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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: RANTES (Regulated on Activation, Normal T cell Expressed and Secreted) also known as CCL5 is a chemotactic cytokine for T cells, basophils, and eosinophils. RANTES also acts as a recruitment signal for immune cells into inflammatory sites.
Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of RANTES (Regulated on Activation, Normal T cell Expressed and Secreted) in human serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bj66krhe Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Protein AG (SpAG) is an immunoglobulin-binding protein that interacts with the Fc regions of many mammalian immunoglobulins [1].References1.Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175
Proper citation: Angel Justiz-Vaillant 2020. Direct ELISA for investigating the binding of recombinant or chemically-made Protein-AG to immunoglobulins.. protocols.io dx.doi.org/10.17504/protocols.io.bjxwkppe Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant 2020. Inhibition immunoassay of the HIVgp120_anti-HIVgp120 (Ab-1) reaction by anti-anti-idiotypic-HIVgp120 antibody (Ab-3). . protocols.io dx.doi.org/10.17504/protocols.io.bjnqkmdw Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Monocytes, T and B cells mostly make IL-24. It causes cancer-specific cell death, causes wound healing and protects against bacterial infections and cardiovascular diseases. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-24 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj3skqne Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. T cells produce IL-14, and its principal effects are stimulation of activated B cell proliferation and inhibition of immunoglobulin secretion. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-14 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj3dkqi6 Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Enzyme-linked immunosorbent assay (ELISA) for studying the presence of anti-HIV antibodies in layer hens, rats and cats was a reproducible and feseable test. The presence of the anti-HIV antibodies was further confirmed by dot blot analysis.
Proper citation: Angel Justiz-Vaillant 2020. Enzyme-linked immunosorbent assay (ELISA) for detection of anti-HIV antibodies (ELISA) developed in various animal species. . protocols.io dx.doi.org/10.17504/protocols.io.bjmzkk76 Copy
Authors: Sade Aisha Folashade John, Patrick E. Akpaka, Chandrashekhar Unakal, Arvind Kurhade, Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Enterobacteriaceae the leading cause of gastroenteritis. These gram- negative bacteria from species like Escherichia, Shigella, Salmonella, Vibrio and Helicobacter etc are among those that cause severe disease in consumers, especially those that indulge in uncooked seafood. Oysters have been shown to harbour these pathogenic organisms, which pose a major health risk. Numerous outbreaks of gastroenteritis due to oyster consumption have been reported worldwide because of the enteric bacteria as well as viral pathogen contaminationThis research was conducted to identify the Enterobacteriaceae present in oysters sold by vendors in Trinidad. During a five-month period (the rainy season, May - September), a total of 156 oyster samples (comprising 104 oysters samples and 52 water samples of the prepared, unprepared oyster cocktails plus the water used by vendors) were analysed. These samples were collected from roadside vendors operating near to the coastal line of the Western part of Trinidad These were processed at the microbiology unit of the Department of Para-clinical Sciences of the University of the West Indies, St. Augustine using standard microbiology techniques. The antibiotic susceptibility profiles of the recovered organisms were performed using the Kirby Bauer method for the following antibiotics- Tetracycline, Ampicillin, Trimethoprim-Sulfamethoxazole, Cefuroxime and Ceftazidime. The SPSS 21 programme was used to analyse the biometric data and a chi-square test was used to determine if there was any significant difference between the kinds of preparation cocktails with respect to the coliform and organisms found.Results of the 104 oyster samples tested, 112 isolates were obtained, 13.4% (15/112) were E.coli of which 0.1% (1/15) was the 0157:H7 strain; 72.3% (81/112) were Shigella species of which 27.2% (22/81) were Shigella dysenteriae; 14.3% (16/112) were Salmonella species of which 25% (4/16) were Salmonella cubana. The susceptibility profiles of the organisms revealed that 86.5% of Shigella, 100% of E.coli and 93.7% of the Salmonella were resistant to multiple antibiotics. With the recovery of these organisms from these samples, the health of the Trinidadian and foreign consumers of mangrove oysters is at risk particularly from Shigella, E.coli and Salmonella species. As a result, there should be an increased need for public awareness as well as regulations by the Ministry of Health to be made so that illness from these hazardous Enterobacteriaceae can be prevented.
Proper citation: Sade Aisha Folashade John, Patrick E. Akpaka, Chandrashekhar Unakal, Arvind Kurhade, Angel Justiz-Vaillant 2020. Detection of total and faecal coliforms in the oysters. protocols.io dx.doi.org/10.17504/protocols.io.bj6hkrb6 Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: A novel method was designed for the purification of Ab-2 from undiluted WSF of hyperimmune eggs. A total of six preparations were made.
Proper citation: Angel Justiz-Vaillant 2020. Purification of anti-anti-SpA antibody (Ab-2) using SpA-bearing Staphylococcus aureus cells.. protocols.io dx.doi.org/10.17504/protocols.io.bjj9kkr6 Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: This SpL ELISA can be used to detect specific antibodies in various animal species including human, mouse, rat, dog, rabbit, chicken, monkey, pig and hamster [1].1. De Chateau M, Nilson BH, Erntell M, Myhre E, Magnusson CG, Akerstrom B et al. On the interaction between protein L and immunoglobulins of various mammalian species. Scand J Immunol 1993; 37: 399−405
Proper citation: Angel Justiz-Vaillant 2020. Enzyme linked immunosorbent assay for investigating the binding of Streptococcal Protein-G (SpG) to diverse immunoglobulins. protocols.io dx.doi.org/10.17504/protocols.io.bjphkmj6 Copy
Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of CXC motif chemokine ligand 2 (CXCL2) in human serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bj7dkri6 Copy
Authors: Angel Justiz-Vaillant, Suzette Curtello
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant, Suzette Curtello 2020. Salmonella live attenuated vaccine preparation.. protocols.io dx.doi.org/10.17504/protocols.io.bjpdkmi6 Copy
Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of Granulocyte-colony stimulator factor (G-CSF) in human serum or plasma. protocols.io dx.doi.org/10.17504/protocols.io.bks2kwge Copy
Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Summary: Transforming growth factor beta (TGF-β) is a multifunctional cytokine belonging to the transforming growth factor superfamily. TGFB proteins are made by all white blood cell lineages.It is a multipotent growth factor affecting cell differentiation, tissue repair, cell proliferation, matrix production and apoptosis. [1]Reference1. Clark DA, Coker R. Transforming growth factor-beta (TGF-beta).Int J Biochem Cell Biol. 1998;30(3):293-298. doi:10.1016/s1357-2725(97)00128-3
Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of transforming growth factor beta (TGF-β) in human serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bj67krhn Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Monocytes produce IL-12. Its principal targets are T cells. It causes induction of Th1 cells. Besides, it is a potent inducer of interferon gamma production by T lymphocytes and NK cells. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-12 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj3bkqin Copy
Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human C4 in serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bj75krq6 Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. B lymphocytes mainly produce IL-39. It acts on neutrophils inducing their differentiation or expansion. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-39 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj4ekqte Copy
Authors: Angel Justiz-Vaillant, Norma McFarlane-Anderson
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Universal sandwich enzyme linked immunosorbent assay for investigating Staphylococcal protein-A (SpA) interactions with immunoglobulins using a SpLG-HRP conjugate.. protocols.io dx.doi.org/10.17504/protocols.io.bjpwkmpe Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant 2020. Determination of IgM concentration by the Mancini test.. protocols.io dx.doi.org/10.17504/protocols.io.bj9zkr76 Copy
Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for measurement of platelet-activating factor (PAF) in serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj7ikrke Copy
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Proper citation: Angel Justiz-Vaillant 2020. Universal Sandwich ELISA for investigating the binding of Protein-LAG (SpLAG) to avian immunoglobulins using anti-IgY-peroxidase as conjugate.. protocols.io dx.doi.org/10.17504/protocols.io.bjq5kmy6 Copy
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