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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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Dephosphorylation using AnP (M0289) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cdns5d | New England Biolabs (NEB) | This is the protocol for dephosphorylation of 5'-ends of DNA using AnP (Antarctic Phosphatase - M0289). | New England Biolabs | https://www.neb.com/protocols/1/01/01/vector-dephosphorylation-protocol | 1 | 2014 | New England Biolabs 2014. Dephosphorylation using AnP (M0289). protocols.io dx.doi.org/10.17504/protocols.io.cdns5d | 2021-03-29 03:12:31 | ||
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Enzymatic PCR Cleanup Protocol (NEB #M0525) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.7r4hm8w | New England Biolabs (NEB) | Rapid and irreversible heat inactivation eliminates unwanted activityImproved storage stability versus native enzymeFaster reaction setup (no supplemental additives like zinc required) and shorter incubation timeFlexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)Less enzyme required (high specific activity), resulting in a lower cost per reactionNo need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysisRecombinant for purity, consistency and value | New England Biolabs | https://neb.com/protocols/2019/06/04/enzymatic-pcr-cleanup-protocol-neb-m0525 | 1 | 2020 | New England Biolabs 2020. Enzymatic PCR Cleanup Protocol (NEB #M0525). protocols.io dx.doi.org/10.17504/protocols.io.7r4hm8w | 2021-03-29 03:12:35 | ||
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E2050 Modified Nucl Mixture Resource Report Resource Website |
NEB | 10.17504/protocols.io.cv8w9v | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2013/04/02/rna-synthesis-with-modified-nucleotides-e2050 | 1 | 2015 | NEB 2015. E2050 Modified Nucl Mixture. protocols.io dx.doi.org/10.17504/protocols.io.cv8w9v | 2021-03-29 03:12:06 | |||
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M0371 no RE mixture Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.crmv45 | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2013/06/10/protocol-for-dephosphorylation-of-5-ends-of-dna-m0371 | 1 | 2015 | New England Biolabs 2015. M0371 no RE mixture. protocols.io dx.doi.org/10.17504/protocols.io.crmv45 | 2021-03-29 03:12:13 | |||
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Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) E6310 (E7760) Resource Report Resource Website |
Lauren Moran, New England Biolabs | 10.17504/protocols.io.sxuefnw | New England Biolabs (NEB) | The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) depletes both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications. | New England Biolabs, New England Biolabs | 1 | 2018 | Lauren Moran, New England Biolabs 2018. Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) E6310 (E7760). protocols.io dx.doi.org/10.17504/protocols.io.sxuefnw | 2021-03-29 03:12:13 | |||
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Ultra II DNA Library Prep Kit for Illumina E7645/E7103 Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.mc5c2y6 | New England Biolabs (NEB) | The NEBNext Ultra II DNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of DNA into high quality libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow also has minimal hands-on time. | New England Biolabs | https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7645.pdf | 1 | 2018 | New England Biolabs 2018. Ultra II DNA Library Prep Kit for Illumina E7645/E7103. protocols.io dx.doi.org/10.17504/protocols.io.mc5c2y6 | 2021-03-29 03:10:04 | ||
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Cross-linking of IgG to Protein A or G Beads (S1425/S1430) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.crdv25 | New England Biolabs (NEB) | This protocol consists of an IgG purification step followed by covalent cross-linking of the IgG to the Protein A/G solid support. For IgG that has been previously purified, proceed directly to the cross-linking protocol. | New England Biolabs | https://www.neb.com/protocols/1/01/01/cross-linking-of-igg-to-protein-a-or-g-beads | 1 | 2015 | New England Biolabs 2015. Cross-linking of IgG to Protein A or G Beads (S1425/S1430). protocols.io dx.doi.org/10.17504/protocols.io.crdv25 | 2021-03-29 03:10:06 | ||
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Mixture for M0493 Q5 PCR Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cjsund | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2013/12/13/pcr-using-q5-high-fidelity-dna-polymerase-m0491 | 1 | 2015 | New England Biolabs 2015. Mixture for M0493 Q5 PCR. protocols.io dx.doi.org/10.17504/protocols.io.cjsund | 2021-03-29 03:10:13 | |||
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Sequential Double Digest Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cjmuk5 | New England Biolabs (NEB) | This is the Sequential Double Digest Protocol with Standard Restriction Enzymes. If there is no buffer in which the two enzymes exhibit > 50% activity, this sequential digest can be performed. | New England Biolabs | https://www.neb.com/protocols/2014/05/07/double-digest-protocol-with-standard-restriction-enzymes | 1 | 2014 | New England Biolabs 2014. Sequential Double Digest. protocols.io dx.doi.org/10.17504/protocols.io.cjmuk5 | 2021-03-29 03:10:13 | ||
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Dephosphorylation of 5´-ends of DNA using CIP (M0290) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.iqwcdxe | New England Biolabs (NEB) | Protocol for Dephosphorylation of 5´-ends of DNA using CIP in Restriction Enzyme Reaction. Uses the Calf Intestinal Alkaline Phosphatase (CIP - M0290) | New England Biolabs | https://www.neb.com/protocols/1/01/01/protocol-for-dephosphorylating-with-cip | 2 | 2017 | New England Biolabs 2017. Dephosphorylation of 5´-ends of DNA using CIP (M0290). protocols.io dx.doi.org/10.17504/protocols.io.iqwcdxe | 2021-03-29 03:10:18 | ||
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NEBuilder HiFi DNA Assembly Reaction (E2621) Resource Report Resource Website |
NEB | 10.17504/protocols.io.cwaxad | New England Biolabs (NEB) | This is the protocol for DNA Assembly using the NEBuilder® HiFi DNA Assembly Master Mix (E2621). | New England Biolabs | https://www.neb.com/protocols/2014/11/26/nebuilder-hifi-dna-assembly-reaction-protocol | 1 | 2015 | NEB 2015. NEBuilder HiFi DNA Assembly Reaction (E2621). protocols.io dx.doi.org/10.17504/protocols.io.cwaxad | 2021-03-29 03:11:55 | ||
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Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.nyfdftn | New England Biolabs (NEB) | Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030) | New England Biolabs | https://www.neb.com/protocols/2017/04/25/quick-protocol-for-oligonucleotide-cleanup-using-the-monarch-pcr-dna-cleanup-kit-5-g-neb-t1030 | 1 | 2018 | New England Biolabs 2018. Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030). protocols.io dx.doi.org/10.17504/protocols.io.nyfdftn | 2021-03-29 03:11:52 | ||
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Ligation Protocol for NEB PCR Cloning Kit (E1202) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cp5vq5 | New England Biolabs (NEB) | Ligation protocol for the PCR Cloning Kit (E1202) | New England Biolabs | https://www.neb.com/protocols/2013/12/27/ligation-protocol-e1202 | 1 | 2015 | New England Biolabs 2015. Ligation Protocol for NEB PCR Cloning Kit (E1202). protocols.io dx.doi.org/10.17504/protocols.io.cp5vq5 | 2021-03-29 03:11:55 | ||
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Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.bp9fmr3n | New England Biolabs (NEB) | This is the quick version of the Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010). For the full protocol, please click here. | New England Biolabs | https://www.neb.com/protocols/2015/12/08/quick-protocol-for-monarch-plasmid-miniprep-kit-t1010 | 3 | 2020 | New England Biolabs 2020. Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010). protocols.io dx.doi.org/10.17504/protocols.io.bp9fmr3n | 2021-03-29 03:11:57 | ||
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NEBNext Ultra End Prep Mixture E7442 Resource Report Resource Website |
Isabel Gautreau | 10.17504/protocols.io.k6yczfw | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2012/11/15/nebnext-end-prep-e7370 | 3 | 2020 | Isabel Gautreau 2020. NEBNext Ultra End Prep Mixture E7442. protocols.io dx.doi.org/10.17504/protocols.io.k6yczfw | 2021-03-29 03:11:57 | |||
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High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019H) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.jxycppw | New England Biolabs (NEB) | This is the correct protocol if you are using the C3019H cells. If you are using the C3019I cells, please refer to this protocol. | NEB, 240 County RoadIpswich, MA 01938-2723 | https://www.neb.com/protocols/1/01/01/high-efficiency-transformation-protocol-c3019 | 3 | 2017 | New England Biolabs 2017. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019H). protocols.io dx.doi.org/10.17504/protocols.io.jxycppw | 2021-03-29 03:11:58 | ||
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OneTaq Mixture M0480 Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cq9vz5 | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2012/10/11/onetaqdnapolymerasem0480 | 1 | 2015 | New England Biolabs 2015. OneTaq Mixture M0480. protocols.io dx.doi.org/10.17504/protocols.io.cq9vz5 | 2021-03-29 03:11:59 | |||
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High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019I) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cf5tq5 | New England Biolabs (NEB) | This is the correct protocol if you are using the C3019I cells. If you are using the C3019H cells, please refer to this protocol. | New England Biolabs | https://www.neb.com/protocols/1/01/01/high-efficiency-transformation-protocol-c3019 | 1 | 2015 | New England Biolabs 2015. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019I). protocols.io dx.doi.org/10.17504/protocols.io.cf5tq5 | 2021-03-29 03:12:01 | ||
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Digestion with NEBNext dsDNA Fragmentase (M0348) Resource Report Resource Website |
Isabel Gautreau | 10.17504/protocols.io.e9rbh56 | New England Biolabs (NEB) | Protocol for digestion with NEBNext dsDNA Fragmentase (M0348) | New England Biolabs | https://www.neb.com/protocols/1/01/01/digestion-with-nebnext-dsdna-fragmentase-m0348 | 2 | 2016 | Isabel Gautreau 2016. Digestion with NEBNext dsDNA Fragmentase (M0348). protocols.io dx.doi.org/10.17504/protocols.io.e9rbh56 | 2021-03-29 03:11:59 | ||
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NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®) E7658 Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.bsy5nfy6 | New England Biolabs (NEB), Coronavirus Method Development Community | This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®), NEB #E7658S/L 24/96 reactions. | New England Biolabs | https://www.neb.com/-/media/nebus/files/manuals/manuale7658.pdf?rev=7253d569aa3040069cb09a9aa3724f2d&hash=53C7EDF0D2FDEDAE75308379A5EB2D89 | 2 | 2021 | New England Biolabs 2021. NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®) E7658. protocols.io dx.doi.org/10.17504/protocols.io.bsy5nfy6 | 2021-03-29 03:12:02 |
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