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Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol describes plasmid cloning by restriction enzyme digest (aka subcloning). To see the full abstract and additional resources, please visit the Addgene protocol page.
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning). protocols.io https://dx.doi.org/10.17504/protocols.io.47ugznw Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This is the protocol accompanying the "pLKO.1 – TRC Cloning Vector". For information about the PLKO.1-TRC cloning vector and tips on designing shRNA oligos for pLKO.1 see Addgene's website: http://www.addgene.org/tools/protocols/plko/
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. Cloning shRNA Oligos into pLKO.1. protocols.io https://dx.doi.org/10.17504/protocols.io.bawmifc6 Copy
Authors: Joanne Kamens, Addgene The Nonprofit Plasmid Repository
Group: Re-Entering Labs Post COVID-19 Shutdown
Summary: Disclaimer: The SOP presented here has been designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational purposes only. If you choose to reuse or repurpose this SOP in another location, please note that you do so at your own risk; you should ensure that any local guidance is also adhered to. None of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to from these web pages.
Proper citation: Joanne Kamens, Addgene The Nonprofit Plasmid Repository 2020. Addgene Covid-19, Back To Work Training. protocols.io https://dx.doi.org/10.17504/protocols.io.bhukj6uw Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: The following protocol is for making LB agar plates for the purpose of bacterial selection (500mL of LB agar makes about 25 LB agar plates). For the full abstract and additional resources, please visit https://www.addgene.org/plasmid_protocols/bacterial_plates/
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. Pouring LB Agar Plates. protocols.io https://dx.doi.org/10.17504/protocols.io.63khgkw Copy
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