Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Artificial Cerebrospinal Fluid VIII (ACSF.VIII) Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bdpai5ie | BICCN, Allen Institute for Brain Science | This protocol describes how to make Artificial Cerebrospinal Fluid VIII (ACSF.VIII). ACSF.VIII is used for multiple applications including incubation of fresh human brain slices prior to electrophysiological recording.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. Artificial Cerebrospinal Fluid VIII (ACSF.VIII). protocols.io https://dx.doi.org/10.17504/protocols.io.bdpai5ie | 2021-04-15 09:15:20 | |||
|
Patch-Seq Recording and Extraction Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bdpei5je | BICCN, Allen Institute for Brain Science | This protocol describes the process to obtain electrophysiological recordings and cellular contents from neurons in postnatal mouse and/or human brain slices.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. Patch-Seq Recording and Extraction. protocols.io https://dx.doi.org/10.17504/protocols.io.bdpei5je | 2021-04-15 09:15:47 | |||
|
Intramuscular Injection Adult Mouse Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bfzfjp3n | BICCN, Allen Institute for Brain Science | This protocol describes the general procedures used for intramuscular injection in adult mice. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. Intramuscular Injection Adult Mouse. protocols.io https://dx.doi.org/10.17504/protocols.io.bfzfjp3n | 2021-04-15 09:15:28 | |||
|
Isolation of Nuelci from Adult_Human_Brain_Tissue Resource Report Resource Website |
LeinU01 BRAIN Grant | DOI:10.17504/protocols.io.y6pfzdn | BICCN, Allen Institute for Brain Science | Isolation of nuclei from frozen adult human brain tissue or thawed and microdissected brain tissue sections for FPCR and/or RNA-seq analysis. | Allen Institute | 1 | 2019 | LeinU01 BRAIN Grant 2019. Isolation of Nuelci from Adult_Human_Brain_Tissue. protocols.io https://dx.doi.org/10.17504/protocols.io.y6pfzdn | 2021-04-15 09:15:25 | |||
|
Zeiss AxioImager Multi Channel 20X Image Capture Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bdpki5kw | BICCN, Allen Institute for Brain Science | This protocol describes the capture of 20X multi-channel images using human and mouse tissue.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. Zeiss AxioImager Multi Channel 20X Image Capture. protocols.io https://dx.doi.org/10.17504/protocols.io.bdpki5kw | 2021-04-15 09:15:31 | |||
|
TissueCyte Troubleshooting Guide Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bbyaipse | BICCN, Allen Institute for Brain Science | This protocol outlines strategies to solve common problems that occur when setting up the TissueCyte 1000 system for normal operation. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. TissueCyte Troubleshooting Guide. protocols.io https://dx.doi.org/10.17504/protocols.io.bbyaipse | 2021-04-15 09:15:50 | |||
|
Leica SP8 Confocal Imaging Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bd8wi9xe | BICCN, Allen Institute for Brain Science | This protocol describes the basic setup and scanning using the Leica SP8 confocal microscope of fluorescently-labeled mouse brain tissue sections mounted on positively charged 1” x 3” microscope slides.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. Leica SP8 Confocal Imaging. protocols.io https://dx.doi.org/10.17504/protocols.io.bd8wi9xe | 2021-04-15 09:15:33 | |||
|
Mouse Cardiac Perfusion Fixation and Brain Collection Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.beudjes6 | BICCN, Allen Institute for Brain Science | This protocol describes the procedures for intracardiac perfusion fixation of postnatal mice, including anesthesia, exsanguination, fixation, brain removal and post-fixation storage. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 3 | 2020 | Allen Institute for Brain Science 2020. Mouse Cardiac Perfusion Fixation and Brain Collection. protocols.io https://dx.doi.org/10.17504/protocols.io.beudjes6 | 2021-04-15 09:15:49 | |||
|
DAB Solutions Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bgndjva6 | BICCN, Allen Institute for Brain Science | 3, 3´ Diaminobenzidine (DAB) is a commonly used chromogen for histological staining. In the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol and xylene. Peroxidase catalyzes hydrogen peroxide oxidation of substrate DAB. This SOP provides instructions to prepare four different formulations of DAB. DAB for Immunohistochemical staining (IHC), DAB for Acetylcholinesterase (AChE) Staining, Nickel Enchanced DAB for Biocytin staining, and DAB for Biocytin staining.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 6 | 2020 | Allen Institute for Brain Science 2020. DAB Solutions. protocols.io https://dx.doi.org/10.17504/protocols.io.bgndjva6 | 2021-04-15 09:15:48 | |||
|
Artificial Cerebrospinal Fluid III (ACSF.III) Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bdpci5iw | BICCN, Allen Institute for Brain Science | Artificial Cerebrospinal Fluid III (ACSF.III) is used for applications including tissue bath solution during electrophysiological recording.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. Artificial Cerebrospinal Fluid III (ACSF.III). protocols.io https://dx.doi.org/10.17504/protocols.io.bdpci5iw | 2021-04-15 09:15:52 | |||
|
Human Post Mortem Brain Processing Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bf4ajqse | BICCN, Allen Institute for Brain Science | This protocol describes the steps for processing post-mortem human brain, starting from whole brain or a single hemisphere.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U01MH114812. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. Human Post Mortem Brain Processing. protocols.io https://dx.doi.org/10.17504/protocols.io.bf4ajqse | 2021-04-15 09:15:52 | |||
|
10xV3 Genomics Sample Processing Protocol Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bq7cmziw | BICCN, Allen Institute for Brain Science | Protocol allows for rapid generation of 3’ transcriptomic-NGS-ready- single-cell-libraries from pools of cells.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2021 | Allen Institute for Brain Science 2021. 10xV3 Genomics Sample Processing Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bq7cmziw | 2021-04-15 09:16:02 | |||
|
Electrode Internal Solution with Biocytin II Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bctsiwne | BICCN, Allen Institute for Brain Science | This SOP provides instructions to prepare Internal Solution II used for simultaneous patch clamp electrophysiology experiments to probe circuit connectivity. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. Electrode Internal Solution with Biocytin II. protocols.io https://dx.doi.org/10.17504/protocols.io.bctsiwne | 2021-04-15 09:16:00 | |||
|
384 Well PicoGreen Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bezejf3e | BICCN, Allen Institute for Brain Science | Quant-iT™ PicoGreen® dsDNA Assay is used for detection and quantitation of double stranded DNA products. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. 384 Well PicoGreen. protocols.io https://dx.doi.org/10.17504/protocols.io.bezejf3e | 2021-04-15 09:16:00 | |||
|
Production of AAV Virus by Iodixanol Gradient Ultracentrifugation Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bdvxi67n | BICCN, Allen Institute for Brain Science | This protocol is used to produce AAV of any serotype with high purity and high titer.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 3 | 2020 | Allen Institute for Brain Science 2020. Production of AAV Virus by Iodixanol Gradient Ultracentrifugation. protocols.io https://dx.doi.org/10.17504/protocols.io.bdvxi67n | 2021-04-15 09:16:00 | |||
|
Fragment Analyzer Operation for PCR Products Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bg4yjyxw | BICCN, Allen Institute for Brain Science | This protocol describes fragment analyzer operation for polymerase chain reaction (PCR) products. The Fragment Analyzer by Advanced Analytical is used as a quality control step for genotyping PCR products. Peak size, shape, and sample concentration are recorded and used to evaluate samples on a pass/fail level.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. Fragment Analyzer Operation for PCR Products. protocols.io https://dx.doi.org/10.17504/protocols.io.bg4yjyxw | 2021-04-15 09:15:59 | |||
|
Slide Preparation for Mounting Samples for TissueCyte 1000 Sectioning and Imaging Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bdvni65e | BICCN, Allen Institute for Brain Science | This protocol describes the preparation of 1x3 glass slides for mounting embedded mouse brain samples for subsequent imaging and sectioning on TissueCyte 1000. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. Slide Preparation for Mounting Samples for TissueCyte 1000 Sectioning and Imaging. protocols.io https://dx.doi.org/10.17504/protocols.io.bdvni65e | 2021-04-15 09:16:04 | |||
|
Digoxigenin Labeled In Vitro Transcription and Ribogreen Quantification Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bg4vjyw6 | BICCN, Allen Institute for Brain Science | This protocol is used for in vitro transcription (IVT) reaction generating DIG-labeled anti-sense (AS) RNA from polymerase chain reaction (PCR) product for in situ hybridization (ISH), as well as fluorescent quantification of products using Ribogreen immediately following. This protocol is written for 96 reactions but can also be used for single reactions or in multiples of 96 by adjusting the amount of materials and equipment appropriately.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. Digoxigenin Labeled In Vitro Transcription and Ribogreen Quantification. protocols.io https://dx.doi.org/10.17504/protocols.io.bg4vjyw6 | 2021-04-15 09:16:08 | |||
|
Patch-Seq Recording and Extraction Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bepyjdpw | BICCN, Allen Institute for Brain Science | This protocol describes the process to obtain electrophysiological recordings and cellular contents from neurons in postnatal mouse and/or human brain slices.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 3 | 2020 | Allen Institute for Brain Science 2020. Patch-Seq Recording and Extraction. protocols.io https://dx.doi.org/10.17504/protocols.io.bepyjdpw | 2021-04-15 09:16:07 | |||
|
Bright Field Image Capture with Zeiss AxioImager Resource Report Resource Website |
Allen Institute for Brain Science | DOI:10.17504/protocols.io.bcsgiwbw | BICCN, Allen Institute for Brain Science | This protocol describes the system that is used to capture Z stack images which are reassembled post-processing into 3D reconstructions. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. Bright Field Image Capture with Zeiss AxioImager. protocols.io https://dx.doi.org/10.17504/protocols.io.bcsgiwbw | 2021-04-15 09:16:06 |
Can't find your Protocol?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific protocol and you know the DOI of the protocol already, it's easier to enter a DOI to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your protocol in the search results, please help us by adding it into the system — it's easy. Create and publish your protocols at Protocols.io.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
