Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the PCR protocol for Phusion® High-Fidelity DNA Polymerase (M0530)
Proper citation: New England Biolabs 2015. PCR with Phusion® Polymerase (M0530). protocols.io https://dx.doi.org/10.17504/protocols.io.cdjs4m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for the Q5® Site-Directed Mutagenesis Kit (E0554)
Proper citation: New England Biolabs 2014. Q5® Site-Directed Mutagenesis (E0554). protocols.io https://dx.doi.org/10.17504/protocols.io.cjpumm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. OneTaq Mixture M0480. protocols.io https://dx.doi.org/10.17504/protocols.io.cq9vz5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. NEB Immunoprecip. Buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.crbv2m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) depletes both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications.
Proper citation: New England Biolabs 2018. NEBNext rRNA Depletion Kit (Human/Mouse/Rat) E6310. protocols.io https://dx.doi.org/10.17504/protocols.io.mzkc74w Copy
Authors: Lauren Moran, New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) depletes both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications.
Proper citation: Lauren Moran, New England Biolabs 2018. Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) E6310 (E7760). protocols.io https://dx.doi.org/10.17504/protocols.io.sxuefnw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. T4 PNK Radioactive Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.cp8vrv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This transformation protocol is to be performed directly in the C2527H tubes. (For the C2527I protocol, see here.)
Proper citation: New England Biolabs 2015. Transformation Protocol for BL21(DE3) Competent Cells (C2527H). protocols.io https://dx.doi.org/10.17504/protocols.io.criv4d Copy
Authors: New England Biolabs, Menna Teffera
Group: New England Biolabs (NEB)
Summary: The NEBNext dA-Tailing Module has been optimized to efficiently incorporate a non-templated dAMP on the 3 ́ end of a blunt DNA fragment (1). 3 ́-dA DNA tailing prevents concadamer formation during subsequent ligation steps.
DNA tailed with the NEBNext dA-Tailing module may be ligated to adaptors or cloning vectors with complementary dT overhangs. The NEBNext dA-Tailing Module is provided as a master mix to maximize efficiency and convenience in DNA sample preparation workflows.
The NEBNext dA-Tailing Module has been validated by sequencing with the Il- lumina Genome Analyzer II (Illumina, Inc.) in conjunction with the NEBNext End Repair Module, NEBNext Quick Ligation Module and Phusion® High-Fidelity PCR Master Mix.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
Proper citation: New England Biolabs, Menna Teffera 2019. NEBNext dA-Tailing Module (NEB #E6053). protocols.io https://dx.doi.org/10.17504/protocols.io.4t3gwqn Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for the removal of single-stranded extension using Mung Bean Nuclease (M0250).
Proper citation: New England Biolabs 2015. Removal of Single-Stranded Extension using Mung Bean Nuclease (M0250). protocols.io https://dx.doi.org/10.17504/protocols.io.crhv35 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2020. Purification of RNA from the Aqueous Phase Following TRIzol®/Chloroform Extraction using the Monarch® RNA Cleanup Kits. protocols.io https:// Copy
Authors: New England Biolabs
Proper citation: New England Biolabs 2014. Restriction Digest Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.chjt4m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Protocol for non-radioactive phosphorylation with T4 PNK.
Proper citation: New England Biolabs 2015. Non-radioactive phosphorylation with T4 PNK. protocols.io https://dx.doi.org/10.17504/protocols.io.cpdvi5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocol is for PCR with Q5® High-Fidelity 2X Master Mix (M0492)
Proper citation: New England Biolabs 2014. PCR with Q5® High-Fidelity 2X Master Mix (M0492). protocols.io https://dx.doi.org/10.17504/protocols.io.ciiucd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. BioBrick E0546 Downstream Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.cpkvkv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the Double Digest Protocol with Standard Restriction Enzymes, in the same buffer, using two separate incubation temperatures for the enzymes.
Proper citation: New England Biolabs 2014. Multi-temperature Double Digest (single buffer). protocols.io https://dx.doi.org/10.17504/protocols.io.cjkukv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2020. Quick Protocol for Extraction and Purification of Genomic DNA. protocols.io https:// Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA. This protocol describes how to determine genome targeting efficiency by digesting annealed PCR products with T7 Endonuclease I. In the first step PCR products are produced from the genomic DNA of cells whose genomes were targeted using Cas9, TALEN, ZFN etc. In the second step, the PCR products are annealed and digested with T7 Endonuclease I. Fragments are analyzed to determine the efficiency of genome targeting.
Proper citation: New England Biolabs 2016. Determining Genome Targeting Efficiency using T7 Endonuclease I (M0302). protocols.io https://dx.doi.org/10.17504/protocols.io.etbbein Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Radioactive Labeling with T4 Polynucleotide Kinase 3' phosphatase minus
Proper citation: New England Biolabs 2015. Radioactive Labeling with T4 PNK 3' phosphatase minus. protocols.io https://dx.doi.org/10.17504/protocols.io.cp9vr5 Copy
Authors: New England Biolabs
Group: Innovative Genomics Institute, CornLab
Proper citation: New England Biolabs 2015. SOC Media. protocols.io https://dx.doi.org/10.17504/protocols.io.crsv6d Copy
Can't find your Protocol?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific protocol and you know the DOI of the protocol already, it's easier to enter a DOI to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your protocol in the search results, please help us by adding it into the system — it's easy. Create and publish your protocols at Protocols.io.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
