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Name Authors DOI Group Summary Associated Publications RRIDs used Affiliations External URL Version Publication Date Proper Citation Record Last Update
Lessons learned from the resilience of public health systems, hospitals and their personnel to the COVID-19 pandemic: a scoping review protocol.
 
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Lola Traverson, Isadora Mathevet, Amanda Paes, Karla Paz, Andrea Andrade, Katarina Ost, Kate Zinszer, Valery Ridde 10.17504/protocols.io.bikwkcxe CEPED / IRD / Université de Paris, CEPED / IRD / Université de Paris, Fiocruz-PE, Fiocruz-PE, Fiocruz-PE, Université de Montréal, Université de Montréal, CEPED / IRD / Université de Paris 1 2020 Lola Traverson, Isadora Mathevet, Amanda Paes, Karla Paz, Andrea Andrade, Katarina Ost, Kate Zinszer, Valery Ridde 2020. Lessons learned from the resilience of public health systems, hospitals and their personnel to the COVID-19 pandemic: a scoping review protocol.. protocols.io dx.doi.org/10.17504/protocols.io.bikwkcxe 2021-03-29 03:08:31
Whole-body clearing of beetles by successive treatment of hydrogen peroxide and CUBIC reagents
 
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Monto Kuroda, Shinya Kuroda 10.17504/protocols.io.ba33igqn Optical Clearing of Tissue Internal tissues of multicellular organisms cannot directly be seen because they contain pigments. For this reason, whole-body clearing methods have been developed and applied to mammals such as mice. Insects such as beetles, however, cannot be cleared by the mammalian method because of pigments such as melanin in their exoskeletons. In this study, we tried to develop a whole-body clearing method for large beetles. We first bleached the exoskeleton using a hydrogen peroxide treatment, and applied the the advanced Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis (CUBIC) reagents to make the internal tissues transparent. The combined method of hydrogen peroxide and the advanced CUBIC allowed us to successfully perform whole-body clearing of the large beetles. Keika High school, l, 5-6-6 Hakusan, Bunkyo-ku, 112-8612, Tokyo, Japan, Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan 1 2020 Monto Kuroda, Shinya Kuroda 2020. Whole-body clearing of beetles by successive treatment of hydrogen peroxide and CUBIC reagents. protocols.io dx.doi.org/10.17504/protocols.io.ba33igqn 2021-03-29 03:08:31
Root Growth and Spatial Distribution Characteristics for Seedlings raised in Substrate and Transplanted Cotton
 
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Xiaoyu Zhi, Yabing Li, Yingchun Han, Lu Feng 10.17504/protocols.io.js4cngw Zhi X, Han Y, Li Y, Wang G, Feng L, Yang B, Fan Z, Lei Y, Du W, Mao S (2017) Root growth and spatial distribution characteristics for seedlings raised in substrate and transplanted cotton. PLoS ONE 12(12): e0190032. doi: 10.1371/journal.pone.0190032 Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences https://doi.org/10.1371/journal.pone.0190032 1 2017 Xiaoyu Zhi, Yabing Li, Yingchun Han, Lu Feng 2017. Root Growth and Spatial Distribution Characteristics for Seedlings raised in Substrate and Transplanted Cotton. protocols.io dx.doi.org/10.17504/protocols.io.js4cngw 2021-03-29 03:08:31
Sandwich ELISA Protocol
 
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Sam Li 10.17504/protocols.io.98xh9xn BioLegend BioLegend https://www.biolegend.com/protocols/sandwich-elisa-protocol/4268/ 3 2019 Sam Li 2019. Sandwich ELISA Protocol. protocols.io dx.doi.org/10.17504/protocols.io.98xh9xn 2021-03-29 03:08:30
Glycerol Stock
 
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Alan J. Cone 10.17504/protocols.io.dbe2jd Ju Lab Method to create a long-term stable store of organisms. Especially useful if you want to store already transformed versions of organisms to aid in future experiments. Wright State University 1 2015 Alan J. Cone 2015. Glycerol Stock. protocols.io dx.doi.org/10.17504/protocols.io.dbe2jd 2021-03-29 03:08:30
Collection and Post-Surgical Excision of Human Kidney Tissue through the Cooperative Human Tissue Network
 
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Maya Brewer, Jamie Allen, Elizabeth Neumann, Agnes Fogo, Raymond Harris, Danielle Gutierrez, Mark De Caestecker, Jeff Spraggins 10.17504/protocols.io.7gehjte VU Biomolecular Multimodal Imaging Center, Human BioMolecular Atlas Program (HuBMAP) Method Development Community Scope:Obtain kidney tissue and metadata about tissue location within the whole kidney for storage and analysis.Expected Outcome:A portion of kidney tissue and a series of images that provide information about the original location of the smaller tissue. Vanderbilt University, Vanderbilt University, Vanderbilt University, Vanderbilt University Medical Center, Vanderbilt University Medical Center, Vanderbilt University, Division of Nephrology, Vanderbilt University Medical Center, Vanderbilt University 1 2019 Maya Brewer, Jamie Allen, Elizabeth Neumann, Agnes Fogo, Raymond Harris, Danielle Gutierrez, Mark De Caestecker, Jeff Spraggins 2019. Collection and Post-Surgical Excision of Human Kidney Tissue through the Cooperative Human Tissue Network. protocols.io dx.doi.org/10.17504/protocols.io.7gehjte 2021-03-29 03:08:31
QUESTIONNAIRE ABOUT ECO METHOD IMPLEMENTATION IN THE UNIVERSITY
 
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Juan Jesus Torres-Gordillo 10.17504/protocols.io.bhp5j5q6 The purpose of this questionnaire is to know the impact of the implementation of the ECO method in the University. It is part of an innovation project, entitled ECO in Higher Education. Teaching inspired by the environment, funded by the University of Seville's 3rd Teaching Plan. It is intended for teachers who have been part of the innovation project. The data collected will be analysed to be used in the final report, as well as in some conference communications or journal articles.The questionnaire consists of 29 questions, divided into three blocks: 12 initial questions on demographic data, 9 closed-ended questions with four options and 8 open-ended questions. For open-ended questions, we ask that you answer in detail and as explicitly as possible.Each teacher is requested to complete one questionnaire per course in which they have implemented the ECO method. Similarly, if there have been two teachers in a shared course, each must fulfill, from their experience, a separate questionnaire.Thank you very much for your collaboration.Prof. Juan-Jesús Torres-GordilloUniversity of Seville (Spain) Torres-Gordillo J, Melero-Aguilar N, García-Jiménez J (2020) Improving the university teaching-learning process with ECO methodology: Teachers’ perceptions. PLoS ONE 15(8): e0237712. doi: 10.1371/journal.pone.0237712 Universidad de Sevilla https://doi.org/10.1371/journal.pone.0237712 1 2020 Juan Jesus Torres-Gordillo 2020. QUESTIONNAIRE ABOUT ECO METHOD IMPLEMENTATION IN THE UNIVERSITY. protocols.io dx.doi.org/10.17504/protocols.io.bhp5j5q6 2021-03-29 03:08:31
PNGase F Mixture 1
 
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New England Biolabs 10.17504/protocols.io.cqgvtv New England Biolabs (NEB) New England Biolabs https://www.neb.com/protocols/2014/07/31/pngase-f-protocol 1 2015 New England Biolabs 2015. PNGase F Mixture 1. protocols.io dx.doi.org/10.17504/protocols.io.cqgvtv 2021-03-29 03:08:31
BIOL 354W - Research Methods in Advance Microbiology
 
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Rosa Leon 10.17504/protocols.io.m3dc8i6 Leon Zayas Lab This protocol series will guide students through the experience of analyzing metagenomic data.  Willamette University 8 2018 Rosa Leon 2018. BIOL 354W - Research Methods in Advance Microbiology. protocols.io dx.doi.org/10.17504/protocols.io.m3dc8i6 2021-03-29 03:08:31
Protocols for Agarose Preparation Using Denville LE Agarose
 
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Denville Scientific 10.17504/protocols.io.gskbwcw Denville Scientific, Inc. Denville LE Agarose is an all purpose agarose for routine nucleic acid electrophoresis of fragments between 500bp-23,000 bp.Denville LE Agarose has no detectable DNase or RNase activity.Please refer to the appropriate protocol below, depending on whether you are using a hot plate or a microwave. https://www.denvillescientific.com/products/agarose 1 2016 Denville Scientific 2016. Protocols for Agarose Preparation Using Denville LE Agarose. protocols.io dx.doi.org/10.17504/protocols.io.gskbwcw 2021-03-29 03:08:31
Isolate prokaryotes from sponge tissue (SAP)
 
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Martin Thomas Jahn 10.17504/protocols.io.u8vezw6 hostmicrobeprotocols Protocoll to create a fixed suspension of sponge associated prokaryotes (SAP) from sponge tissue.This can serve as the basis for Fluorescence in situ hybridisation and/or cell sorting.This protocol was tested for different sponge species but might also be adapted to other organisms (let others know)The protocol was modified from :Fieseler L, Horn M, Wagner M, Hentschel U. (2006) Discovery of the novel candidate phylum "Potibactetia" in marine sponges (vol 70, pg 3724, 2004). Applied and Environmental Microbiology;72(8):5677-.PMID:15184179 DOI:10.1128/AEM.70.6.3724-3732.2004 CRC1182 1 2019 Martin Thomas Jahn 2019. Isolate prokaryotes from sponge tissue (SAP). protocols.io dx.doi.org/10.17504/protocols.io.u8vezw6 2021-03-29 03:08:31
NS-Forest version 2
 
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Brian Aevermann, Richard Scheuermann 10.17504/protocols.io.un7evhn Human Cell Atlas Method Development Community NS-Forest is an alogrithm that determines the minimum set of genes that are necessary and sufficient to define a cell type cluster derived from single cell RNAseq expression data. Development and stable releases can be found at : https://github.com/JCVenterInstitute/NSForest J. Craig Venter Institute, J. Craig Venter Institute 1 2018 Brian Aevermann, Richard Scheuermann 2018. NS-Forest version 2. protocols.io dx.doi.org/10.17504/protocols.io.un7evhn 2021-03-29 03:08:32
Yale - Creatine Kinase Activity
 
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John Stack, Gary Cline 10.17504/protocols.io.y3sfyne Mouse Metabolic Phenotyping Centers Procedure used to determine the creatine kinase activity in blood , serum, and plasma. Creatine kinase activity is measured by the enzymatically coupled reactions of creatine kinase, hexokinase, and glucose-6-P dehydrogenase. The rate of NADPH formation is monitored by the change in absorbance at 340 nm. Yale University, Yale University https://mmpc.org/shared/document.aspx?id=219&docType=Protocol 1 2019 John Stack, Gary Cline 2019. Yale - Creatine Kinase Activity. protocols.io dx.doi.org/10.17504/protocols.io.y3sfyne 2021-03-29 03:08:32
Adapted protocol to extract total RNA using TRIzol® (Invitrogen, Thermo)
 
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Talita Gagliardi 10.17504/protocols.io.w8vfhw6 Total RNA extraction using TRizol reagent.Attention: for nasopharyngeal aspirates, we use regular TRizol reagent in this assay, but for blood samples it is better to use a special one as "TRizol LS". Matsuno AK, Gagliardi TB, Paula FE, Luna LKS, Jesus BLS, Stein RT, Aragon DC, Carlotti APCP, Arruda E (2019) Human coronavirus alone or in co-infection with rhinovirus C is a risk factor for severe respiratory disease and admission to the pediatric intensive care unit: A one-year study in Southeast Brazil. PLoS ONE 14(6): e0217744. doi: 10.1371/journal.pone.0217744 University of Maryland, College Park https://doi.org/10.1371/journal.pone.0217744 1 2019 Talita Gagliardi 2019. Adapted protocol to extract total RNA using TRIzol® (Invitrogen, Thermo). protocols.io dx.doi.org/10.17504/protocols.io.w8vfhw6 2021-03-29 03:08:32
Digestion BclI
 
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Antoine ZILLER 10.17504/protocols.io.ecvbaw6 Phd student 1 2016 Antoine ZILLER 2016. Digestion BclI. protocols.io dx.doi.org/10.17504/protocols.io.ecvbaw6 2021-03-29 03:08:31
Transient tranfection of unicellular relative of animals, Creolimax fragrantissima, using Lonza Nucleofector
 
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Aleksandra Kozyczkowska 10.17504/protocols.io.r65d9g6 Protist Research to Optimize Tools in Genetics (PROT-G), Multicellgenomelab, Emerging Organisms for Biology Institut of Evolutionary Biology (UPF-CSIC) 1 2018 Aleksandra Kozyczkowska 2018. Transient tranfection of unicellular relative of animals, Creolimax fragrantissima, using Lonza Nucleofector. protocols.io dx.doi.org/10.17504/protocols.io.r65d9g6 2021-03-29 03:08:32
Study Population (Part 4 of Phase 3 study of Vaccine Candidate for COVID-19)
 
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Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores 10.17504/protocols.io.bj5xkq7n Coronavirus Method Development Community This is Part 4 of "Phase 3 randomized, double-blinded, placebo-controlled trial to evaluate the safety, immunogenicity, and efficacy of Vaccine Candidate against COVID-19 in adults > 18 years of age"This generic Phase 3 protocol was developed by the PATH team with support of the Bill and Melinda Gates Foundation. The aim of the collection is to share recommended best practices in designing and implementing a Phase 3 study of a COVID-19 vaccine candidate. As Phase 3 trials of different Vaccine Candidates proceed around the world, following the same protocols will ensure consistency and comparability of the Phase 3 trial results.Please note that this is an evolving document, to be versioned and updated, based on community feedback and new data. Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington), Center for Vaccine Innovation and Access, PATH (Washington D.C. and Seattle, Washington) 1 2020 Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores 2020. Study Population (Part 4 of Phase 3 study of Vaccine Candidate for COVID-19). protocols.io dx.doi.org/10.17504/protocols.io.bj5xkq7n 2021-03-29 03:08:33
An analytical pipeline of assembly and annotation of the Betta splendens genome.
 
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Xin Liu 10.17504/protocols.io.qq9dvz6 GigaScience Press, BGI From here, You can learn about the detail methods of genome assembly and gene annotation of the Betta splendens genome. BGI-Shenzhen 1 2018 Xin Liu 2018. An analytical pipeline of assembly and annotation of the Betta splendens genome.. protocols.io dx.doi.org/10.17504/protocols.io.qq9dvz6 2021-03-29 03:08:30
Trace Metal Solution for ESAW Media
 
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Ashley Humphrey 10.17504/protocols.io.gdabs2e Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) Trace metals solution to be used in ESAW Media for Marine Phytoplankton  University of Tennessee, Knoxville 1 2016 Ashley Humphrey 2016. Trace Metal Solution for ESAW Media. protocols.io dx.doi.org/10.17504/protocols.io.gdabs2e 2021-03-29 03:08:33
MG_HW6: Gene Calls with Prodigal
 
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James Thornton 10.17504/protocols.io.f4ubqww Hurwitz Lab, MetaFunc Course This protocol provides the procedure to generate gene calls on your contigs using Prodigal.  Hurwitz Lab 2 2016 James Thornton 2016. MG_HW6: Gene Calls with Prodigal. protocols.io dx.doi.org/10.17504/protocols.io.f4ubqww 2021-03-29 03:08:33

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