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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RdRp nested RT-PCR Resource Report Resource Website |
Judy Northill, Ian Mackay | DOI:10.17504/protocols.io.bdcpi2vn | Public Health Virology, Forensic and Scientific Services, Coronavirus Method Development Community | A nested RT-PCR targeting the RdRp region of the sub-genus Sarbecovirus. The primers are modified from the pan-coronavirus RT-PCR published by Hu et al. 2017 to be more specific to SARS-CoV-2.Assay may be used in resource poor settings where real-time cyclers are not available.Sanger sequencing can be used to confirm SARS-CoV-2 where WGS is not available or where WGS fails due to poor quality sample. | Public Health Virology, Forensic and Scientific Services, Public Health Virology, Forensic and Scientific Services | 2 | 2020 | Judy Northill, Ian Mackay 2020. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RdRp nested RT-PCR. protocols.io https://dx.doi.org/10.17504/protocols.io.bdcpi2vn | 2021-04-15 09:17:04 |
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