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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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Sandwich ELISA Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.98xh9xn | BioLegend | BioLegend | https://www.biolegend.com/protocols/sandwich-elisa-protocol/4268/ | 3 | 2019 | Sam Li 2019. Sandwich ELISA Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.98xh9xn | 2021-04-15 09:15:43 | |||
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MojoSort™ Human anti-APC Nanobeads Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7afhibn | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-anti-apc-nanobeads-column-protocol/4758/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human anti-APC Nanobeads Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7afhibn | 2021-04-15 09:15:41 | ||
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MojoSort™ Human CD4 T Cell Selection Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7z8hp9w | BioLegend | Product description and procedure summary: This kit is designed for the sequential positive selection of CD4+ T cells from human peripheral blood mononuclear cells (PBMCs). Human monocytes express both CD14 and CD4. When using only CD4 Nanobeads for positive selection of human CD4 T cells, monocytes could be isolated along with the T cells. If this monocyte fraction does not impact your application, there is no need to address it. However, not including this population may be required. Thus, the first step in this kit is the depletion of CD14+ cells using a combination of biotin anti-human CD14 and Streptavidin Nanobeads. The second step is the positive selection of the CD4 T cells using directly conjugated CD4 Nanobeads. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-cd4-t-cell-selection-protocol/4279/ | 2 | 2019 | Sam Li 2019. MojoSort™ Human CD4 T Cell Selection Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7z8hp9w | 2021-04-15 09:15:51 | ||
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MojoSort™ Streptavidin Nanobeads Column Protocol - Positive Selection Resource Report Resource Website |
Kelsey Miller, Sam Li | DOI:10.17504/protocols.io.692hh8e | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend, BioLegend | https://www.biolegend.com/protocols/mojosort-streptavidin-nanobeads-column-protocol-positive-selection/4773/ | 2 | 2019 | Kelsey Miller, Sam Li 2019. MojoSort™ Streptavidin Nanobeads Column Protocol - Positive Selection. protocols.io https://dx.doi.org/10.17504/protocols.io.692hh8e | 2021-04-15 09:16:03 | ||
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MojoSort™ Mouse anti-APC Nanobeads Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7xhhpj6 | BioLegend | Product description and procedure summary: Target cells are positively selected or depleted by incubating the sample with an anti-human APC conjugated antibody, followed by incubation with magnetic anti-APC Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. These are the APC+ cells, do not discard them if those are the cells of interest. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-anti-apc-nanobeads-protocol/4756/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse anti-APC Nanobeads Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7xhhpj6 | 2021-04-15 09:15:59 | ||
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MojoSort™ Mouse CD8a Selection Kit Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bbugintw | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer. | BioLegend | https://www.biolegend.com/en-us/protocols/mojosort-mouse-cd8a-selection-kit-column-protocol | 1 | 2020 | Sam Li 2020. MojoSort™ Mouse CD8a Selection Kit Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bbugintw | 2021-04-15 09:16:01 | ||
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Precision Count Beads™ Protocol and Applications Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.baariad6 | BioLegend | Precision Count Beads™ are designed for counting the absolute number of cells in a complex mix population and other particles by flow cytometry. Precision Count Beads™ are excited by a variety of lasers including violet (405nm), blue (488nm), yellow/green (562nm), and red (633nm). | BioLegend | https://www.biolegend.com/protocols/precision-count-beads-protocol-and-applications/4248/ | 2 | 2019 | Sam Li 2019. Precision Count Beads™ Protocol and Applications. protocols.io https://dx.doi.org/10.17504/protocols.io.baariad6 | 2021-04-15 09:16:06 | ||
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MojoSort™ Mouse CD4 Nanobeads Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.696hh9e | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-cd4-nanobeads-column-protocol/4766/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse CD4 Nanobeads Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.696hh9e | 2021-04-15 09:16:07 | ||
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MojoSort™ Selection Kits Protocol - 5 Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7z9hp96 | BioLegend | Product description and procedure summary:Target cells are either selected or depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads (Cat. No. 480015/480016). The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. If these are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.Sample Preparation:Enzymatic digestion of mouse brain followed by myelin removal is recommended to achieve the highest purity and yield. There are several protocols published that can be applied. As a general guideline, Trypsin digestion followed by a 70/37/30% percoll gradient will increase final purity and yield.Scale up volumes if using 14 ml tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-selection-kits-protocol-5/4244/ | 1 | 2019 | Sam Li 2019. MojoSort™ Selection Kits Protocol - 5. protocols.io https://dx.doi.org/10.17504/protocols.io.7z9hp96 | 2021-04-15 09:16:08 | ||
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Whole Mouse Brain Processing for Microglia Isolation, Cell Separation, and Flow Cytometry Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.97dh9i6 | BioLegend | BioLegend | https://www.biolegend.com/en-us/protocols/whole-mouse-brain-processing-for-microglia-isolation-cell-separation-and-flow-cytometry | 1 | 2019 | Sam Li 2019. Whole Mouse Brain Processing for Microglia Isolation, Cell Separation, and Flow Cytometry. protocols.io https://dx.doi.org/10.17504/protocols.io.97dh9i6 | 2021-04-15 09:16:09 | |||
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MojoSort™ Mouse CD11c Nanobeads Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7abhian | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-cd11c-nanobeads-column-protocol/4768/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse CD11c Nanobeads Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7abhian | 2021-04-15 09:16:15 | ||
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Intracellular Staining With True-Phos™ Perm Buffer in Cell Suspensions Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bac4iayw | BioLegend | BioLegend | https://www.biolegend.com/protocols/intracellular-staining-with-true-phos-perm-buffer-in-cell-suspensions-protocol/4262/ | 2 | 2019 | Sam Li 2019. Intracellular Staining With True-Phos™ Perm Buffer in Cell Suspensions. protocols.io https://dx.doi.org/10.17504/protocols.io.bac4iayw | 2021-04-15 09:16:33 | |||
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MojoSort™ Human CD45 Nanobeads Protocol 2 - CD45 greater than 50% Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7wqhpdw | BioLegend | Product description and procedure summary:If the percentage of CD45+ cells in your sample is less than 50%, please follow Human CD45 Nanobeads Protocol 1. If it is higher than 50% then please follow Human CD45 Nanobeads Protocol 2. The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-cd45-nanobeads-protocol-2-cd45-greater-than-50/4277/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human CD45 Nanobeads Protocol 2 - CD45 greater than 50%. protocols.io https://dx.doi.org/10.17504/protocols.io.7wqhpdw | 2021-04-15 09:16:18 | ||
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MojoSort™ Mouse Neutrophil Isolation Kit Column Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7bjhikn | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-neutrophil-isolation-kit-column-protocol/4770/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse Neutrophil Isolation Kit Column Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7bjhikn | 2021-04-15 09:16:25 | ||
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Anti-BrdU Staining Protocols Using DNAse with Surface and Fluorescent Proteins Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bac9iaz6 | BioLegend | BioLegend | https://www.biolegend.com/protocols/anti-brdu-staining-using-dnase-with-surface-and-fluorescent-proteins/4241/ | 3 | 2019 | Sam Li 2019. Anti-BrdU Staining Protocols Using DNAse with Surface and Fluorescent Proteins. protocols.io https://dx.doi.org/10.17504/protocols.io.bac9iaz6 | 2021-04-15 09:16:25 | |||
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MojoSort™ Nanobeads Column Protocol - 1 Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.69dhh26 | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-nanobeads-column-protocol-1/4593/ | 1 | 2019 | Sam Li 2019. MojoSort™ Nanobeads Column Protocol - 1. protocols.io https://dx.doi.org/10.17504/protocols.io.69dhh26 | 2021-04-15 09:16:27 | ||
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MojoSort™ Mouse CD11c Nanobeads Protocol Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7xjhpkn | BioLegend | Product description and procedure summary: The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. Sample Preparation: Enzymatic digestion of mouse spleen is recommended to achieve the highest purity and yield of CD11c+ cells. There are several protocols published that can be applied. As a general guideline, cut mouse spleen into pieces and incubate in 0.5 mg/ml Collagenase for 30 to 60 minutes at room temperature or 37°C. Place the tube in a rocking platform with continuous agitation or gently pipette every 10 minutes. Alternatively, inject 1 ml of enzymes solution in the uncut organ. Force the tissue through a 70µm filter to prepare a single cell suspension, and wash with complete media. Resuspend cells in 0.1 mg/ml DNase 1 solution and incubate at room temperature for 10 minutes. Again, filter cells through a 70 µm filter and wash with complete media. Resuspend in complete media or MojoSort™ Buffer and keep on ice until ready to use. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-cd11c-nanobeads-protocol/4401/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse CD11c Nanobeads Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.7xjhpkn | 2021-04-15 09:16:27 | ||
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Immunohistochemistry Protocol for Paraffin-Embedded Sections Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.97bh9in | BioLegend | BioLegend | https://www.biolegend.com/protocols/immunohistochemistry-protocol-for-paraffin-embedded-sections/4256/ | 3 | 2019 | Sam Li 2019. Immunohistochemistry Protocol for Paraffin-Embedded Sections. protocols.io https://dx.doi.org/10.17504/protocols.io.97bh9in | 2021-04-15 09:16:31 | |||
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MojoSort™ Human CD4 T Cell Selection Kit Column Protocol - CD4 Nanobeads Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.7tyhnpw | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-cd4-t-cell-selection-kit-column-protocol-cd4-nanobeads/4762/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human CD4 T Cell Selection Kit Column Protocol - CD4 Nanobeads. protocols.io https://dx.doi.org/10.17504/protocols.io.7tyhnpw | 2021-04-15 09:17:02 | ||
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True-Nuclear™ Transcription Factor Staining Protocol for 96-Well, U-Bottom Plate Resource Report Resource Website |
Sam Li | DOI:10.17504/protocols.io.bacriav6 | BioLegend | BioLegend | https://www.biolegend.com/protocols/true-nuclear-transcription-factor-staining-protocol-for-96-well-u-bottom-plate/4246/ | 5 | 2019 | Sam Li 2019. True-Nuclear™ Transcription Factor Staining Protocol for 96-Well, U-Bottom Plate. protocols.io https://dx.doi.org/10.17504/protocols.io.bacriav6 | 2021-04-15 09:17:00 |
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