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Authors: G-Biosciences
Group: G-Biosciences
Summary: This protocol is part of the FOCUS™ Mitochondria Kit collection. Please refer to the appropriate protocol depending on your application.For facilitating homogenization of the hard tissue, 0.25mg/ml Trypsin should be added to 1X SubCell Buffer-II. A concentrated BSA solution is needed to quench the proteolytic reaction after Trypsin treatment.
Proper citation: G-Biosciences 2016. Isolation of Mitochondria from Hard Tissues (Skeletal or Heart Muscle) using the FOCUS™ Mitochondria Kit. protocols.io https://dx.doi.org/10.17504/protocols.io.e9bbh2n Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.This protocol is for use with gram-negative bacteria. Please refer to the appropriate protocol depending on your application.
Proper citation: G-Biosciences 2016. OmniPrep™ For High Quality Genomic DNA Extraction From Gram-Negative Bacteria. protocols.io https://dx.doi.org/10.17504/protocols.io.e56bg9e Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: The Non-Interfering™ Protein Assay is a colorimetric assay for determining protein concentrations in protein loading buffer (Laemmli buffer), high β-mercaptoethanol concentrations, and in lipid and vesicle preparations.
Proper citation: G-Biosciences 2016. A Non-Interfering™ (NI) Protein Assay (high throughput 96-well). protocols.io https://dx.doi.org/10.17504/protocols.io.e9tbh6n Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.This protocol is for use with gram-positive bacteria. Please refer to the appropriate protocol depending on your application.
Proper citation: G-Biosciences 2016. OmniPrep™ For High Quality Genomic DNA Extraction From Gram-Positive Bacteria. protocols.io https://dx.doi.org/10.17504/protocols.io.e57bg9n Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.This protocol is for use with fungal tissue. Please refer to the appropriate protocol depending on your application.
Proper citation: G-Biosciences 2016. OmniPrep™ For High Quality Genomic DNA Extraction From Fungal Tissue. protocols.io https://dx.doi.org/10.17504/protocols.io.e6gbhbw Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: The UPPA-PROTEIN Concentrate™ kit is used to quantitatively concentrate dilute protein samples as low as 1ng/ml into a small volume. Protein precipitation and concentration are not affected by the presence of detergents, chaotropics, or other common laboratory agents.
Proper citation: G-Biosciences 2017. UPPA-PROTEIN Concentrate™. protocols.io https://dx.doi.org/10.17504/protocols.io.e5gbg3w Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: This is a collection of G-Biosciences protocols: SpinOUT™ for Desalting & Buffer Exchange of Peptide & Protein Samples.(Cat. # 786-170 to 786-173, 786-703 to 786-708, 786-865 to 786-869)
Proper citation: G-Biosciences 2016. SpinOUT™ for Desalting and Buffer Exchange of Peptide and Protein Samples. protocols.io https://dx.doi.org/10.17504/protocols.io.fkibkue Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: SpinOUT™ protocol for buffer exchange of peptide and protein samples.(Cat. # 786-170 to 786-173, 786-703 to 786-708, 786-865 to 786-869)
Proper citation: G-Biosciences 2016. SpinOUT™: Buffer Exchange. protocols.io https://dx.doi.org/10.17504/protocols.io.e5hbg36 Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: This is a collection of CasPASE™ Apoptosis Fluorometic Assay protocols from G-Biosciences. Please follow the appropriate protocol, depending on your type of lysate.
Proper citation: G-Biosciences 2017. CasPASE™ Apoptosis Fluorometic Assay Protocols. protocols.io https://dx.doi.org/10.17504/protocols.io.gybbxsn Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: Chemiluminescence Detection System for Horseradish Peroxidase
Proper citation: G-Biosciences 2017. picoLUCENT™ PLUS?HRP. protocols.io https://dx.doi.org/10.17504/protocols.io.e8cbhsw Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: This is part of the collection of FOCUS™ SubCell protocols for the enrichment of subcellular fractions. Please refer to the appropriate protocol depending on your application.
Proper citation: G-Biosciences 2016. Cleaning of the Nuclear Fraction (FOCUS™ SubCell Kit). protocols.io https://dx.doi.org/10.17504/protocols.io.e9fbh3n Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: MegaLong™ isolates high molecular weight (>100kb) genomic DNA from a variety of samples, including animal tissues, cultured cells, whole blood, bacterial and yeast.The protocol described here is for bacteral DNA samples. Please refer to the appropriate protocol, depending on your application.
Proper citation: G-Biosciences 2016. MegaLong™ Protocol for Isolation of >100kb Genomic DNA (Bacterial DNA). protocols.io https://dx.doi.org/10.17504/protocols.io.e8pbhvn Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: SpinOUT™ protocol for desalting of peptide and protein samples.(Cat. # 786-170 to 786-173, 786-703 to 786-708, 786-865 to 786-869)
Proper citation: G-Biosciences 2016. SpinOUT™: Protein Desalting. protocols.io https://dx.doi.org/10.17504/protocols.io.e4hbgt6 Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: MegaLong™ isolates high molecular weight (>100kb) genomic DNA from a variety of samples, including animal tissues, cultured cells, whole blood, bacterial and yeast.The protocol described here is for cell culture samples. Please refer to the appropriate protocol, depending on your application.
Proper citation: G-Biosciences 2016. MegaLong™ Protocol for Isolation of >100kb Genomic DNA (Cell Culture). protocols.io https://dx.doi.org/10.17504/protocols.io.e8kbhuw Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: The Non-Interfering™ Protein Assay is a colorimetric assay for determining protein concentrations in protein loading buffer (Laemmli buffer), high β-mercaptoethanol concentrations, and in lipid and vesicle preparations.
Proper citation: G-Biosciences 2016. A Non-Interfering™ (NI) Protein Assay. protocols.io https://dx.doi.org/10.17504/protocols.io.e9sbh6e Copy
Authors: G-Biosciences
Group: G-Biosciences, Plantae
Summary: The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.This protocol is for use with plant tissue. Please refer to the appropriate protocol depending on your application.
Proper citation: G-Biosciences 2016. OmniPrep™ For High Quality Genomic DNA Extraction From Plant Tissue (Fresh or Frozen). protocols.io https://dx.doi.org/10.17504/protocols.io.e6dbha6 Copy
Authors: G-Biosciences
Group: G-Biosciences
Summary: This is a collection of Non-Interfering™ (NI) Protein Assay protocols from G-Biosciences.
Proper citation: G-Biosciences 2016. Non-Interfering™ (NI) Protein Assay Protocols. protocols.io https://dx.doi.org/10.17504/protocols.io.fp3bmqn Copy
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