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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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MojoSort™ Whole Blood Human Neutrophil Isolation Kit Protocol Resource Report Resource Website |
Ken Lau | BioLegend | BioLegend | https://www.biolegend.com/en-us/protocols/mojosort-whole-blood-human-neutrophil-isolation-kit-protocol | 1 | 2021 | Ken Lau 2021. MojoSort™ Whole Blood Human Neutrophil Isolation Kit Protocol. protocols.io https:// | 2021-04-15 09:15:21 | ||||
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Immunofluorescence Microscopy Protocol Resource Report Resource Website |
BioLegend, Inc. | DOI:10.17504/protocols.io.hv8b69w | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/Immunofluorescence_Microscopy_Protocol_050514.pdf | 2 | 2017 | BioLegend, Inc. 2017. Immunofluorescence Microscopy Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.hv8b69w | 2021-04-15 09:15:22 | |||
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Anti-Neu5Gc Antibody Kit Protocol - Flow Cytometry Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.baaqiadw | BioLegend | Anti-Neu5Gc may be used for staining cells prior to analysis by Flow Cytometry. This kit contains all the essential components needed to identify Neu5Gc on the surface of cells by flow cytometry.Use of the blocking agent (Neu5Gc Assay Blocking Solution) provided in the kit is essential, as commonly used blocking agents invariably contain serum, or serum components, that can either inhibit detection or introduce Neu5Gc contamination.Tissue culture-grown CHO-K1 cells can be used as a positive control, and human peripheral blood mononuclear cells serve as a negative control. Adherent tissue culture-grown cells should be released from the culture flasks by using 5-10mM EDTA for 10 minutes at room temperature. Other non-enzymatic methods, such as Accutase®, may be used. Immediately wash cells in blocking buffer that contains a lower concentration of EDTA, and resuspend cells in blocking buffer to determine cell numbers and viability.It is assumed that the user is familiar with the general principles and practices of Flow Cytometry. | BioLegend | https://www.biolegend.com/protocols/anti-neu5gc-antibody-kit-protocol-flow-cytometry/4264/ | 3 | 2019 | Sam Li 2019. Anti-Neu5Gc Antibody Kit Protocol - Flow Cytometry. protocols.io https://dx.doi.org/10.17504/protocols.io.baaqiadw | 2021-04-15 09:15:49 | ||
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Immunohistochemistry Protocol for Sternberger Monoclonal Antibodies Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e2jbgcn | BioLegend | BioLegend | http://www.biolegend.com/media_assets/support_protocol/Sternberger_Monoclonals_IHC_Protocol_using_USA_Kit_102914.pdf | 1 | 2016 | Kelsey Miller 2016. Immunohistochemistry Protocol for Sternberger Monoclonal Antibodies. protocols.io https://dx.doi.org/10.17504/protocols.io.e2jbgcn | 2021-04-15 09:15:26 | |||
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MojoSort™ Human CD14+ Monocyte Isolation Kit Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bbwaipae | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-cd14--monocyte-isolation-kit-column-protocol/4764/ | 2 | 2020 | Sam Li 2020. MojoSort™ Human CD14+ Monocyte Isolation Kit Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bbwaipae | 2021-04-15 09:15:25 | ||
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MojoSort™ Human anti-APC Nanobeads Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7x2hpqe | BioLegend | Product description and procedure summary:Target cells are positively selected or depleted by incubating the sample with an anti-human APC conjugated antibody, followed by incubation with magnetic anti-APC Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. These are the APC+ cells, do not discard them if those are the cells of interest. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Scale up volumes if using 14 mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-anti-apc-nanobeads-protocol/4755/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human anti-APC Nanobeads Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7x2hpqe | 2021-04-15 09:15:32 | ||
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Cell Fixation and Permeabilization Protocol using 70% Ethanol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bacziax6 | BioLegend | BioLegend | https://www.biolegend.com/protocols/cell-fixation-and-permeabilization-protocol-using-70-ethanol/4252/ | 3 | 2019 | Sam Li 2019. Cell Fixation and Permeabilization Protocol using 70% Ethanol. protocols.io https://dx.doi.org/10.17504/protocols.io.bacziax6 | 2021-04-15 09:15:50 | |||
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MojoSort™ Streptavidin Nanobeads Column Protocol - Negative Selection Resource Report Resource Website |
Kelsey Miller, Sam Li | DOI:10.17504/protocols.io.693hh8n | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend, BioLegend | https://www.biolegend.com/protocols/mojosort-streptavidin-nanobeads-column-protocol-negative-selection/4772/ | 2 | 2019 | Kelsey Miller, Sam Li 2019. MojoSort™ Streptavidin Nanobeads Column Protocol - Negative Selection. protocols.io https://dx.doi.org/10.17504/protocols.io.693hh8n | 2021-04-15 09:15:51 | ||
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MojoSort™ Nanobeads Protocol - 1 Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7u5hny6 | BioLegend | Product description and procedure summary:The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14 mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-nanobeads-protocol-1/4603/ | 1 | 2019 | Sam Li 2019. MojoSort™ Nanobeads Protocol - 1. protocols.io https://dx.doi.org/10.17504/protocols.io.7u5hny6 | 2021-04-15 09:15:40 | ||
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TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 3.1 Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.8aahsae | BioLegend | Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. TotalSeq™-B antibodies are designed to be used with 10x Single Cell 3’ Reagent Kit v3 with Feature Barcoding. The adjusted protocol below is for customers who are utilizing TotalSeq™-A antibodies with the v3 kit instead. Note: Step 4 from the 10x Genomics user guide document number CG000185, Rev B only applies to the cDNA library and NOT to the TotalSeq™-A library. Please read the entire protocol below and the 10x Genomics user guide for the Chromium Single Cell 3ʹ Reagent Kits v3 with Feature Barcoding technology for Cell Surface Protein before starting the experiments. 10x Genomics user guide document number CG000185, Rev B. Commonly used abbreviations:ADT: Antibody derived tags, the oligo sequence conjugated to regular TotalSeq™-A antibodiesHTO: Hashtag oligonucleotides, the oligo sequence conjugated to TotalSeq™-A Hashtag antibodies | BioLegend | https://www.biolegend.com/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-31-protocol/5010/ | 1 | 2019 | Sam Li 2019. TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 3.1 Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.8aahsae | 2021-04-15 09:15:40 | ||
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MojoSort™ Nanobeads Regular Protocol Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e2sbgee | BioLegend | The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: For Human CD4 Nanobeads, please refer to the protocol found on the product webpage for MojoSort™ Human CD4 Nanobeads (Cat. No. 480013/480014). | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Nanobeads_Regular_Protocol_V02_10192015.pdf | 1 | 2016 | Kelsey Miller 2016. MojoSort™ Nanobeads Regular Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.e2sbgee | 2021-04-15 09:15:40 | ||
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Sandwich ELISA Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.98xh9xn | BioLegend | BioLegend | https://www.biolegend.com/protocols/sandwich-elisa-protocol/4268/ | 3 | 2019 | Sam Li 2019. Sandwich ELISA Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.98xh9xn | 2021-04-15 09:15:43 | |||
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Immunoprecipitation Protocol Resource Report Resource Website |
Kelsey Knight | DOI:10.17504/protocols.io.tkmeku6 | BioLegend | Immunoprecipitation is a procedure by which proteins or peptides that react specifically with an antibody are removed from solution and examined for quantity or physical characteristics. Immunoprecipitation can also be used to “enrich” a protein population prior to Western Blotting. For example, one can perform immunoprecipitation with a pan-specific antibody against a protein of interest followed by Western blotting with a modification-specific antibody (such as a phospho-specific antibody or an acetylation-specific antibody). | BioLegend | https://www.biolegend.com/protocols/immunoprecipitation-protocol/4258/ | 2 | 2018 | Kelsey Knight 2018. Immunoprecipitation Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.tkmeku6 | 2021-04-15 09:15:54 | ||
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MojoSort™ Human anti-APC Nanobeads Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7afhibn | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-anti-apc-nanobeads-column-protocol/4758/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human anti-APC Nanobeads Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7afhibn | 2021-04-15 09:15:41 | ||
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Activation and Intracellular Staining of Whole Blood: For the Detection of Intracellular Cytokines and Other Intracellular Targets Resource Report Resource Website |
BioLegend, Inc. | DOI:10.17504/protocols.io.hukb6uw | BioLegend | This is part of BioLegend's, "Intracellular Flow Cytometry Staining Protocol: For the Detection of Intracellular Cytokines and Other Intracellular Targets". | , | http://www.biolegend.com/media_assets/support_protocol/Intracellular_Staining_Protocol_082615.pdf | 2 | 2017 | BioLegend, Inc. 2017. Activation and Intracellular Staining of Whole Blood: For the Detection of Intracellular Cytokines and Other Intracellular Targets. protocols.io https://dx.doi.org/10.17504/protocols.io.hukb6uw | 2021-04-15 09:15:55 | ||
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MojoSort™ Human CD4 T Cell Selection Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7z8hp9w | BioLegend | Product description and procedure summary: This kit is designed for the sequential positive selection of CD4+ T cells from human peripheral blood mononuclear cells (PBMCs). Human monocytes express both CD14 and CD4. When using only CD4 Nanobeads for positive selection of human CD4 T cells, monocytes could be isolated along with the T cells. If this monocyte fraction does not impact your application, there is no need to address it. However, not including this population may be required. Thus, the first step in this kit is the depletion of CD14+ cells using a combination of biotin anti-human CD14 and Streptavidin Nanobeads. The second step is the positive selection of the CD4 T cells using directly conjugated CD4 Nanobeads. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-cd4-t-cell-selection-protocol/4279/ | 2 | 2019 | Sam Li 2019. MojoSort™ Human CD4 T Cell Selection Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7z8hp9w | 2021-04-15 09:15:51 | ||
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MojoSort™ Streptavidin Nanobeads Protocol Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e2rbgd6 | BioLegend | The cells targeted by the Streptavidin Nanobeads are either selected or depleted by incubating your sample with the magnetic particles after incubating with a biotin-conjugated antibody or antibody cocktail. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Streptavidin_Nanobeads_Protocol_V02_10192015.pdf | 1 | 2016 | Kelsey Miller 2016. MojoSort™ Streptavidin Nanobeads Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.e2rbgd6 | 2021-04-15 09:16:02 | ||
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MojoSort™ Streptavidin Nanobeads Column Protocol - Positive Selection Resource Report Resource Website |
Kelsey Miller, Sam Li | DOI:10.17504/protocols.io.692hh8e | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend, BioLegend | https://www.biolegend.com/protocols/mojosort-streptavidin-nanobeads-column-protocol-positive-selection/4773/ | 2 | 2019 | Kelsey Miller, Sam Li 2019. MojoSort™ Streptavidin Nanobeads Column Protocol - Positive Selection. protocols.io https://dx.doi.org/10.17504/protocols.io.692hh8e | 2021-04-15 09:16:03 | ||
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MojoSort™ Mouse anti-APC Nanobeads Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7xhhpj6 | BioLegend | Product description and procedure summary: Target cells are positively selected or depleted by incubating the sample with an anti-human APC conjugated antibody, followed by incubation with magnetic anti-APC Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. These are the APC+ cells, do not discard them if those are the cells of interest. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-anti-apc-nanobeads-protocol/4756/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse anti-APC Nanobeads Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7xhhpj6 | 2021-04-15 09:15:59 | ||
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MojoSort™ Mouse CD8a Selection Kit Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bbugintw | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer. | BioLegend | https://www.biolegend.com/en-us/protocols/mojosort-mouse-cd8a-selection-kit-column-protocol | 1 | 2020 | Sam Li 2020. MojoSort™ Mouse CD8a Selection Kit Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bbugintw | 2021-04-15 09:16:01 |
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