Protocols   Select Another Resource Report Type

Authors: Hsiao-Chung Tsai
Group: Coronavirus Method Development Community, ProtectLife-AnTaimmu, XPRIZE Rapid Covid Testing

Proper citation: Hsiao-Chung Tsai 2020. ENNOLIFE SARS-CoV-2 Antigen Test Kit Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bke3ktgn Copy   

•   Source:

Authors: beldenwj
Group: XPRIZE Rapid Covid Testing
Summary: This is a simple slot/dot blot protocol for detecting antigen on a membrane. Using the method outline here, one can achieve results in under 90 min because it eliminates the need for running a gel, transfering the protein in a transfer apparatus, blocking, secondary antibody incubation and the washes between primary and secondary antibodies.

Proper citation: beldenwj 2020. Slot/Dot Blot protocol.. protocols.io https://dx.doi.org/10.17504/protocols.io.bk3mkyk6 Copy   

•   Source:

Authors: Rob Parks, Barton Haynes
Group: XPRIZE Rapid Covid Testing

Proper citation: Rob Parks, Barton Haynes 2020. SARS-CoV-2 Antigen Detection ELISA. protocols.io https://dx.doi.org/10.17504/protocols.io.bk2ckyaw Copy   

•   Source:

Authors: Jon Arizti-Sanz, Catherine A. Freije, Chloe K. Boehm, Sameed M. Siddiqui, Allen M. Goodman, Tinna-Solveig F. Kosoko-Thoroddsen, A'Doriann Y. Bradley, Jeremy Johnson, Pardis C. Sabeti, Cameron Myhrvold
Group: XPRIZE Rapid Covid Testing
Summary: This protocol describes how to perform a SHINE paper-based assay to detect SARS-CoV-2 RNA from a self-collected saliva sample. This protocol is intended for in-home use. All enzymatic components are provided as a single-test freeze-dried pellet for shelf-stable storage, and all steps of the protocol are performed at ambient temperature. The protocol presented here is an improved version of the method presented in Arizti-Sanz J*, Freije CA*, et al. Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2. bioRxiv (2020).

Proper citation: Jon Arizti-Sanz, Catherine A. Freije, Chloe K. Boehm, Sameed M. Siddiqui, Allen M. Goodman, Tinna-Solveig F. Kosoko-Thoroddsen, A'Doriann Y. Bradley, Jeremy Johnson, Pardis C. Sabeti, Cameron Myhrvold 2020. XPRIZE SHINE - Paper-based SARS-CoV-2 Saliva Test. protocols.io https://dx.doi.org/10.17504/protocols.io.bk2fkybn Copy   

•   Source:

Authors:
Group: Narwhale for XPrize, XPRIZE Rapid Covid Testing
Summary: GM Covid-19 saliva test is a RT-PCR test intended for qualitative detection of nucleic acids from SARS-CoV-2 in saliva specimen. The test is used for screening purpose under class of "research used only" (RUO). The test is not for diagnostic purpose. Positive result would be referred to an official clinical test with appropriate certification (i.e CLIA) required for clinical diagnosis.

Proper citation: 2020. GM Covid-19 saliva test (v1). protocols.io https://dx.doi.org/10.17504/protocols.io.bkgfkttn Copy   

•   Source:

Authors: philippe.bechtold
Group: XPRIZE Rapid Covid Testing

Proper citation: philippe.bechtold 2020. Sample preparation. protocols.io https://dx.doi.org/10.17504/protocols.io.bjygkptw Copy   

•   Source:

Authors: Leela Raavi, Davina Moossazadeh, Isaac Chadri
Group: XPRIZE Rapid Covid Testing

Proper citation: Leela Raavi, Davina Moossazadeh, Isaac Chadri 2020. Dawatek COVID protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bk24kygw Copy   

•   Source:

Authors: yliu
Group: OnsiteGene 1, XPRIZE Rapid Covid Testing
Summary: This OnsiteGene protocol is designed for testing the normal saliva samples with nucleic acid extraction. A normal saliva collection tube is used for the collection. The protocol uses the Star Array® Hi-SenseTM COVID-19 Molecular Testing Kit 1.0 in the one-step real-time RT-qPCR test assay to qualitatively detect RNA from SARS-CoV-2 virus in the human respiratory specimen. It combines the reverse transcription technology and real-time PCR method to provide accurate detection of the SARS-CoV-2 coronavirus. The Star Array® magnetic beads RNA extraction instrument and kit can automatically extract and purify the RNA for 32 samples in 6 minutes. It is ideal for the saliva samples that contain high amount of PCR inhibitors and can't be processed by the direct amplification. The triplex fluorescence design of the kit simultaneously detects the N1 and Orf1ab genes of the virus and the human RNase P gene as an internal control to ensure the sample quality. This protocol uses the Star Array® XDiveTM Superfast Real-Time PCR instrument to perform 40-cycle PCR in 8 minutes, and can test up to 16 samples or controls in each run. The total protocol time from sample collection to data interpretation is less than 15 minutes.The sample collection steps is adapted from the published protocol:https://www.protocols.io/view/saliva-collection-and-rna-extraction-for-sars-cov-bh6mj9c6

Proper citation: yliu 2020. OnsiteGene 4 Protocol Saliva Extract. protocols.io https://dx.doi.org/10.17504/protocols.io.bpjemkje Copy   

•   Source:

Authors: steve.puts
Group: Coronavirus Method Development Community, Mmolecular, XPRIZE Rapid Covid Testing
Summary: The Direct One-Step RT-qPCR COVID-19 Test Kit is designed for quantitativereal-time analysis of target RNA directly from, swabs without the requirement of any priorRNA purification steps.Shipping: shipped on blue ice Storage Conditions: store at -20 °C Additional Storage Conditions: avoid freeze/thaw cycles stable at 4 °C for up to 4 weeks Shelf Life: 12 monthsForm: liquid / lyophilized

Proper citation: steve.puts 2020. Mmolecular COVID-19 Extraction-Free Direct-One-Step Fast Cycling Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bkjpkumn Copy   

•   Source:

Authors: Jon Arizti-Sanz, Catherine A. Freije, Chloe K. Boehm, Sameed M. Siddiqui, Allen M. Goodman, Tinna-Solveig F. Kosoko-Thoroddsen, A'Doriann Y. Bradley, Jeremy Johnson, Pardis C. Sabeti, Cameron Myhrvold
Group: XPRIZE Rapid Covid Testing
Summary: This protocol describes how to perform a SHINE in-tube fluorescent assay to detect SARS-CoV-2 RNA from a self-collected nasopharyngeal sample. This protocol is intended for point-of-care use. All enzymatic components are provided as a single-test freeze-dried pellet for shelf-stable storage, and all steps of the protocol are performed at ambient temperature. The protocol requires a transilluminator or another equivalent blue light emitting device. The protocol presented here is an improved version of the method presented in Arizti-Sanz J*, Freije CA*, et al. Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2. bioRxiv (2020).

Proper citation: Jon Arizti-Sanz, Catherine A. Freije, Chloe K. Boehm, Sameed M. Siddiqui, Allen M. Goodman, Tinna-Solveig F. Kosoko-Thoroddsen, A'Doriann Y. Bradley, Jeremy Johnson, Pardis C. Sabeti, Cameron Myhrvold 2020. XPRIZE SHINE - In-tube Fluorescent SARS-CoV-2 NP Test. protocols.io https://dx.doi.org/10.17504/protocols.io.bk4hkyt6 Copy   

•   Source:

Authors: vincent.degennaro
Group: API Pharma LLC, XPRIZE Rapid Covid Testing

Proper citation: vincent.degennaro 2020. mariPOC User manual.5.5.20. protocols.io https:// Copy   

•   Source:

Authors: David Levesque
Group: XPRIZE Rapid Covid Testing

Proper citation: David Levesque 2020. vBio Voice Analysis. protocols.io https:// Copy   

•   Source:

Authors: Miriam Schwartz, Nikhil Ponon, Caleb Stewart, Luke Saban, Jody Ranck
Group: Ram Global, XPRIZE Rapid Covid Testing
Summary: RAM Group has developed ThEA™ - an optical, reagent free / chemical free detection system for rapid diagnosis for SARS-Cov-2. The system consists of a THz spectrometer, a low-cost disposable sensor cartridge and a set of classification algorithms. ThEA’s The test includes taking an oropharyngeal swab from the patient and directly smearing it on the sensor surface followed by reading out the THz response in a THz time domain spectrometer. ThEA analysis software with classification algorithm will then be able to classify whether the sample is infectious with Sars-Cov-2 virus or not.

Proper citation: Miriam Schwartz, Nikhil Ponon, Caleb Stewart, Luke Saban, Jody Ranck 2020. ThEA Direct Swab . protocols.io https://dx.doi.org/10.17504/protocols.io.bkh4kt8w Copy   

•   Source:

Authors: Monica Simion, Alexandru Salceanu
Group: Coronavirus Method Development Community, covid19-test, XPRIZE Rapid Covid Testing

Proper citation: Monica Simion, Alexandru Salceanu 2020. microfluidic sars cov 2 lamp protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bkjjkukn Copy   

•   Source:

Authors: John Daniels
Group: Coronavirus Method Development Community, Front Line Technologies, XPRIZE Rapid Covid Testing, Team Exhaled Breath Condensate
Summary: The Kinaptic mask-based COVID-19 testing system uses a unique Exhaled Breath Condensate (EBC) collector that converts breath vapor to a liquid biosample for rapid testing and wireless automatic contact tracing. Exhaled Breath Condensate (EBC) is collected from a mask-based EBC collector as a low cost disposable component. Our electronic biosensor detects even low concentrations of virus biomarkers if they are present in the EBC sample. Corona viruses have slowly evolving N-protein and quickly evolving S-protein. N-Protein testing screens for any corona virus infection / S-Protein screens for only COVID-19 infection. The Kinaptic mask-based COVID-19 testing system is a “two-stage filter”: 1) catch only of N-protein biomarker = trigger warning to get more stringent diagnostic and contact trace; 2) catch both N- and S-, or just S- biomarkers = more urgent need for immediate quarantine and medical attention. As the target biomarkers bind to the capture molecules, the electrical conductivity of the electronic biosensor changes. This change in conductive is amplified by our bluetooth electronics, analyzed to determine the presence and quantity of the target biomarker, and the test result wireless transmitted to a smartphone or directly to the cloud.

Proper citation: John Daniels 2020. Mask-Based Covid-10 testing system using Exhaled Breath Condensate. protocols.io https://dx.doi.org/10.17504/protocols.io.bkzzkx76 Copy   

•   Source:

Authors: Ruth Polachek, doreen
Group: XPRIZE Rapid Covid Testing
Summary: LessTests SARS-CoV2 general diagnosis protocol, using LessTests software solution.The LessTests method is agnostic to the labware, and has already been demonstrated as successful using multiple device and kit combinations.

Proper citation: Ruth Polachek, doreen 2020. LessTests. protocols.io https://dx.doi.org/10.17504/protocols.io.bkiakuae Copy   

•   Source:

Authors: Mario Thomas, Jasmine Sollen, Natalia Ivanova, Michelle Feng, Reda Fayek, Heidi Abdilla, Stephanie Lim, Amanda Naaum
Group: Coronavirus Method Development Community, XPRIZE Rapid Covid Testing, SANATA
Summary: This procedure outlines the protocol for testing for SARS-CoV-2 using a saliva sample collected from an individual. The purpose of this test is to detect low levels of SARS-CoV-2 antigen at a higher sensitivity. Precision Biomonitoring Inc. developed an ultra-rapid digital, disposable, highly-sensitive and inexpensive testing device used for screening purposes. The mobile app complementary to this medical device is connected through Bluetooth. Using this innovation, the user can be tested at point-of-care (POC) by a health care professional, and obtain qualitative results.

Proper citation: Mario Thomas, Jasmine Sollen, Natalia Ivanova, Michelle Feng, Reda Fayek, Heidi Abdilla, Stephanie Lim, Amanda Naaum 2020. XPRIZE SANATA Protocol for Saliva LFIA Test. protocols.io https://dx.doi.org/10.17504/protocols.io.bqfrmtm6 Copy   

•   Source:

Authors: vincent.degennaro
Group: Coronavirus Method Development Community, API Pharma LLC, XPRIZE Rapid Covid Testing

Proper citation: vincent.degennaro 2020. API Pharma Next Gen Antibody Test. protocols.io https://dx.doi.org/10.17504/protocols.io.bjqqkmvw Copy   

•   Source:

Authors: , marcello.manfredi , Paolo Satta, Diogo Barros, Isabel Correa
Group: XPRIZE Rapid Covid Testing

Proper citation: , marcello.manfredi , Paolo Satta, Diogo Barros, Isabel Correa 2020. transmissive blood test. protocols.io https://dx.doi.org/10.17504/protocols.io.bj2pkqdn Copy   

•   Source:

Authors: Diego Lai
Group: Coronavirus Method Development Community, XPRIZE Rapid Covid Testing
Summary: LooK SPOT COVID-19 antigen rapid test is a lateral flow immunoassay intended for the qualitative detection of nucleocapsid protein antigen from SARS-CoV-2 in nasal swabs from patients suspected of COVID-19 within the first seven (7) days of symptom onset. Testing is limited to laboratories certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA), 42 U.S.C. §263a, that meet the requirements to perform moderate complexity tests. This test is authorized for use at the Point of Care (POC), i.e., in a patient care settings operating under a CLIA Certificate of Waiver, Certificate of Compliance, or Certificate of Accreditation.].Results are for the identification of SARS-CoV-2 nucleocapsid protein antigen. The antigen is generally detectable in nasal samples during the acute phase of infection. Positive results indicate the presence of viral antigens, but the clinical correlation with patient history and other diagnostic information is necessary to determine infection status. Positive results do not rule out a bacterial infection or co-infection with other viruses. The agent detected may not be the definite cause of thedisease. Laboratories within the United States and its territories are required to report all positive results to the appropriate public health authorities.Negative results should be treated as presumptive, and do not rule out SARS-CoV-2 infection, and should not be used as the sole basis for treatment or patient management decisions, including infection control decisions. Negative results should be considered in the context of a patient's recent exposures, history, and the presence of clinical signs and symptoms consistent with COVID-19, and confirmed with a molecular assay, if necessary, for patient management. LooK SPOT COVID-19 Antigen Rapid Test can deliver a diagnosis of the SARS-CoV-2 virus detection between 5 to 8 minutes by using machine learning AI. LooK SPOT’s AI algorithm has high accuracy and can identify the fluorescence response when human eyes cannot identify the low positive cases. Healthcare responders in the COVID-19 test sites often need to make time-sensitive decisions to determine the test results during the time many patients are within their vicinity. But the tempo, volume, stress, fatigue, lighting, fear, and various other factors can overwhelm healthcare responders when making the visual interpretation of antigen test results. It is of paramount importance to reduce healthcare responders' cognitive load by providing accurate test results in an easy-to-read format. LooK SPOT COVID-19 Antigen Rapid Test is a COVID-19 rapid test solution designed with a tactical edge to fight COVID-19. The LooK SPOT COVID-19 Antigen Rapid Test is intended for use at the Point of Care (POC) settings by trained personnel specifically instructed and trained in vitro diagnostic procedures. It is only for use under the Food and Drug Administration's Emergency Use Authorization.

Proper citation: Diego Lai 2020. LooK SPOT Antigen Rapid Test System. protocols.io https://dx.doi.org/10.17504/protocols.io.bp32mqqe Copy   

•   Source: