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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
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MojoSort™ Human CD45 Nanobeads Protocol B Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e22bgge | Note: If the percentage of CD45+ cells in your sample is less than 50%, please follow Protocol A. If it is higher than 50% then please follow protocol B.The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Human_CD45_Nanobeads_Protocol_12182015.pdf | 1 | 2016 | Kelsey Miller 2016. MojoSort™ Human CD45 Nanobeads Protocol B. protocols.io https://dx.doi.org/10.17504/protocols.io.e22bgge | 2021-04-15 09:16:29 | |||
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ELISPOT Protocol Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e29bgh6 | BioLegend | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BioLegend_ELISPOT_protocol_111511.pdf | 1 | 2016 | Kelsey Miller 2016. ELISPOT Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.e29bgh6 | 2021-04-15 09:16:57 | |||
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Th9 Polarization of Mouse CD4+ Cells Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e2cbgaw | BioLegend | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BioLegend_Th9_Polarization_of_Mouse_CD4_Cells_012715.pdf | 1 | 2016 | Kelsey Miller 2016. Th9 Polarization of Mouse CD4+ Cells. protocols.io https://dx.doi.org/10.17504/protocols.io.e2cbgaw | 2021-04-15 09:16:44 | |||
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Anti-BrdU Staining Using 70% Ethanol and 2N HCl Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.hvmb646 | BioLegend | Anti-BrdU Staining Protocol Using 70% Ethanol and 2N HCl | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BrdU_Staining_Protocols_05102016.pdf | 2 | 2017 | Kelsey Miller 2017. Anti-BrdU Staining Using 70% Ethanol and 2N HCl. protocols.io https://dx.doi.org/10.17504/protocols.io.hvmb646 | 2021-04-15 09:16:44 | ||
|
MojoSort™ Human CD45 Nanobeads Protocol A Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e2zbgf6 | Note: If the percentage of CD45+ cells in your sample is less than 50%, please follow Protocol A. If it is higher than 50% then please follow protocol B.The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Human_CD45_Nanobeads_Protocol_12182015.pdf | 1 | 2016 | Kelsey Miller 2016. MojoSort™ Human CD45 Nanobeads Protocol A. protocols.io https://dx.doi.org/10.17504/protocols.io.e2zbgf6 | 2021-04-15 09:17:00 | |||
|
MojoSort™ Human CD14 Selection Kit Protocol Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e3jbgkn | BioLegend | Product description and procedure summary:Target cells are either selected or depleted by incubating your sample with the biotin anti-human CD14 antibody (clone 63D3) followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. These are the CD14+ cells, do not discard them if those are your cells of interest. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Human_CD14_Selection_Kit_Protocol_10192015.pdf | 1 | 2016 | Kelsey Miller 2016. MojoSort™ Human CD14 Selection Kit Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.e3jbgkn | 2021-04-15 09:17:02 | ||
|
Immunohistochemistry Protocol for Sternberger Monoclonal Antibodies Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.tkkekuw | BioLegend | BioLegend | https://www.biolegend.com/protocols/immunohistochemistry-protocol-for-sternberger-monoclonal-antibodies/4253/ | 2 | 2018 | Kelsey Miller 2018. Immunohistochemistry Protocol for Sternberger Monoclonal Antibodies. protocols.io https://dx.doi.org/10.17504/protocols.io.tkkekuw | 2021-04-15 09:17:05 | |||
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Anti-BrdU Staining Using 70% Ethanol and 2N HCl Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.tibekan | BioLegend | Anti-BrdU Staining Protocol Using 70% Ethanol and 2N HCl | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BrdU_Staining_Protocols_05102016.pdf | 3 | 2018 | Kelsey Miller 2018. Anti-BrdU Staining Using 70% Ethanol and 2N HCl. protocols.io https://dx.doi.org/10.17504/protocols.io.tibekan | 2021-04-15 09:17:03 | ||
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Immunofluorescent Staining of Foxp3 in Frozen Sections Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.hwbb7an | BioLegend | T regulatory cells (also known as Tregs or Regulatory T cells) are essential cells in the immune system that suppress immune responses of other cells, designed to limit excessive reactions and prevent autoimmunity. Tregs are characterized by the expression of CD4, CD25, and Foxp3, while lacking CD127. CD4+Foxp3+ regulatory T cells have been referred to as “naturally-occurring” regulatory T cells to distinguish them from “suppressor” T cell populations that are generated in vitro. While other variants of suppressive T cells do exist, such as CD8 suppressor cells, Th3 and Tr-1 cells, Tregs are classically defined as CD4+CD25+Foxp3+ cells. Here, we provide a protocol for immunofluorescent staining of FOXP3 in frozen sections, adapted from a protocol from Dr. Matthias Hardtke-Wolenski, Medizinische Hochschule Hannover. This protocol has been successfully used on mouse spleen, liver, and skin sections. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BioLegend_IF-FOXP3_121511.pdf | 2 | 2017 | Kelsey Miller 2017. Immunofluorescent Staining of Foxp3 in Frozen Sections. protocols.io https://dx.doi.org/10.17504/protocols.io.hwbb7an | 2021-04-15 09:17:06 | ||
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Anti-BrdU Staining Using 70% Ethanol and 2N HCl Resource Report Resource Website |
Kelsey Miller | DOI:10.17504/protocols.io.e2pbgdn | BioLegend | Anti-BrdU Staining Protocol Using 70% Ethanol and 2N HCl | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BrdU_Staining_Protocols_05102016.pdf | 1 | 2016 | Kelsey Miller 2016. Anti-BrdU Staining Using 70% Ethanol and 2N HCl. protocols.io https://dx.doi.org/10.17504/protocols.io.e2pbgdn | 2021-04-15 09:17:07 |
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