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| Name | Authors | DOI | Group |
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RRIDs used | ||||||
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ELISA for quantification of IL-10 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjy4kpyw | University of the West Indies, [email protected] | Interleukin-10 is a pleiotropic cytokine playing a critical role as a regulator of myeloid and lymphoid cell function. Due to the ability of IL-10 to blocking cytokine synthesis and many accessory cell functions of antigen-presenting cells such as macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. IL-10 also participates in regulating proliferation and differentiation of B-lymphocytes, mast cells and thymocytes [1]. Reference1. Ouyang W, O'Garra A. IL-10 Family Cytokines IL-10 and IL-22: from Basic Science to Clinical Translation.Immunity. 2019;50(4):871-891. doi:10.1016/j.immuni.2019.03.020 | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-10 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bjy4kpyw | 2021-03-29 03:12:53 | |||
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Indirect ELISA for detection of anti-HIV antibodies in cats immunized with a HIV gp120 peptide. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjnpkmdn | University of the West Indies, [email protected] | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Indirect ELISA for detection of anti-HIV antibodies in cats immunized with a HIV gp120 peptide.. protocols.io dx.doi.org/10.17504/protocols.io.bjnpkmdn | 2021-03-29 03:12:53 | ||||
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Universal sandwich ELISA for investigating the binding of avian and mammalian immunoglobulins to Streptococcal protein-G (SpG) using a peroxidase-labeled Protein LAG conjugate (SpLAG-HRP). Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjsfknbn | University of the West Indies, [email protected] | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Universal sandwich ELISA for investigating the binding of avian and mammalian immunoglobulins to Streptococcal protein-G (SpG) using a peroxidase-labeled Protein LAG conjugate (SpLAG-HRP).. protocols.io dx.doi.org/10.17504/protocols.io.bjsfknbn | 2021-03-29 03:14:29 | ||||
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Universal sandwich ELISA for investigating the binding of Protein L to avian immunoglobulins using anti-IgY-peroxidase as conjugate. Resource Report Resource Website |
Angel Justiz-Vaillant, Monica F. Smikle | 10.17504/protocols.io.bjqykmxw | University of the West Indies, [email protected] | University of the West Indies St. Augustine, University of the West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Monica F. Smikle 2020. Universal sandwich ELISA for investigating the binding of Protein L to avian immunoglobulins using anti-IgY-peroxidase as conjugate.. protocols.io dx.doi.org/10.17504/protocols.io.bjqykmxw | 2021-03-29 03:14:38 | ||||
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Universal sandwich enzyme linked immunosorbent assay for investigating protein-LA (SpLA) interactions with immunoglobulins using a SpG-HRP conjugate. Resource Report Resource Website |
Angel Justiz-Vaillant, Norma McFarlane-Anderson | 10.17504/protocols.io.bjpzkmp6 | University of the West Indies, [email protected] | University of the West Indies St. Augustine, University of West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Universal sandwich enzyme linked immunosorbent assay for investigating protein-LA (SpLA) interactions with immunoglobulins using a SpG-HRP conjugate.. protocols.io dx.doi.org/10.17504/protocols.io.bjpzkmp6 | 2021-03-29 03:14:38 | ||||
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ELISA for quantification of IL-32 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj35kqq6 | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. IL-32 is a pro-inflammatory molecule. Natural killer cells and monocytes mainly produce it. IL-32 induces the synthesis of various cytokines including IL-6, and IL-1beta. It inhibits IL-15 production. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-32 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj35kqq6 | 2021-03-29 03:14:38 | |||
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Preparation of horseradish peroxidase (HRP) conjugated Peptostreptococcal protein-L by the periodate method. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj5ukq6w | University of the West Indies, [email protected] | This reagent can be used in ELISA, Western blotting and Dot blot to detect antigens and antibodies. It is important in the immunodiagnosis of infectious diseases and other problems. Protein L binds to kappa light chains of immunoglobulins from many animal species including human, mouse, rat, chicken, hamster and pig [1].1. Justiz-Vaillant AA, Akpaka PE, McFarlane-Anderson N, Smikle MF. Comparison of techniques of detecting immunoglobulin-binding protein reactivity to immunoglobulin produced by different avian and mammalian species.West Indian Med J. 2013;62(1):12-20. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Preparation of horseradish peroxidase (HRP) conjugated Peptostreptococcal protein-L by the periodate method.. protocols.io dx.doi.org/10.17504/protocols.io.bj5ukq6w | 2021-03-29 03:14:41 | |||
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Preparation of a protein-LA conjugated to horseradish peroxidase by the periodate method. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjkzkkx6 | University of the West Indies, [email protected] | Protein LA, a novel hybrid protein, structurally contains 4 of the Ig Fc-binding and 4 of the Ig Fab binding regions on SpA with 4 of kappa light chain-binding sites of protein L. It has a MW of 65 kDa. Protein LA combines the binding properties of the both SpL and SpA and in some cases, give higher binding affinity than either protein alone. The binding of an Ig to SpL does not interfere with binding of another Ig molecule to the SpA domains and vice versa. Protein LA has been shown to bind effectively to immunoglobulins and their fragments from many species of animals [1]. Reference1. Justiz-Vaillant AA, Akpaka PE, McFarlane-Anderson N, Smikle MF. Comparison of techniques of detecting immunoglobulin-binding protein reactivity to immunoglobulin produced by different avian and mammalian species.West Indian Med J. 2013;62(1):12-20. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Preparation of a protein-LA conjugated to horseradish peroxidase by the periodate method.. protocols.io dx.doi.org/10.17504/protocols.io.bjkzkkx6 | 2021-03-29 03:14:59 | |||
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ELISA for quantification of IL-31 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bkemktc6 | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. IL-31 is produced mainly by Th2 cells and dendritic cells. It is a proinflammatory cytokine and a chemotactic factor that direct polymorphonuclear cells, monocytes, and T cells to inflammatory lesions. IL-31 induces chemokines production and synthesis of IL-6, IL-16, and IL-32. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-31 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bkemktc6 | 2021-03-29 03:12:59 | |||
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Enzyme linked immunosorbent assay (ELISA) for determining the serum concentration of IL-17 in humans. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjyykpxw | University of the West Indies, [email protected] | Interleukin17A is a pro-inflammatory cytokine. This protein is produced by a group of T helper cell known as T- helper17 lymphocytes in response to their stimulation with IL-23. After interaction with its receptor, IL-17 activates signalling pathways that stimulate the production of chemokines [1 ].Reference1. Cho ML, Kang JW, Moon YM, Nam HJ, Jhun JY, Heo SB, Jin HT, Min SY, Ju JH, Park KS, Cho YG, Yoon CH, Park SH, Sung YC, Kim HY (May 2006). Journal of Immunology. 176 (9): 5652–61. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Enzyme linked immunosorbent assay (ELISA) for determining the serum concentration of IL-17 in humans.. protocols.io dx.doi.org/10.17504/protocols.io.bjyykpxw | 2021-03-29 03:13:00 | |||
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Sandwich ELISA for investigating the binding of Protein-LG (SpLG) to avian immunoglobulins using anti-IgY-peroxidase as conjugate. Resource Report Resource Website |
Angel Justiz-Vaillant, Monica F. Smikle | 10.17504/protocols.io.bjq4kmyw | University of the West Indies, [email protected] | University of the West Indies St. Augustine, University of the West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Monica F. Smikle 2020. Sandwich ELISA for investigating the binding of Protein-LG (SpLG) to avian immunoglobulins using anti-IgY-peroxidase as conjugate.. protocols.io dx.doi.org/10.17504/protocols.io.bjq4kmyw | 2021-03-29 03:13:13 | ||||
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ELISA for quantification of IL-25 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj3tkqnn | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Dendritic cells produced predominantly IL-25. It acts on various types of cells, including Th2 cells. It stimulates the synthesis of Th2 cytokine profile including IL-4 and IL-13. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-25 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj3tkqnn | 2021-03-29 03:13:10 | |||
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Enzyme linked immunosorbent assay (ELISA) for determining the serum concentration of IL-23 in humans. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjy2kpye | University of the West Indies, [email protected] | Interleukin 23 activates the production of IL-17A, which is a pro-inflammatory cytokine, produced by a group of T helper cell known as T- helper17 lymphocytes in response to the said IL-23 [1,2].Reference1. Cho ML, Kang JW, Moon YM, Nam HJ, Jhun JY, Heo SB, Jin HT, Min SY, Ju JH, Park KS, Cho YG, Yoon CH, Park SH, Sung YC, Kim HY (May 2006). Journal of Immunology. 176 (9): 5652–61. 2. Nobee A, Justiz-Vaillant A, Akpaka PE, Poon-King P (2016) Levels of Interleukin 17 and 23 in Patients with Systemic Lupus Erythematosus (SLE) in Trinidad and Tobago. Immunochem Immunopathol 2: 115. doi: 10.4172/2469- 9756.1000115 | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Enzyme linked immunosorbent assay (ELISA) for determining the serum concentration of IL-23 in humans.. protocols.io dx.doi.org/10.17504/protocols.io.bjy2kpye | 2021-03-29 03:13:14 | |||
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Enzyme linked immunosorbent assay for investigating the binding of chemically prepared protein-LAG-anti-IgY (SpLAG-anti-IgY) to avian and mammalian immunoglobulins. Resource Report Resource Website |
Angel Justiz-Vaillant, Norma McFarlane-Anderson | 10.17504/protocols.io.bjpskmne | University of the West Indies, [email protected] | This SpLAG-anti-IgY ELISA can be used to detect specific antibodies in various animal species including human, goat, donkey, mouse, rat, dog, cow, horse, ostrich, duck, pigeon, bantam hen, rabbit, chicken, monkey, pig, hamster, and many birds, wild and zoo animals species [1].1. Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175 | University of the West Indies St. Augustine, University of West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Enzyme linked immunosorbent assay for investigating the binding of chemically prepared protein-LAG-anti-IgY (SpLAG-anti-IgY) to avian and mammalian immunoglobulins. . protocols.io dx.doi.org/10.17504/protocols.io.bjpskmne | 2021-03-29 03:13:55 | |||
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ELISA for quantification of Vascular endothelial growth factor A (VEGFA) in cell culture supernatant, human serum or plasma Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | 10.17504/protocols.io.bkttkwnn | University of the West Indies, [email protected] | University of the West Indies St. Augustine, "Saturnino Lora Torres" Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of Vascular endothelial growth factor A (VEGFA) in cell culture supernatant, human serum or plasma. protocols.io dx.doi.org/10.17504/protocols.io.bkttkwnn | 2021-03-29 03:14:01 | ||||
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Determination of IgA concentration by the Mancini test. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj93kr8n | University of the West Indies, [email protected] | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Determination of IgA concentration by the Mancini test.. protocols.io dx.doi.org/10.17504/protocols.io.bj93kr8n | 2021-03-29 03:14:10 | ||||
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ELISA for quantification of IL-40 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj4fkqtn | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. IL-40 is produced in the bone marrow, fetal liver, and by activated B cells. IL-40 plays a vital role in the development of humoral immune responses. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-40 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj4fkqtn | 2021-03-29 03:14:17 | |||
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Feeding of cats with hyper-immune eggs: an oral immunization protocol Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjnnkmde | University of the West Indies, [email protected] | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Feeding of cats with hyper-immune eggs: an oral immunization protocol. protocols.io dx.doi.org/10.17504/protocols.io.bjnnkmde | 2021-03-29 03:14:22 | ||||
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Universal sandwich enzyme linked immunosorbent assay for investigating immunoglobulin-binding protein (IBP) interactions using a conjugate SpAG-HRP. Resource Report Resource Website |
Angel Justiz-Vaillant, Norma McFarlane-Anderson | 10.17504/protocols.io.bjptkmnn | University of the West Indies, [email protected] | University of the West Indies St. Augustine, University of West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Universal sandwich enzyme linked immunosorbent assay for investigating immunoglobulin-binding protein (IBP) interactions using a conjugate SpAG-HRP.. protocols.io dx.doi.org/10.17504/protocols.io.bjptkmnn | 2021-03-29 03:14:24 | ||||
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ELISA for quantification of IL-15 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj3ekqje | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Monocytes, epithelium, and muscles make IL-15. It acts on T cells and activated B cells. It causes the proliferation of both B and T cells. It causes NK cell memory and CD8+ T cell proliferation. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-15 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj3ekqje | 2021-03-29 03:15:10 |
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