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Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol is for running a fluorescence titering assay for lentivirus. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. Fluorescence Titering Assay for Lentivirus. protocols.io dx.doi.org/10.17504/protocols.io.4xrgxm6 Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol describes plasmid cloning by restriction enzyme digest (aka subcloning). To see the full abstract and additional resources, please visit the Addgene protocol page.
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning). protocols.io dx.doi.org/10.17504/protocols.io.bawnifde Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol describes plasmid cloning by restriction enzyme digest (aka subcloning). To see the full abstract and additional resources, please visit the Addgene protocol page.
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning). protocols.io dx.doi.org/10.17504/protocols.io.47ugznw Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol is for running a colony formation titering assay for Lentivirus. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. Colony Formation Titering Assay for Lentivirus. protocols.io dx.doi.org/10.17504/protocols.io.47mgzk6 Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol is for generating stable cell lines with lentivirus. To see the full abstract and additional resources, please visit the Addgene protocol page.Sample Data
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. Generating Stable Cell Lines with Lentivirus. protocols.io dx.doi.org/10.17504/protocols.io.4xtgxnn Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: Please see the Addgene website for additional details.
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. Restriction Digest of Plasmid DNA. protocols.io dx.doi.org/10.17504/protocols.io.5jeg4je Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol is used for AAV production in HEK293T cells. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. AAV Production in HEK293T Cells. protocols.io dx.doi.org/10.17504/protocols.io.bawpifdn Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol is for performing Polymerase Chain Reaction (PCR). To see the full abstract and additional resources, visit https://www.addgene.org/protocols/pcr/.Basic PCR ProgramInitial Denaturation for at : This initiation step heats the double stranded DNA template strand to the point where the strands start denaturing and the hydrogen bonds are broken between the nucleotide base pairs.Denature at : Continued denaturation of double stranded DNA.Anneal primers for at : The forward and reverse primers are stable within this temperature range to anneal to each of the single stranded DNA template strands. The DNA polymerase is also stable enough to now bind to the primer DNA sequence.Extend DNA for at : The Taq polymerase has an optimal temperature around - so this step enables the DNA polymerase to synthesize and elongate the new target DNA strand accurately and rapidly.Repeat steps 2-5, 25-30 times.Final Extension for at : A final extension to fill-in any protruding ends of the newly synthesized strands.
Proper citation: Addgene The Nonprofit Plasmid Repository 2020. Polymerase Chain Reaction (PCR). protocols.io dx.doi.org/10.17504/protocols.io.4wggxbw Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol is for running a fluorescence titering assay for lentivirus. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. Fluorescence Titering Assay for Lentivirus. protocols.io dx.doi.org/10.17504/protocols.io.bawtifen Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: The following protocol is for making LB agar plates for the purpose of bacterial selection (500mL of LB agar makes about 25 LB agar plates). Please see the Addgene website for additional details.
Proper citation: Addgene The Nonprofit Plasmid Repository 2014. LB Agar Plates. protocols.io dx.doi.org/10.17504/protocols.io.cdzs75 Copy
Authors: Addgene The Nonprofit Plasmid Repository, Ken Christensen
Summary: The following protocol is for making LB agar plates for the purpose of bacterial selection (500mL of LB agar makes about 25 LB agar plates). For the full abstract and additional resources, please visit https://www.addgene.org/plasmid_protocols/bacterial_plates/
Proper citation: Addgene The Nonprofit Plasmid Repository, Ken Christensen 2020. Pouring LB Agar Plates - Chem 584. protocols.io dx.doi.org/10.17504/protocols.io.bjvwkn7e Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol is for general transfection. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. General Transfection. protocols.io dx.doi.org/10.17504/protocols.io.bawuifew Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol is for general transfection. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. General Transfection. protocols.io dx.doi.org/10.17504/protocols.io.4xqgxmw Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol is for inoculating a liquid bacterial culture. To see the full abstract and additional resources, visit https://www.addgene.org/protocols/inoculate-bacterial-culture/.
Proper citation: Addgene The Nonprofit Plasmid Repository 2020. Inoculating a Liquid Bacterial Culture. protocols.io dx.doi.org/10.17504/protocols.io.5abg2an Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol is for creating bacterial glycerol stocks for long-term storage of plasmids. To see the full abstract and other resources, visit the Addgene protocol page.
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids. protocols.io dx.doi.org/10.17504/protocols.io.4g5gty6 Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This is the protocol accompanying the "pLKO.1 – TRC Cloning Vector". For information about the PLKO.1-TRC cloning vector and tips on designing shRNA oligos for pLKO.1 see Addgene's website: http://www.addgene.org/tools/protocols/plko/
Proper citation: Addgene The Nonprofit Plasmid Repository 2014. Cloning shRNA Oligos into pLKO.1. protocols.io dx.doi.org/10.17504/protocols.io.cdus6v Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol is used for AAV production in HEK293T cells. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. AAV Production in HEK293T Cells. protocols.io dx.doi.org/10.17504/protocols.io.47igzke Copy
Authors: Addgene The Nonprofit Plasmid Repository
Group: Coronavirus Method Development Community
Summary: This protocol describes RNA extraction without a kit. To see the full abstract and additional resources, visit https://www.addgene.org/protocols/kit-free-rna-extraction/
Proper citation: Addgene The Nonprofit Plasmid Repository 2020. RNA Extraction Without a Kit. protocols.io dx.doi.org/10.17504/protocols.io.beabjaan Copy
Authors: Joanne Kamens, Addgene The Nonprofit Plasmid Repository
Group: Re-Entering Labs Post COVID-19 Shutdown
Proper citation: Joanne Kamens, Addgene The Nonprofit Plasmid Repository 2020. COVID-19 Addgene Operating Procedures. protocols.io dx.doi.org/10.17504/protocols.io.bk9vkz66 Copy
Authors: Addgene The Nonprofit Plasmid Repository
Summary: This protocol is for generating stable cell lines with lentivirus. To see the full abstract and additional resources, please visit the Addgene protocol page.Sample Data
Proper citation: Addgene The Nonprofit Plasmid Repository 2019. Generating Stable Cell Lines with Lentivirus. protocols.io dx.doi.org/10.17504/protocols.io.bawvife6 Copy
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