Resource Website |
Tim Mercer | 10.17504/protocols.io.bic3kayn | Metagenome sequins are a set of synthetic DNA controls that reflect the sequence complexity, GC content, phylogenetic diversity and abundance of a natural microbial community. The sequins are ‘spiked-in’ to your DNA sample, which together undergo to library preparation, sequencing and analysis. The sequins can then be distinguished from you sample DNA in the output library by their synthetic sequence, and analyzed as internal controls. Sequins are compatible with all standards library preparation and sequencing methods. This protocol describes the laboratory steps required to re-suspend and spike the sequins into your DNA sample, as well as the bioinformatic steps required to analyze sequins in your output library. For further details on the design, validation and use of sequins, we refer users to ‘Synthetic microbe communities provide internal reference standards for metagenome sequencing and analysis’ by Hardwick et. al., (2018) Nature Communications. | Garvan Institute of Medical Research | http://www.sequinstandards.com | 1 | 2020 | Tim Mercer 2020. Using sequins in metagenome experiments.. protocols.io dx.doi.org/10.17504/protocols.io.bic3kayn | 2021-03-29 03:08:37 | |||
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High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing Resource Report Resource Website |
Ashley Jones, Cynthia Torkel, David Stanley, Jamila Nasim, Justin Borevitz, Benjamin Schwessinger | 10.17504/protocols.io.bss7nehn | High molecular weight DNA extraction from all kingdoms | Rapid advancements in long-read sequencing technologies have transformed sequencing read lengths from bps to Mbps, which has enabled chromosome-scale genome assemblies. However, read lengths are now becoming limited by the extraction of pure high-molecular weight DNA suitable for long-read sequencing, which is particularly challenging in plants and fungi. To overcome this, we present a protocol collection; high-molecular weight DNA extraction, clean-up and size selection for long-read sequencing. We optimised a gentle magnetic bead based high-molecular weight DNA extraction, which is presented here in detail. The protocol circumvents spin columns and high-centrifugation, to limit DNA fragmentation. The protocol is scalable based on tissue input, which can be used on many species of plants, fungi, reptiles, insects and bacteria. It is also cost effective compared to kit-based protocols and hence applicable at scale at low resource settings. An optional sorbitol wash is listed and is highly recommended for plant and fungal tissues. To further remove any remaining contaminants such as phenols and polysaccharides, optional DNA clean-up and size selection strategies are given. This protocol collection is suitable for all common long-read sequencing platforms, such as technologies offered by PacBio and Nanopore. Using these protocols, sequencing on the Oxford Nanopore MinION can achieve read length N50 values of 30-50 kb, with reads exceeding 200 kb and outputs ranging from 15-30 Gbp. This has been routinely achieved with eucalypts, acacias, rice, themeda, wheat, wheat rusts, various other fungi, geckos, skinks, ticks, ladybird beetles, caterpillars and E. coli. | Research School of Biology, Australian National University, Canberra, ACT, Australia, Research School of Biology, Australian National University, Canberra, ACT, Australia, Research School of Biology, Australian National University, Canberra, ACT, Australia, Research School of Biology, Australian National University, Canberra, ACT, Australia, Research School of Biology, Australian National University, Canberra, ACT, Australia, Research School of Biology, Australian National University, Canberra, ACT, Australia | 1 | 2021 | Ashley Jones, Cynthia Torkel, David Stanley, Jamila Nasim, Justin Borevitz, Benjamin Schwessinger 2021. High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing. protocols.io dx.doi.org/10.17504/protocols.io.bss7nehn | 2021-03-29 03:08:37 | |||
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Protocol for Subculture of Differentiated Blood-Brain Barrier Endothelial Cells onto Plates and Filters Resource Report Resource Website |
Ethan Lippmann, Hannah Wilson, Emma Neal | 10.17504/protocols.io.8g5hty6 | Neurodegeneration Method Development Community | Department of Chemical Engineering, Vanderbilt University, Nashville, TN, USA, Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA, Department of Chemical Engineering, Vanderbilt University, Nashville, TN, USA | 1 | 2020 | Ethan Lippmann, Hannah Wilson, Emma Neal 2020. Protocol for Subculture of Differentiated Blood-Brain Barrier Endothelial Cells onto Plates and Filters. protocols.io dx.doi.org/10.17504/protocols.io.8g5hty6 | 2021-03-29 03:08:37 | ||||
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SARS-CoV-2 McGill Nextera Flex sequencing protocol_SS_V3_LA1_5uLRT Resource Report Resource Website |
Sarah Reiling, Marie-Michelle Simon, Anne-Marie Roy, Shu-Huang Chen, Josh Quick, Ioannis Ragoussis | 10.17504/protocols.io.bisbkean | Coronavirus Method Development Community, McGill Genome Centre | How the Nextera DNA Flex Assay Works The Nextera DNA Flex library prep kit uses a bead-based transposome complex to tagment genomic DNA, which is a process that fragments DNA and then tags the DNA with adapter sequences in one step. After it is saturated with input DNA, the bead-based transposome complex fragments a set number of DNA molecules. This fragmentation provides flexibility to use a wide DNA input range to generate normalized libraries of consistent tight fragment size distribution. Following tagmentation, a limited-cycle PCR adds Nextera DNA Flex-specific index adapter sequences to the ends of a DNA fragment. This step enables capability across all Illumina sequencing platforms. A subsequent Sample Purification Beads (SPB) cleanup step then purifies libraries for use on an Illumina sequencer. The double-stranded DNA library is denatured before hybridization of the biotin probe oligonucleotide pool. PCR Amplicons for Nextera FlexWhen starting with PCR amplicons, the PCR amplicon must be > 150 bp. The standard clean up protocol depletes libraries Therefore, Illumina recommends that amplicons Shorter amplicons can otherwise be lost during the library cleanup step. Tagmentation cannot add an adapter directly to the distal end of a fragment, so a drop in sequencing coverage of ~50 bp from each distal end is expected. To ensure sufficient coverage of the amplicon target region, design primers to extend beyond the target region by 50 bp per end. More information can be found here: https://emea.support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_nextera/nextera_dna_flex/nextera-dna-flex-library-prep-reference-guide-1000000025416-07.pdf | McGill University, McGill University, McGill University, McGill University, University of Birmingham, McGill University | 1 | 2020 | Sarah Reiling, Marie-Michelle Simon, Anne-Marie Roy, Shu-Huang Chen, Josh Quick, Ioannis Ragoussis 2020. SARS-CoV-2 McGill Nextera Flex sequencing protocol_SS_V3_LA1_5uLRT. protocols.io dx.doi.org/10.17504/protocols.io.bisbkean | 2021-03-29 03:08:37 | |||
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Influenza B virus YAMAGATA lineage TaqMan 2018 / FluB-YAMA-TM2018 Resource Report Resource Website |
Ian Mackay, Judy Northill | 10.17504/protocols.io.rdid24e | Public Health Virology, Forensic and Scientific Services | This protocol was designed and developed at this laboratory. The protocol specifically aims to amplify strains of Influenza B YAMAGATA virus lineage and not strains of the VICTORIA virus lineage or other virus species. The assay targets the haemagglutinin (HA) region and is designed as a qualitative lineage-typing test for human cases of seasonal influenza virus type B infections. FluB-YAMA-TM2018 is ideally used alongside its companion protocol, "Influenza B virus VICTORIA lineage TaqMan 2018" (FluB-VICT-TM2018), which aims to target influenza B virus VICTORIA lineage strains exclusively. The two assays perform best as UNIPLEX protocols; a drop in sensitivity was observed when combined in a DUPLEX format. | Public Health Virology, Forensic and Scientific Services, Public Health Virology, Forensic and Scientific Services | 1 | 2018 | Ian Mackay, Judy Northill 2018. Influenza B virus YAMAGATA lineage TaqMan 2018 / FluB-YAMA-TM2018. protocols.io dx.doi.org/10.17504/protocols.io.rdid24e | 2021-03-29 03:08:37 | |||
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XPRIZE SHINE - Paper-based SARS-CoV-2 Saliva Test Resource Report Resource Website |
Jon Arizti-Sanz, Catherine A. Freije, Chloe K. Boehm, Sameed M. Siddiqui, Allen M. Goodman, Tinna-Solveig F. Kosoko-Thoroddsen, A'Doriann Y. Bradley, Jeremy Johnson, Pardis C. Sabeti, Cameron Myhrvold | 10.17504/protocols.io.bk2fkybn | XPRIZE Rapid Covid Testing | This protocol describes how to perform a SHINE paper-based assay to detect SARS-CoV-2 RNA from a self-collected saliva sample. This protocol is intended for in-home use. All enzymatic components are provided as a single-test freeze-dried pellet for shelf-stable storage, and all steps of the protocol are performed at ambient temperature. The protocol presented here is an improved version of the method presented in Arizti-Sanz J*, Freije CA*, et al. Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2. bioRxiv (2020). | Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Harvard-MIT Program in Health Sciences and Technology, 77 Massachusetts Ave., Cambridge, MA 02139, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Computational and Systems Biology PhD Program, MIT, Cambridge, MA 02139, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Harvard T.H. Chan School of Public Health, 677 Huntington Avenue, Boston, MA 02115, USA.; Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA.; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA. | 1 | 2020 | Jon Arizti-Sanz, Catherine A. Freije, Chloe K. Boehm, Sameed M. Siddiqui, Allen M. Goodman, Tinna-Solveig F. Kosoko-Thoroddsen, A'Doriann Y. Bradley, Jeremy Johnson, Pardis C. Sabeti, Cameron Myhrvold 2020. XPRIZE SHINE - Paper-based SARS-CoV-2 Saliva Test. protocols.io dx.doi.org/10.17504/protocols.io.bk2fkybn | 2021-03-29 03:08:37 |
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