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Authors: Angel Justiz-Vaillant
Group: Carbon
Summary: This ELISA was used to study the interactions between Staphylococcal protein-G (SpG) and protein-AG (SpAG) with different immunoglobulin preparations from mammalian and avian species.

Proper citation: Angel Justiz-Vaillant 2021. Streptococcal protein G and Protein-AG sandwich ELISA. protocols.io https://dx.doi.org/10.17504/protocols.io.bta2nige Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Keyhole limpet hemocyanin (KLH) is a cooper-containing protein comprising of subunits with MW of 400 kDa. This protein is found in the hemolymph of the sea mollusk Megathura crenulata. It has the ability to enhance the host’s immune response by interacting with monocytes, T cells and macrophages. KLH has been used primarily as a carrier for vaccines and antigens [1]. It was found that chicken immunized with KLH bound peptide raised an anti-KLH immunoresponse [2]. This can be tested by a single method such as the Ouchterlony technique.Reference1. Aarntzen EH, de Vries IJ, Göertz JH, et al. Humoral anti-KLH responses in cancer patients treated with dendritic cell-based immunotherapy are dictated by different vaccination parameters.Cancer Immunol Immunother. 2012;61(11):2003-2011. doi:10.1007/s00262-012-1263-z2. Justiz Vaillant AA, Anderson MF, Smikle M, Wisdom B, Mohammed W, et al. (2013) Development of Anti HIV Gp120 and HIV Gp41 Peptide Vaccines. J Vaccines Vaccin 4: 206. doi: 10.4172/2157-7560.1000206

Proper citation: Angel Justiz-Vaillant 2020. Detection of anti- SpA antibodies in egg white tested by double immunodiffusion (Ouchterlony) technique.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjsqkndw Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant 2020. Immunoblot analyses for investigating SpLA binding to purified mammalian and avian immunoglobulins.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjq7kmzn Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. It is strongly associated inflammatory activity with IL-26. Th17 cells produce this interleukin. It acts on epithelial cells and intestinal epithelial cells. It induces IL-10 expression, stimulates the production of IL-1-beta, IL-6, and IL-8 and causes Th17 cell generation. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-26 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj3vkqn6 Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Enzyme-Linked Immunosorbent Assay (ELISA) was used for screening while the Western Blot was used for confirmation.

Proper citation: Angel Justiz-Vaillant 2020. Immunoblot analysis for immunodetection of HIV proteins.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjtpknmn Copy   

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Authors: Angel Justiz-Vaillant
Group: Carbon
Summary: This ELISA was used to study the interactions between SpG) and SpLG) with different immunoglobulin preparations from mammalian and avian species.

Proper citation: Angel Justiz-Vaillant 2021. Streptococcal Protein-G and Protein-LG sandwich ELISA. protocols.io https://dx.doi.org/10.17504/protocols.io.btaznif6 Copy   

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Authors: Angel Justiz-Vaillant, Norma McFarlane-Anderson
Group: University of the West Indies, [email protected]
Summary: This protocol was already used successfully to detect anti-HIV antibody in the serum of women with cervical dysplasia or cervical cancer in Jamaica, West Indies [1].Reference1. Justiz Vaillant A, Bazuaye PE, McFarlane-Anderson N, Smikle MF et al. Seroprevalence of Anti-HIV Antibodies in Women with Abnormal Pap Smears in Jamaica. British Journal of Medicine & Medical Research 2013, 3(4): 2197-2202.

Proper citation: Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Indirect Enzyme Linked Immunosorbent Assay (ELISA) for Detection of Anti-HIV Antibodies in Human Serum. protocols.io https://dx.doi.org/10.17504/protocols.io.bkbmksk6 Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. They have both paracrine and autocrine function. Interleukins are also used in animal studies to investigate aspect related to clinical medicine.[1]Macrophages, large granular lymphocytes, B cells, endothelium, fibroblasts, and astrocytes secrete IL-1. T cells, B cells, macrophages, endothelium and tissue cells are the principal targets. IL-1 causes lymphocyte activation, macrophage stimulation, increased leukocyte/endothelial adhesion, fever due to hypothalamus stimulation, and release of acute phase proteins by the liver. It may also cause apoptosis in many cell types and cachexia.[2]Reference1. Zhu Z, Wang D, Jiao W, Chen G, Cao Y, Zhang Q, Wang J. Bioinformatics analyses of pathways and gene predictions in IL-1α and IL-1β knockout mice with spinal cord injury. Acta Histochem. 2017 Sep;119(7):663-670. [PubMed]2. Boraschi D, Bossu P, Macchia G, Ruggiero P, Tagliabue A. Structure-function relationship in the IL-1 family. Front. Biosci. 1996 Oct 01;1:d270-308. [PubMed]

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-1 in human serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bkerktd6 Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: This immunoassay tested the ability of anti-anti-SpA (Ab-2) to inhibit or interfere with the binding of anti-anti-anti-SpA (Ab-3) to the original antigen- staphylococcal protein A (SpA). It is a confirmation test of the functional capacity of Ab-2. In this ELISA, both Ab-2 and the antigen SpA compete for binding to Ab-3. When Ab-2 is present, it blocks the binding of HRP-labeled SpA to Ab-3, resulting in inhibition of reaction color development.

Proper citation: Angel Justiz-Vaillant 2020. Competitive ELISA to study the inhibition of the SpA- binding to Ab-3 by Ab-2.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjkbkksn Copy   

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Authors: Angel Justiz-Vaillant, Norma McFarlane-Anderson
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Universal sandwich enzyme linked immunosorbent assay for investigating protein-LG (SpLG) interactions with immunoglobulins using a SpA-HRP conjugate.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjp4kmqw Copy   

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Authors: Angel Justiz-Vaillant, Norma McFarlane-Anderson
Group: University of the West Indies, [email protected]
Summary: This SpLAG ELISA can be used to detect specific antibodies in various animal species including human, goat, donkey, mouse, rat, dog, cow, horse, ostrich, bantam hen, rabbit, chicken, monkey, pig, hamster, and many wild and zoo animals species [1].1. Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175

Proper citation: Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Enzyme linked immunosorbent assay for investigating the binding of chemically prepared protein-LAG (SpLAG) to immunoglobulins.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjprkmm6 Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant 2020. Enzyme linked immunosorbent assay (ELISA) for determining the serum concentration of IL-33 in humans.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjy9kpz6 Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. CD4+T cells (Th2), NKT cells and mast cells synthesize IL-13. It acts on monocytes, fibroblasts, epithelial cells and B cells. The IL-13 significant effects are B-cell growth and differentiation, stimulates isotype switching to IgE. It causes increased mucus production by epithelial cells, increased collagen synthesis by fibroblasts and inhibits pro-inflammatory cytokine production. Also, IL-13 works together with IL-4 in producing biologic effects associated with allergic inflammation and in defense against parasites. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-13 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj3ckqiw Copy   

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Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Summary: The monocyte chemoattractant protein-1(MCP-1/CCL2) is a member of the C-C chemokine family, and it is a potent chemotactic factor for monocytes.

Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of monocyte chemoattractant protein-1 (MCP-1/CCL2) in human serum or plasma. protocols.io https://dx.doi.org/10.17504/protocols.io.bktmkwk6 Copy   

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Authors: Angel Justiz-Vaillant
Group: Carbon
Summary: This ELISA was used to study the interactions between Staphylococcal protein-L (SpL) and protein-LG with different immunoglobulin preparations from mammalian and avian species.

Proper citation: Angel Justiz-Vaillant 2021. Peptostreptococcal Protein L and Protein LG sandwich ELISA. protocols.io https://dx.doi.org/10.17504/protocols.io.bta4nigw Copy   

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Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human C2 in serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj73krqn Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. IL-31 is produced mainly by Th2 cells and dendritic cells. It is a proinflammatory cytokine and a chemotactic factor that direct polymorphonuclear cells, monocytes, and T cells to inflammatory lesions. IL-31 induces chemokines production and synthesis of IL-6, IL-16, and IL-32. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.

Proper citation: Angel Justiz-Vaillant 2020. ELISA for quantification of IL-31 in human serum.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj34kqqw Copy   

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Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Summary: Transforming growth factor beta (TGF-β) is a multifunctional cytokine belonging to the transforming growth factor superfamily. TGFB proteins are made by all white blood cell lineages.It is a multipotent growth factor affecting cell differentiation, tissue repair, cell proliferation, matrix production and apoptosis. [1]Reference1. Clark DA, Coker R. Transforming growth factor-beta (TGF-beta).Int J Biochem Cell Biol. 1998;30(3):293-298. doi:10.1016/s1357-2725(97)00128-3

Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of transforming growth factor beta (TGF-β) in human serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj67krhn Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: Keyhole limpet hemocyanin (KLH) is a cooper-containing protein comprising of subunits with MW of 400 kDa. This protein is found in the hemolymph of the sea mollusk Megathura crenulata. It has the ability to enhance the host’s immune response by interacting with monocytes, T cells and macrophages. KLH has been used primarily as a carrier for vaccines and antigens [1]. It was found that chicken immunized with KLH bound peptide raised an anti-KLH immunoresponse [2]. This can be tested by a single method such as the Ouchterlony technique.Reference1. Aarntzen EH, de Vries IJ, Göertz JH, et al. Humoral anti-KLH responses in cancer patients treated with dendritic cell-based immunotherapy are dictated by different vaccination parameters.Cancer Immunol Immunother. 2012;61(11):2003-2011. doi:10.1007/s00262-012-1263-z2. Justiz Vaillant AA, Anderson MF, Smikle M, Wisdom B, Mohammed W, et al. (2013) Development of Anti HIV Gp120 and HIV Gp41 Peptide Vaccines. J Vaccines Vaccin 4: 206. doi: 10.4172/2157-7560.1000206

Proper citation: Angel Justiz-Vaillant 2020. Detection of anti- keyhole limpet hemocynin (anti-KLH) antibodies by double immunodiffusion (Ouchterlony) technique.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjspkndn Copy   

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Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]

Proper citation: Angel Justiz-Vaillant 2020. Determination of C4 concentration by the Mancini test.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj95kr86 Copy   

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