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| Name | Authors | DOI | Group |
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ELISA for quantification of IL-16 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj3gkqjw | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Eosinophils and CD8+T cells synthesize IL-16. Its principal target is CD4+ T cells. It causes CD4+ T cell chemoattraction. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-16 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj3gkqjw | 2021-03-29 03:08:33 | |||
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ELISA for quantification of IL-5 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj25kqg6 | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. They have both paracrine and autocrine function. Interleukins are also used in animal studies to investigate aspect related to clinical medicine.T cells and stem cells make IL-3. It functions as a multilineage colony-stimulating factor. CD4+T cells (Th2) produce IL-5, and its principal targets are B cells. It causes B-cell growth factor and differentiation and IgA selection. Besides, causes eosinophil activation and increased production of these innate immune cells [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-5 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj25kqg6 | 2021-03-29 03:08:38 | |||
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Universal sandwich ELISA for investigating the binding of avian immunoglobulins to Staphylococcal protein-A (SpA) using anti-IgY-peroxidase conjugate. Resource Report Resource Website |
Angel Justiz-Vaillant, Monica F. Smikle | 10.17504/protocols.io.bjqxkmxn | University of the West Indies, [email protected] | University of the West Indies St. Augustine, University of the West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Monica F. Smikle 2020. Universal sandwich ELISA for investigating the binding of avian immunoglobulins to Staphylococcal protein-A (SpA) using anti-IgY-peroxidase conjugate.. protocols.io dx.doi.org/10.17504/protocols.io.bjqxkmxn | 2021-03-29 03:08:42 | ||||
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Enzyme linked immunosorbent assay for investigating the binding of protein-LG (SpLG) to immunoglobulins. Resource Report Resource Website |
Angel Justiz-Vaillant, Norma McFarlane-Anderson | 10.17504/protocols.io.bjpmkmk6 | University of the West Indies, [email protected] | This SpLG ELISA can be used to detect specific antibodies in various animal species including human, mouse, rat, dog, rabbit, chicken, monkey, pig and hamster [1].1. Kihlberg BM, Sjöbring U, Kastern W, Björck L. Protein LG: a hybrid molecule with unique immunoglobulin binding properties.J Biol Chem. 1992;267(35):25583-25588. | University of the West Indies St. Augustine, University of West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Enzyme linked immunosorbent assay for investigating the binding of protein-LG (SpLG) to immunoglobulins.. protocols.io dx.doi.org/10.17504/protocols.io.bjpmkmk6 | 2021-03-29 03:08:39 | |||
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Isolation of Salmonella pathogens from oysters Resource Report Resource Website |
Sade Aisha Folashade John, Patrick E. Akpaka, Chandrashekhar Unakal, Arvind Kurhade, Angel Justiz-Vaillant | 10.17504/protocols.io.bj6qkrdw | University of the West Indies, [email protected] | University of the West Indies St. Augustine, University of the West Indies St. Augustine, University of the West Indies St. Augustine, University of the West Indies St. Augustine, University of the West Indies. St. Augustine | 1 | 2020 | Sade Aisha Folashade John, Patrick E. Akpaka, Chandrashekhar Unakal, Arvind Kurhade, Angel Justiz-Vaillant 2020. Isolation of Salmonella pathogens from oysters. protocols.io dx.doi.org/10.17504/protocols.io.bj6qkrdw | 2021-03-29 03:08:39 | ||||
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ELISA for quantification of IL-9 in human serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bkevkte6 | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Th9, Th2, Th17, mast cells, NKT cells, and regulatory T cells produce interleukin-9. It enhances T-cell survival, mast cell activation and synergy with erythropoietin.[1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-9 in human serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bkevkte6 | 2021-03-29 03:09:00 | |||
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ELISA for quantification of IL-19 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj3jkqkn | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Th2 lymphocytes synthesize IL-19 and acts on resident vascular cells in addition to immune cells. It is an anti-inflammatory molecule. It promotes immune responses mediated by regulatory lymphocytes and has substantial activity on microvascular.[1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-19 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj3jkqkn | 2021-03-29 03:09:00 | |||
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Enzyme linked immunosorbent assay for investigating the binding of Protein-AG (SpAG) to immunoglobulins. Resource Report Resource Website |
Angel Justiz-Vaillant, Norma McFarlane-Anderson | 10.17504/protocols.io.bjppkmmn | University of the West Indies, [email protected] | This SpAG ELISA can be used to detect specific antibodies in various animal species including human, goat, donkey, mouse, rat, dog, cow, horse, rabbit, chicken, monkey, pig and hamster [1].1. Kelly PJ, Tagwira M, Matthewman L, Mason PR, Wright EP. Reactions of sera from laboratory, domestic and wild animals in Africa with protein A and a recombinant chimeric protein AG.Comp Immunol Microbiol Infect Dis. 1993;16(4):299-305. doi:10.1016/0147-9571(93)90159-3 | University of the West Indies St. Augustine, University of West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Norma McFarlane-Anderson 2020. Enzyme linked immunosorbent assay for investigating the binding of Protein-AG (SpAG) to immunoglobulins.. protocols.io dx.doi.org/10.17504/protocols.io.bjppkmmn | 2021-03-29 03:09:05 | |||
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Universal sandwich ELISA for investigating the binding of Protein-LA (SpLA) to avian immunoglobulins using a peroxidase-labeled -anti-IgY conjugate. Resource Report Resource Website |
Angel Justiz-Vaillant, Monica F. Smikle | 10.17504/protocols.io.bjq2kmye | University of the West Indies, [email protected] | University of the West Indies St. Augustine, University of the West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Monica F. Smikle 2020. Universal sandwich ELISA for investigating the binding of Protein-LA (SpLA) to avian immunoglobulins using a peroxidase-labeled -anti-IgY conjugate.. protocols.io dx.doi.org/10.17504/protocols.io.bjq2kmye | 2021-03-29 03:09:19 | ||||
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Competitive enzyme-linked immunosorbent assay for investigating SpL binding to mammalian and avian immunoglobulins Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjqckmsw | University of the West Indies, [email protected] | This ELISA was based on the theory that antibodies present in different samples would compete with human IgG for binding to SpL, resulting in inhibition of human IgG-SpL interactions [1].Reference:1. Justiz-Vaillant AA, Akpaka PE, McFarlane-Anderson N, Smikle MF. Comparison of techniques of detecting immunoglobulin-binding protein reactivity to immunoglobulin produced by different avian and mammalian species.West Indian Med J. 2013;62(1):12-20. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Competitive enzyme-linked immunosorbent assay for investigating SpL binding to mammalian and avian immunoglobulins. protocols.io dx.doi.org/10.17504/protocols.io.bjqckmsw | 2021-03-29 03:09:24 | |||
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Detection of anti- keyhole limpet hemocynin (anti-KLH) antibodies by double immunodiffusion (Ouchterlony) technique. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjspkndn | University of the West Indies, [email protected] | Keyhole limpet hemocyanin (KLH) is a cooper-containing protein comprising of subunits with MW of 400 kDa. This protein is found in the hemolymph of the sea mollusk Megathura crenulata. It has the ability to enhance the host’s immune response by interacting with monocytes, T cells and macrophages. KLH has been used primarily as a carrier for vaccines and antigens [1]. It was found that chicken immunized with KLH bound peptide raised an anti-KLH immunoresponse [2]. This can be tested by a single method such as the Ouchterlony technique.Reference1. Aarntzen EH, de Vries IJ, Göertz JH, et al. Humoral anti-KLH responses in cancer patients treated with dendritic cell-based immunotherapy are dictated by different vaccination parameters.Cancer Immunol Immunother. 2012;61(11):2003-2011. doi:10.1007/s00262-012-1263-z2. Justiz Vaillant AA, Anderson MF, Smikle M, Wisdom B, Mohammed W, et al. (2013) Development of Anti HIV Gp120 and HIV Gp41 Peptide Vaccines. J Vaccines Vaccin 4: 206. doi: 10.4172/2157-7560.1000206 | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Detection of anti- keyhole limpet hemocynin (anti-KLH) antibodies by double immunodiffusion (Ouchterlony) technique.. protocols.io dx.doi.org/10.17504/protocols.io.bjspkndn | 2021-03-29 03:11:57 | |||
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ELISA for quantification of IL-31 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj34kqqw | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. IL-31 is produced mainly by Th2 cells and dendritic cells. It is a proinflammatory cytokine and a chemotactic factor that direct polymorphonuclear cells, monocytes, and T cells to inflammatory lesions. IL-31 induces chemokines production and synthesis of IL-6, IL-16, and IL-32. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-31 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj34kqqw | 2021-03-29 03:11:58 | |||
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ELISA for quantification of IL-19 in human serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bkeqktdw | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Th2 lymphocytes synthesize IL-19 and acts on resident vascular cells in addition to immune cells. It is an anti-inflammatory molecule. It promotes immune responses mediated by regulatory lymphocytes and has substantial activity on microvascular.[1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-19 in human serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bkeqktdw | 2021-03-29 03:12:01 | |||
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Determination of C4 concentration by the Mancini test. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj95kr86 | University of the West Indies, [email protected] | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Determination of C4 concentration by the Mancini test.. protocols.io dx.doi.org/10.17504/protocols.io.bj95kr86 | 2021-03-29 03:12:01 | ||||
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Preparation of a protein-AG conjugated to horseradish peroxidase by the periodate method. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjk6kkze | University of the West Indies, [email protected] | A recombinant protein that combines the IgG-binding domains of SpA and SpG was developed, and labelled to horseradish peroxidase. It was used as universal conjugate in ELISA for the assessment of antibodies against Brucella spp in cattle, sheep, dogs, goats and pigs. It was reported that similar results as the one shown using the chimeric protein AG were obtained when murine monoclonal antibody-enzyme conjugates were used [1,2]. References1. Justiz-Vaillant AA, Akpaka PE, McFarlane-Anderson N, Smikle MF. Comparison of techniques of detecting immunoglobulin-binding protein reactivity to immunoglobulin produced by different avian and mammalian species.West Indian Med J. 2013;62(1):12-20.2. Justiz-Vaillant AA, McFarlane-Anderson N, and Smikle M. “Bacterial Immunoglobulin (Ig)-Receptors: Past and Present Perspectives.” American Journal of Microbiological Research, vol. 5, no. 2 (2017): 44-50. doi: 10.12691/ajmr-5-2-4. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Preparation of a protein-AG conjugated to horseradish peroxidase by the periodate method.. protocols.io dx.doi.org/10.17504/protocols.io.bjk6kkze | 2021-03-29 03:07:51 | |||
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Purification of the any avian IgY using chloroform, trichloroacetic acid and affinity purification using HiTrap™ Columns. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bjnskmee | University of the West Indies, [email protected] | This IgY purification technology can be used to purified IgY from the egg yolk of any bird as ducks, bantam hens, ostrich, chicken, pigeon, pheasant, quail, guinea hen, cattle egret and goose. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. Purification of the any avian IgY using chloroform, trichloroacetic acid and affinity purification using HiTrap™ Columns.. protocols.io dx.doi.org/10.17504/protocols.io.bjnskmee | 2021-03-29 03:07:44 | |||
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ELISA for quantification of human C6 in serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | 10.17504/protocols.io.bj8bkrsn | University of the West Indies, [email protected] | University of the West Indies St. Augustine, "Saturnino Lora Torres' Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human C6 in serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bj8bkrsn | 2021-03-29 03:08:01 | ||||
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ELISA for quantification of IL-37 in human serum. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bj4bkqsn | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. IL-37 plays an essential role in the regulation of the innate immunity causing immunosuppression. Phagocytes and organs including the uterus, testis, and thymus express it. IL-37 upregulates immune responses and inflammation in autoimmune disorders. [1]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-37 in human serum.. protocols.io dx.doi.org/10.17504/protocols.io.bj4bkqsn | 2021-03-29 03:08:14 | |||
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ELISA for quantification of human C9 in serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant, Belkis Ferrer-Cosme | 10.17504/protocols.io.bj79krr6 | University of the West Indies, [email protected] | University of the West Indies St. Augustine, "Saturnino Lora Torres' Provincial Teaching Clinical Surgical Hospital. Cuba | 1 | 2020 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human C9 in serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bj79krr6 | 2021-03-29 03:08:15 | ||||
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ELISA for quantification of IL-41 in human serum or plasma. Resource Report Resource Website |
Angel Justiz-Vaillant | 10.17504/protocols.io.bkeuktew | University of the West Indies, [email protected] | Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. [1]The immunoregulatory cytokine IL-41 (also known as meteorin-like protein) is expressed at high levels in the synovium of patients with psoriatic arthritis (PsA).[2]Reference1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.2.Onuora S. Novel cytokine, IL-41, linked with PsA.Nat Rev Rheumatol. 2019;15(11):636. doi:10.1038/s41584-019-0314-7 | University of the West Indies St. Augustine | 1 | 2020 | Angel Justiz-Vaillant 2020. ELISA for quantification of IL-41 in human serum or plasma.. protocols.io dx.doi.org/10.17504/protocols.io.bkeuktew | 2021-03-29 03:08:25 |
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