Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Precision Count Beads™ Protocol and Applications Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.baariad6 | BioLegend | Precision Count Beads™ are designed for counting the absolute number of cells in a complex mix population and other particles by flow cytometry. Precision Count Beads™ are excited by a variety of lasers including violet (405nm), blue (488nm), yellow/green (562nm), and red (633nm). | BioLegend | https://www.biolegend.com/protocols/precision-count-beads-protocol-and-applications/4248/ | 2 | 2019 | Sam Li 2019. Precision Count Beads™ Protocol and Applications. protocols.io https://dx.doi.org/10.17504/protocols.io.baariad6 | 2021-04-15 09:16:06 | ||
|
Immunocytochemistry Staining Protocol Resource Report Resource Website |
BioLegend, Inc., Kelsey Knight | DOI:10.17504/protocols.io.tdmei46 | BioLegend | , , BioLegend | http://www.biolegend.com/media_assets/support_protocol/Immunofluorescence_Microscopy_Protocol_050514.pdf | 3 | 2018 | BioLegend, Inc., Kelsey Knight 2018. Immunocytochemistry Staining Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.tdmei46 | 2021-04-15 09:16:03 | |||
|
MojoSort™ Mouse CD4 Nanobeads Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.696hh9e | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-cd4-nanobeads-column-protocol/4766/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse CD4 Nanobeads Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.696hh9e | 2021-04-15 09:16:07 | ||
|
MojoSort™ Selection Kits Protocol - 5 Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7z9hp96 | BioLegend | Product description and procedure summary:Target cells are either selected or depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads (Cat. No. 480015/480016). The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. If these are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.Sample Preparation:Enzymatic digestion of mouse brain followed by myelin removal is recommended to achieve the highest purity and yield. There are several protocols published that can be applied. As a general guideline, Trypsin digestion followed by a 70/37/30% percoll gradient will increase final purity and yield.Scale up volumes if using 14 ml tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-selection-kits-protocol-5/4244/ | 1 | 2019 | Sam Li 2019. MojoSort™ Selection Kits Protocol - 5. protocols.io https://dx.doi.org/10.17504/protocols.io.7z9hp96 | 2021-04-15 09:16:08 | ||
|
Whole Mouse Brain Processing for Microglia Isolation, Cell Separation, and Flow Cytometry Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.97dh9i6 | BioLegend | BioLegend | https://www.biolegend.com/en-us/protocols/whole-mouse-brain-processing-for-microglia-isolation-cell-separation-and-flow-cytometry | 1 | 2019 | Sam Li 2019. Whole Mouse Brain Processing for Microglia Isolation, Cell Separation, and Flow Cytometry. protocols.io https://dx.doi.org/10.17504/protocols.io.97dh9i6 | 2021-04-15 09:16:09 | |||
|
Intracellular Staining With True-Phos™ Perm Buffer in Whole Blood Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e2dbga6 | BioLegend | BioLegend | http://www.biolegend.com/media_assets/support_protocol/Intracellular_Staining_with_TruePhos_Perm_Buffer_in_Whole_Blood_05052016v2_DMS_Update.pdf | 1 | 2016 | Kelsey Miller 2016. Intracellular Staining With True-Phos™ Perm Buffer in Whole Blood. protocols.io https://dx.doi.org/10.17504/protocols.io.e2dbga6 | 2021-04-15 09:16:09 | |||
|
MojoSort™ Human CD56 Nanobeads Protocol Resource Report Resource Website |
Ken Lau | BioLegend | BioLegend | https://www.biolegend.com/en-us/protocols/mojosort-human-cd56-nanobeads-protocol | 2 | 2021 | Ken Lau 2021. MojoSort™ Human CD56 Nanobeads Protocol. protocols.io https:// | 2021-04-15 09:16:12 | ||||
|
MojoSort™ Mouse CD11c Nanobeads Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7abhian | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-cd11c-nanobeads-column-protocol/4768/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse CD11c Nanobeads Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7abhian | 2021-04-15 09:16:15 | ||
|
Ethanol Fixation Protocol Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e24bggw | BioLegend | Cell Fixation and Permeabilization Protocol Using 70% Ethanol | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BioLegend_EthFIXPERM_032912.pdf | 1 | 2016 | Kelsey Miller 2016. Ethanol Fixation Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.e24bggw | 2021-04-15 09:16:15 | ||
|
Treg Polarization of Mouse CD4+ Cells Resource Report Resource Website |
BioLegend, Inc. | DOI:10.17504/protocols.io.ex7bfrn | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/BioLegend_Treg_Polarization_of_Mouse_CD4_Cells_012715.pdf | 1 | 2016 | BioLegend, Inc. 2016. Treg Polarization of Mouse CD4+ Cells. protocols.io https://dx.doi.org/10.17504/protocols.io.ex7bfrn | 2021-04-15 09:16:20 | |||
|
Intracellular Staining With True-Phos™ Perm Buffer in Cell Suspensions Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bac4iayw | BioLegend | BioLegend | https://www.biolegend.com/protocols/intracellular-staining-with-true-phos-perm-buffer-in-cell-suspensions-protocol/4262/ | 2 | 2019 | Sam Li 2019. Intracellular Staining With True-Phos™ Perm Buffer in Cell Suspensions. protocols.io https://dx.doi.org/10.17504/protocols.io.bac4iayw | 2021-04-15 09:16:33 | |||
|
MojoSort™ Human CD45 Nanobeads Protocol 2 - CD45 greater than 50% Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7wqhpdw | BioLegend | Product description and procedure summary:If the percentage of CD45+ cells in your sample is less than 50%, please follow Human CD45 Nanobeads Protocol 1. If it is higher than 50% then please follow Human CD45 Nanobeads Protocol 2. The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-cd45-nanobeads-protocol-2-cd45-greater-than-50/4277/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human CD45 Nanobeads Protocol 2 - CD45 greater than 50%. protocols.io https://dx.doi.org/10.17504/protocols.io.7wqhpdw | 2021-04-15 09:16:18 | ||
|
Th17 Polarization of Mouse CD4+ Cells Resource Report Resource Website |
BioLegend, Inc. | DOI:10.17504/protocols.io.ezrbf56 | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/BioLegend_Th17_Polarization_of_Mouse_CD4_Cells_012715.pdf | 1 | 2016 | BioLegend, Inc. 2016. Th17 Polarization of Mouse CD4+ Cells. protocols.io https://dx.doi.org/10.17504/protocols.io.ezrbf56 | 2021-04-15 09:16:18 | |||
|
Precision Count Beads™ Protocol and Applications Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e3cbgiw | BioLegend | Precision Count Beads™ are designed for counting the absolute number of cells in a complex mix population and other particles by flow cytometry. Precision Count Beads™ are excited by a variety of lasers including violet (405nm), blue (488nm), yellow/green (562nm), and red (633nm). | BioLegend | http://www.biolegend.com/media_assets/support_protocol/Precision_Count_Beads_Protocol_04262016.pdf | 1 | 2016 | Kelsey Miller 2016. Precision Count Beads™ Protocol and Applications. protocols.io https://dx.doi.org/10.17504/protocols.io.e3cbgiw | 2021-04-15 09:16:21 | ||
|
Ki-67 Staining Protocol Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.fabbian | BioLegend | BioLegend | http://www.biolegend.com/media_assets/support_protocol/Ki67_Staining_Protocol.pdf | 1 | 2016 | Kelsey Miller 2016. Ki-67 Staining Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.fabbian | 2021-04-15 09:16:38 | |||
|
Mojosort™ Mouse Neutrophil Isolation Kit Protocol Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.fkabkse | BioLegend | Non Neutrophil cells are depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Mouse_Neutrophil_Isolation_Protocol_08072016.pdf | 1 | 2016 | Kelsey Miller 2016. Mojosort™ Mouse Neutrophil Isolation Kit Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.fkabkse | 2021-04-15 09:16:23 | ||
|
Human T Cell Activation with anti-CD3 Anitbodies (clone UCHT1, OKT3 or HIT3a) Resource Report Resource Website |
BioLegend, Inc. | DOI:10.17504/protocols.io.extbfnn | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/BioLegend_T_cell_Activation_Protocol_012715.pdf | 1 | 2016 | BioLegend, Inc. 2016. Human T Cell Activation with anti-CD3 Anitbodies (clone UCHT1, OKT3 or HIT3a). protocols.io https://dx.doi.org/10.17504/protocols.io.extbfnn | 2021-04-15 09:16:25 | |||
|
MojoSort™ Mouse Neutrophil Isolation Kit Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7bjhikn | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-neutrophil-isolation-kit-column-protocol/4770/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse Neutrophil Isolation Kit Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7bjhikn | 2021-04-15 09:16:25 | ||
|
Anti-BrdU Staining Protocols Using DNAse with Surface and Fluorescent Proteins Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bac9iaz6 | BioLegend | BioLegend | https://www.biolegend.com/protocols/anti-brdu-staining-using-dnase-with-surface-and-fluorescent-proteins/4241/ | 3 | 2019 | Sam Li 2019. Anti-BrdU Staining Protocols Using DNAse with Surface and Fluorescent Proteins. protocols.io https://dx.doi.org/10.17504/protocols.io.bac9iaz6 | 2021-04-15 09:16:25 | |||
|
True-Nuclear™ Transcription Factor Staining Protocol for 96-Well, U-Bottom Plate Resource Report Resource Website |
BioLegend, Inc. | DOI:10.17504/protocols.io.gq5bvy6 | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/True-Nuclear_Trademark_Transcription_Factor_Staining_Protocol.pdf | 2 | 2016 | BioLegend, Inc. 2016. True-Nuclear™ Transcription Factor Staining Protocol for 96-Well, U-Bottom Plate. protocols.io https://dx.doi.org/10.17504/protocols.io.gq5bvy6 | 2021-04-15 09:16:23 |
Can't find your Protocol?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific protocol and you know the DOI of the protocol already, it's easier to enter a DOI to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your protocol in the search results, please help us by adding it into the system — it's easy. Create and publish your protocols at Protocols.io.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
