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Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:Hyperinsulinemic-euglycemic clamp is the gold-standard method to assess insulin sensitivity. The hyperinsulinemic-euglycemic clamp is widely used in clinics and laboratories to measure insulin action on glucose utilization in humans and animals for clinical and basic science research. Incorporation of radioactive-labeled glucose during hyperinsulinemic-euglycemic clamps makes it possible to measure glucose metabolism in individual organs in awake mice. Impaired insulin sensitivity (insulin resistance) is a major characteristic of obesity and an early requisite event in the development of type 2 diabetes.
Proper citation: Jason Kim 2019. U Mass - Hyperinsulinemic-euglycemic clamp. protocols.io dx.doi.org/10.17504/protocols.io.xzefp3e Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of HDL-cholesterol reflect cholesterol metabolism and are associated with cardiovascular disease.
Proper citation: Jason Kim 2019. U Mass - Cholesterol (HDL). protocols.io dx.doi.org/10.17504/protocols.io.xt6fnre Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment provides the quantification of multiple hormones using multiplexed-Luminex technology based on beads containing specific antibodies. Sample matrices include plasma, serum, tissue culture supernatants, and tissue or cell lysates. Serum glucagon levels reflect glucagon secretion and pancreatic α-cell function. Serum glucagon levels regulate hepatic glucose production and glucose homeostasis. Serum glucagon levels are altered in obesity, insulin resistance, and counterregulatory stress response.
Proper citation: Jason Kim 2019. U Mass - Glucagon. protocols.io dx.doi.org/10.17504/protocols.io.xw3fpgn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Intravenous administration via tail vein is used to acutely deliver drug, hormones, and adenoassociated virus in mice. A large fraction of injectate will be cleared by liver.
Proper citation: Jason Kim 2019. U Mass - Surgery – tail vein injection. protocols.io dx.doi.org/10.17504/protocols.io.x5dfq26 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum lactate levels are affected by alterations in glucose and protein metabolism.
Proper citation: Jason Kim 2019. U Mass -Lactate. protocols.io dx.doi.org/10.17504/protocols.io.x5ufq6w Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: The adiponectin enzyme-linked immunosorbent assay (ELISA) is a standard immunoassay for the detection of total adiponectin levels in serum, adipocyte extracts, and cell culture media samples. This experiment uses a 96-well plate coated with anti-adiponectin antibodies and developed with a secondary anti-adiponectin antibody linked to horseradish peroxidase. The ELISA is developed with 3,3',5,5'-Tetramethylbenzidine (TMB), a chromogenic substrate and read on an automated plate spectrophotometer at 450 nm. Adiponectin is an adipocyte-derived hormone (adipokine) that regulates insulin sensitivity and glucose metabolism.
Proper citation: Jason Kim 2019. U Mass - Adiponectin. protocols.io dx.doi.org/10.17504/protocols.io.xm9fk96 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:
This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity.
Cytokines Panel III include IL-20 (interleukin-20), IL-23, IL-27, IL-33, MDC (macrophage- derived chemokine; CCL-22), and TIMP-1 (tissue inhibitor of metalloproteinases).
A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay.
Proper citation: Jason Kim 2019. U Mass - Cytokines Panel III - multiplex. protocols.io dx.doi.org/10.17504/protocols.io.xv7fn9n Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Glucose uptake in individual organs can be measured using a bolus injection of 2-deoxy-D-[1-14C] glucose, a non-metabolizable glucose analog, and by determining labeled metabolite levels in select tissues. Insulin resistance is characterized by reduced glucose metabolism and develops in obese mice.
Proper citation: Jason Kim 2019. U Mass - Organ-specific glucose uptake. protocols.io dx.doi.org/10.17504/protocols.io.x4cfqsw Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of LDL-cholesterol reflect cholesterol metabolism and are associated with cardiovascular disease.
Proper citation: Jason Kim 2019. U Mass - Cholesterol (LDL). protocols.io dx.doi.org/10.17504/protocols.io.xt9fnr6 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:Chronic indwelling catheter is placed in the jugular vein for intravenous infusion and injection of drug, hormones, and metabolites. Surival surgery is performed in anesthetized mice, and mice recover from surgery after 4~5 days.
Proper citation: Jason Kim 2019. U Mass - Surgery – jugular vein cannulation. protocols.io dx.doi.org/10.17504/protocols.io.x5cfq2w Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer.
Proper citation: Jason Kim 2019. U Mass - Creatine Kinase. protocols.io dx.doi.org/10.17504/protocols.io.xumfnu6 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of alkaline phosphatase reflect liver function.
Proper citation: Jason Kim 2019. U Mass - Alkaline Phosphatase. protocols.io dx.doi.org/10.17504/protocols.io.xptfmnn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer.
Proper citation: Jason Kim 2019. U Mass - Ammonia. protocols.io dx.doi.org/10.17504/protocols.io.xq3fmyn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer.
Proper citation: Jason Kim 2019. U Mass - Amylase. protocols.io dx.doi.org/10.17504/protocols.io.xq4fmyw Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum alanine transferase levels reflect liver function.
Proper citation: Jason Kim 2019. U Mass - Alanine Transferase. protocols.io dx.doi.org/10.17504/protocols.io.xpmfmk6 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment provides the quantification of multiple hormones using multiplexed-Luminex technology based on beads containing specific antibodies. Sample matrices include plasma, serum, tissue culture supernatants, and tissue or cell lysates. Resistin is an adipocyte-derived hormone (adipokine) that regulates insulin sensitivity and glucose metabolism. Serum resistin levels are altered in obesity, insulin resistance, and type 2 diabetes.
Proper citation: Jason Kim 2019. U Mass - Resistin. protocols.io dx.doi.org/10.17504/protocols.io.x4kfquw Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of total cholesterol reflect cholesterol metabolism and are associated with cardiovascular disease.
Proper citation: Jason Kim 2019. U Mass - Cholesterol (Total). protocols.io dx.doi.org/10.17504/protocols.io.xucfnsw Copy
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