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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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ENNOLIFE SARS-CoV-2 Antigen Test Kit Protocol Resource Report Resource Website |
Hsiao-Chung Tsai | DOI:10.17504/protocols.io.bke3ktgn | Coronavirus Method Development Community, ProtectLife-AnTaimmu, XPRIZE Rapid Covid Testing | General manager, ProtectLife International Biomedical Inc. | 1 | 2020 | Hsiao-Chung Tsai 2020. ENNOLIFE SARS-CoV-2 Antigen Test Kit Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bke3ktgn | 2021-04-15 09:15:50 | ||||
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Slot/Dot Blot protocol. Resource Report Resource Website |
beldenwj | DOI:10.17504/protocols.io.bk3mkyk6 | XPRIZE Rapid Covid Testing | This is a simple slot/dot blot protocol for detecting antigen on a membrane. Using the method outline here, one can achieve results in under 90 min because it eliminates the need for running a gel, transfering the protein in a transfer apparatus, blocking, secondary antibody incubation and the washes between primary and secondary antibodies. | Rutgers University | 1 | 2020 | beldenwj 2020. Slot/Dot Blot protocol.. protocols.io https://dx.doi.org/10.17504/protocols.io.bk3mkyk6 | 2021-04-15 09:15:37 | |||
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SARS-CoV-2 Antigen Detection ELISA Resource Report Resource Website |
Rob Parks, Barton Haynes | DOI:10.17504/protocols.io.bk2ckyaw | XPRIZE Rapid Covid Testing | Duke Human Vaccine Institute, Duke Human Vaccine Institute | 1 | 2020 | Rob Parks, Barton Haynes 2020. SARS-CoV-2 Antigen Detection ELISA. protocols.io https://dx.doi.org/10.17504/protocols.io.bk2ckyaw | 2021-04-15 09:15:49 | ||||
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XPRIZE SHINE - Paper-based SARS-CoV-2 Saliva Test Resource Report Resource Website |
Jon Arizti-Sanz, Catherine A. Freije, Chloe K. Boehm, Sameed M. Siddiqui, Allen M. Goodman, Tinna-Solveig F. Kosoko-Thoroddsen, A'Doriann Y. Bradley, Jeremy Johnson, Pardis C. Sabeti, Cameron Myhrvold | DOI:10.17504/protocols.io.bk2fkybn | XPRIZE Rapid Covid Testing | This protocol describes how to perform a SHINE paper-based assay to detect SARS-CoV-2 RNA from a self-collected saliva sample. This protocol is intended for in-home use. All enzymatic components are provided as a single-test freeze-dried pellet for shelf-stable storage, and all steps of the protocol are performed at ambient temperature. The protocol presented here is an improved version of the method presented in Arizti-Sanz J*, Freije CA*, et al. Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2. bioRxiv (2020). | Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Harvard-MIT Program in Health Sciences and Technology, 77 Massachusetts Ave., Cambridge, MA 02139, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Computational and Systems Biology PhD Program, MIT, Cambridge, MA 02139, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Harvard T.H. Chan School of Public Health, 677 Huntington Avenue, Boston, MA 02115, USA.; Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA.; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA. | 1 | 2020 | Jon Arizti-Sanz, Catherine A. Freije, Chloe K. Boehm, Sameed M. Siddiqui, Allen M. Goodman, Tinna-Solveig F. Kosoko-Thoroddsen, A'Doriann Y. Bradley, Jeremy Johnson, Pardis C. Sabeti, Cameron Myhrvold 2020. XPRIZE SHINE - Paper-based SARS-CoV-2 Saliva Test. protocols.io https://dx.doi.org/10.17504/protocols.io.bk2fkybn | 2021-04-15 09:15:57 | |||
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GM Covid-19 saliva test (v1) Resource Report Resource Website |
DOI:10.17504/protocols.io.bkgfkttn | Narwhale for XPrize, XPRIZE Rapid Covid Testing | GM Covid-19 saliva test is a RT-PCR test intended for qualitative detection of nucleic acids from SARS-CoV-2 in saliva specimen. The test is used for screening purpose under class of "research used only" (RUO). The test is not for diagnostic purpose. Positive result would be referred to an official clinical test with appropriate certification (i.e CLIA) required for clinical diagnosis. | GM Biosciences | 1 | 2020 | 2020. GM Covid-19 saliva test (v1). protocols.io https://dx.doi.org/10.17504/protocols.io.bkgfkttn | 2021-04-15 09:15:52 | ||||
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Sample preparation Resource Report Resource Website |
philippe.bechtold | DOI:10.17504/protocols.io.bjygkptw | XPRIZE Rapid Covid Testing | ETHZ - ETH Zurich | 1 | 2020 | philippe.bechtold 2020. Sample preparation. protocols.io https://dx.doi.org/10.17504/protocols.io.bjygkptw | 2021-04-15 09:15:50 | ||||
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Dawatek COVID protocol Resource Report Resource Website |
Leela Raavi, Davina Moossazadeh, Isaac Chadri | DOI:10.17504/protocols.io.bk24kygw | XPRIZE Rapid Covid Testing | Dawatek, Dawatek, Dawatek | 1 | 2020 | Leela Raavi, Davina Moossazadeh, Isaac Chadri 2020. Dawatek COVID protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bk24kygw | 2021-04-15 09:15:54 | ||||
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OnsiteGene 4 Protocol Saliva Extract Resource Report Resource Website |
yliu | DOI:10.17504/protocols.io.bpjemkje | OnsiteGene 1, XPRIZE Rapid Covid Testing | This OnsiteGene protocol is designed for testing the normal saliva samples with nucleic acid extraction. A normal saliva collection tube is used for the collection. The protocol uses the Star Array® Hi-SenseTM COVID-19 Molecular Testing Kit 1.0 in the one-step real-time RT-qPCR test assay to qualitatively detect RNA from SARS-CoV-2 virus in the human respiratory specimen. It combines the reverse transcription technology and real-time PCR method to provide accurate detection of the SARS-CoV-2 coronavirus. The Star Array® magnetic beads RNA extraction instrument and kit can automatically extract and purify the RNA for 32 samples in 6 minutes. It is ideal for the saliva samples that contain high amount of PCR inhibitors and can't be processed by the direct amplification. The triplex fluorescence design of the kit simultaneously detects the N1 and Orf1ab genes of the virus and the human RNase P gene as an internal control to ensure the sample quality. This protocol uses the Star Array® XDiveTM Superfast Real-Time PCR instrument to perform 40-cycle PCR in 8 minutes, and can test up to 16 samples or controls in each run. The total protocol time from sample collection to data interpretation is less than 15 minutes.The sample collection steps is adapted from the published protocol:https://www.protocols.io/view/saliva-collection-and-rna-extraction-for-sars-cov-bh6mj9c6 | OnsiteGene Inc. | 2 | 2020 | yliu 2020. OnsiteGene 4 Protocol Saliva Extract. protocols.io https://dx.doi.org/10.17504/protocols.io.bpjemkje | 2021-04-15 09:15:51 | |||
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Mmolecular COVID-19 Extraction-Free Direct-One-Step Fast Cycling Protocol Resource Report Resource Website |
steve.puts | DOI:10.17504/protocols.io.bkjpkumn | Coronavirus Method Development Community, Mmolecular, XPRIZE Rapid Covid Testing | The Direct One-Step RT-qPCR COVID-19 Test Kit is designed for quantitativereal-time analysis of target RNA directly from, swabs without the requirement of any priorRNA purification steps.Shipping: shipped on blue ice Storage Conditions: store at -20 °C Additional Storage Conditions: avoid freeze/thaw cycles stable at 4 °C for up to 4 weeks Shelf Life: 12 monthsForm: liquid / lyophilized | Mmolecular.com | 1 | 2020 | steve.puts 2020. Mmolecular COVID-19 Extraction-Free Direct-One-Step Fast Cycling Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bkjpkumn | 2021-04-15 09:15:58 | |||
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XPRIZE SHINE - In-tube Fluorescent SARS-CoV-2 NP Test Resource Report Resource Website |
Jon Arizti-Sanz, Catherine A. Freije, Chloe K. Boehm, Sameed M. Siddiqui, Allen M. Goodman, Tinna-Solveig F. Kosoko-Thoroddsen, A'Doriann Y. Bradley, Jeremy Johnson, Pardis C. Sabeti, Cameron Myhrvold | DOI:10.17504/protocols.io.bk4hkyt6 | XPRIZE Rapid Covid Testing | This protocol describes how to perform a SHINE in-tube fluorescent assay to detect SARS-CoV-2 RNA from a self-collected nasopharyngeal sample. This protocol is intended for point-of-care use. All enzymatic components are provided as a single-test freeze-dried pellet for shelf-stable storage, and all steps of the protocol are performed at ambient temperature. The protocol requires a transilluminator or another equivalent blue light emitting device. The protocol presented here is an improved version of the method presented in Arizti-Sanz J*, Freije CA*, et al. Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2. bioRxiv (2020). | Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Harvard-MIT Program in Health Sciences and Technology, 77 Massachusetts Ave., Cambridge, MA 02139, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Computational and Systems Biology PhD Program, MIT, Cambridge, MA 02139, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Harvard T.H. Chan School of Public Health, 677 Huntington Avenue, Boston, MA 02115, USA.; Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA.; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA., Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA. | 1 | 2020 | Jon Arizti-Sanz, Catherine A. Freije, Chloe K. Boehm, Sameed M. Siddiqui, Allen M. Goodman, Tinna-Solveig F. Kosoko-Thoroddsen, A'Doriann Y. Bradley, Jeremy Johnson, Pardis C. Sabeti, Cameron Myhrvold 2020. XPRIZE SHINE - In-tube Fluorescent SARS-CoV-2 NP Test. protocols.io https://dx.doi.org/10.17504/protocols.io.bk4hkyt6 | 2021-04-15 09:16:19 | |||
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mariPOC User manual.5.5.20 Resource Report Resource Website |
vincent.degennaro | API Pharma LLC, XPRIZE Rapid Covid Testing | ArcDia | 1 | 2020 | vincent.degennaro 2020. mariPOC User manual.5.5.20. protocols.io https:// | 2021-04-15 09:16:18 | |||||
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vBio Voice Analysis Resource Report Resource Website |
David Levesque | XPRIZE Rapid Covid Testing | vBio | 1 | 2020 | David Levesque 2020. vBio Voice Analysis. protocols.io https:// | 2021-04-15 09:16:17 | |||||
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ThEA Direct Swab Resource Report Resource Website |
Miriam Schwartz, Nikhil Ponon, Caleb Stewart, Luke Saban, Jody Ranck | DOI:10.17504/protocols.io.bkh4kt8w | Ram Global, XPRIZE Rapid Covid Testing | RAM Group has developed ThEA™ - an optical, reagent free / chemical free detection system for rapid diagnosis for SARS-Cov-2. The system consists of a THz spectrometer, a low-cost disposable sensor cartridge and a set of classification algorithms. ThEA’s The test includes taking an oropharyngeal swab from the patient and directly smearing it on the sensor surface followed by reading out the THz response in a THz time domain spectrometer. ThEA analysis software with classification algorithm will then be able to classify whether the sample is infectious with Sars-Cov-2 virus or not. | Ram Global, Ram Global, Ram Global, Ram Global, Ram Global | http://www.ramglobal.com | 1 | 2020 | Miriam Schwartz, Nikhil Ponon, Caleb Stewart, Luke Saban, Jody Ranck 2020. ThEA Direct Swab . protocols.io https://dx.doi.org/10.17504/protocols.io.bkh4kt8w | 2021-04-15 09:16:15 | ||
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microfluidic sars cov 2 lamp protocol Resource Report Resource Website |
Monica Simion, Alexandru Salceanu | DOI:10.17504/protocols.io.bkjjkukn | Coronavirus Method Development Community, covid19-test, XPRIZE Rapid Covid Testing | IMT, IMT | 1 | 2020 | Monica Simion, Alexandru Salceanu 2020. microfluidic sars cov 2 lamp protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bkjjkukn | 2021-04-15 09:16:18 | ||||
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Mask-Based Covid-10 testing system using Exhaled Breath Condensate Resource Report Resource Website |
John Daniels | DOI:10.17504/protocols.io.bkzzkx76 | Coronavirus Method Development Community, Front Line Technologies, XPRIZE Rapid Covid Testing, Team Exhaled Breath Condensate | The Kinaptic mask-based COVID-19 testing system uses a unique Exhaled Breath Condensate (EBC) collector that converts breath vapor to a liquid biosample for rapid testing and wireless automatic contact tracing. Exhaled Breath Condensate (EBC) is collected from a mask-based EBC collector as a low cost disposable component. Our electronic biosensor detects even low concentrations of virus biomarkers if they are present in the EBC sample. Corona viruses have slowly evolving N-protein and quickly evolving S-protein. N-Protein testing screens for any corona virus infection / S-Protein screens for only COVID-19 infection. The Kinaptic mask-based COVID-19 testing system is a “two-stage filter”: 1) catch only of N-protein biomarker = trigger warning to get more stringent diagnostic and contact trace; 2) catch both N- and S-, or just S- biomarkers = more urgent need for immediate quarantine and medical attention. As the target biomarkers bind to the capture molecules, the electrical conductivity of the electronic biosensor changes. This change in conductive is amplified by our bluetooth electronics, analyzed to determine the presence and quantity of the target biomarker, and the test result wireless transmitted to a smartphone or directly to the cloud. | Kinaptic, LLC | 1 | 2020 | John Daniels 2020. Mask-Based Covid-10 testing system using Exhaled Breath Condensate. protocols.io https://dx.doi.org/10.17504/protocols.io.bkzzkx76 | 2021-04-15 09:16:41 | |||
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LessTests Resource Report Resource Website |
Ruth Polachek, doreen | DOI:10.17504/protocols.io.bkiakuae | XPRIZE Rapid Covid Testing | LessTests SARS-CoV2 general diagnosis protocol, using LessTests software solution.The LessTests method is agnostic to the labware, and has already been demonstrated as successful using multiple device and kit combinations. | LessTests, LessTests | 1 | 2020 | Ruth Polachek, doreen 2020. LessTests. protocols.io https://dx.doi.org/10.17504/protocols.io.bkiakuae | 2021-04-15 09:16:22 | |||
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XPRIZE SANATA Protocol for Saliva LFIA Test Resource Report Resource Website |
Mario Thomas, Jasmine Sollen, Natalia Ivanova, Michelle Feng, Reda Fayek, Heidi Abdilla, Stephanie Lim, Amanda Naaum | DOI:10.17504/protocols.io.bqfrmtm6 | Coronavirus Method Development Community, XPRIZE Rapid Covid Testing, SANATA | This procedure outlines the protocol for testing for SARS-CoV-2 using a saliva sample collected from an individual. The purpose of this test is to detect low levels of SARS-CoV-2 antigen at a higher sensitivity. Precision Biomonitoring Inc. developed an ultra-rapid digital, disposable, highly-sensitive and inexpensive testing device used for screening purposes. The mobile app complementary to this medical device is connected through Bluetooth. Using this innovation, the user can be tested at point-of-care (POC) by a health care professional, and obtain qualitative results. | Precision Biomonitoring Inc., Precision Biomonitoring Inc., Precision Biomonitoring Inc., Precision Biomonitoring Inc., Precision Biomonitoring Inc., Precision Biomonitoring Inc., Precision Biomonitoring Inc., Precision Biomonitoring Inc. | 3 | 2020 | Mario Thomas, Jasmine Sollen, Natalia Ivanova, Michelle Feng, Reda Fayek, Heidi Abdilla, Stephanie Lim, Amanda Naaum 2020. XPRIZE SANATA Protocol for Saliva LFIA Test. protocols.io https://dx.doi.org/10.17504/protocols.io.bqfrmtm6 | 2021-04-15 09:16:22 | |||
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API Pharma Next Gen Antibody Test Resource Report Resource Website |
vincent.degennaro | DOI:10.17504/protocols.io.bjqqkmvw | Coronavirus Method Development Community, API Pharma LLC, XPRIZE Rapid Covid Testing | API Pharma LLC | 1 | 2020 | vincent.degennaro 2020. API Pharma Next Gen Antibody Test. protocols.io https://dx.doi.org/10.17504/protocols.io.bjqqkmvw | 2021-04-15 09:16:44 | ||||
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transmissive blood test Resource Report Resource Website |
, marcello.manfredi , Paolo Satta, Diogo Barros, Isabel Correa | DOI:10.17504/protocols.io.bj2pkqdn | XPRIZE Rapid Covid Testing | Tellspec LTD, Tellspec LTD, Tellspec LTD, Tellspec LTD, Tellspec LTD | 1 | 2020 | , marcello.manfredi , Paolo Satta, Diogo Barros, Isabel Correa 2020. transmissive blood test. protocols.io https://dx.doi.org/10.17504/protocols.io.bj2pkqdn | 2021-04-15 09:16:28 | ||||
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LooK SPOT Antigen Rapid Test System Resource Report Resource Website |
Diego Lai | DOI:10.17504/protocols.io.bp32mqqe | Coronavirus Method Development Community, XPRIZE Rapid Covid Testing | LooK SPOT COVID-19 antigen rapid test is a lateral flow immunoassay intended for the qualitative detection of nucleocapsid protein antigen from SARS-CoV-2 in nasal swabs from patients suspected of COVID-19 within the first seven (7) days of symptom onset. Testing is limited to laboratories certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA), 42 U.S.C. §263a, that meet the requirements to perform moderate complexity tests. This test is authorized for use at the Point of Care (POC), i.e., in a patient care settings operating under a CLIA Certificate of Waiver, Certificate of Compliance, or Certificate of Accreditation.].Results are for the identification of SARS-CoV-2 nucleocapsid protein antigen. The antigen is generally detectable in nasal samples during the acute phase of infection. Positive results indicate the presence of viral antigens, but the clinical correlation with patient history and other diagnostic information is necessary to determine infection status. Positive results do not rule out a bacterial infection or co-infection with other viruses. The agent detected may not be the definite cause of thedisease. Laboratories within the United States and its territories are required to report all positive results to the appropriate public health authorities.Negative results should be treated as presumptive, and do not rule out SARS-CoV-2 infection, and should not be used as the sole basis for treatment or patient management decisions, including infection control decisions. Negative results should be considered in the context of a patient's recent exposures, history, and the presence of clinical signs and symptoms consistent with COVID-19, and confirmed with a molecular assay, if necessary, for patient management. LooK SPOT COVID-19 Antigen Rapid Test can deliver a diagnosis of the SARS-CoV-2 virus detection between 5 to 8 minutes by using machine learning AI. LooK SPOT’s AI algorithm has high accuracy and can identify the fluorescence response when human eyes cannot identify the low positive cases. Healthcare responders in the COVID-19 test sites often need to make time-sensitive decisions to determine the test results during the time many patients are within their vicinity. But the tempo, volume, stress, fatigue, lighting, fear, and various other factors can overwhelm healthcare responders when making the visual interpretation of antigen test results. It is of paramount importance to reduce healthcare responders' cognitive load by providing accurate test results in an easy-to-read format. LooK SPOT COVID-19 Antigen Rapid Test is a COVID-19 rapid test solution designed with a tactical edge to fight COVID-19. The LooK SPOT COVID-19 Antigen Rapid Test is intended for use at the Point of Care (POC) settings by trained personnel specifically instructed and trained in vitro diagnostic procedures. It is only for use under the Food and Drug Administration's Emergency Use Authorization. | Laipac Technology Inc. | https://laipac.com/look-spot-point-of-care-covid-19-test-kit/ | 2 | 2020 | Diego Lai 2020. LooK SPOT Antigen Rapid Test System. protocols.io https://dx.doi.org/10.17504/protocols.io.bp32mqqe | 2021-04-15 09:16:56 |
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